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1 海带粗多糖对斜带石斑鱼血清指标的影响李文武 1, 殷光文 1, 林希 1, 黄志坚 1, 林建斌 2, 江和基 1 2, 朱庆国 (1., ; 2., ) 摘要 : 为了探讨日粮中添加不同剂量的海带粗多糖对斜带石斑鱼 (Epinephelus coioides) 血清指标的影响, 作者选取规格一致的健康斜带石斑鱼 120 尾 ( 平均体质量为 90 g± 2.6 g, 平均体长为 15 cm± 2.25 cm), 均匀分为 4 组, 每组设两个重复, 每个重复 15 尾对照组日粮中不添加海带粗多糖, 试验 0.5% 组 1.0% 组 1.5% 组日粮中分别添加 0.5% 1.0 % 1.5 % 的海带粗多糖经过 48 d 的喂养后, 测定各组斜带石斑鱼血清中白介素 2(Interleukin-2, IL-2) 白介素 6(Interleukin-6, IL-6) 干扰素 α(interferon-α, IFN-α) 总蛋白 (Total Protein, TP) 尿素(UREA) 肌酐(CREA) 碱性磷酸酶(Alkaline phosphatase, ALP) 谷草转氨酶 (Aspartate aminotransferase, AST) 谷丙转氨酶(Alanine aminotransferase, ALT) 溶菌酶(Lysozyme, LZM) 过氧化物酶(Catalase, CAT) 超氧化物歧化酶(Superoxide dismutase, SOD) 补体 3(Complement 3, C3) 补体 4(Complement 4, C4) 等水平结果表明, 海带粗多糖能够不同程度地提高血清中 IL-2 IL-6 IFN-α TP 水平, 及 LZM CAT 和 SOD 酶活性 ; 降低 C3 C4 含量, 及 UREA CREA 水平和 ALP 酶活性 ; 对 AST 和 ALT 酶活性影响不大在 3 种添加比中, 以 0.5% 的海带粗多糖添加剂量效果最明显关键词 : 海带粗多糖 ; 斜带石斑鱼 (Epinephelus coioides); 细胞因子 ; 血清生化 ; 非特异性免疫中图分类号 : S 文献标识码 : A 文章编号 : (2015) doi: //hykx , [1],,, [2], [3], [4-5],, [6], [7-11],, [12],,,, [13] 1 材料与方法 1.1 试验鱼饲养管理, 90 g±2.6 g, 15 cm±2.25 cm, 10 mg/l,, L, 2, 15,, 8: 00~8: 30 17: 00~17: 30, : ; : : ( [2012] 28 ) : (1989-),,,,, : , xiaowen-521@qq. com;,, : , huangzj1999@ sina.com Marine Sciences / Vol. 39, No. 6 /

2 ( 3%),,,,,, 1 h, 1/3~1/2,, 24~30, ph 8.0±0.3, >7.0 mg/l, <0.2 mg/l 48 d 1.2 试验期日粮的配制 ( ),, 38.9% ( ) 0% 0.5% 1.0% 1.5% ( 1), 0.5% 1.0% 1.5% 4 mm,, 4, 3 d 1, 3 d, 表 1 基础日粮营养成分表 Tab. 1 Nutrition of the basal diet (%) 0.5% 1.0% 1.5% : 1.3 样品采集, 24 h,, 10,, 2.5 ml, 4,, 测定指标和方法 IL-2 IL-6 IFN-α ELISA, IDEXX VetTest ( ),,, SOD ; C3 C4 LZM ;AKLP, EXCEL SPSS 19.0 (ANOVE), LSD, ± 2 结果 2.1 海带粗多糖对斜带石斑鱼血清细胞因子水平的影响 2,, IL-2 IL-6 IFN-α 0.5% (P<0.01); 1.0% IL-2 IL-6 (P<0.01), IFN-α (P<0.05); 1.5% IL-2 IL-6 IFN-α, (P>0.05) 2.2 海带粗多糖对斜带石斑鱼血清非特异性免疫的影响 3,, LZM CAT SOD, C3 C4,, CAT SOD, LZM, C3 C4, ; 0.5% LZM (P<0.05), CAT SOD (P<0.01), C3 (P<0.05), C4 (P<0.01); 1.0% CAT (P<0.05), C4 (P<0.05) 2.3 海带粗多糖对斜带石斑鱼血清生化指标的影响 4, 48 d,, TP, 0.5% 1.0% (P<0.01), 1.5% (P<0.05); UREA, 0.5% (P<0.05), 1.0% 60 / 2015 / 39 / 6

