60 12 ( Pampus argenteus), [ 1 ], [ [ 2 ],, ( ) cm, ( ),, g] 1. 2,,, 50%d , ph 7. 9 [ 3 ], 0. 5, 6 8 mgl - 1, 27, 24 h [

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1 South China Fisheries Science Vol. 12, No. 2 Apr., 2016 doi: / j. issn , ,,,, ( 1.,, ; 2., ) : ( Pampus argenteus), 3 22, 27 32, 27, 48 h,, 2 ( GPT), ( GOT) ; 2 ( AKP), ( ACP), ( LDH) 22 ( P < 0. 05) ; ( Na + ) ( K + ) ( Cl - ) ( Ca 2 + ) ; Na + / K + -ATP 32, Na + /K + -ATP 22 ( P < 0. 05) ; 2 Ca 2 + / Mg 2 + -ATP Ca 2 + / Mg 2 + -ATP,,, : ; ; ; ; : S : A : ( 2016) Effect of acute temperature stress on metabolic enzymes, ion enzymes and concentration of ion in serum of juvenile Pampus argenteus GAO Quanxin 1, XIE Mingmei 1, 2, PENG Shiming 1, ZHANG Chenjie 1, SHI Zhaohong 1 ( 1. Key Lab. of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai , China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai , China) Abstract: To investigate the effect of acute temperature stress on biochemical indicators of juvenile Pampus argenteus, we designed two treatment groups ( 22 and 32 ) and a control group ( 27 ) with stress time of 42 h. The GPT in serum and GOT in liver in two experimental groups changed oppositely; AKP varied in an opposite manner but ACP changed in the same way; LDH in 22 group changed in a wave-like manner ( P < 0. 05). Na +, K +, Cl - and Ca 2+ concentrations in three groups changed differently. The Na + / K + -ATPase activity in gill in 32 group decreased, but that in kidney in 22 group increased first then decreased ( P < 0. 05). In two experimental groups, Ca 2 + / Mg 2 + -ATPase activities in gill and kidney varied in oppositely ( P < 0. 05). It is suggested that acute temperature stress, which should be avoided in breeding for juvenile P. argenteus, affects normal metabolismof the juveniles significantly. Key words: acute temperature stress; juvenile Pampus argenteus; metabolic enzyme; ion enzyme; density of ion in serum : ; : : ( ) ; ( 2014 Z02-1) : ( ),,,, gaoqx2008@ 163. com : ( ),,, shizh@ eastfishery. ac. cn

60 12 ( Pampus argenteus), [ 1 ], [ [ 2 ],, ( 9. 1 1. 1) cm, ( 18. 8 7. 2),, g] 1. 2,,, 50%d - 1 28 0. 5, ph 7. 9 [ 3 ], 0. 5, 6 8 mgl - 1, 27, 24 h [ 4-5 ], 22 27 32 3, 27, 3, 20, ( P <0.

2 60 12 ( Pampus argenteus), [ 1 ], [ [ 2 ],, ( ) cm, ( ),, g] 1. 2,,, 50%d , ph 7. 9 [ 3 ], 0. 5, 6 8 mgl - 1, 27, 24 h [ 4-5 ], , 27, 3, 20, ( P <0. 05) , 4 d, m 2. 5 m ( CW-2500A), 0 12 [ 6], ,,, 3, 9, 200 mgl - 1 MS-222 ( GOT) ( GPT),, ( AKP) ( ACP), 4 12 h, 4 ( LDH), rmin min, - 70,,,, - 20, [ 7-9 ] ATP, Na + /K + -ATP [ ph 7. 4, moll - 1 Tris-HCl, moll - 1 ( K + ) ( Na + ), Ca 2 + / Mg 2 + -ATP ( Ca 2 + [ 10 ] ),, Na + /K + -ATP Ca 2 + /Mg 2 + -ATP 4 15 min, Na + Ca 2 +, 1. 4 GPT, GOTAKPACPLDHNa + K + Ca 2 +, ( Cl - ) Na + /K + -ATP Ca 2 + / Mg 2 + -ATP,,,, EDTA-2Na, moll - 1, 0. 8% NaCl] rmin - 1 ( 7600 ) 1. 5 ( X SD), 2014 SPSS 19. 0,

2 : 61, 32,,,,, 2 24, Duncan s ; 12 ( P <, P < 0. 05 0. 05) ; 48 32 2 Excel 2010 2 2. 1 GPT GPT 32,, 48 2. 3 AKP ( P < 0. 05), 22, ( P < 0.

