Microsoft Word - 17 韩涛.doc

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1 44 6 Vol.44, No OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2013 (Portunus trituberculatus) * 1, ( ; ), 20g (Portunus trituberculatus), 0.003% % 0.655% 0.986% 1.303% 1.628% 8,, 0.003% 1.628% (P>0.05),, 0.655%,, 0.6%,, 0.6% ; ; ; S963 (Teshima, 1997; Hernández et al, 2004; Holme et al, 2006),,,,, VD(Akiyama et al, 1992; Sheen et al, 1994),, (Teshima et al, 1971) (Harrison, 1990) (Conklin et al, 1980), (Hernández et al, 2004) (Irvin et al, 2010) (Briggs et al, 1988) (Chen, 1993)(Gong et al, 2000) (Thongrod et al, 1998) (Kean et al, 1985) (Sheen, 2000) (Holme et al, 2006) (Portunus trituberculatus) (, 2010), (, 2010) (, 2011),, (Portunus trituberculatus), *, Y ;, 2012C ;, 2010R ;, 2013GA701041, 2013GA ;, 2013C32037, 2013C31032 ;, 2011 ;, 2013C41007 ;, 2011B81003 ;, 2013C11017 ;,,, goodhantao@gmail.com :,, wangchunlin@nbu.edu.cn : , :

2 6 : (Portunus trituberculatus) , 6, CH1 CH6 1, (2 : 1), 60, 15min,,,,, 50 24h , 2, ( 30cm, 40cm),, 20g, 18, (6:00 18:00), 8%, 2,, 15%, 26 29, >7mg/L, ph , 26 29, 1.3, 24h, 12 75,, 2, (Weight gain)(specific growth rate)(feed conversion ratio) (Survival)(Hepatopancreas index) (Gland index) (Carapace length gain) (Carapace width gain) (Carapace height gain) : WG(%) = 100 ( )/ ; SR(%) = 100 /; SGR(%) = 100 (ln ln )/; (FCR) = /( ); (GSI) = 100 /; (HI) = 100/; (CLG) = 100 ( )/ ; (CWG) = 100 ( )/; (CHG) = 100 ( )/ ;, 105 ; (K-355/K438, BUCHI, Swiss), (E-816, BUCHI, Swiss), ; 550 (CHO) 1.5, SPSS 11.5(SPSS, IL, USA),, Duncan s, P< , 83% 100%, (P>0.05), CH3, CH6, CH1 CH5, CH3, CH5, CH3 CH5, CH5, CH2, CH1 CH3 CH5, 1, 0.6%, Y = X Y = X , 0.6%,

3 表 1 实验饲料配方组成 Tab.1 Composition of experimental diets ) ) % (%) ) (%): B 1 0.5; 0.8; 2.6; 0.1; 1.5; B 6 0.3; 0.5; 18.1; C 12.1; K 3 0.2; 3.0; B ; A 2.5; D 3 2.5; E 5; ) (g/kg mixture): ; ; 42.03; ; 10.78; 38.26; 44.19; 4.74; 0.33; 0.22; 0.43; 0.02; 32.33; ,, (P<0.05) CH5, CH2 CH3 CH4 CH5, CH3, CH3, CH6, (P>0.05) 4, (P<0.05), CH5, CH2 CH6, CH1 CH4 (P>0.05) CH5, CH1 (P> 0.05) 3, 80% 83.33% 100%, 0.655%,,, (Ishikawa et al, 1997),, (Lovell, 1989),, 0.655%,, 1.628% (Hern ndez et al, 2004) (Holme et al, 2006) 表 2 饲料不同胆固醇添加量对三疣梭子蟹幼蟹生长和利用的影响 Tab.2 Response of swimming crab juveniles to various levels of cholesterol supplementation on growth performance and feed utilization (g) (g) (%) ab ab b ab ab a (SGR) ab ab a ab b ab (%) (GSI) a ab b ab b ab (HI) ab a ab ab b ab (n=3), (P<0.05)

