Microsoft Word - 4——胡志国_news_.doc

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1 3 个凡纳滨对虾引进群体对温度和盐度耐受力的配合力分析胡志国, 刘建勇, 袁瑞鹏, 张嘉晨 (, ) 摘要 : 为筛选凡纳滨对虾 (Litopenaeus vannamei) 人工选育亲本的最佳组合方式, 作者采用完全双列杂交交配设计, 对 3 个凡纳滨对虾引进群体建立了 9 个自繁和杂交组合群体, 研究了亲本和子代对温度和盐度的耐受力研究结果表明 : 9 个群体对温度盐度胁迫的耐受性均存在显著差异 (P<0.05), 耐高温性状中亲优势 (MP) 和超亲优势 (BP) 的变化范围是 17.71%~52.95% 和 30.07%~37.96%, 其中 UM 和 UH 群体的杂交中亲优势均值最高, 达到 38.61%; 耐低温性状 MP 和 BP 的变化范围是 22.04%~77.03% 和 33.93%~31.17%, 其中 UM 和 TZ 群体的杂交中亲优势均值最高, 为 60.85%; 耐高盐性状 MP 和 BP 的变化范围是 39.41%~76.96% 和 42.12%~19.07%, 其中 UM 和 TZ 群体的杂交中亲优势均值最高 ; 耐低盐性状 MP 和 BP 的变化范围是 44.89%~37.05% 和 45.68%~28.21%, 而 UM 和 TZ 群体的杂交中亲优势均值最低, 仅为 9.855%; 杂交后代耐受性的表现受到父本母本一般配合力以及杂交组合特殊配合力共同影响, 其中 UM 群体耐高低温性状的一般配合力较高, 而 TZ 群体耐高低盐性状的一般配合力较高 ; 特殊配合力分析表明, UM UH 为强优势组合, 存在较强的抗逆非加性效应, 杂交优势明显本研究从数量遗传水平评估选育群体对温盐的耐受性能, 可为进一步家系选育提供候选材料关键词 : 凡纳滨对虾 (Litopenaeus vannamei); 温度 ; 盐度 ; 杂种优势 ; 配合力中图分类号 : Q 文献标识码 : A 文章编号 : (2016) doi: /hykx (Litopenaeus vannamei),, [1], ( ),, [2], Griffing 1(),,, [3-4] Ponce-Palafox [5] ; Pan [6] ; [7], [8], 3, Griffing 1, 9 F1,,,,,, : ; : : (A201208B05); ( , ) [Foundation: Marine Fisheries Technology Program of Guangdong Province of China(No.A201208B05); Science Research and Technology Development Program of Guangxi Province of China(No , No )] : (1990-),,,,, @qq.com;,, liujy70@126.com Marine Sciences / Vol. 40, No. 1 /

2 1 材料和方法 1.1 实验材料 3 SIS, SIS, UM UH TZ ~6, ( ),, (Loligo chinensis) (Ostreae) (Nereis succinea),, 9, 1, ( ) 3~4 cm, 9 表 1 凡纳滨对虾 3 个引进群体的完全双列杂交 Tab. 1 Complete diallel cross of three populations of L. vannamei ( ) ( ) UM UH TZ UM UM UM UM UH UM TZ UH UH UM UH UH UH TZ TZ TZ UM TZ UH TZ TZ 1.2 耐受性实验设计 [9-10], 48 h, 48 h, ( ) (11 0.5) , 30, 14 cm 12 cm 12 cm, m 1.2 m 0.4 m 3 1 ( 48 h ), 7 12 h,,, [11],, 12 h 1,, 48 h, 1.3 理化因子的控制 48 h, : (1),, JY-H25A, 2500 W 3,, ±0.5 (2), MQK-SA2 ( 0.001) 1.4 数据处理 Excel 2007, SPSS 19.0, (P<0.05) Cruz [12], 2 : F P F P MP B 100, BP P0 PB : MP ; F 1 ; P 0 ; BP ; P B [13] : Yijk u Si Dj SDij Eijk Y ijk i j k, u, S i i, D j j, SD ij i j, E ijk, N(0, σ 2 e ) (GCA)(SCA) : (X X)b=X Y : Sˆ Dˆ SD ˆ SD ˆ SD ˆ 0 i j i j ij 2 结果与分析 2.1 急性胁迫的耐受力比较 9 F1 48 h 2 UK UK 48 h 26 / 2016 / 40 / 1

