Microsoft Word 盖圣美-二校

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1 7 6 Vol. 7 No Journal of Food Safety and Quality Jun., 2016 盖圣美 1, 魏法山 2, 刘登勇 1*, 巩阿娜 (1.,, ; 2., ) 1 摘要 :,, 3,,, 关键词 : ; ; ; Research progress of clenbuterol residues detection methods GAI Sheng-Mei 1, WEI Fa-Shan 2, LIU Deng-Yong 1*, Gong A-Na 1 (1. College of Food Science and Technology, Bohai University; Food Safety Key Lab of Liaoning Province; National & Local Joint Engineering Research Centre of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou , China; 2. Henan Province Product Quality Supervision and Inspection Center, Zhengzhou , China) ABSTRACT: Clenbuterol, a kind of β-adrenergic agonist, is not only widely used as a bronchodilator in clinic, but also illegally used as growth promoters in animal production. It was forbidden due to the poison of long-term consumption of clenbuterol. The present paper mainly introduced the domestic and foreign current detecting standards and methods of clenbuterol, ractopamine and salbutamol. The advantages and disadvantages of each kind of drugs detection method were analyzed and evaluated. It aimed at providing references for food safety workers and researchers, and laying a good foundation to the rapid development of meat industry and the safeguard of people s safety. KEY WORDS: clenbuterol; ractopamine; salbutamol; foodstuffs of animal origin 1 引言, [1,2],,,,, [3], 9% 16%, 10% 15%, [4], [5],, * 通讯作者 :,,, jz_dyliu@126.com *Corresponding author: LIU Deng-Yong, Ph.D., Associate Professor, Bohai University, No.19, Keji Road, New Songshan District, Jinzhou , China. jz_dyliu@126.com

2 6, : 2297, 160 [6] ,,,,, [7] - [7] - [8] - [9], [10] [11],,,, 2 我国现行有效的瘦肉精类药物标准检测方法 , 1997,,,,,,,, - ( : 0.5 µg/kg [7] ; 0.10 µg/kg [12] ; 0.05 µg/kg [13] ; 0.25 µg/kg [14] ) - ( : 0.5 µg/kg [7] ; 1.0 µg/kg [9] ; 2.0 µg/kg [15, 16] ) ( : 0.5 µg/kg [7] ) ( : 0.5 µg/kg [7] ) 4 - -,,, / ( ) ( ) 4, - ( : 0.5 µg/kg [8] ; 0.10 µg/kg [12] ; 0.5 µg/kg [17] ; 0.25 µg/kg [18] ) - ( : : mg/kg : mg/kg [19] ; 2.0 µg/kg [8] ) ( : mg/kg [17] ) ( : : 1.5 µg/kg : 1.4 µg/kg : 1.4 µg/kg [20] ),, - -,, β-, - ( : 0.5 µg/kg [8] ; 0.10 µg/kg [12] ; 0.05 µg/kg [13] ; 0.25 µg/kg [18] ) - ( : 1.0 µg/kg [8] ), - -,,,,,,,,,,,,, 3 瘦肉精类药物新型检测方法研究现状 3.1 克仑特罗, [21], ; ;,, [22-24],, [25] ( : 1 µg/kg), [26], Wang [27] (UCP) (ICA) (CL), 10 min, 0.1 ng/ml,, [28] T/C, ELISA,

3 (R 2 =0.9136), Tang [29],,, [30,31], / [32,33], Xu [34] - (LC-MS/MS), 0.32 ng/g 0.36 ng/g [35,36] 3.2 莱克多巴胺 β-, 5~10, [37],,, [38],, 160, 133, ; 27 ( ) [33] Nino [39],,, - -,,, Xiao [40] ( ), 3.1~4.3 μg/l, 72.4%~79.7%,, β- Chen [41] QCM, 0.01 ng/ml,, 92.3%~110%,,, [42] [43] [44-47] 3.3 沙丁胺醇,, [48] - -, [49] ( 2 ng/ml), 2.67%, 3 min,,,,, Lin [50], 0.05 µmol/l 0.1 µmol/l, 98.7% 97.1% Rajkumar [51],, 4 存在问题 - - -,,,,,,, : (1),, ; (2), ; (3),,,,,,,,,,,,,,,,,,,,