4 6 : (Portunus trituberculatus) Fig.1 Relationship between weight gain and dietary cholesterol level in juvenile swimming crab (Sheen, 2000), (Mercer, 1982), 0.655%, (Ishikawa et al, 1997), 0.12% 2%(Teshima, 1985, 1991) 2.6 6g/kg() ( 3% 7%)(Ishikawa et al, 1997) 0.11% 0.51%(Sheen, 2000) 0.8% 74.3%, % 11 (Holme et al, 2006),, 4.0g/kg( ) (Irvin et al, 2010) Tab.3 表 3 饲料不同胆固醇添加量对三疣梭子蟹幼蟹壳宽壳长和壳高的影响 Response of swimming crab juveniles to various levels of cholesterol supplementation on carapace width, carapace length and carapace height ICW(cm) FCW(cm) CWG(%) ab b b b a ab ICL(cm) FCL(cm) CLG(%) ab ab b ab a ab ICH(cm) FCH(cm) CHG(%) ab ab b ab ab a (n=3), (P<0.05) 表 4 饲料不同胆固醇添加量对三疣梭子蟹幼蟹体成分的影响 (%) Tab.4 Response of swimming crab juveniles to various levels of cholesterol supplementation on whole body composition (dry matter %) abc ab bc abc c a ab ab ab ab a b (n=3), (P<0.05)

5 ,, Briggs (1988), 0.12%, (Kanazawa, 2001) 3 H,, (D Abramo et al, 1985),, Briggs (1988) Teshima (1994), (Robbins, 1986), 0.6%, 0.6%, 4 0.6%,,, , 29(11): , :, 185,,, , 19(2): Akiyama D M, Dominy W G, Lawrence A L, Penaeid Shrimp Nutrition. In: Fast A W, Lester L J ed. Marine Shrimp Culture: Principles and Practices. Elsevier Science, Amsterdam, Briggs M R P, Jauncey K, Brown J H, The cholesterol and lecithin requirements of juvenile prawn (Macrobrachium rosenbergii) fed semi-purified diets. Aquaculture, 70: Chen H-Y, Requirements of marine shrimp, Penaeus monodon, juveniles for phosphatidylcholine and cholesterol. Aquaculture, 109: Conklin D E, D Abramo L R, Bordner C E et al, A successful purified diet for the culture of juvenile lobsters: The effect of lecithin. Aquaculture, 21: D Abramo L R, Baum N A, Bordner C E et al, Dietdependent cholesterol transport in the American lobster. J Exp Mar Biol Ecol, 87: Gong H, Lawrence A L, Jiang D-H et al, Lipid nutrition of juvenile Litopenaeus vannamei: I. Dietary cholesterol and de-oiled soy lecithin requirements and their interaction. Aquaculture, 190: Harrison K E, The role of nutrition in maturation, reproduction and embryonic development of decapod crustaceans: a review. J Shellfish Res, 9: 1 28 Hernández P V, Olvera-Novoa M A, Rouse D B, Effect of dietary cholesterol on growth and survival of juvenile redclaw crayfish Cherax quadricarinatus under laboratory conditions. Aquaculture, 236: Holme M-H, Zeng C, Southgate P C, The effects of supplemental dietary cholesterol on growth, development and survival of mud crab, Scylla serrata, megalopa fed semipurified diets. Aquaculture, 261: Irvin S J, Williams K C, Barclay M C et al, Do formulated feeds for juvenile Panulirus ornatus lobsters require dietary cholesterol supplementation? Aquaculture, 307: Ishikawa Koshio S, Kanazawa A, Assessment of cholesterol requirements in the prawn, Penaeus japonicus. Aquaculture Nutrition, 3: Kanazawa A, Sterols in marine invertebrates. Fisheries Science, 67: Kean J C, Castell J D, Boghen A G et al, A re-evaluation of the lecitihin and cholesterol requirements of juvenile lobster (Homarus americanus) using crab protein-based diets. Aquaculture, 47: Lovell T, Nutrition and Feeding of Fish. Van Nostrand Reinhold, New York, US: Mercer L, The qualitative nutrient-response relationship. J Nutr, 112: Robbins K R, A method, SAS program, and example for fitting the broken line to growth data. Univ of Tenn Res Rep Univ of Tennessee Agric Exp Sta, Knoxville Sheen S-S, Dietary cholesterol requirement of juvenile mud crab Scylla serrata. Aquaculture, 189: Sheen S-S, Liu P-C, Chen S-N et al, Cholesterol requirement of juvenile tiger shrimp (Penaeus monodon). Aquaculture, 125: Teshima S, Lipid. In: Yone Y ed. Fish Nutrition and Diets. Suisangaku Series, Koseisha-Koseikaku, Tokyo, Japan, 54: Teshima S, Sterols of crustaceans, molluscs, and fishes. In: Patterson G W, Nes W D eds. Physiology and Biochemistry of Sterols. American Oil Chemist s Society, Champaign, II, Teshima S, Phospholipids and sterols. In: D Abramo L, Conklin D E, Akiyama D M ed. Crustacean Nutrition. The

