《中国科学》A、E、G与F小开本版式设计

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1 222 E 2006, 36(2): 222~234 * 1** 1,2 1,2 1,2 1, ,2 (1., ; 2., ; 3., ),. ;,, (human genome project, HGP), ; HGP 2003, 21. (proteomics) ; (computational proteomics),,, [1].,,,. : ; : * 973 (2002CB713807) (2004BA711A21) ** [email protected]

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12 2 : 233,. 5,,,,,,, ;,. KSDP pfind,,.,,,,.,,.,,,,,,,,.,,,,,..,,., pfind. 1 Patterson S D, Aebersold R H. Proteomics: the first decade and beyond. Nature Genetics Supplement, 2003, 33:311~323[DOI]

13 234 E 36 2 Johnson R S, Davis M T, Taylor J A, et al. Informatics for protein identification by mass spectrometry. Methods, 2005, 35: 223 ~236[DOI] 3 Aebersold R H, Mann M. Mass spectrometry-based proteomics. Nature, 2003, 422:198~207[DOI] 4 Steen H, Mann M. The ABC's(and XYZ's) of peptide sequencing. Nature Reviews Molecular Cell Biology, 2004, 5:699~711[DOI] 5 Russell S A, Old W, Resing K A, et al. Proteomic informatics. International Review of Neurobiology, 2004, 61:129~157 6 Fu Y, Yang Q, Sun R, et al. Exploiting the kernel trick to correlate fragment ions for peptide identification via tandem mass spectrometry. Bioinformatics, 2004, 20:1948~1954[DOI] 7 Eng J K, McCormack A L, Yates J R. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. Journal of American Society of Mass Spectrometry, 1994, 5: 976~989[DOI] 8 Perkins D N, Pappin D J, Creasy D M, et al. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis, 1999, 20: 3551~3567[DOI] 9 Fenyö D, Beavis R C. A method for assessing the statistical significance of mass spectrometry-based protein identifications using general scoring schemes. Analytical Chemisty, 2003, 75:768~774[DOI] 10 Li D, Fu Y, Sun R, et al. pfind: a novel database-searching software system for automated peptide and protein identification via tandem mass spectrometry. Bioinformatics, 2005, 21(13): 3049~3050[DOI] 11 Zhang J, Gao W, Cai J, et al. Predicting molecular formulas of fragment ions with isotope patterns in tandem mass spectra. IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2005, 2(3):217~230[DOI] 12 Gygi S P, Rist B, Gerber S A, et al. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nature Biotechnololy, 1999, 17: 994~999[DOI] 13,. SELDI., 2002, 1:1~4 14 Liotta L A, Ferrari M, Petricoin E F. Clinical proteomics: written in blood. Nature, 2003, 425:905[DOI] 15 Petricoin E F, Ardekani A M, Hitt B A, et al. Use of proteomic patterns in serum to identify ovarian cancer. The Lancet, 2002, 359: 572~577[DOI] 16 Wulfkuhle J D, Liotta L A, Petricoin E F. Proteomic applications for the early detection of cancer. Nature Reviews Cancer, 2003, 3: [DOI]

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untitled 19 3 2007 6 Chinese Bulletin of Life Sciences Vol. 19, No. 3 Jun., 2007 1004-0374(2007)03-0281-08 ( 116023) / 2D PAGE - ph Q503; Q816 A New technology and method for proteomics research ZHANG Weibing,