Conclusions Expression of AGEs RAGE TGF-β1 are associated with preeclampsia. Key words Advanced glycation end product Transforming growth factor-β1 Ra

Size: px

Start display at page:

Download "Conclusions Expression of AGEs RAGE TGF-β1 are associated with preeclampsia. Key words Advanced glycation end product Transforming growth factor-β1 Ra"

匡娩孟
5 years ago
Views:

1 Prog Obstet Gynecol Jan.2017 Vol.26 No.01 AGEs RAGE TGF- β1 * 1a 1a** 1a 1a 1b 1c 2 1a 1. a. b. c AGEs RAGE -β1 TGF-β PE 6 AGEs RAGE TGF-β1 PE AGEs RAGE TGF-β1 P<0.05 TGF-β1 P<0.05 PE AGEs TGF-β1 PE TGF-β1 P<0.05 PE RAGE P <0.05 PE AGEs RAGE TGF-β1 AGEs-RAGE PE TGF-β1 -β1 R DOI /j.cnki.xdfckjz A Expression of AGEs RAGE TGF-β1 in hypertensive disorders complicating pregnancy. Su Min Zhang Yuquan Yao Wei et al. Department of Obstetrics and Gynecology the Affiliated Hospital of Nang tong University Nantong Abstract Objective To investigate the relationship between expression of advanced glycation end product AGEs its receptor RAGE transforming growth factor-β1 TGF-β1 and hypertensive disorders complicating pregnancy. Methods Samples from 900 pregnant women who presented to the Affiliated Hospital of Nangtong University for prenatal care from Aug.2014 to Oct.2015 were included in this prospective study of which 15 were subsequently developed hypertensive disorders complicating pregnancy as case group preeclampsia 6 and gestational hypertension 9 and 15 were normal pregnant women as control.the matemal serum was conducted in their second between 16 th to 20th week of pregnancy and third between 34th to 36th week of pregnancy trimester of pregnancy. Expression of serum AGEs RAGE TGF-β1 were detected by Elisa.Expression of AGEs RAGE TGF-β1 in the placentas were detected by immunohistochemical staining. Result In the second trimester of pregnancy the expression levels of AGEs RAGE TGF-β1 in the peripheral blood of pregnant women with preeclampsia were significantly higher than those in the control group P<0.05.The expression level of TGF-β1 in gestational hypertension was significantly higher than that in the control group P<0.05.In the third trimester of pregnancy the expression levels of AGEs and TGF-β1 in the peripheral blood of pregnant women with preeclampsia and gestational hypertension were both significantly higher than those in the control group P<0.05 while the expression level of Rage in preeclampsia was significantly higher than those in gestational hypertension and the normal control group. P<0.05.Positive expressions of AGEs RAGE TGF-β1 were detected in the placenta of preeclampsia.positive correlations were observed among AGEs RAGE TGF-β1. * ** No TDFzh2014 jsnt_zhangyuquan@ 163.com 15

2 Conclusions Expression of AGEs RAGE TGF-β1 are associated with preeclampsia. Key words Advanced glycation end product Transforming growth factor-β1 Rage Preeclampsia preeclampsia PE ml 2h 2500r /min 10min 1ml 1.5ml EP -80 PE 1.3 AGEs RAGE TGF-β1 ELISA PE AGEs RAGE TGF-β1 ELISA AGEs RAGE TGF-β1 PE RB 2 advanced glycation end products AGEs 1.4 RAGE AGEs RAGE TGF-β1 4μm 3%H 1 2 O 2 AGEs PE 10% AGEs RAGE TGFβ1 2h AGEs 30min DAB AGEs Fromowitz AGEs RAGE <5% 0 5% ~ 25% 1 26% ~ 50% 2 51% ~ 75% 3 75% β1 transforming growth factorβ1 TGF-β1 2 3 < SPSS16.0 x±s 珋 3 PE AGEs RAGEs pearson TGF-β1 r P< AGEs RAGE TGF-β1 PE AGEs RAGE TGF-β1 900 P<0.05 TGF-β PE 6 PE P<0.05 AGEs RAGE 22 P>0.05 AGEs RAGE TGF-β1 15 P < PE AGEs TGF-β1 <1 <1 P<0.05 PE TGFβ1 P < PE RAGE 1 P<0.05 BMI P > P>

2.2 AGEs RAGE TGF-β1 TGF-β1 AGEs RAGE PE AGEs r 1 = 0.382 P<0.05 TGF-β1 r 2 = 0.489 P<0.05 RAGE r 3 = 0.439 P<0.05 1 2.

3 2.2 AGEs RAGE TGF-β1 TGF-β1 AGEs RAGE PE AGEs r 1 = P<0.05 TGF-β1 r 2 = P<0.05 RAGE r 3 = P< AGEs RAGE TGF-β1 AGEs RAGE TGF-β1 TGF-β1 RAGE 23 Fisher's P < AGEs RAGE TGF-β1 2 AGEs RAGE TGF-β1 A D G PE B E H C F I A~ C AGEs D~ F RAGE G~ I TGF-β1 17

