4 : cm 5% 1.. 粘虫表型的观察遗传模式的确定正常型的雌雄和突变体的雌雄分别配对杂交的 F1 代观察材料与方法虫源

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1 Chinese Journal of Applied Entomology 014, 51(4): DOI: /j.issn 一种深色型粘虫成虫体色突变体的遗传分析 * 李栋张云慧李祥瑞程登发 ** 摘要目的 Mythimna separate Walker 方法结果 F 1 F F 结论关键词 Genetic analysis of a Mythimna separate (Walker) body color mutant LI Dong ZHANG Yun-Hui LI Xiang-Rui CHENG Deng-Fa ** (Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing , China) Abstract [Objectives] Body color mutants of Mythimna separate (Walker) were found in a laboratory colony and in the wild and their phenotypes observed. [Methods] A hybridization experiment was used to analyze the genetic basis of this mutation. [Results] Compared with normal moths, the adult body of mutants, especially the abdomen and wings, were darker in color. There were no obvious differences in the appearance of eggs, larvae and early pupae, but immediately before eclosion, mutant pupae were darker in color than normal pupae. The results of the hybridization experiment show that the proportion of the normal type in F1 progeny of reciprocal crosses between normal and mutant parents was 100%, whereas the ratio of F segregation between normal and mutant types was The ratio in backcrosses was In addition, progenies of self-crossed F mutants were exclusively of mutant type. [Conclusion] The results of the hybridization experiment are consistent with Mendelian, monogenic autosomal recessive inheritance. These results shed light on the molecular mechanism determining moth body color differentiation at both the genetic and environmental levels and contribute to the theoretical basis for the prediction and integrated management of M. separata. Key words Mythimna separata, body color mutant, genetic analysis Mythimna separata Walker * CARS-3 ** dfcheng@ippcaas.cn

2 4 : cm 5% 1.. 粘虫表型的观察遗传模式的确定正常型的雌雄和突变体的雌雄分别配对杂交的 F1 代观察材料与方法虫源两种型的划分 1. 试验条件和方法 1..1 试验条件 3±1 L1.. D1.5 cm 10 cm 10 cm F1 代自交后的 F 代观察 F1 F F1 代与突变体的回交得到 RF1 代观察 F 1 RF1

94 Chinese Journal of Applied Entomology 51 1.3.4 F 代的突变体自交后观察 F F in pupae 结果与分析.1 粘虫表型观察 1 图正常型和突变体粘虫成虫体色比较 Fig.

1 Comparison of typical form and mutant type F1 F1 F 1 Groups 表 1 4 组杂交试验及 F 1 代自交后代的成虫体色 Table 1 Body color in four hybrid progenies and

3 94 Chinese Journal of Applied Entomology F 代的突变体自交后观察 F F in pupae 结果与分析.1 粘虫表型观察 1 图正常型和突变体粘虫成虫体色比较 Fig. Comparison of phenotype of typical form and mutant in Mythimna separata. 正常型和突变体成虫系列杂交试验结果图 1 突变体和正常型羽化前蛹体色比较 Fig. 1 Comparison of typical form and mutant type F1 F1 F 1 Groups 表 1 4 组杂交试验及 F 1 代自交后代的成虫体色 Table 1 Body color in four hybrid progenies and self-crossed progenies of F 1 Typical form Mutant Real ratio Theoretical ratio Yn=1Y F 1 F =3.84

4 4 : =3.84. Both the actual ratio and theoretical ratio are the number of mutant to typical form. The same below. \.3 F 代突变体自交后代及 F1 代与突变体回交的 BF1 代体色结果 F1 F F 3 讨论 Kettlewell 1961 Majerus 1998 Helicoverpa armigera 005 Drosophila melanogaster Table 表 F 代突变体自交后代及 F 1 代与突变体回交的 RF 1 代体色 Body color in self-crossed progenies of F mutant and the RF1, hybrid F 1with the mutant Group Typical form Mutant Real ratio Theoretical ratio Yn=1Y F 1 RF

