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1 Chinese Journal of Applied Entomology 2017, 54(4): DOI: /j.issn * 补充营养及产卵底物颜色对粘虫生殖的影响 ** 赵玉婉成卫宁 *** 仵均祥摘要目的 Mythimna separatawalker 方法 5%10%10%0.2 moll -1 5%+5%5%+5%5%+5% 结果 5%+5% 0.2 moll % 10% 结论 5%+5% 0.2 moll -1 关键词 Effects of supplementary nutrients and the background color of oviposition sites on the reproduction of Mythimna separata (Walker) ZHAO Yu-Wan ** CHENG Wei-Ning WU Jun-Xiang *** (State Key Laboratory of Crop Stress Biology in Arid Areas (Northwest A&F University), Yangling , China) Abstract [Objectives] To explore the effect of supplementary nutrients, and the background color of oviposition sites, on the reproduction of adult Mythimna separata (Walker) in order to provide a guide for rearing this species in the laboratory. [Methods] Eight kinds of supplementary nutrients were chosen; 5% honey solution, 10% honey solution, 10% glucose solution, 0.2 moll -1 maltose solution, 5% glucose+5% honey mixture, 5% fructose+5% honey mixture, 5% glucose+5% fructose mixture, and a mixture of sugar-acetic acid-ethanol (3 parts white sugar, 1 part white vinegar, 4 parts white wine and 2 parts water). Six colored, plastic ropes; red, yellow, green, blue, purple and white, were selected to test the color preferences of females during oviposition. The study included the pre-oviposition and oviposition periods, and measured adult longevity and fecundity under the different treatments. [Results] Adults fed on the 5% glucose + 5% fructose mixed solution and the 0.2 moll -1 maltose solution, had the highest fecundity, whereas that of those fed on sweet and sour wine was significantly lower than all other treatments. No significant differences was found among the other five supplementary nutrient treatments. Of the six different colors tested, adults preferred blue, and the fecundity of adults was significant higher on blue than on other colors. Fecundity was lowest on red and white and intermediate on yellow, green and purple. [Conclusion] A solution of 5% glucose +5% fructose, or 0.2 moll -1 maltose, and a blue background, are the optimal nutrient and color conditions for the reproduction and growth of M. separata under laboratory conditions. Key words supplementary nutrient, color, selection, Mythimna separata, reproduction * Supported projects ** First author jeannazhao@126.com *** Corresponding author junxw@nwsuaf.edu.cn Received Accepted

2 610 Chinese Journal of Applied Entomology 54 Mythimna separatawalker Prokopy and Owens1983 Phyllotreta striolata F. Myzus persicaesulzer Liriomyza sativae Blanchard 材料与方法 1.1 供试虫源 % 25 70% 14L.. 10D 6 000~8 000 lx 1.2 补充营养 8 5%10%10% 0.2 moll -1 5%+5% 5%+5%5%+5% % 45% 1.3 产卵底物 13~14 cm 4~5 cm C0M89Y95K0 C6M0Y67K0 C57M89Y95K0C63M0Y10K0 C60M70Y5K10C4M3Y2K 试验方法 17.0 cm11.5 cm 7.0 cm ml ±1 70%±10% 14L.. 10D lx

3 4 : 数据处理 SPSS17.0 Tukey 2 结果与分析 2.1 不同补充营养对粘虫蛾生殖与寿命的影响 1 10% 5%10% 0.2 moll -1 5%+5% 5%+5% 10% 5%+5% 5%+5% 0.2 moll -1 10% 0.2 moll -1 10% 0.2 moll -1 10% 2.2 不同补充营养对粘虫蛾产卵趋势的影响 1 10% 5%+5% 3 5 d Treatments 表 1 不同补充营养对粘虫蛾生殖与寿命的影响 Table 1 Effects of different nutrition on adult reproduction of Mythimna separata Longevity (d) Average number of Pre-oviposition peroid (d) Oviposition peroid (d) eggs per female A 4.2±0.37Bb 8.8±0.58Aa 778.3±116.53ABab 16.0±1.26Aab 14.2±2.22Aa B 3.6±0.24Bb 6.2±0.49Aa 896.2± ABab 11.8±0.58Ab 12.0±1.38Aa C 5.0±0.45 ABab 9.0±0.89Aa 728.0±173.55ABab 16.4±0.51Aa 13.2±1.77Aa D 3.8±0.37Bb 11.0±1.58Aa ±159.57Aa 17.2±2.25Aa 17.2±1.93Aa E 6.4±0.24Aa 10.8±0.58Aa 430.0±73.41 Bb 17.4±0.68Aa 15.8±1.56Aa F 4.0±0.32Bb 13.2±1.02Aa 763.4± ABab 14.4±1.21Aab 13.6±1.60Aa G 4.0±0.45Bb 12.9±0.37Aa 926.5±166.36ABab 14.8±1.02Aab 14.4±1.69Aa H 4.6±0.51ABb 15.8±1.84ABab ±153.65Aa 15.2±1.98Aab 16.2±1.83Aa A5%B10%C10%D0.2 moll -1 E F5% +5%G5%+5%H5%+5%± P<0.05Tukey P<0.01Tukey A: 5% honey solution; B: 10% honey solution; C: 10% glucose solution; D: 0.2 moll -1 maltose solution; E: Mixture of sugar-acetic acid-ethanol; F: 5% glucose +5% honey mixture; G: 5% fructose +5% honey mixture; H: 5% glucose +5% fructose mixture. Data in the table are mean ±SE, and followed by the different lowercase letters in the same column indicate significant difference at 0.05 level, while followed by the captical letters indicate extremely significant difference at 0.01 level by Tukey-test. The same below.

