SD STZ60mg/kg 3 >11.1mmol/L g/kg d SD , 2.25g/kg d 22 : 2: /3 1/4 22 INS MDA 21 CAs 45

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戎居茅
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2 SD STZ60mg/kg 3 >11.1mmol/L g/kg d SD , 2.25g/kg d 22 : 2: /3 1/4 22 INS MDA 21 CAs 45

3 60 W256 2 IgG IgM TNF g/kg d g/kg d DA NE >30%

4 Preliminary Study On Eugenicpharmacological Effects of Liuwei Dihuang Pill Postgraduate Wei, Han Advisor Associate Professor Jiang, Wenyue Department Department of Integrated Traditional Chinese & Western Medicine Abstract Aim To study the effects of Liuwei Dihuang Pill (LDP) on the offsprings of gestational hyperglycemia and hot constitution rats. Contents 1. To study the effects of LDP on pregnant rats with severe hyperglycemia and their offsprings. 2. To study the effects of LDP on hot constitutions rats and their offsprings. Methods 1. To investigate effects of LDP on pregnant rats with severe hyperglycemia and offspring. SD female rats were induced hyperglycemia by an i.p. injection of STZ (60mg/kg), and fasting PG levels were tested 3 days later, PG>11.1mmol/L were chosen as model, 1w after the injection, the rats were mated with the male. The morning that conception was verified by the presence of sperm in a vaginal smear was designated gestational day 0. On gestational day 19, maternal blood was taken from abdominal aorta and fetuses were separated by c-section and observed. On gestational day 3, half of models were treated with 2.25g/kg d LDP. 2. To study the effects of LDP on hot constitutions rats and offspring. According to the anus temperature in 6 consequent days female rats were separated into two groups: cold and hot constitution. The male were also distinguish as the same way: lower and higher temperature. Fasting PG was tested among females. In the following 22 days, 10 hot female rats were medicated with 2.25g/kg d LDP, other 2

5 female rats with normal feeds as control group. Then anus temperature, fasting PG and urine catecholamines(cas) was determined. Two female mated with one corresponding male. Parturition progress was monitored and then each of the pup was weighed. 1/3 1/4 newborn rats from each dam were killed at 22h after delivery, and their PG, INS, MDA levels were analyzed. 21days after delivery part of the other pups PG and urine CAS were measured. Some other pups anus temperature, PG and INS levels were measured 45 days after weaning. Walker 256 (W256) carcinosarcoma cells were implanted subcutaneously 60 days after delivery. Two weeks later, rats were killed to test IgG, IgM, TNF-α and weigh Spleen, Thymus. Results 1. Compared with control group, the plasma glucose, body weight, body length, tail length, and tail length in fetuses from dams with severe hyperglycemia decreased markedly, while the insulin content in pancreas and placenta weight increased. LDP at the dose of 2.25g/kg d could modulate the above parameters to varying degrees. 2. Compared with hot constitution control, LDP at dose of 2.25g/kg d could increase the weight of hot constitution newborn rats. With aging, the weight of pups from the medicated dams was lower than that of offspring from hot constitution control, who showed catch-up tendency; significantly decreased PG in newborn rats, but with aging, statistic difference disappeared; significantly decreased INS in newborn rats, and had no statistic difference with the cold constitution ones, at some degree increased INS of hot constitution pups; significantly decreased DA and NE of pups, had no statistic difference with the cold constitution ones, and decreased E of pups; decreased temperature to some degree; significantly decreased MDA in newborn rats; decreased carcinoma weight to some degree, the rates of anti-carcinoma were above of 30%. Conclusions 1. LDP could modulate the decreased level of weight, body length, tail length, PG and increased level of INS, placenta weight in fetus led by severe maternal hyperglycemia. 2. LDP could modulate the decreased level of birth weight, INS and increased level of PG, MDA, temperature and urine catecholamines in F1 led by hot constitution. 3. LDP could inhibit the F1 carcinosarcoma growth in maternal hot constitution. Key words LDP; maternal hot constitution and cold constitution; maternal hyperglycemia; blood glucose; offspring 3

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10 8 1. SD g SYXK( ) STZ Sigma PH M W HCL NAOH EDTA

11 E Sigma 2 8 DA Sigma NE H ml 250ml 15ml ph5.4 EDTA3.74g 1mol/L 100ml ph8.2 5mol/L NAOH ph mg 5g 100ml 25 55mol/L NAOH 1:9 38 Anton g 1000ml 2mol/L HCL500ml 90 45min 2mol/L HCL250ml 70 10min 2mol/L HCL500ml 50 45min 200ml/ ph h 200 2h MC 3 2 CARY Eclipse LG-R-80 ZK380 BHG LD4 2 BP211D 0.1mg ph CHM

12 1. 1 SD STZ60mg/kg 12 3 >11.1mmol/L 40 2 STZ 7 17:00 8: , /60kg d g 2.25g/kg d SD i.p. 7 i.p.stz60mg/kg CT PG>11.1mmol/L PG,ING,LP, PG,ING 1 LDP Fig 1 Flow chart of studying effects of LDP on pregnant rats with severe hyperglycemia 10

13 mg/kg SD 6 x x -1 x SD 2.25g/kg d 22 2 LDP Fig 2 Flow chart of studying effects of LDP on F0 rats of hot constitution 11

14 (3) : 2:1 5 8: W256 IgG IgM TNF- 3 LDP Fig 3 Flow chart of studying effects of LDP on F1 rats of hot constitution 6 1/3 1/

15 2 IgG IgM TNF W256 Wistar ml/ 60min IGG IGM TNF-, SI TI CAs E NE DA CAs CAs CAs 24 CAs mol/LHCL PH gEDTA 5mol/L NAOH PH 8.5 1g rpm 5 0.1mol/L 4ml 10 1ml 0.25mol/L1ml EDTA0.2ml PH Y 1.2ml PH ml ml 2.5 5mol/L 0.5ml 2 13

16 A nm NE nm.NE 10, nm DA E NE300ng DA500ng 0.01mol/LHCL 200 g/ml 48, r/min 15min 4 INS 50 6ml 4 600r/min 30min 3000r/min 15min 5 MDA, 4 10% TBA 51 6 IgG 3000r/min 15min 7 IgM 3000r/min 15min 8 TNF- 3000r/min 15min 9 a10 a150 10S S -1 1ml 10-20IU/ml 4. SPSS11.5, T Person 14

- 14 - Drug Evaluation Research 第 36 卷第 1 期 2013 年 2 月料 ), 山西皇城相府药业有限公司, 批号 20111003 ( 人口服推荐剂量为每日 3 次, 每次 4 粒, 成人体质量按 60

- 14 - Drug Evaluation Research 第 36 卷第 1 期 2013 年 2 月料 ), 山西皇城相府药业有限公司, 批号 20111003 ( 人口服推荐剂量为每日 3 次, 每次 4 粒, 成人体质量按 60 Drug Evaluation Research 第 36 卷第 1 期 2013 年 2 月 - 13 - 柴青消癖胶囊对乳腺增生模型大鼠的治疗作用研究 * 刘聪, 岳永花, 郝旭亮山西省中医药研究院, 山西太原 030012 摘要 : 目的探讨柴青消癖胶囊对乳腺增生模型大鼠的治