5 96 Chinese Journal of Applied Entomology 51 F 参考文献 (References) Kettlewell HBD, The phenomenon of industrial melanism. Annu. Rev. Entomol., 6: Majerus MEN, Melanism: Evolution in Action. Oxford: Oxford University Press. 1 1.,, : [Dai YJ, Wang LS, The analysis method of genetic. Changsha: Hunan Technology Press. 7-44],,,,, 007.., 44(3): [Du GL, Li KB, Yin J, Liu H, Cao Y, 007. The dominant ecological factors in color change of Macrosiphum avenae. Chinese Bulletin of Entomology, 44(3): ],,,,,,, 010.., 4(1): [He LH, Niu BL, Liu Y, Shen WF, Weng HB, Chen JE, Meng ZQ, 010. Genetic analysis of a moth body colour mutant in helicovrpa armigera. Acta Agriculturae Nucleatae Sinica, 4(1): ],, 007.., 50(11): [Jiang XF, Luo LZ, 007. Melanism in insects: a review. Acta Entomologica Sinica, 50(11): ],, : [Kong FL, Han LX, et al, The introduction of population genetics, Beijing: Beijing Agricultural University Press], , 19(4): 4. [Li GB, The research condition and main progress of armyworm in China. Plant Protection, 19(4): -4], : [Lin SC, Physiological ecology of armyworm. Beijing; Beijing University Press]., :. [Liu HB, 00. Strategy and adaptive capacity in the melanic form of oriental armyworm, mythimna separata (Walker) (Lepidoptera: Noctuidae). Ph. D. Dissertation. Beijing : Chinese Academy of Agricultural Sciences.], 004.., 47(3): [Liu HB, Luo LZ, 004. Morphological characteristics and inheritance of the melanic for m of the oriental armyworm, mythimna separata (Walker) (Lepid optera: Noctuidae). Acta Entomologica Sinica, 47(3): 87-9.],,,, , 38(1): [Luo LZ, Li GB, Cao YZ, Hu Y, The influence of larval rearing density on flight capacity and fecundity of adult oriental armyworm. Acta Entomologica Sinica, 38(1): 38-45], 005. Helicoverpa armigera (Hbner). :. [Ma WH, 005. Study on genetic regulation and correlation enzyme of the black phenotype mutants of moth and pupa of helicoverpa armigera (Hübner). Master Degree Thesis. Wuhan: Huazhong Agricultural University.],, , 9(11): [Pan L, Zhai BP, 009. Analysis of source population and immigration process of mythimna separata (Walker) caused an outbreak in north China in 00. Acta Ecologica Sinica, 9(11): ],,,, 004. (Drosophila melanogaster). ( ), 16(3): [Qian YH, Zhang P, Xue XQ, Xie AH, 004. Preminary study and discovery of black streak body mutant of Drosophila melanogaster. Hubei University Press (Natural Science Edition), 16(3): ],,, ( ), 0(1): [Qian YH, Zhang P, Zeng QT, Discovery, Selectivity Breeding and Use of New-Strains in Local Drosophila melanogaster. Hubei University Press (Natural Science Edition), 0(1): ],,, 000.., 3(4): 9 1. [Yang MG, Chen YY, Jiang LJ, 000. Discovery of melanism mutation in silkworm

6 4 : 97 bombyx mori and the possibility of its practical utilization. Bulletin of Sericulture, 3(4): 9-1.],,,,,, , 38(5): 1 8. [Zhang YH, Zhang Z, Jiang YY, Zeng J, Gao YB, Cheng DF, 01. Preliminary analysis of the outbreak of the third-generation army worm mythimna separata in China in 01. Plant Protection, 38(5): 1-8.],,,,, , 36(3): [Zhao HY, Zhang GS, Wang SZ, Wang YM, Basang PC, An eco-genetical study of body colour in cotton aphids. Acta Entomologica Sinica, 36(3): 8-89].,, 1964.,,, ( ) [Zhu HF, Chen YX, The economic important insect of China, Volume 3, Lepidoptera: Noctuidae (1). Beijing: Science Press ]

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4 : cm 5% 1.. 粘虫表型的观察 遗传模式的确定 正常型的雌雄和突变体的雌雄分别配对杂 交的 F1 代观察 材料与方法 虫源

4 : cm 5% 1.. 粘虫表型的观察遗传模式的确定正常型的雌雄和突变体的雌雄分别配对杂交的 F1 代观察材料与方法虫源