17 6 Vol.17 No.6 2010 11 Journal of Fishery Sciences of China November 2010 A C E 王际英 1, 李宝山 1, 马晶晶 1, 柳旭东 2, 宋志东 2, 苗淑彦 2, 李培玉 2, 王世信黄炳山 1 1, 张利民 (1., 264006; 2., 265500) 1, 摘要 : (Paralichthys olivaceus), ( ) A C E,, A, A (P<0.05); C (P<0.05), (P>0.05); E (P<0.05), (P<0.05),, A C E, A C (P>0.05), E (P<0.05), A C E,, E, A C, [, 2010,17(6): 1250 1256] : ; ; ; ; A; C; E 中图分类号 : S965.3 文献标识码 : A 文章编号 : 1005 8737 (2010)06 1250 07 (Paralichthys olivaceus), 8~24, 16~21 [1],,,,,,,, [2 4], [5-6] [7 11],, [12 14], A,, [15 16] ; C (Dicentrarchus labrax) (Sparus aurata), [17] ; E [18], A C E 收稿日期 : 2010 01 11; 修订日期 : 2010 02 05. 基金项目 : (nyhyzx07-046); ;. 作者简介 : (1965 ),,,. E-mail: ytwjy@126.com 通讯作者 : (1956 ),,,. E-mail: zhanglimin@126.com
6 : A C E 1251,,, [19 20] ( ) A C E,,, 1 1.1 2008 4, 40.5~ 75.5 cm, 973~5 129 g, 17.5~27.6 cm 8 2008 11 2009 1 2009 5, 41.0~ 66.0 cm, 913~4 185 g, 15.6~27.0 cm 10 ( ); 10 A C E ( ), : AD ( ), C ( ), E ( ) [21] Lavens [22] : A, 30 000 IU/kg ; C, 333 mg/kg ; E, 2 000 mg/kg 1, 1.2 8 10 10, 1,,, 80 1.3 A E : A E (GB12388-1990) C, :, 9 5%, 12 000 r/min 2 min, 2 740 g 6 min,, 0.22 μm, : C 18, 4.6 nm 250 nm 0.05 mol/l KH 2 PO 4 (ph 3.0), 0.6 ml/min, 254 nm, 30, 20 μl (Sigma), 1.4 EXCEL 2000 SPSS 11.0, ±, 0.05 (LSD ) 2 2.1 3 A C E A C E 1: A C (P> 0.05), E (P<0.05);, A C (P<0.05), Vitamins 表 1 野生 养殖与营养强化褐牙鲆肌肉中维生素 A C E 的含量 Tab. 1 V A,V C and V E contents in muscle of wild, farmed and fortified Paralichthys olivaceus x ±SD; µg/g (DW) Wild broodstock (n=8) Farmed broodstock Fortified broodstock V A 7.40±2.68 a 7.08±3.13 a 28.48±5.66 b V C 51.12±2.44 a 50.73±3.58 a 173.08±25.23 b V E 101.80±8.81 b 143.58±6.04 a 15.00±1.62 a : (P<0.05) Note: Values in the same line with different superscripts are significantly different (P<0.05).
1252 17 E (P<0.05) 2.2 3 A C E A C E 2, A (P>0.05), C E (P<0.05) A (P<0.05), 2.53 ; C 515.7%(P<0.05);, E (34.41~96.08 μg/g), E (P<0.05) 2.3 3 A C E A C E 3, A C (P<0.05), E 31.6%(P<0.05) E (P<0.05), A C (P> 0.05), (P<0.05) 3 A 3, A, A [23 24] A, A,, A, Furuita [25 26], A 16.9 mg/kg, A ; A(3 10 5 IU/100g), A 2, A Vitamins 表 2 野生 养殖与营养强化褐牙鲆肝脏中维生素 A C E 的含量 Tab. 2 V A,V C and V E contents in liver of wild, farmed and fortified Paralichthys olivaceus x ±SD; µg/g (DW) Wild broodstock(n=8) Farmed broodstock Fortified broodstock V A 55.66±2.08 a 53.76±1.16 a 135.86±4.89 b V C 209.09±8.13 b 160.57±4.91 a 988.61±34.36 c V E 127.81±2.93 c 34.41±1.33 a 96.08±10.54 b : (P<0.05) Note: Values in the same row with different superscripts are significantly different (P<0.05). Vitamins 表 3 野生 养殖与营养强化褐牙鲆卵中维生素 A C E 的含量 Tab. 3 V A,V C and V E contents in eggs of wild, farmed and fortified Paralichthys olivaceus x ±SD; µg/g (DW) Wild broodstock(n=8) Farmed broodstock Fortified broodstock V A 72.61±2.78 b 27.66±2.85 a 30.72±1.28 a V C 15230±1210 b 11180±2180 a 11460±3880 a V E 106.06±4.70 a 139.58±16.99 b 207.89±7.30 c : (P<0.05). Note: Values in the same row with different superscripts are significantly different (P<0.05).