4 612 Chinese Journal of Applied Entomology 54 1 Fig. 1 Oviposition of Mythimna separata female adult fed on different supplementary nutrients A5%B10%C10%D0.2 moll -1 E F5%+5%G5%+5%H 5%+5% A: 5% honey solution; B: 10% honey solution; C: 10% glucose solution; D: 0.2 moll -1 maltose solution; E: Mixture of sugar-acetic acid-ethanol; F: 5% glucose + 5% honey mixture; G: 5% fructose + 5% honey mixture; H: 5% glucose + 5% fructose mixture % 80.87% 10% 8 d 5%+5% moll 不同颜色产卵底物对粘虫产卵选择性的影响 2 6 5% 10% 结论与讨论 Treatments Table 2 Red 表 2 不同颜色产卵底物对雌蛾产卵选择性的影响 Influence of colorful substrates on oviposition of Mythimna separata female adult Yellow Green Blue Purple White A 58.6±30.74 ABb 111.4±34.54ABab 168.3±67.49ABab 309.2± Aa 68.1±42.01ABab 60.1±16.08ABb B 18.4±14.81 Bb 160.6±41.98 Bb 161.2±75.38 Bb 409.8±58.09Aa 86.2±18.40Bb 44.3±18.14 Bb C 19.3±12.10 Bb 136.7±29.48ABab 85.2± ABb 257.0±44.96 Aa 138.6±53.17ABab 56.5±38.42Bb D 3.2±2.70Bc 226.3±61.54 Bb 220.2±33.37 Bbc 577.5±91.76 Aa 112.9±46.67 Bbc 80.1±31.30Bbc E 28.5±11.89 Bb 78.5±6.03 Bb 38.7±2.00 Bb 190.2±46.59 Aa 54.0±13.22Bb 23.7±8.93 Bb F 15.8±10.15 Bb 116.7±19.91 Bb 121.1±37.58 Bb 365.8±63.58 Aa 47.5±21.19 Bb 73.6±52.61 Bb G 13.1±12.43 Bb 114.7±43.15 Bb 138.5±40.63Bb 489.9±127.79Aa 99.3±21.16 Bb 67.1±518.97Bb H 15.7±5.04Bb 237.0±66.83 Bb 208.3±63.65Bb 628.3±78.42 Aa 178.2±69.67Bb 39.5±15.09Bb

5 4 : %+5% 0.2 moll % +5% 0.2 moll % 5% 5% Spodoptera exempta (Walker) Hunn and Gate moll -1 5%+5% % 10% 10% Kot1993 Finch and Collier 参考文献 (References) Agricultural Entomology Research Group of Northwestern Agricultural College, Agricultural Entomology (Vol. 2). Beijing: People's Education Press. 69. [, (). :. 69.] Bao XW, Zheng F, Cai MF, Wu JX, Effects of complementary nutrients on adult s reproduction and longevity of oriental fruit moth, Grapholitha molesta Busck. Journal of Northwest A & F University (Nat. Sci. Ed.), 38(8): [,,,, (), 38(8): ] Cao Y, Liu Y, Wang C, Xiong ZL, Li C, The selectivity of Frankliniella occidentalis for the color and volatiles of four host