3 2 : 61, 32,,,,, 2 24, Duncan s ; 12 ( P <, P < ) ; Excel GPT GPT 32,, AKP ( P < 0. 05), 22, ( P < 0. 05) ( 1 - ) ; AKP, 48 ; GPT,, 32, 12, ( 3 - ) 12 ( P < 0. 05) ( 1 - ) , GPT ( P < 0. 05) ( P < 0. 05) 2. 2 GOT 22 GOT ( P < 0. 05) ( 2 - ) GOT 32,, 12, 48 ( P < 0. 05) ; ( P < 0. 05) ( 2 - ) ( P < 0. 05) ; 24 48, ACP ACP, ( P < 0. 05), ( P < ), Fig. 1 Effect of acute temperature stress on GPT activities in liver and serum of juvenile P. argenteus Different small letters above the bar graph indicate significant difference at different time ( P < 0. 05) ; capital letters indicate significant difference among experimental groups at the same time ( P < 0. 05). The same case in the following figures. Fig. 2 2 Effect of acute temperature stress on GOT activities in liver and serum of juvenile P. argenteus

4 Na + / K + -ATP, Fig. 3 Effect of acute temperature stress on AKP, ACP and ( P > 0. 05) ( 5 - ) ; 32 LDH activities in serum of juvenile P. argenteus,, 48, ( P < 0. 05) ( P <0. 05) ( 3 - ) 2. 5 LDH, ( P > 0. 05) ( 5 LDH 22,, 12 - ) ; 22,, 48, ( P <0. 05) ; 32 LDH ( P >0. 05) ( 3 - ) 2. 6 Na + 22 Na +,, 48 ( P < 12, 24, 0. 05) ; 32 Na + ( P < 0. 05), 24, ( P > 0. 05) , ( P < 0. 05) ( 6 - ( P <0. 05) ( 4 - ) 2. 7 K +, 48 ( P < 0. 05) ; 32, 24, 48, ( P < 0. 05) 12 2 ; ( P < 0. 05) ( 4 - ) 2. 8 Ca Ca , 12, 48, ( P < 0. 05) ( 4 - ) ; 32, 12, ( P < 0. 05) 12 ( P < 0. 05) 2. 9 Cl - 22 Cl -, 48 ( P < 0. 05) ; 32,,,, 24, 48, ( P < 0. 05) ( 4 - ) ( P < 0. 05) Na + /K + -ATP ( P < 0. 05) ;, 48 ( P < 0. 05) ( P < 0. 05) Na + / K + -ATP 32, 24, 48, ( P < 0. 05) Ca 2 + /Mg 2 + -ATP ) ; Ca 2 + / Mg 2 + -ATP 22 32, 48 ( P < 0. 05) 48 22, 22 K + ( P < 0. 05) Ca 2 + / Mg 2 + -ATP

5 2 : 63 4 Fig. 4 Effect of acute temperature stress on Na +, K +, Ca 2 + and Cl - contents in serum of juvenile P. argenteus Fig. 5 5 Na + / K + -ATP Effect of acute temperature stress on Na + / K + -ATPase activities in gill and kidney of juvenile P. argenteus Fig. 6 6 Ca 2 + / Mg 2 + -ATP Effect of acute temperature stress on Ca 2 + /Mg 2 + -ATPase activities in gill and kidney of juvenile P. argenteus , 24, 48 ( P <0. 05) ( 6-) ; [ 11-12] ( P <0. 05)