4 6, : 结论,,,,,,, ;,, ( ) [39],,,, β- 参考文献 [1] Blanca J, Muñoz P, Morgado M, et al. Determination of clenbuterol, ractopamine and zilpaterol in liver and urine by liquid chromatography tandem mass spectrometry [J]. Anal Chim Acta, 2005, 529(1 2): [2] Bi S, Bo P, Wang T, et al. Investigation on the interactions of clenbuterol to bovine serum albumin and lysozyme by molecular fluorescence technique [J]. Spectrochim Acta Part A Mol Biomol Spectrosc, 2014, 120(4): [3], 晞,,. [J]., 2013, (7): Xin CX, Shao X, Meng XS, et al. Contrast analyses of common clenbuterol detection method [J]. Chin Anim Husb Vet, 2013, (7): [4],,,. [J]., 2011, 28(4): 4 6. Lu P, Xiao X, ZhangYH, et al. China s clenbuterol supervision situation analysis and countermeasures suggestions [J]. China Anim Health Inspect, 2001, 28(4): 4 6. [5],,,. [J]., 2014, 39(1): 23, 27. Fan RH, Luo YJ, Zhou SS, et al. Discussion on how to clenbuterol residues detection in pig [J]. Guangdong J Anim Vet Sci, 2014, 39(1): 23, 27. [6]. GC/MS [D]. :, Zhang R. Research of detection technology of three kinds of clenbuterol by GC/MS and application [D]. Nanning: Guangxi University, [7] GB/T [S]. GB/T Determination of clenbuterol residues in animal food [S]. [8] GB/T β-. [S]. GB/T Determination of residual amounts of β-agonists in animal derived foods by liquid chromatography mass [9] β-. - [S]. department announcement No Pig liver and urine β-agonist residues detection by gas chromatography mass [10],,,. [J]., 2013, 39(4): Li CH, Chen XL, Guo L, et al. Development of a colloidal gold immunochromatographic assay for rapid and quantitative detection of clenbuterol residues in pork [J]. Food Ferment Ind, 2013, 39(4): [11] Huy BT, Seo MH, Zhang X, et al. Selective optosensing of clenbuterol and melamine using molecularly imprinted polymer-capped CdTe quantum dots [J]. Biosens Bioelectron, 2014, 57C(10): [12] GB/T β-. - [S]. GB/T Detection method for beta receptor agonist residues in animal derived foods by liquid chromatography mass [13] SNT [S]. SNT The food of animal origin for import and export of clenbuterol, ractopamine, salbutamol and terbutaline residues determination by liquid chromatography mass [14] β-. [S]. department announcement No Detection of β-agonists residues in animal derived foods by liquid chromatography mass [15] NYT [S]. NYT Determination of clenbuterol hydrochloride in animal tissues by gas chromatography mass [16] [S]. department announcement No Detection methods of clenbuterol residue in cattle edible tissues by gas chromatography mass [17] [S].. department announcement No Ractopamine in animal derived food by liquid chromatography mass [18] β-. - [S]. department announcement No Determination of residual β-agonists in animal derived foods by liquid chromatography mass [19] [S]. department announcement No Detection of ractopamine

5 residues in animal tissues and animal urine by gas chromatography mass [20] [S]. department announcement No Detection of ractopamine residues in animal food by ELISA [S]. [21],,,. [J]., 2014, (5): Sun LY, Yin L, Yao T, et al. Research on immunochip techniques for residue detection of clenbuterol [J]. Chin J Pharm Anal, 2014, (5): [22] Pfaffl MW, Ginkel LAV, Mcevoy JD, et al. Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 2: Certification [J]. Fresenius J Anal Chem, 2001, 371(8): [23] Zhang Y, Chen YN, Zheng XZ, et al. Tissue distribution of clenbuterol at toxic and therapeutic dosage in rabbits [J]. Chin J Hospital Pharm, 2007, 27(5): [24] Yan K, Fu LL, Hong XF, et al. Acute clenbuterol induces hypotension, atrioventricular block and cardiac asystole in the rabbit [J]. Cardiov Toxicol, 2013, 13(1): [25] Ming-Zhou Z, Min-Zi W, Zong-Lun C, et al. 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Food Control, 2015,22(6): [40] Xiao X, Yan K, Xu X, et al. Rapid analysis of ractopamine in pig tissues by dummy-template imprinted solid-phase extraction coupling with surface-enhanced Raman spectroscopy [J]. Talanta, 2015, 138: [41] Chen S, Pan D, Gan N, et al. A QCM immunosensor to rapidly detect ractopamine using bio-polymer conjugate and magnetic β-cyclodextrins [J]. Sensors Actuat B Chem, 2015, 211: [42] Baytak AK, Teker T, Duzmen S, et al. A novel voltammetric sensor based on carbon nanotubes and nanoparticles of antimony tin oxide for the determination of ractopamine [J]. Mater Sci Eng C, 2016, 59: [43] Liu Z, Zhou Y, Wang Y, et al. Enhanced oxidation and detection of toxic ractopamine using carbon nanotube film-modified electrode [J]. Electrochim Acta, 2012, 74(4): [44] Suo D, Zhao G, Wang R, et al. Determination of ractopamine in animal hair: application to residue depletion in sheep and residue monitoring [J]. 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6, : 2301, 2012, 28(11): 1595 1599. Wu QL, Ye CS. Colloidal gold immunochromatographic assay for residual sabutamol [J]. Mod Food Sci Technol, 2012, 28(11): 1595 1599.

Sensors Actuat B Chem, 2013, 177(2): 428 436. [51] Rajkumar M, Li YS, Chen SM.

6 6, : 2301, 2012, 28(11): Wu QL, Ye CS. Colloidal gold immunochromatographic assay for residual sabutamol [J]. Mod Food Sci Technol, 2012, 28(11): [50] Lin KC, Hong CP, Chen SM. Simultaneous determination for toxic ractopamine and salbutamol in pork sample using hybrid carbon nanotubes [J]. Sensors Actuat B Chem, 2013, 177(2): [51] Rajkumar M, Li YS, Chen SM. Electrochemical detection of toxic ractopamine and salbutamol in pig meat and human urine samples by using poly taurine/zirconia nanoparticles modified electrodes [J]. Colloids Surfaces B Biointerfaces, 2013, 110(10): ( 责任编辑 : 李振飞 ) 作者简介盖圣美, 讲师, 主要研究方向为食品安全与分析 gaishengmei@126.com 刘登勇, 博士, 副教授, 主要研究方向为肉品质量安全控制 jz_dyliu@126.com

Microsoft Word 张璐琪-二校

Microsoft Word 张璐琪-二校 7 5 Vol. 7 No. 5 2016 5 Journal of Food Safety and Quality May, 2016 3 β- 张璐琪 1*, 王凤美 2, 张鸿伟 2, 徐彪 2, 梁成珠 (1., 266003; 2., 266002) 2 摘要 : 目的 3 - 方法 (CLB) (RAC) (SAL)3 β-, - (LC-MS/MS) 结果 - - LC-MS/MS 83.3