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1 4 5 Vol. 4 No Journal of Food Safety and Quality Oct., 2013 * 张燕, 杨金易, 曾道平, 易娜, 孙远明 (, ) 摘要 :,,, 关键词 : ; ; ; Research progress on chemiluminescence immunoassay technique and its application in food safety detection ZHANG Yan, YANG Jin-Yi, ZENG Dao-Ping, YI Na, SUN Yuan-Ming * (College of Food Science, South China Agriculture University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Laboratory of Quality and Safety Risk Assessment in Agricultural Products Preservation Ministry of Agriculture, Guangzhou , China) ABSTRACT: Chemiluminescence immunoassay (CLIA) received more and more attention in recent years for its high sensitivity, wide linear range, simplicity, feasibility and non-isotopic contamination. The classification and principle of chemiluminescence immunoassay were introduced briefly in this article, and the CLIAs were classified systematically according to different marker substances of the chemiluminescence system and the difference of detection systems. A variety of methods were also introduced in detail. Moreover, its advantages and disadvantages were analyzed and the application of CLIA in food safety detection, including the detection of microorganism, toxins, pesticide residues, animal drug residues and the genetically modified food were discussed. The future development of CLIA was also prospected. KEY WORDS: chemiluminescence immunoassay; food safety; detection; application 基金项目 : ( ) (2010A , 2012A ) - ( ) (2012GA780001) Fund: Supported by National Natural Science Foundation of China ( ), the Science and Technology Project of Guangdong Province (2010A , 2012A ), the Science and Technology Project of Guangzhou City (Zhu Jiang Xin Xing) ( ), and the National Spark Program (2012GA780001) * 通讯作者 :,,, ymsun@scau.edu.cn *Corresponding author: SUN Yuan-Ming, Professor, College of Food Science, South China Agricultural University, Guangzhou , China. ymsun@scau.edu.cn

2 引言 (chemiluminescence, CL),,,,, 1977, Halman [1], (chemiluminescent immunoassay, CLIA) CLIA,,, CLIA [2-5], [6-8] CLIA, 2 CLIA 的类型及其原理 CLIA, [9],, (hν) ( ), CLIA, [10] 2.1 化学发光免疫分析,, [11], [12], N-, 430 nm HNO 3 +H 2 O 2 NaOH, Triton X-100, CTAC [13] 2.2 化学发光酶免疫分析 (chemiluminescent enzyme immunoassay, CLELA),,, (HRP) (ALP), 1,2- [14] HRP-CLEIA ( ), [15],,,,,,,, [16,17] (ALP) 1,2- [18] ALP-CLEIA Bronstein [19] ALP-AMPPD, AMPPD 1,2-, [17,20] CLEIA,

3 5, : 电化学发光免疫分析方法,, (electrochemiluminescence, ECL) (electrochemiluminescence immunoassay, ECLIA) ( ), (TPA), [21] Leland [22],,,, [23,24], [25] 3 在食品安全检测中的应用 3.1 食品中微生物的检测,,, [26] CLIA, ELISA 9 Gehring [27] O157:H7 CLEIA, ~ /ml, 400 CFU/mL, 6.5 h Magliulo [28] O157: H7 104~105 CFU/L, 90%~120% [29] CLIA, 3~100 CFU/100 ml, 0.994, 3~7, CLIA 3.2 食品中生物毒素的检测, CLIA, ng/kg, Fang [30] B ~10.00 ng/g, 0.01 ng/g, 12.2% 10.0%, 79.8%~115.4%,, , Lu [31] HRP-Luminol-H 2 O 2 B ng/g, 0.05 ~10.00 ng/g, 79.8%~115.4%, 15% ELISA, R 2 = Magliulo [32] M 1 CLEIA 1 pg/l, 1 ~200 pg/l; 96%~122%, 9% (HPLC), R 2 =0.98, CLEIA M 1,,, Quan [33] CLEIA B 1, 0.14~0.90 μg/l, 0.32 μg/l, 0.09 μg/l, ELISA 10 [34] B 1, IC ng/ml, 0.13 ng/ml,

4 %~115.4%, Yang [35] B(SEB) SEB 0.01 ng/ml, ELISA 1 ng/ml Luo [36] C 1 (SEC 1 ), 8.0~125.0 ng/ml, 0.5 ng/ml 92%~108% 92%~101%, ELISA Garber [37] 40 ECL ELISA, ECL (0.1~0.5 ng/ml) ELISA (0.5~10.0 ng/ml), ECL ELISA [38] A, IC pg/ml, 2.47 pg/ml, 66.97%~97.96%, ELISA R 2 = 食品中农药残留的检测,, Alexandra [39] DDT DDD, DDE DDT DDT μg/l, IC μg/l, 0.1~2.0 μg/l 0.07~1.00 μg/l, ELISA 4 Tudorache [40] 3 pg/l, IC pg/l, 10~1000 pg/l Waseem [41], 0.01~2 μg/l, 7.5 ng/ml, 97%~104% Chen [42] CLIA, 0.42 ng/l, 5~8000 ng/l, ELISA Jin [43], 0.04~5.00 ng/ml, ng/ml, 67%~122%,, (R 2 =0.8996) 3.4 食品中兽药残留的检测,,,,, :, [44] ;,, [45] Liu [46] -5-( ), 0.10~0.43 μg/l, 62.1%~110.3%, (R 2 =0.9379) Wu [47] pg/ml, 10~2000 pg/ml, 18%, 85%~105% Zhang [48] CLIA, HPLC r=0.93 Zhang [49] CLEIA, 6 ng/l, ELISA 10 Xu [50] CLEIA, 0.01 ng/ml, 0.03~23.70 ng/ml, 95%~123%, ELISA Cao [51] IC 50

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<4D F736F F D D D6ECD0A1BAEC2D2D2D2D2DB2CEBFBCCEC4CFD7D3A2CEC4B7ADD2EBB8F1CABDC3BBD3D0B6D4A1AD> 4 1 Vol. 4 No. 1 2013 2 Journal of Food Safety and Quality Feb., 2013 朱小红 *, 牟宵, 刘海静 (, 710061) 摘要 :,,, 关键词 : ; ; ; Standard formulation of the veterinary drug residues in catering service and its application