6 : A C E 1253,, 2.53, Furuita [26] (SD; 16.90 mg/kg V A ) (CD; 0.77 mg/kg V A ) (1.48), A, Combs [27] Furuita [25] Furuita [25], A,, V A [25] A [25], A C DNA, C [28] C, C, C [29], [30],, C,, Blom [25] C, C, C, Lavens [22] C-2-, C C [22], C ( C-2- / C), C, C, C, Biswas [32] E,,,, E (Perca flavescens) [33], (Epinephelus coioides) [18], (Sparus aurata) [34], [35] (Chanos chanos) [36], E E [37], E ( ) E [38 41], E, E, E E E 179% 48.9%, E, Lewis-McCrea [42] E, A C E, A C E,,, A C E, 2 参考文献 : [1]. [D]. :, 2001: 2 3. [2],,,. 50DE
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1256 17 A comparison study of Vitamin A, C and E in wild and farmed Japanese flounder, Paralichthys olivaceus WANG Jiying 1, LI Baoshan 1, MA Jingjing 1, LIU Xudong 2, SONG Zhidong 2, MIAO Shuyan 2, LI Peiyu 2, WANG Shixin 1, HUANG Bingshan 1, ZHANG Limin 1 (1. Marine Fisheries Research Institute of Shandong Province, Yantai 264006, China; 2. Shengsuo Fishery Feed Research Centre of Shandong Province, Yantai 265500, China) Abstract: A comparison analysis of vitamin A, C and E contents in muscle, liver and eggs of wild, cultured and nutrition fortified Paralichthys olivaceus broodstock was conducted, attempting to explain the decline of larval fish quality in farmed Paralichthys olivaceus. The results showed that no differences were found in vitamin A content of wild and farmed fish, however, egg vitamin A content of wild fish was significantly higher than that of farmed fish (P<0.05). Vitamin C contents in liver and eggs of wild fish were statistically higher than those of farmed fish (P<0.05), while no change were detected in muscle. Vitamin E content in muscle and egg of wild fish were significantly higher than those of farmed fish, with liver vitamin E content following the opposite tendency. Fortification strategies in this study improved vitamin A and C contents in liver and muscle, vitamin E content in eggs of farmed broodstock, while vitamin A and C contents in eggs as well as vitamin E content in muscle changed a little (P>0.05). All the above results reached a conclusion that the discrepancy of vitamin A, C and E contents existed between wild and farmed fish, to some extent, may be correlated with the decline of the larval fish quality in the culture of Paralichthys olivaceus, and this could be diminished by more effective fortification strategies of vitamin A, C and E, which is needed to be further studied. [Journal of Fishery Sciences of China, 2010, 17(6): 1250 1256] Key words: Paralichthys olivaceus; wild Population; farmed; fortification; vitamin A; vitamin C; vitamin E Corresponding author: ZHANG Limin. E-mail: zhanglimin@126.com ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 欢迎订阅 2011 年 水产学报 ( CSCD ), 1964, 2002-2009, 2006-2008 (C ), 2009-2010 (B ), 160, 49.00, 588.00 () : CN 31-1283/S, : ISSN 1000-0615, : 4-297, : Q-387, 6 : 999 201 : 201306 : : 021-61900227 E-mail: mqzhang@shou.edu.cn; jfc@shou.edu.cn Website: www.scxuebao.cn