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1 36 6 Vol.36, No JOURNAL OF FISHERIES OF CHINA June, 2012 文章编号 : (2012) DOI: /SP.J 团头鲂 3 个地理种群杂交效果的配合力和微卫星标记预测 1, 1, 1, 1, 1, 1, 2, 1* (1.,,, ; 2., ) : 12 (SSR) 3, 3,, F 1,, ;,,,, 3 3, (0.848), (0.820); ; 3 ( ), ( ), : ; ; ; ; : Q ; Q ; S 917 : A (Megalobrama amblycephala Yih),, (Cypriniformes), (Cyprinidae), (Megalobrama),,, 20 60, [1],,, (combining ability), [2],, [3] [4] [5] ()(Macrobrachium rosenbergii) (Paralichthys olivaceus) 3 (general combining ability, GCA) 收稿日期 : 修回日期 : 资助项目 : (CARS-46-05); (2011PY023); ; (2011SC27C) 通讯作者 :, wangwm@mail.hzau.edu.cn

1; S 917 : A (Megalobrama amblycephala Yih),, (Cypriniformes), (Cyprinidae), (Megalobrama),,, 20 60, [1],,, (combining ability), [2],, [3] [4] [5] ()(Macrobrachium rosenbergii)

2 (specific combining ability, SCA),, , (L) (P) (Y) g ( 1) 5,, (4.0 m 3.0 m 1.5 m),, 400, 8:00 17:00 表 1 不同地理种群亲本搜集点和数量 Tab. 1 Location and sampling information of three geographical populations of M. amblycephala population, Y, L, P location longitude latitude individuals total E N E N E N 数据收集 6, 4, 2 50 Microsoft Excel SAS 9.01 杂交试验数据统计分析, 6,, [6-7] : y ijk = μ + s i + d j + r ij + e ijk, y ijk i j k ; e ijk ; μ ; s i i ( ); d j j ( ); r ij i j () SSR 标记数据分析, DNA PCR [8] [9] GenBank 8 Genome-SSR 4 EST-SSR puc18 /Msp POPGEN 32 3 (P) (H e ) Nei s (D) [10] ; Microsoft Excel (PIC)(MI), [11-12] n n 1 n 2 PIC = 1 p 2 2 i 2 pi pj i= 1 i= 1 j= i+ 1, p i p j i j, n ; MI = N PIC, N, PIC , (L) (Y), ( 3); ;, (P), (Y) 2.2 配合力方差分析 ( 2), (P<0.01), (GCA), (SCA) ( 4),, 一般配合力和特殊配合力分析 F 1

amblycephala population, Y, L, P location longitude latitude individuals total E152 10 N29 85 12 15 27 E114 51 N30 34 15 16 31 E115 95 N29 24 15 20 35 1.2 数据收集 6, 4, 2 50 Microsoft Excel SAS 9.

3 6, : 表 2 3 个地理种群团头鲂间杂交子代 6 月龄全长和体质量比较 Tab. 2 Comparison of total length and body weight of hybrids among three geographical populations of M. amblycephala combination / mm total length / g body weight L L ± 9.40 bc 2.68 ± 2.24 c L P ± c 2.07 ± 1.77 c L Y ± ab 3.81 ± 3.30 a P L ± b 3.12 ± 2.49 b P P ± c 2.37 ± 2.26 c P Y ± c 2.27 ± 2.32 c Y L ± c 2.15 ± 2.44 c Y P ± c 2.37 ± 2.43 c Y Y ± 7.77 a 3.36 ± 1.61 b : (P<0.05), (P>0.05) Notes: The different subscripts show significant difference in the same row (P<0.05), and the same subscripts demonstrate insignificant difference (P>0.05). 表 3 3 个地理种群间的亲本配合力方差分析 Tab. 3 ANOVA analysis about body weight and length of hybrids among three geographical populations M. amblycephala MS variation df total length body weight sire ** ** dam ** ** sire dam ** ** error ** 3.58 ** : ** (P<0.01) Notes: ** means significant difference (P<0.01). 表 4 3 个地理种群团头鲂的配合力方差来源分析 Tab. 4 Analysis of variation source in total length and body weight of M. amblycephala source total length body weight GCA GCA SCA () error total combination ability F 1, [13], 5,,,,,, 表 5 团头鲂亲本全长和体质量的配合力相对效应值 Tab.5 General combining ability relative effect and specific combining ability relative effect among three populations of M. amblycephala full length SCA GCA SCA GCA SCA body weight GCA SCA GCA %

49 b P P 49.39 ± 12.36 c 2.37 ± 2.26 c P Y 46.78 ± 14.73 c 2.27 ± 2.32 c Y L 46.16 ± 14.32 c 2.15 ± 2.44 c Y P 48.97 ± 13.42 c 2.37 ± 2.43 c Y Y 57.96 ± 7.77 a 3.36 ± 1.61 b : (P<0.05), (P>0.