3 表 2 海带粗多糖对斜带石斑鱼血清中细胞因子水平的影响 Tab. 2 Effect of laminarin on serum cytokines in Epinephelus coioides 0.5% 1.0% 1.5% IL-2(ng/mL) ± ± A ± A ± IL-6(ng/mL) ± ± A ± A ± IFN-α(ng/mL) ± ± A ± a ± : (P>0.05), (P<0.05), (P<0.01), 3 表 3 海带粗多糖对斜带石斑鱼血清非特异性免疫指标的影响 Tab. 3 Effect of laminarin on non-specific immune response in Epinephelus coioides 0.5% 1.0% 1.5% LZM(U/mL) 46.32± ±19.28a 51.85± ±13.35 CAT(U/mL) 1.12± ±1.27A 1.57±1.40a 1.25±0.62 SOD(mg/mL) 45.68± ±19.49A 53.25± ±14.66 C3(g/L) 0.162± ±0.026a 0.157± ±0.031 C4(mg/L) 9.376± ±0.992A 6.454±0.810a 8.349±1.451 表 4 海带粗多糖对斜带石斑鱼血清生化指标的影响 Tab. 4 Effect of laminarin on plasma biochemical parameters in Epinephelus coioides 0.5% 1.0% 1.5% TP(g/L) 41.13±6.07 Aa 44.38±8.1 Aa 46.37±11.72 Aa 42.75±9.36 Aa UREA(mmol/L) 2.95±0.99 Aa 2.39±0.07 Ab 2.51±0.30 Aa 2.56±0.35 Aa CREA(mmol/L) ± Aa ± Aa ± Aa ± Ab ALP(U/L) ±74.58 Aa ±39.20 Aa ± Aa ±44.83 Aa AST(U/L) ±79.71 Aa ±65.78 Aa ±86.01 Aa ±84.10 Aa ALT(U/L) ±8097 Aa ± Aa ± Aa ± Aa : (P<0.01), (P<0.05), (P>0.05) 1.5% (P>0.05); CREA,, CREA, 1.5% (P<0.05), 0.5% 1.0% (P>0.05); 0.5% 1.5% ALP, 1.0%, (P>0.05); 0.5% AST (P<0.05), 1.0% 1.5% AST, (P>0.05); ALT,, ALT, (P>0.05) 3 讨论 3.1 海带粗多糖对斜带石斑鱼血清细胞因子水平的影响, IL-2 IL-6 IFN-α,,, Ryu [14], T (CTLs) (LAKs), IL-2 Smith [15] 300 mg/l LJPS LPS IL-6(P=0.0289) IL-8(P=0.0245),, IL-2 IL-6 IFN-α, 3,,, IL-2 IL-6 IFN-α, [16-17], Marine Sciences / Vol. 39, No. 6 /

4 3.2 海带粗多糖对斜带石斑鱼血清免疫指标的影响 LZM,,,,,, CAT,,, H 2 O 2 O 2 H 2 O, H 2 O 2, H 2 O 2, SOD,,, SOD Kang [18], SOD CAT Moroney [19],, C3 C4,,,,,, LZM CAT SOD ; C3 C4,,,,, [20] [21], 100 mg/kg, [22], SOD CAT,, ; Zhu [23], Olafsdottir [24] β-, 3.3 海带粗多糖对斜带石斑鱼血清生化水平的影响, UREA CREA, ; ALT AST ALT AST, ALP,, ALP, AST ALT, [12], ALT AST (4<ALT<22, 13<AST<31), 1, AST U/L,, ALT ; 20 ALT, ALT, AST [12] (51~524U/L),,, 1, 4 结论,,,,,,,, 0.5% : [1],,,. [J]., 2001, 20(2): 7-9. [2],,,. [J]., 2001, 8(2): [3],,,. [J]., 2006, 41(1): [4],. [J]., 2003, 22(1): [5],. [J]., 2004, 14(5): [6] Shirosaki M, Koyama T. Laminaria japonica as a food for the prevention of obesity and diabetes[j]. Advances 62 / 2015 / 39 / 6

