吕春茂, 等 : 平欧榛子油对高血脂大鼠的降脂作用 significantly increased 28%,32%,41% and 44%. Rats blood glucose (GLU),malonaldehyde (MDA),atherosclerosis index AI1,AI2 and

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1 LV Chunmao,et al: Effect of Flat -European Hybrid Hazelnut Oil on Hyperlipemia of Rats Induced by High Fat Diet Research Article 吕春茂, 陆长颖, 孟宪军, 董文轩, 魏雅静, 刘婵婵 ( ) : 采用 SD 大鼠建立高血脂症模型并灌胃处理, 通过测定模型血脂及肝脏病理变化, 考察平欧榛子油辅助降血脂和预防脂肪肝的作用结果表明 : 模型组的血脂水平与空白对照组差异极显著, 高血脂症模型建立 ; 大鼠灌胃结果显示, 饲喂豆油及平欧榛子油低剂量中剂量和高剂量大鼠总胆固醇 (TC) 的含量较高脂对照组大鼠差异显著, 分别下降 13% 13% 16% 和 16%; 甘油三酯 (TG) 的含量较高脂对照组大鼠差异也显著, 分别下降 24% 24% 50% 和 47%; 低密度脂蛋白 (LDL-C) 的含量较高脂对照组大鼠分别下降 1% 4% 6% 和 26%; 高密度脂蛋白 (HDL-C) 含量升高明显, 分别升高 28% 32% 41% 和 44%; 大鼠的血糖 (GLU) 丙二醛 (MDA) 动脉粥样硬化指数 AI1 AI2 和冠心指数 (R-CHR) 均显著降低, 并与榛子油的剂量呈现正相关 ; 连续饲喂平欧榛子油有助于降低高血脂症大鼠肝脏脂质沉积, 对大鼠肝细胞有一定的保护作用 : 平欧榛子油 ;SD 大鼠 ; 高血脂 ; 脂肪肝 :TS :A : (2014) Effect of Flat-European Hybrid Hazelnut Oil on Hyperlipemia of Rats Induced by High Fat Diet LV Chunmao, LU Changying, MENG Xianjun, DONG Wenxuan, WEI Yajing, LIU Chanchan (College of Food Science,Shenyang Agricultural University,Shenyang ,China) Abstract: The hyperlipidemia model in SD rats was established and rats stomached in this manuscript. Through the determination of blood lipid and liver pathological changes in model rats, effect of Flat-European hybrid hazelnut oil on reducing blood lipid and preventing fatty liver were examined. The results showed that the levels of lipid between the model group and the control group were significantly different. The hyperlipidemia model was established. The rats gastric lavage results showed that rats fed soybean oil and Flat-European hybrid hazelnut oil low doses,middle doses and high doses total cholesterol(tc) content were significantly decreased by 13%,13%,16% and 16% compared to the high fat control group. Compared to the high fat control group rats triglyceride (TG) content were obviously different and reduced to 24%,24%,50% and 47%, respectively. Rats low density lipoprotein (LDL-C) content compared to the high fat control group were decreased by 1%,4%,6% and 26%. The rats high density lipoprotein (HDL-C) content were : : (F ) : (1970 ),, bt_lcm@126.com 330 Journal of Food Science and Biotechnology Vol.33 No

2 吕春茂, 等 : 平欧榛子油对高血脂大鼠的降脂作用 significantly increased 28%,32%,41% and 44%. Rats blood glucose (GLU),malonaldehyde (MDA),atherosclerosis index AI1,AI2 and coronary index (R-CHR) were significantly decreased and showed a positive correlation with hazelnut oil dose. Continuous feeding Flat-European hybrid hazelnut oil helped to reduce lipid deposition in liver in hyperlipidemia rats,had certain protective effect on rat liver cells. Keywords: Flat-European hybrid hazelnut oil,sprague-dawley rats,hyperlipemia,fatty liver (total cholesterol,tc) (triglyceride,tg) [1] 1 ( ) 1.1 SD(sprague-dawley) 60~80 g ( ) [2-3], SPF ( [4] :SYXK( ) ) 20~26 40%~70% 12~ [5] 24 h 1.2, [6] 20 ; ( [7] 60.5% [8] ): 7.2% 2.5% 2.3% 24.2% 6.9%) ; 77.7%, ; 9.5% [9] [15], ; :78.8%,1%,10%,10%,0.2% ; ; ; [10-11], ; [12] [13-14], ;, ; (MDA) (TC) (TG) (GLU) 1.3 (HDL-C) (LDL-C) (MDA) ;TDL-5000B, ;HH-6 ;DHG-9070A ;ST