6 614 Chinese Journal of Applied Entomology 54 plants. Chinese Journal of Applied Entomology, 52(2): [,,,,, , 52(2): ] Dai XF, Ye ZH, Cao YZ, Guo YY, Disaster causing characters and disaster-reducing strategies of crop pests in China. Chinese Journal of Applied Entomology, 10(1): [,,,, , 10(1): ] Fu JW, Xu DM, Wu W, You MS, Preference of different vegetable insect pests to color. Chinese Bulletin of Entomology, 42(5): [,,,, , 42(5): ] Finch S, Collier RH, Host-plant selection by insects-a theory based on appropriate/inappropriate landings by pest insects of cruciferous plants. Ent. Exp. & Appl., 96(2): Guo F, Liu JL, Study on reproduction of armyworm, Mythimna separata (Walker) Ⅱ. Effect of supplementary nutrition on reproduction. Acta Entomologica Sinica, 13(6): [,, Ⅱ.., 13(6): ] Gunn A, Gatehouse AG, The influence of larval phrase on metabolic reserves, fecundity and lifespan of the African armyworm moth, Spodoptera exempta (Walker) (Lipidoptera: Noctuidae). Bull. Ent. Res., 77(4): Jiang XF, Yao R, Zhang L, Cheng YX, Liu YQ, Luo LZ, Effects of supplementary nutrition on adult reproduction and longevity of Athetis lepigone (Möschler). Journal of Plant Protection, 42(6): [,,,,,, , 42(6): ] Jiang XF, Zhang L, Cheng YX, Luo LZ, Current status and trends in research on the oriental armyworm, Mythimna separata (Walker) in China. Chinese Journal of Applied Entomology, 51(4): [,,,, , 51(4): ] Kot Ⅳ, Physical and chemical factors influencing landing and oviposition by the cabbage root fly on the host-plants models. Ent. Exp. & Appl., 66: Lin CC, Lai MH, Lu G, Cai DY, Zhou CM, Zhao JN, Effect of supplementary nutrition materials on Monochamus alternatus (Hope) female adult fecundity. Forest Research, 16(4): [,,,,,, , 16(4): ] Luo C, Cao YZ, Li KB, Effect of supplementary nutrition on Mythimna separata (Walker) moth energy substance content. Acta Entomologica Sinia, S(1): [,,, , S(1): ] Prokopy RJ, Owens ED, Visual detection of plants by hebivious insects. Annu. Rev. Entomol., 28: Qin DR, Guo TB, Jiang FR, Zhou Y, Zhao HZ, Wang KF, Studies on the relationship between Apriona germari (Hope) ovary development and nutritional supplement. Journal of Nanjing Forestry University, 18(3): [,,,,,, , 18(3): ] Qin JD, Wei DY, Wang ZS, Study on the nutrition of Mythimna separata (Walker)-feeding and utilization of carbohydrate by adults. Acta Entomologica Sinia, 13(6): [,,, , 13(6): ] Wu JX, Agricultural Entomology (northern book plant protection professional2 nd edition). Beijing: China Agricultural Science and Technology Publishing House Press [, (). : ] Wu QY, Guo F, Effect of lateral pharyngeal on armyworm ovarian development and maturation. Acta Entomologica Sinia, 12(4): [,, , 12(4): ] Yin J, Xue YG, Qiao HB, Li KB, Hu Y, Cao YZ, The significance of oviposition site selection and effect of color in orientation by oriental armyworm Mythimna separata Walker. Acta Ecologica Sinica, 27(6): [,,,,,, (Mythimna separata Walker)., 27(6): ] Yang XF, Ma CS, Fan F, Liu YF, Feng N, Li Q, Wei GS, Effect of colours on oviposition preference of the oriental fruit moth, Grapholita molesta Busck. Acta Ecologica Sinica, 34(11): [,,,,,,, , 34(11): ]

168 健等木醋对几种小浆果扦插繁殖的影响第1期 the view of the comprehensive rooting quality, spraying wood vinegar can change rooting situation, and the optimal concent

168 健等木醋对几种小浆果扦插繁殖的影响第1期 the view of the comprehensive rooting quality, spraying wood vinegar can change rooting situation, and the optimal concent 第 31 卷第 1 期 2013 年 3 月经济林研究 Nonwood Forest Research Vol. 31 No.1 Mar. 2013 木醋对几种小浆果扦插繁殖的影响健 1,2 杨国亭 1 刘德江 2 (1. 东北林业大学生态研究中心黑龙江哈尔滨 150040 2. 佳木斯大学生命科学学院黑龙江佳木斯 154007) 摘要为了解决小浆果扦插繁殖中生根率及成活率低等问题