2018 31 4 http / /www. psychjm. net. cn 621000 10 ICD - 10 110 34 38 38 60 CD3 + T CD3 + CD4 + T CD3 - CD56 + CD16 + NK P < 0. 05 CD4 + /CD8 + P < 0. 05 CD3 + CD8 + T P < 0. 05 CD3 + T CD3 + CD4 + T CD3 - CD56 + CD16 + NK CD3 + CD8 + T CD3 + T CD3 + CD4 + T CD3 + CD8 + T CD3 - CD56 + CD16 + NK R749. 4 A doi 10. 11886 /j. issn. 1007-3256. 2018. 04. 011 Immunophenotype analysis of lymphocyte subsets in patients with depression Ma YongnengHe FangZhang JieJiang WeiPeng BiQiu XiaoqinZeng Qinping The Third Hospital of Mianyang Sichuan Mental Health CenterMianyang 621000China Abstract Objective To explore the immunophenotyping of lymphocyte subsets in patients with depression and to compare the difference of the immunophenotyping among patients with depression at different levels of severityso as to provide reference for mechanismdiagnosis and treatment of depression. Methods 110 participants were diagnosed with depression according to International Classification of Diseases tenth edition ICD - 10 as patient groupand they were selected by cluster sampling. Among patient group34 patients were mild depression mild group 38 patients were moderate depression moderate group and 38 patients were severe depression severe group. During the same period60 healthy persons who had done physical examination in the Third Hospital of Mianyang were selected as the control group. Lymphocyte subsets were detected in all participants' blood samples by flow cytometry. Results The proportion and absolute value of CD3 + T lymphocytecd3 + CD4 + T lymphocyte and CD3 - CD56 + CD16 + NK cell in patient group were lower than those in control group P < 0. 05. Those data in the severe group were lower than those in mild and moderate groupand the data in moderate group were lower than those in mild group P < 0. 05. The CD4 + /CD8 + ratio showed the same results in all group P < 0. 05. The proportion and absolute value of CD3 + CD8 + T lymphocyte in patient group were higher than those in control groupand the data in the severe group were higher than those in mild and moderate groupthe moderate group were higher than those in mild group P < 0. 05. Conclusion The proportion and absolute value of CD3 + T lymphocytecd3 + CD4 + T lymphocytecd3 - CD56 + CD16 + NK cell of patients with depression are lower than healthy people while the proportion and absolute value of CD3 + CD8 + T cell are higher than healthy people. The proportion and absolute value of CD3 + T lymphocytecd3 + CD4 + T lymphocytecd3 + CD8 + T lymphocyte and CD3 - CD56 + CD16 + NK cell vary with different levels of severity of depression. Keywords Depression Lymphocyte subsets Flow cytometry Immune disorder 4 1 5 2 WHO 6 11% 3 7 2020 339