3 LV Chunmao,et al: Effect of Flat -European Hybrid Hazelnut Oil on Hyperlipemia of Rats Induced by High Fat Diet Research Article ;KQ-300DE 12 h 3 ml/kg,, 30 min,3 000 r/min ;XS min 榛子油的制备 20 (HDL-C) (LDL-C) 200 g (MDA) TBA : (g/ml), 70% AI1=(TC-HDL-C)/HDL-C, (210 W) min r/min 20 min 肝脏病理切片的制作 [16], 4 mm 3, 模型构建与处理 60 SD, d, 1.0 ml,, (TC) (TG), 10, 统计学处理 (500 mg/(kg d) ) (500 mg/(kg d)) ± (x 軃 ±s) (50 mg/(kg d)) (500 mg/ t p>0.05 (kg d)) (1 000 mg/(kg d)) ;p<0.05, ;p< (500 mg/(kg d) ) d 指标测定,20 d 40 d 1.0 ml,3 000 r/min 15 min Table 1 1 (TC) (TG) /(mmol/l) /(mmol/l) (TC) (TG) (GLU), AI 2 =LDL-C/HDL-C R-CHR=TC/HDL-C - (H.E) SPSS17.0 ANOVA Effects of hazelnut oil on blood lipid parameters in rats (HDL-C) /(mmol/l) (LDL- (GLU) C) /(mmol/l) /(mmol/l) (MDA) /(nmol/l) ±0.17 a 0.19±0.05 a 0.97±0.13 a 0.25±0.06 a 7.42±1.06 a 4.23±0.31 a ±0.23 b 0.58±0.04 b 0.78±0.08 b 1.05±0.11 b 12.34±1.17 b 9.70±0.78 b ±0.20 c 0.44±0.06 c 1.00±0.14 c 1.04±0.09 b 11.88±1.23 c 7.76±0.59 c ±0.39 c 0.44±0.07 c 1.03±0.16 c 1.01±0.08 b 11.14±1.16 d 6.64±0.42 d ±0.24 c 0.29±0.05 d 1.10±0.13 d 0.99±0.12 b 10.70±0.85 e 6.12±0.43 e ±0.21 c 0.31±0.06 d 1.12±0.15 d 0.78±0.05 c 9.38±1.28 f 5.87±0.39 f 榛子油对总胆固醇的影响 1 13% 13% 16% 16% 3 TC (p< TC, 0.01), TC TC, 332 Journal of Food Science and Biotechnology Vol.33 No

4 吕春茂, 等 : 平欧榛子油对高血脂大鼠的降脂作用榛子油对甘油三酯的影响 1 TG (p< 榛子油对血糖的影响 ), GLU (p<0.01), TG, 24% 24% 50% 47% 3 GLU, 4% 10% 13% TG, 24% 3 GLU TG GLU 榛子油对高密度脂蛋白胆固醇的影响 1, HDL-C 榛子油对脂质过氧化代谢物丙二醛的影响 (p<0.01), 1, MDA (p<0.01) HDL-C, 28% 32% 41% 44% MDA, 3 20% 32% 37% 39% 3 HDL-C MDA, HDL-C, MDA 榛子油对低密度脂蛋白胆固醇的影响 LDL-C 1, LDL-C R-CHR 2.2 AI 1 AI 2 (p<0.01), TC HDL-C LDL-C, AI 1 =(TC- LDL-C HDL-C)/HDL-C AI 2 =LDL-C/HDL-C 1% 4% 6% 26% 3 R-CHR=TC/HDL-C 2,AI 1 LDL-C AI 2 R-CHR AI 1 AI 2 R-CHR Table 2 2 Effects of hazelnut oil on blood lipid index in rats AI 1 AI 2 R-CHR ±0.03 a 0.26±0.02 a 1.27±0.27 a ±0.19 b 1.35±0.14 b 2.81±0.29 b ±0.04 c 1.04±0.06 c 1.90±0.14 c ±0.14 c 0.98±0.05 c 1.84±0.24 c ±0.08 d 0.90±0.09 c 1.67±0.18 d ±0.04 d 0.70±0.03 d 1.64±0.14 d

5 LV Chunmao,et al: Effect of Flat -European Hybrid Hazelnut Oil on Hyperlipemia of Rats Induced by High Fat Diet Research Article 2.3,, [18], ; ;, [17] H.E 1,, ;,,,, Fig. 1 1 Slice of rat liver of different treatment during feeding period 3, 70 d TC TG, 40 d (50 mg/(kg d)) (500 mg/(kg d)) (1 000 mg/(kg d)) TC TG LDL-C GLU MDA, AI 1 AI 2 R-CHR HDL-C [19] 334 Journal of Food Science and Biotechnology Vol.33 No

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中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗 1338 中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用陈菲 1 2,, 艾国 3, 盛柳青 1, 麻佳蕾 1, 黄正明 1. 金华职业技术学院医学院, 浙江金华