Microsoft Word 肖新峰-二校出4147、4150、4151、4152

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1 6 10 Vol. 6 No Journal of Food Safety and Quality Oct., 2015 肖新峰 1, 赵丽华 2, 张慧敏 1, 赵清波 1, 吴文惠 1, 包斌 (1., ; 2., ) 1* 摘要 : 目的 (Allium Mongolicum Regel) 方法 ( ) 6 wk, wk, 2 wk,, (atherosclerosis index, AI) (lipid comprehensive index, LCI) 结果 ; wk 2,, (total cholesterol, TC) (triglyceride, TG) (low density lipoprotein cholesterol, LDL-C) AI LCI wk 6,, TC TG LDL-C AI LCI 36.25% 58.79% 37.78% 54.98% 78.61% 结论 TC TG LDL-C, AI LCI,,, 关键词 : ; ; ; Inhibitory effect of ethanol extracts from Allium Mongolicum Regel on hyperlipidemia mice XIAO Xin-Feng 1, ZHAO Li-Hua 2, ZHANG Hui-Min 1, ZHAO Qing-Bo 1, WU Wen-Hui 1, BAO Bin 1* (1. College of Food Science & Technology, Shanghai Ocean University, Shanghai , China; 2. College of food & Technology, Inner Mongolia Agricultural University, Hohhot , China) ABSTRACT: Objective To investigate the lipid-decreasing and inhibitory effect of ethanol extracts from Allium Mongolicum Regel (AMR) in mice. Methods The hyperlipidemia mice were induced by high fat diet. The female Kunming mice were randomly divided into control group, blank group, and intervention groups (low, middle and high dose intervention groups). The experiment was lasted for 6 wk. Weighing body mass of mice every week, and the total cholesterol levels (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in plasma were measured every 2 wk. Atherosclerosis index (AI) and lipid comprehensive index (LCI) were calculated. Results The high-fat model was established by the high fat diet. Starting from the wk 2, the level of TC, TG, and LDL-C, and the value of AI and LCI in plasma could be decreased in the hyperlipidemia mice by AMR extracts with meddle and high 基金项目 : ( ) Fund: Supported by the National Natural Science Foundation of China ( ) * 通讯作者 :,, bbao@shou.edu.cn *Corresponding author: BAO Bin, Associate Professor, Shanghai Ocean University, No.999, Hucheng Ring Road, Pudong New District, Shanghai , China. bbao@shou.edu.cn

2 dose. In the wk 6, the decrease of TC, TG, and LDL-C level and AI and LCI value in high-dose intervention groups reached to 36.25%, 58.79%, 37.78%, 54.98% and 78.61%, respectively, compared with those in blank group. Conclusion AMR extracts have an obvious effect on lowering blood lipid of hyperlipidemia mice, potentially preventing and reducing the incidence of atherosclerosis. KEY WORDS: Allium Mongolicum Regel; hyperlipidemia; atherosclerosis index; lipid comprehensive index 1 引言, [1], (total cholesterol, TC) (triglyceride, TG),,, [2] (Allium mongolicum Regel),,, ( ) ( ) ( ) ( ) ( ) [3] :,,,,,,, [4],,,, Kumari [5] ; Chen [6], TG, [7-9],, 2 材料与方法 2.1 材料与仪器沙葱 , 48 h 实验动物 SPF ( : SCXK ), 18~22 g, 实验仪器 (SAII-2, ); (Avanti J-26XP, ); (R206D, ); (JYL-C501, ); (AU5800, ) 2.2 实验方法 [9] 沙葱提取物的提取, 100 g 150 ml 95%,,, r/min 20 min, ( 60, ), 10 g( )/ ml, 小鼠饲料的制备 : [10] 78.8%, 1%, 0.2%, 10%, 10%, ( ) : 2.5 ml 100 g, 0.25 g, ; : 7.5 ml 100 g,

3 10, : g, ; : 15 ml 100 g, 1.5 g, 实验动物分组及喂食 :, 20~24, 12 h:12 h - 128, 1 wk, 5, ( 0.1 ml ) ( 0.3 ml ) ( 0.6 ml ),, : ( ) 6 wk, 32, wk 1 8, 2 wk 8 ; 6 wk, 24, 2 wk 血浆中血脂水平的测定及血脂综合指数动脉粥样硬化指数的计算, 12 h,, 2 h, 4 10 min,, (TC) (TG) (high density lipoprotein cholesterol, LDL-C) (low density lipoprotein cholesterol, LDL-C), (atherosclerosis index, AI) (lipid comprehensive index, LCI) : TC TG LDL C LCI= HDL C TC HDL AI= HDL C 脏器指数,,,,, 统计方法 SPSS19.0, ± ( X S ) 3 结果与讨论 3.1 沙葱提取物对小鼠体重的影响,,, ( 1) 1, wk 3 wk 6, wk 5, [11], 3,, 3,,, Table 1 表 1 沙葱提取物对小鼠体重的影响 (n=8) Effect of AMR extracts on growth indicators of mice (n=8) (g) wk 1 wk 2 wk 3 wk 4 wk 5 wk ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±4.85 * 38.08± ± ± ± ± ± ± ±3.72 * 37.19±2.88 *

4 沙葱提取物对小鼠脏器指数的影响,, [10] ( 2) 2, wk 2 wk 4 wk 6, 3,,,,,,, 3, wk 2, [12], [13],, 3.3 沙葱提取物对小鼠血脂指标的影响 TC TG LDL-C HDL-C,,, 5 wk 2 wk 4 wk 6, 8, TC TG HDL-C LDL-C ( 1) 1,, TC TG HDL-C LDL-C,, TC TG HDL-C LDL-C wk 2, TC, 3,, TC wk 4, wk %, wk %, TC TC,, Table 2 表 2 沙葱提取物对小鼠脏器指数的影响 (n=8) Effect of AMR extracts on visceral organ of mice (n=8) wk 2 wk 4 wk ± ± ± ± ± ± ±0.37 * 5.86± ± ±0.36 * 5.44± ±0.37 * 6.28± ± ±0.31 * 0.28± ± ± ± ± ± ±0.04 * 0.48± ± ± ± ± ±0.08 * 0.48± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.18