64 12 [ 21],, AKP [ 13] ACP,, [ 14-1 6] GPT GOT, [ 2 6-28] AKP, [ 29 ] GPT, GOT AKP [ 17],,,, [ 18] 2,, AKP,,, AKP ;,, GPT GOT, AKP,, 2,, AKP ACP [ 19] GPT,,, ;, ACP,, GPT, [ 2 4, 30 ], Na +,, ph,

6 64 12 [ 21],, AKP [ 13] ACP,, [ ] GPT GOT, [ ] AKP, [ 29 ] GPT, GOT AKP [ 17],,,, [ 18] 2,, AKP,,, AKP ;,, GPT GOT, AKP,, 2,, AKP ACP [ 19] GPT,,, ;, ACP,, GPT, [ 2 4, 30 ], Na +,, ph, GPT KUMAR [ 20] [ 21] [ 24],,, ;, GOT Cl - Na + K + [ 7 ],, Na + K + Cl - [ 3 1],, ( [ 22 ], K +, Na + Cl - [ 7 ] ) ; ;, 48 h, GOT ( Epinephelus septem-, KUMAR fasciatus), [ 20] [ 17 ],,, LDH GOT,,, [ 23 ] GOT LDH,, LDH,,,, Ca 2 +, [ 24 ],,,,,,, Ca 2 + LDH,, 4, GOT,, LDH,,,, AKP ACP 2,, ; K + [ 32 ] Ca 2 +, 4,,, 2 [ 3 3],,, ATP [ 25 ],,, ATP

2 : 65 ATP [ 34],, [ 35], ATP,,, [ 36 ] Na + /K + -ATP, Na + K +, [ 26 ] [ 37] Na + /K + -ATP, ; Ca 2+ / Mg 2 + - ATP 2 48, KONG [ 38], 2 ATP,,,,,,,,, : rameters and oxidative status of Japanese

7 2 : 65 ATP [ 34],, [ 35], ATP,,, [ 36 ] Na + /K + -ATP, Na + K +, [ 26 ] [ 37] Na + /K + -ATP, ; Ca 2+ / Mg ATP 2 48, KONG [ 38], 2 ATP,,,,,,,,, : rameters and oxidative status of Japanese flounder Paralichthys oli- [ 1],,,. vaceus[ J]. Aquaculture, 2014, 434 : [ J]., 2011, 35 ( 6) : [ 2],,,. 2012, 24 ( 3) : [ M]. :, 2003: [ 3],,,. [ J]., 2011, 23( 10 ) : [ J]., 2013, 37 ( 11) : [ 4],,,. ( Pampus argente- us) Na + / K + -ATP [ 20 ] KUMAR S, SAHU N P, PAL A K, et al. Short-term exposure to [ J]., 2013, 44 ( 5) : [ 5],,,. growth of fish Labeo rohita fed with high-protein diet[ J]. Aquac- [ J]., ult Nutr, 2013, 19( 2) : , 21( 4) : [ 6],,,. - HSP70 [ J]., 2011, [ J]., 2010, 34 ( 8) : [ 7],,,. [ J]., 2008, 20 ( 2 ) : [ J]., 2012, 36( 8) : [ 8] LAI C C, CROSBIE P B B, BATTAGLENE S C, et al. Effects of epitheliocystis on serum lysozyme activity and osmoregulation in cultured juvenile striped trumpeter, Latris lineata ( Forster) [ J]. Aquaculture, 2013, 388 / 389 / 390 /391( 2) : [ 9] DIETZ C, STILLER K T, GRIESE M, et al. Influence of salinity on energy metabolism in juvenile turbot, Psetta maxima ( L. ) [ J]. Aquacult Nutr, 2013, 19( s1 ) : [ 10],,,. ATP [ J]., 2011, 22 ( 4) : [ 11] MOREIRA I S, PERES H, COUTA A, et al. Temperature and dietary carbohydrate level effects on performance and metabolic utilisation of diets in European sea bass ( Dicentrarchus labrax) juveniles[ J]. Aquaculture, 2008, 274 ( 1) : [ 12],,,. GHIGF-IRNA /DNA [ J]., 2012, 8( 6 ) : [ 13] COUTO A, ENES P, PERES H, et al. Effect of water temperature and dietary starch on growth and metabolic utilization of diets in gilthead sea bream ( Sparus aurata) juveniles[ J]. Comp Biochem Phys A, 2008, 151( 1) : [ 14],,. [ J]., 2008, 38 ( 4) : [ 15] SONG Z D, LI H Y, WANG J Y, et al. Effects of fishmeal replacement with soy protein hydrolysates on growth performance, blood biochemistry, gastrointestinal digestion and muscle composition of juvenile starry flounder ( Platichthys stellatus) [ J]. Aquaculture, 2014, 426 /427 : [ 16] HAN Y Z, KOSHIO S, JIANG Z Q, et al. Interactive effects of dietary taurine and glutamine on growth performance, blood pa- [ 17],,,. [ J]., [ 18],,,. [ 19],,,. [ J]., 2014, 29 ( 6) : higher temperature triggers the metabolic enzyme activities and [ 21],,,. 31( 17) : [ 22],. 233.