5 in Food and Nutrition Research, 2011, 64: [7],,,. [J]., 2012, 39(22): [8],,,. [J]., 2011, 26(3): [9],,,. [J]., 2011, 23(9): [10] Alban S, Kraus J, Franz G. Synthesis of laminarin sulfates with anticoagulant activity[j]. Arzneimittel- Forschung, 1992, 42(8): [11]. [J]., 2010, 32(3): [12],,,. [J]. ( ), 2004, 1: [13],,,. [J]., 2011, 30(1): 1-5. [14] Ryu D S, Oh S M, Kim K H, et al. Immunomodulating activity of Laminaria japonica polysaccharides[j]. Korean Journal of Food Science and Technology, 2010, 42(3) : [15] Smith A G, O'Doherty J V, Reilly P, et al. The effects of laminarin derived from Laminaria digitata on measurements of gut health: selected bacterial populations, intestinal fermentation, mucin gene expression and cytokine gene expression in the pig[j]. British Journal of Nutrition, 2011, 105(5): [16],,. LJPS [J]., 2000, 14(2): 75. [17],. LJPS [J]., 2001, 22(3): [18] Kang Y M, Lee B J, Kim J I, et al. Antioxidant effects of fermented sea tangle (Laminaria japonica) by Latobacillus brevis BJ20 in individuals with high level of γ-gt: A randomized, double-blind, and placebocontrolled clinical study [J]. Food and Chemical Toxicology, 2012, 50(3): [19] Moroney N C, O'Grady M N, O'Doherty J V, et al. Addition of seaweed (Laminaria digitata) extracts containing laminarin and fucoidan to porcine diets: Influence on the quality and shelf-life of fresh pork[j]. Meat Science, 2012, 92(4): [20],,. [J]., 2011, 1: [21]. LJPS [J]., 2013, 1: [22],,,.,, [J]., 2011, 30(9): [23] Zhu Y, Pettolino F, Mau S, et al. Immunoactive polysaccharide-rich fractions from Panax notoginseng[j]. Planta Medica, 2006, 72(13): [24] Olafsdottir E S, Omarsdottir S, Paulsen B S, et al. Immunologically active O6-branched (1-3)-β-glucan from the lichen Thamnolia vermicularis var. subuliformis[j]. Phytomedicine, 2003, 10(4): Marine Sciences / Vol. 39, No. 6 /

6 Effects of laminarin on blood parameters of Epinephelus coioides LI Wen-wu 1, YIN Guang-wen 1, LIN Xi 1, HUANG Zhi-jian 1, LIN Jian-bin 2, JIANG He-ji 1, ZHU Qing-guo 2 (1. Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou , China; 2. Fujian Provincial Institute of Freshwater Fisheries, Fuzhou , China) Received: Apr., 28, 2014 Key words: laminarin; Epinephelus coioides; cytokines; blood biochemistry; non-specific immune Abstract: This study was conducted to evaluate the effects of laminarin on the immunological and biochemical parameters of Epinephelus coioides. 120 fish was randomly divided into four groups. The fish in blank control group was fed with basal diet, while the fish low, medium and high doses of laminarin groups was fed with the basal diet supplemented with 0.5%, 1.0%, and 1.5% laminarin, respectively, for 48 days. Then the cytokines and blood biochemical parameters (IL-2, IL-6, IFN-α, TP, UREA, CREA, ALP, AST, ALT, LZM, CAT and SOD) were investigated. The levels of cytokines ( IL-2, IL-6 and IFN-α) were higher than those in the control group, the levels of total protein and the activity of LZM, CAT and SOD were also higher than those in the control group. But the levels of C3, C4, UREA and CREA, and the activity of ALP were lower than those in the control group. There was no marked difference in the levels of ALT and AST in control and treated groups, suggesting that the supplementary feed had no adverse effect on the function of liver or kidney. These results suggest that laminarin can modulate the immune response of E. coioides. ( 本文编辑 : 谭雪静 ) 64 / 2015 / 39 / 6

instillation therapy combined with Western medicine can reduce the levels of ALT, TBA, ALP, TBIL, DBIL and GGT, and improve the anti-cmv-igm negative

instillation therapy combined with Western medicine can reduce the levels of ALT, TBA, ALP, TBIL, DBIL and GGT, and improve the anti-cmv-igm negative 在线投稿 : http://www.baishideng.com/wcjd/ch/index.aspx 帮助平台 : http://www.wjgnet.com/esps/helpdesk.aspx DOI: 10.11569/wcjd.v23.i28.4568 世界华人消化杂志 2015 年 10 月 8 日 ; 23(28): 4568-4573 ISSN 1009-3079