5 10, : (n=8) Fig. 1 Effect of AMR extracts on blood lipid indicators of mice (n=8) TC, TG TC, wk 2 ; wk 6 3, 25.45% 33.33% 58.79%,, TC HDL-C,, HDL-C, LDL-C, ;, wk 4 LDL-C, wk 6 ; wk 2 LDL-C, wk 4 wk 6 ; LDL-C 3.4 沙葱提取物对小鼠动脉粥样硬化指数和血脂综合指数的影响,, (AI) (LCI) TC TG LDL-C HDL-C4, [14] 2,, AI LCI, AI, wk 2, wk 6 2, 36.08% 54.98% LCI, wk 2 ; wk 6, 32.24% 67.30% 78.61%, wk 4 wk 6,,,

6 (n=8) Fig. 2 Effect of AMR extracts on AI and LCI indicators of mice (n=8) [15], 5 mg/g,, Song [15] TC TG LDL-C, HDL-C 4 结论, TC TG LDL-C, AI LCI,,,, 参考文献 [1],,. [J]., 2010, 26(5): Zhai R, Lv LS, Jin BQ. Hypolipidemic effect of the polysaccharide from polygonum multiflorum [J]. Food Mach, 2010, 26(5): [2],. [J]., 2005, 21(1): Zhou GH, Yu GP. Effect study of auricularia polysaccharide on reducing blood lipid [J]. Mod Food Sci Technol, 2005, 21(1): [3],,,. [J]., 2007, 26(7): He FY, Liu SZ, Yan ZZ, et al. The resources distribution protection and utilization of wild Allium mongolicum Regel [J]. Chin Wild Plant Res, 2007, 26(7): [4] Sakka RL, Boughelleb MN, El AA, et al. Phytochemicals, antioxidant and antifungal activities of Allium roseum var. grandiflorum subvar. typicum Regel [J]. S Afr J Bot, 2014, 91(0): [5] Kumari K, Augusti KT. Lipid lowering effect of S-methyl cysteine sulfoxide from Allium cepa Linn in high cholesterol diet fed rats [J]. J Ethnopharmacol, 2007, 109(3): [6] Chen YC, Kao TH, Tseng CY, et al. Methanolic extract of black garlic ameliorates diet-induced obesity via regulating adipogenesis, adipokine biosynthesis, and lipolysis [J]. J Funct Food, 2014, 9(0): [7] Li YL, Luo RM. Antibacterial effect of extracts from Allium mongolicum Regel [J]. J Anhui Agric Sci Technol, 2008, 9(4): [8],,,. [J]., 2013, 35(1): Wang JK, Yang F, Zhao LH, et al. Analysis and comparison of nutritional components in Allium mongolicum Regel and chinese chive (Allium tuberosum Rottler) [J]. Acta Nutr Sin, 2013, 35(1):

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The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the american gastroenterological association, american association for the study of liver diseases, and

7 10, : 4153 [9],,,. [J]., 2014, 35(11): Ma CJ, Zhao LH, Yang F, et al. A contrast research of bacteriostatic effect by three common liliaceous plants [J]. Sci Technol Food Ind, 2014, 35(11): [10] Luo J, Quan J, Tsai J, et al. Nongenetic mouse models of noninsulin-dependent diabetes mellitus [J]. Metabolism, 1998, 47(6): [11],,,. [J]., 2013, 13(9): 1 7. Ji XF, Xu HD, Liu YC, et al. Antihyperlipidemic and antioxidant effects of flavonoids and polysaccharides from Chaenomeles Sinensis (Thouin) koehne [J]. J Chin Inst Food Sci Technol, 2013, 13(9): 1 7. [12] Chalasani N, Younossi Z, Lavine J E, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the american gastroenterological association, american association for the study of liver diseases, and american college of gastroenterology [J]. Gastroenterology, 2012, 142(7): [13]. Hohhot: Inner Mongolia Agricultural University, [14],,,. [J]., 2013, 34(10): Zhang CX, Qi YG, Cao B, et al. Effect of micro-milling on reducing blood lipid of Fructus Crataegi insoluble dietary fiber [J]. Sci Technol Food Ind, 2013, 34(10): [15]. [D]. :, Sa RL. Studies on extraction process, structural identification and related biological activity of Allium Mongolicum Regel flaonoids [D]. Hohhot: Inner Mongolia Agricultural University, 2014 [16] Song L, Dong L, Bo H.et al. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves [J]. J Ethnopharmacol, 2013, 149(1): 作者简介 ( 责任编辑 : 金延秋 ) 肖新峰, 硕士研究生, 主要研究方向为食品品质与营养评价 xiaoxinfeng123@126.com [D]. :, Zhao CY. Studies on Separation, Purification and structural characterizations of flavonoids from Allium Mongolicum Regel 包斌, 副教授, 主要研究方向为食品营养研究 bbao@shou.edu.cn and its Effects on immunity and antioxidant function in mice [D].

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗 1338 中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用陈菲 1 2,, 艾国 3, 盛柳青 1, 麻佳蕾 1, 黄正明 1. 金华职业技术学院医学院, 浙江金华

Microsoft Word 肖新峰-二校 出4147、4150、4151、4152

Microsoft Word 肖新峰-二校出4147、4150、4151、4152