3 , 61.11%±2.22%, UM TZ 15.55%±4.01%; 9 48h 8.00%~75.00%, TZ UH, UH TZ ; 48 h 17.78%~ 73.34%; TZ UM 48 h, 16.67%±1.93%, UM UH TZ UH 48h, 80.00%;,, (P<0.05), 2.2 各理化因子耐受性的杂种优势 3 6 MP BP 17.71%~52.95% 30.07%~37.96%, UM UH, 38.61%; MP BP 22.04%~77.03% 33.93%~31.17%, UM TZ, 60.85%; MP BP 39.41%~76.96% 42.12%~19.07%, UM TZ ; MP BP 44.89%~37.05% 45.68%~ 28.21%, UM TZ, 9.855% 表 2 9 个凡纳滨对虾杂交组合群体在温度和盐度胁迫条件下的存活率 Tab. 2 Survival rate of different populations of L.vannamei under temperature and salinity intimidation 48 h (%) UM UH 52.22±6.76 ab 30.00±8.33 c 37.78±4.01 cd 80.00±3.33 a UM UM 30.00±3.33 c 41.67±11.67 c 21.11±4.01 e 48.89±8.68 de UM TZ 15.55±4.01 d 63.34±3.34 ab 73.34±3.33 a 66.67±2.09 bc UH UH 36.67±5.77 bc 15.00±1.67 d 46.67±6.67 bc 57.78±6.19 cd UH TZ 52.22±4.01 ab 75.00±8.33 a 17.78±6.76 e 68.89±2.22 b UH UM 61.11±2.22 a 20.00±3.33 d 55.55±1.28 a 43.33±6.94 e TZ UM 43.33±3.85 bc 46.67±3.34 bc 33.33±1.72 de 16.67±1.93 f TZ UH 33.33±8.39 c 8.00±1.67 d 57.78±7.29 ab 80.00±5.09 a TZ TZ 15.56±5.89 d 11.00±3.75 d 40.00±5.77 cd 46.67±1.93 de 37.78± ± ± ±4.39 : (P<0.05) 表 3 9 个凡纳滨对虾杂交组合群体温度和盐度耐受力的杂种优势 Tab. 3 Heterosis for temperature and salinity torlerance ability of different populations of L.vannamei (%) (%) UM UH UH UM UM TZ TZ UM UH TZ TZ UH Marine Sciences / Vol. 40, No. 1 /

4 2.3 配合力分析 4 :,, 5,, UM, ; TZ, , UM UH 表 4 凡纳滨对虾群体温度和盐度耐受力配合力的方差分析 Tab. 4 Variance analysis for combining ability for temperature and salinity torlerance ability of different populations of L.vannamei F * ** ** F ** ** ** F * * ** F ** : *(P<0.05), ** (P<0.01) 表 5 凡纳滨对虾群体温度和盐度耐受力配合力效应分析 Tab. 5 Combining ability effect analysis for temperature and salinity torlerance ability of different populations of L.vannamei UM UH TZ UM UH TZ UM UH TZ UM UH TZ UM UH UM TZ UH TZ 讨论 3.1 理化因子耐受性与选育的结合,, [7], 28 / 2016 / 40 / 1