http / /www. psychjm. net. cn 2018 31 4 CD3 /CD16 + CD56 /CD45 /CD19 8 8920009 BNK 3 50 μl T BNK 9-10 5 s 15 min 4T BNK 450 μl 5 s 15 min 52 h 110 NovoExpress 1. 3 CD45 /SSC 1 1 1. 1 2017 12-2018 CD3 2 + CD8 + T 1 3 4 CD3 - CD19 + B CD3 10 International Classification of - CD56 + CD16 + NK Diseases tenth edition ICD - 10 4 2 Self - rating Depression Scale CD45 SSC CD45 /SSC CD45 + SSClow 1 2 CD3 + T 2 3 CD3 + CD4 + T SDS 53 3 4 1 2 3 110 60 1. 2 1 CD45 + SSClow 7 00-9 00 2. 0 ml TBNK CD3 + T CD3 + CD4 + T CD3 + CD8 + T CD3 - CD19 + B CD3 - CD56 + CD16 + NK ACEA NovoCyte D2040R 1 T BNK 220 μl CD3 /CD8 /CD45 /CD4 8920008 T 20 μl 340 2 CD3 + T
2018 31 4 http / /www. psychjm. net. cn 3 CD3 + CD4 + T CD3 + CD8 + T 4 CD3 - CD56 + CD16 + NK CD3 - CD19 + B 1. 4 2. 2 SPSS 16. 0 CD3 + T CD3 + CD4 + T CD3 - x ± s CD56 + CD16 + NK t CD3 + CD8 + T LSD - t P < 0. 05 P < 0. 05 CD3 - CD19 + B 2 2. 1 2. 3 53 57 20 ~ 55 33. 4 ± 11. 7 SDS CD3 + T CD3 + CD4 + T SDS 53 ~ 62 63 ~ 72 CD3 + CD8 + T CD3 - CD56 + CD16 + NK 73 34 38 38 30 30 20 ~ 55 34. 5 ± 12. 3 P > 0. 05 CD4 + /CD8 + P < 0. 05 1 2 P < 0. 05 CD3 + T CD3 + CD4 + T CD3 - CD56 + 20 ~ 35 36 ~ 55 CD16 + NK P < 0. 05 P > 0. 05 CD3 + CD8 + T 341
http / /www. psychjm. net. cn 2018 31 4 CD3 - CD19 + B P < 0. 05 P > 0. 05 3 4 1 CD4 + /CD8 + x ± s % CD3 + CD3 + CD4 + CD3 + CD8 + CD3 - CD19 + CD3 - CD56 + CD16 + CD4 + /CD8 + n = 110 63. 09 ± 5. 21 25. 65 ± 3. 28 32. 23 ± 5. 76 10. 27 ± 4. 25 18. 19 ± 3. 44 0. 79 ± 0. 25 n = 60 70. 73 ± 5. 07 43. 25 ± 4. 21 23. 27 ± 6. 45 10. 02 ± 2. 11 24. 25 ± 1. 61 1. 89 ± 0. 43 t 14. 57 17. 47 4. 68 23. 74 8. 75 26. 96 P < 0. 05 < 0. 05 < 0. 05 > 0. 05 < 0. 05 < 0. 05 2 x ± s CD3 + CD3 + CD4 + CD3 + CD8 + CD3 - CD19 + CD3 - CD56 + CD16 + n = 110 873 ± 65 413 ± 38 434 ± 38 409 ± 34 230 ± 26 n = 60 1414 ± 66 780 ± 65 419 ± 41 390 ± 23 418 ± 29 t 11. 23 12. 48 7. 62 21. 06 19. 54 P < 0. 05 < 0. 05 < 0. 05 > 0. 05 < 0. 05 3 CD4 + /CD8 + x ± s % CD3 + CD3 + CD4 + CD3 + CD8 + CD3 - CD19 + CD3 - CD56 + CD16 + CD4 + /CD8 + n = 34 67. 90 ± 3. 41 a 31. 05 ± 5. 31 a 27. 30 ± 3. 90 a 10. 52 ± 2. 25 21. 24 ± 2. 24 a 0. 94 ± 0. 21 a n = 38 63. 26 ± 6. 23 ab 23. 46 ± 4. 27 ab 32. 78 ± 6. 62 ab 9. 14 ± 3. 23 18. 90 ± 1. 22 ab 0. 79 ± 0. 23 ab n = 38 59. 24 ± 4. 22 14. 45 ± 5. 71 34. 23 ± 6. 29 10. 81 ± 4. 27 15. 65 ± 3. 01 0. 42 ± 0. 33 a P < 0. 05 b P < 0. 4 x ± s CD3 + CD3 + CD4 + CD3 + CD8 + CD3 - CD19 + CD3 - CD56 + CD16 + n = 34 906 ± 56 a 511 ± 67 a 406 ± 22 a 397 ± 27 547 ± 31 a n = 38 868 ± 75 ab 421 ± 55 ab 439 ± 36 ab 418 ± 31 452 ± 23 ab n = 38 853 ± 32 397 ± 72 456 ± 41 411 ± 26 314 ± 28 a P < 0. 05 b P < 0. 3 11 CD3 + CD4 + T CD3 + CD8 + T 14 - B 15 CD3 - CD56 + CD16 + NK 13 CD3 + CD4 + T NK CD3 + CD8 + T INF - γ TNF - β T 12 CD3 + CD4 + T CD3 + CD8 + T 342
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