4 SSR 微卫星位点的多态信息含量 (PIC) 有效等位基因数 (N e ) 近交系数 (F IS ) 期望杂合度 (H e ) 和标记索引值 (MI) 85 SSR, 12 ( 6), , 14.75, 5~ , (0.848), (0.820) , 8.959~ , (0.005), [14] 群体间的遗传距离 ( ), ( ), 3 ( 7), 3, [15] SRAP [16],,, 4 EST-SSR 表 6 各标记座位在 3 个地理种群团头鲂的平均多态信息含量有效等位基因数近交系数和期望杂合度 Tab. 6 Polymorphism information content, numbers of effective allele, F IS and expected heterozygosity of different marker loci in three populations of M. amblycephala locus PIC N e F IS H e MI PIC N e F IS H e MI PIC N e F IS H e MI TTF TTF TTF TTF TTF TTF TTF TTF SSR SSR SSR SSR mean 表 7 3 个地理种群团头鲂的遗传距离和相似系数 Tab. 7 Genetic distance and similarity among three geographical populations of M. amblycephala :, Notes: Figures below the diagonal show genetic distance, the above expresses genetic similarity [5],,, 3,,,,

6 Polymorphism information content, numbers of effective allele, F IS and expected heterozygosity of different marker loci in three populations of M.

5 6, : [17], 2,, F 1,,,,, 6, 3.2, (PIC)(N e ) (MI) [18] SSR 3,, , [7] 12, PIC, ; [19], [20],,, [21], ~ , ; ~ , ; ~ ,, ~ , 3.3, [22],, DNA (SSR) (),,,,, : [1]. [J]., 2009(4): [2]. [M]. :, [3],,,. SRAP [J]., 2006, 13(4): [4],,,. GHR Promoter [J]. :, 2008, 38(5): [5],,,. [J]., 2009, 16(2): [6]. () SAS [J]., 2001, 22(4): [7]. [M]. :, [8]. [M]. :, [9] Shou T, Si L, Wan C. Development of microsatellite markers for blunt snout bream Megalobrama amblycephala using 5 -anchored PCR[J]. Permanent Genetic Resources Note, 2008, 9(3): [10] Nei M. Genetic distance between populations [J]. American Naturalist, 1972, 106: [11] Botstein D, White R L, Skolnick M, et al. Construction of genetic linkage map in man using restriction fragment length polymorphisms [J]. American Journal of Human Genetics, 1980, 32: [12] Smith J S C, Chin E C L, Shu H, et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): Comparison with data from RFLPs and pedigree[j]. Theoretical and Applied Genetics, 1997, 95: [13],,,. [J]., 2010, 34(1):

[6]. () SAS [J]., 2001, 22(4): 72 77. [7]. [M]. :, 1997. [8]. [M]. :, 1999. [9] Shou T, Si L, Wan C.

6 [14] Weir B S, Cockerham C C. Estimation F-statistics for the analysis of population structure [J]. Evolution, 1984, 38: [15],,,. SRAP 3 [J]., 2010, 29(5): [16],,,. [J]., 2012, 31(1): 1 5. [17]. [M]. :, [18],,,. SSR [J]., 2005, 21(2): [19],,,. [J]., 2000, 27(7): [20],,,. [J]., 2008, 29(3): [21],. [J]., 2010, 26(15): [22]. [M]. :, Prediction of hybrid vigor among three geographical populations of Megalobrama amblycephala by combination ability and SSR markers ZENG Cong 1, ZHANG Yao 1, CAO Xiao-juan 1, LUO Wei 1, LIU Xiao-lian 1, GAO Ze-xia 1, QIAN Xue-qiao 2, WANG Wei-min 1* (1. College of Fishery, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education & Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan ; 2. Animal Husbandry and Fisheries Research Center of Haid Group Co, Ltd, Guangzhou , China) Abstract: Hybrid vigor among three geographical populations of Megalobrama amblycephala, including population (L), population (P) and population (Y), was predicted by combination ability and SSR markers. The first generation individuals of 9 complete diallel cross combinations of three populations were measured with total length and body weight. The analysis of combination ability showed that the sire of Yuni population had the largest general combining ability effective value; however, the largest general combining of total length in dam was Poyang population, while that of body weight was Liangzi population. There were high effective combinations among P P, L Y and Y Y which had high specific combining ability, so these combinations were suitable as basic selective populations. The results obtained from SSR markers showed that all three populations had high genetic heterogeneity (H e, ), polymorphism information content (PIC, ) and marker index (MI, ). Among the three populations, Liangzi population and Yuni population revealed the largest genetic distance, reaching According to the result, L Y would have the highest hybrid vigor among those combinations. Key words: Megalobrama amblycephala; geographical population; combination ability; simple sequence marker; hybrid vigor prediction Corresponding author: WANG Wei-min. wangwm@mail.hzau.edu.cn

Prediction of hybrid vigor among three geographical populations of Megalobrama amblycephala by combination ability and SSR markers ZENG Cong 1, ZHANG Yao 1, CAO Xiao-juan 1, LUO Wei 1, LIU Xiao-lian

2 第 27 卷京七 W557 合 344 杂 1 表 1 名称及来源 Table 1 Name and source of inbred lines 来源唐四平头 K10 唐四平头 B14 兰卡斯特 H 旅 28 兰卡斯特丹 340 来源 Reid Re

2 第 27 卷京七 W557 合 344 杂 1 表 1 名称及来源 Table 1 Name and source of inbred lines 来源唐四平头 K10 唐四平头 B14 兰卡斯特 H 旅 28 兰卡斯特丹 340 来源 Reid Re 第 27 卷第 4 期 2015 年 8 月 Journal of Heilongjiang Bayi Agricultural University 27(4):1~5 Aug. 2015 doi:10.3969/j.issn.1002-2090.2015.04.001 外引加拿大玉米的配合力分析和应用张玉胡 1,2, 高树仁 1, 王霞 1, 孙丽