5 , [14-18],, ;,, 3.2 耐受性比较与杂种优势分析 ( ), [19] [20],, 3,, %~52.95%, You [21],,,,, [22],,,,, [23] 3,, UH, ~0.2157,, UM UH,, UH,,,,,, (BLUP ),,,,,,, : [1]. - [J]., 1990, 14(3): Zhang Weiquan. Biological profile of important world aquaculture species-penaeus vannamei[j]. Marine Sciences, 1990, 14(3): [2],,,. [J]., 2012, 8(6): Huang Jianhua, Li Yong, Yang Qibin, et al. Comparison of tolerance to ammonia-n in Penaeus monodon families[j]. South China Fisheries Science, 2012, 8(6): [3],,,. [J]., 2012, 36(6): Sang Shitian, Yan Xiwu, Huo Zhongming, et al. The diallel cross between large and small sizes within family of Manila clam (Ruditapes philippinarum)[j]. Journal of Fisheries of China, 2012, 36(6): [4],,,. [J]., 2014, 33(2): Zhang Hui, Yan Xiwu, Zhang Yuehuan, et al. Diallel crosses between cow color strain and ocean-orange color strain of Manila clam Ruditapes philippinarum[j]. Fisheries Science, 2014, 33(2): [5] Ponce-Palafox J, Martinez-Palacios C A, Ross L G. The effects of salinity and temperature on the growth and survival rates of juvenile white shrimp, Penaeus vannamei, Boone, 1931[J]. Aquaculture, 1997, 157(1): [6] Pan L Q, Zhang L J, Liu H Y. Effects of salinity and ph on ion-transport enzyme activities, survival and growth of Litopenaeus vannamei postlarvae[j]. Aquaculture, 2007, 273(4): [7],,,. 10 [J]., 2010, 4: Marine Sciences / Vol. 40, No. 1 /

6 Lin Hongjun, Zhang Lüping, Shen Qi, et al. The comparison of tolerance to fresh water in 10 full-sib families of white leg shrimp, Litopenaeus vannamei[j]. Transactions of Oceanology and Limnology, 2010, 4: [8] Davis D A, Boyd C E, Rouse D B, et al. Effects of potassium, magnesium and age on growth and survival of Litopenaeus vannamei post-larvae reared in inland low salinity well waters in West Alabama[J]. Journal of the World Aquaculture Society, 2005, 36(3): [9] Magallón Barajas F J, Servín Villegas R, Portillo Clark G, et al. Litopenaeus vannamei (Boone) post-larval survival related to age, temperature, ph and ammonium concentration[j]. Aquaculture Research, 2006, 37(5): [10] Zhang P, Zhang X, Li J, et al. The effects of body weight, temperature, salinity, ph, light intensity and feeding condition on lethal DO levels of whiteleg shrimp, Litopenaeus vannamei (Boone, 1931)[J]. Aquaculture, 2006, 256(1): [11] Li E, Chen L, Zeng C, et al. Growth, body composition, respiration and ambient ammonia nitrogen tolerance of the juvenile white shrimp, Litopenaeus vannamei, at different salinities[j]. Aquaculture, 2007, 265(1): [12] Cruz P, Ibarra A M. Larval growth and survival of two catarina scallop(argopecten circularis, Sowerby, 1835) populations and their reciprocal crosses[j]. Journal of Experimental Marine Biology and Ecology, 1997, 212(1): [13],,,. [J]., 2009, 16(2): Wang Bingqian, Gu Wei, Gao Huijiang, et al. Prediction of hybridization advantage among five strains of rainbow trout by combination ability and SSR markers[j]. Journal of Fishery Sciences of China, 2009, 16(2): [14],,,. [J]., 2001, 20(3): Li Runyin, Chen Jiekang, Jiang Hongliang, et al. Experiments on temperature suitable for postlarva of Penaeus japonicus[j]. Fisheries Science, 2001, 20(3): [15],. [J]., 1997, 4(3): Pan Luqing, Wang Kexing. Effects of temperature on growth development and digestive enzyme activities of the larvae of Penaeus chinensis[j]. Journal of Fishery Sciences of China, 1997, 4(3): [16],. [J]., 2002, 17(3): Zhang Shuo, Dong Shuanglin. The effects of food and salinity on energy budget of juvenile shrimp of Penaeus chinensis[j]. Journal of Dalian Fisheries University, 2002, 17(3): [17], 甡,. [J]. ( ), 2005, 35(1): Wang Xingqiang, Ma Shen, Dong Shuanglin. Effects of salinity and dietary protein levels on survival, growth and energy conversion of juvenile Litopenaeus vannamei[j]. Journal of Ocean University of China, 2005, 35(1): [18],,. [J]., 2004, 11(6): Jiang Lingxu, Pan Luqing, Xiao Guoqiang. Effects of ammonia-n on immune parameters of white shrimp Litopenaeus vannamei[j]. Journal of Fishery Sciences of China, 2004, 11(6): [19] Deng Y W, Liu X, Zhang G F, et al. Heterosis and combining ability: a diallel cross of three geographically isolated populations of Pacific abalone Haliotis discus hannai Ino[J]. Chinese Journal of Oceanology and Limnology, 2010, 28(6): [20],,,. 4 [J]., 2009, 40(1): Sun Chengbo, Chen Guoliang, Tong Hanrong, et al. Morphological differences of four imported Litopenaeus vannamei groups from the United States[J]. Oceanologia Et Limnologia Sinica, 2009, 40(1): [21] You W W, Ke C H, Luo X, et al. Growth and survival of three small abalone Haliotis diversicolor populations and their reciprocal crosses[j]. Aquaculture Research, 2009, 40(13): [22] Pillai B R, Mahapatra K D, Ponzoni R W, et al. Genetic evaluation of a complete diallel cross involving three populations of freshwater prawn(macrobrachium rosenbergii) from different geographical regions of India[J]. Aquaculture, 2011, 319(3): [23],,,. [J]., 2014, 10(1): Hu Zhiguo, Liu Jianyong, Bao Xiufeng, et al. Combining ability and heterosis of hybridization between cultured and wild stocks of Haliotis diversicolor supertexta[j]. South China Fisheries Science, 2014, 10(1): / 2016 / 40 / 1