66 12 [ 23],. [ J]. 4., 2000, 15( 1) : 13-14. [ 24],,,. [ J]., 2014, [ J]., 2013, 26( 5) : 16-20. [ 25],,,. al. Effects of salinity and temperature on the growth, survival, [ J].

8 66 12 [ 23],. [ J]. 4., 2000, 15( 1) : [ 24],,,. [ J]., 2014, [ J]., 2013, 26( 5) : [ 25],,,. al. Effects of salinity and temperature on the growth, survival, [ J]., 2007, 19 ( 5 ) : [ 26],,,. 314 ( 1 /2 /3 /4 ) : [ J]. [ 34],,,., 2008, 23( 1) : [ 27] REN M C, LIU B, HABTE-TSION H M, et al. Dietary phenylalanine requirement and tyrosine replacement value for phenylalanine of juvenile blunt snout bream, [ J]. Aquaculture, 2015, 442: Megalobrama amblycephala [ 28] YANG Q H, TAN B P, DONG X H, et al. Effects of different levels of Yucca schidigera extract on the growth and nonspecific immunity of Pacific white shrimp ( Litopenaeus vannamei) and on culture water quality[ J]. Aquaculture, 2015, 439: [ 29],,,. [ 37],,,. ( Cynoglossus [ J]., 2007, 16( 4) : [ 30],,, Na + / K + -ATP [ J]., [ 38 ] KONG X H, WANG G Z, LI S, et al. Effects of low temperature 2015, 11 ( 2) : [ 31],,,. muscle of mud crab ( Scylla paramamosain) [ J]. [ J]., 2009, 28 ( 1 ) : 1 - [ 32],,,. 33( 2) : [ 33] OSTROWSKI A D, WATANABE W O, MONTGOMERY F P, et whole body osmolality, and expression of Na + /K + -ATPase mrna in red porgy ( Pagrus pagrus) larvae [ J]. Aquaculture, 2011, Na + -K + -ATP [ J]., 2013, 35( 3 ) : [ 35],,,. Na + -K + -ATPase [ J]., 2014, 36( 3) : [ 36] LIN C H, TSAI R S, LEE T H. Expression and distribution of Na +, K + -ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge[ J]. Comp Biochem Phys A, 2004, 138( 3) : semilaevis) Na + -K + - ATPase [ J]., 2009, 40 ( 3 ) : acclimation on antioxidant defenses and ATPase activities in the 2012, 370 /371 : Aquaculture,

Microsoft Word - 22 栗志民.doc

Microsoft Word - 22 栗志民.doc 44 6 Vol.44, No.6 2013 11 OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2013 (Argopecten irradians concentricus) * 1 1 2 2 (1. 524025; 2. 524022) 2011 10 2012 4 (Argopecten irradians concentricus),,,,,,,, (P