6 6 : (Portunus trituberculatus) 1529 World Aquaculture Society, Baton Rouge, LA, Teshima S, Ishikawa M, Koshio S et al, Necessity of dietary cholesterol for the freshwater prawn, Macrobrachium rosenbergii. Fish Sci. The Abstract of the Autumn Meeting of Japanese Society for Scientific Fisheries, 7 Teshima S, Kanazawa A, Biosynthesis of sterols in the lobster, Palinurus japonica, the prawn, Penaeus japonicus, and crab, Portunus trituberculatus. Comp Biochem Physiol, Thongrod S, Boonyaratpalin M, Cholesterol and lecithin requirement of juvenile banana shrimp, Penaeus merguiensis. Aquaculture, 161: NEEDS OF DIETARY CHOLESTEROL FOR JUVENILE SWIMMING CRAB PORTUNUS TRITUBERCULATUS HAN Tao 1, 2, WANG Ji-Teng 1, HU Shui-Xin 1, LI Xin-Yu 1, LI Rong-Hua 2, MU Chang-Kao 2, WANG Chun-Lin 2 (1. Fisheries College of Zhejiang Ocean University, Zhoushan, ; 2. School of Marine Science of Ningbo University, Ningbo, ) Abstract To investigate cholesterol requirement by juvenile swimming crab Portunus trituberculatus, six diet groups were set up in one-way analysis model for adding 0.003%, %, 0.655%, 0.986%, 1.303%, and 1.628% cholesterol into diet, respectively. Swimming crabs in initial weight about 20g were fed for 8 weeks. Results show that at the end of the experiment, the survival rate was not affected significantly by cholesterol enrichment in all six groups. Weight gain responded linearly to dietary cholesterol supplement up to 0.655% and then went down. Broken-line analysis indicated that dietary cholesterol of 0.6% satisfied the growth performance. At dietary cholesterol supplement 0.655%, crab had the highest weight gain, gland sex index, and carapace width gain. Therefore, we believe that, the optimal dietary cholesterol requirement by juvenile swimming crab was approximately 0.6%. Key words swimming crab Portunus trituberculatus; cholesterol; broken-line; requirement

522 42, (Procambarus clarkii), 7g, (24% 27% 30%), 3 (4% 7% 10%), 9, 4, ,, 2mm, 4 1.3, ( )g 432, 36 ( cm, ), 10 ( : =

522 42, (Procambarus clarkii), 7g, (24% 27% 30%), 3 (4% 7% 10%), 9, 4, ,, 2mm, 4 1.3, ( )g 432, 36 ( cm, ), 10 ( : = 42 4 Vol.42, No.4 2011 7 OCEANOLOGIA ET LIMNOLOGIA SINICA July, 2011 (Procambarus clarkii) * 1 1 2 1 1 1 1 (1. 210095; 2. 210017) 3 3, [ (7.03 0.05)g] 3 (24% 27% 30%) 3 (4% 7% 10%), 9, 4, 10, 8, (P>0.05),

Microsoft Word - 17 韩 涛.doc

Microsoft Word - 17 韩涛.doc