7 Analysis of combining ability of survival of imported Litopenaeus vannamei populations under temperature and salinity stress HU Zhi-guo, LIU Jian-yong, YUAN Rui-peng, ZHANG Jia-chen (Fisheries College, Guangdong Ocean University, Zhanjiang , China) Received: Oct., 9, 2014 Key words: Litopenaeus vannamei; temperature; salinity; heterosis; combining ability Abstract: To select the optimal combination of parents for artificial breeding, three introduced populations of Litopenaeus vannamei were used to establish nine inbred and hybridized populations by complete diallel crosses, and their temperature and salinity tolerance were compared. Significant differences in temperature and salinity tolerance were observed among the nine populations. Mid-parent heterosis and heterobeltiosis for tolerance to high temperature were 17.71% 52.95% and 30.07% 37.96%, respectively, in which the UM and UH groups had the highest mid-parent heterosis (38.61%). Mid-parent heterosis and heterobeltiosis for tolerance to low temperature were 22.04% 77.03% and 33.93% 31.17%, respectively, in which the UM and TZ groups had the highest mid-parent heterosis (60.85%). Mid-parent heterosis and heterobeltiosis for tolerance to high salinity were 39.41% 76.96% and 42.12% 19.07%, respectively, in which the UM and TZ groups had the highest mid-parent heterosis (60.85%). Mid-parent heterosis and heterobeltiosis for tolerance to low salinity were 44.89% 37.05% and 45.68% 28.21%, respectively, in which the UM and TZ group had the lowest mid-parent heterosis ( 9.855%). The general combining ability of parents and special combining ability of hybrid combinations influenced the tolerance performance of the hybrid offsprings; of these, the UM group had the highest general combining ability for resistance traits of high and low temperature, while the TZ group had the highest general combining ability for resistance traits of high and low salinity. The specific combining ability analysis showed that UM UH, with a stronger, non-additive effect and hybrid superiority, was a strong hybrid combination and could be used as a candidate material for further family selection. ( 本文编辑 : 谭雪静 ) Marine Sciences / Vol. 40, No. 1 /

Microsoft Word 胡志国_new_.doc

Microsoft Word 胡志国_new_.doc 46 3 Vol.46, No.3 2015 5 OCEANOLOGIA ET LIMNOLOGIA SINICA May, 2015 (Litopenaeus vannamei) G 0 * ( 524088) (Litopenaeus vannamei)7, 11, 270 (), Kung,, 11 270 13.52cm 31.05g, (P