4 1 PE x±s 珋 n = 6 F n = 9 n = ± ± ± BMI Kg /m ± ± ± mmol /L 4.6± ± ± ± ± ± AGEs RAGE TGF-β1 x±s 珋 n AGEs ng /ml RAGE ng /ml TGF-β1 ng /ml ± ± ± ± ± ±0.7 * PE ±3.6 * 8.4±1.1 * 9.8±1.4 * ± ± ± ±2.9 * 6.2± ±0.9 * PE ±3.1 * 7.5±0.9 * 9.0±1.6 * * P<0.05 vs # P<0.05 vs 3 P RAGE AGEs PE AGEs RAGE TGF-β1 n n AGEs RAGE TGF-β PE NOS 3 NF-kB AGEs- AGEs RAGE AGEs NADPH NFκB TGF-β1 AGEs AGEs 4 RAGE AGEs 3 AGEs RAGE PE AGEs S100 / TGF-β1 Lyall 8 5 AGEs TGF-β1 mrna AGEs TGF-β1 AGEs RAGE TGF-β1 TGF-β1 PE PE RAGE PE PE TGF-β1 RAGE Oliver 6 PE mrna RAGE mrna TGF-β1 PE 9 srage RAGE TGF-β1 PE AGEs /RAGE TGF-β1 18 AGEs RAGE TGF-β1 PE RAGE TGF-β1 PE AGEs RAGE TGFβ1 AGEs-RAGE PE PE AGEs RAGE TGF-β1 AGEs RAGE TGF-β1 PE RAGE AGEs RAGE Konishi 7 AGEs 22

5 EMs Lafay Pillet MC Schneider A Borghese B et al.deep infiltrating endometriosis is associated with markedly lower body mass index a 476 case-control study J. Hum Reprod EMs 9 Al-Jefout M Palmer J Fraser IS. Simulaneous use of a levonorgestrel intrauterine system and an etonogestrel sudermal implant for debilitating adolescent endometriosis J.Aust N Z J Obstet Gynaecol J Brosens I Gordts S Benagiano G.Endometriosis in adolescents is ahidden progressive and severe disease that de- 11 Linda CG Johannes LHE David LH.Endometriosis in the serves attention not just compassion J. Hum Reprod Adolescent Pationt M.Endometriosis Science and Practice J 12 Elsheikh A Milingos S Kallipolitis G et al. Ovarian tu morsin young females a laparoscopic approach J.Eur J 3 Goldstein DP De cholnoky C Emansi SJ.Adolescent endometriosis J.Adolesc Health Care Sanfilippo JS Wakim NG Schikler KN et al.endometrio- 4 Janssen EB Rijkers AC Hoppenbrouwers K et al. Prevasis in association with uterine anomaly J. Am J Obstet Gynaecol Oncol lence of endometriosis diagnosed by laparoscopy in adolescents with dysmenor-rhea or chronic pelvic pain asystem- 14. Gynecol atic review J. Hum Reprod Update J M J J 櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠櫠 18 PE AGEs RAGE modulated in advanced glycation end products milieu via a RAGE receptor dependent pathway boosted by transfor- growth factor- beta 1 J.J Diabetes AGEs /RAGE ming PE TGF-β1 glycation end products in cellular signaling J.Redox Biol GDM J. PE RAGE Oliver EA Buhimschi CS Dulay AT et al. Activation of the receptor for advanced glycation end products system PE in women with severe preeclampsia J.J Clin Endocrinol Metab PE 7 Chekir C Nakatsuka M Noguchi S et al. Accumulation of advanced glycation end products in women with preeclampsia possible involvement of placental oxidative and nitrative stress J.Placenta Lyall F Simpson H Ni cola BJ et al.transforming growth PE factor-beta expression in human placenta and placental bed and fetal growth restriction J. Am J Pathol Alexander KL Mejia CA Jordan C et al. Differential re- 9 Martin E Ray PD Smeester L et al. Epigenetics and preeclampsia defining functional epimutations in the preceptor for advanced glycation end products expression in preeclamptic intrauterine growth restricted and gestational diabetic placentas J.Am J Reprod Immunol eclamptic placenta related to the TGF-beta pathway J. PLoS One 2015 e Yue S Zhou HM Zhu JJ et al. Hyperglycemia and Liver Ischemia Reperfusion Injury A Role for the Advanced Glycation Endproduct and Its Receptor Pathway J.Am J Transplant Serban AI Stanca L Geicu OI et al.extracellular matrix is 22 4 Christiane O Kathleen J Elisa H et al.role of advanced

综合报道

综合报道 55 doi:10.3969j.issn.1000-3606.2014.01.01 论著不同围生因素对妊娠的影响赵玉祥岳虹霓杨占华江苏省淮安市保健院新生儿科 ( 江苏淮安 223002) 摘要 : 目的探讨妊娠期高血压疾病胎膜早破等围生因素对妊娠的影响方法回顾性