Microsoft Word - 8--菅玉霞_news_.doc

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1 温度盐度对斑点鳟发眼卵孵化的影响菅玉霞, 潘雷, 胡发文, 高凤祥, 张少春, 王雪, 李莉, 郭文 (, ) 摘要 : 为了确定斑点鳟 (Oncorhynchus mykiss) 发眼卵孵化的适宜温度盐度条件, 作者通过设置不同的孵化温度和盐度, 研究了温度盐度对斑点鳟发眼卵孵化的影响结果表明, 发眼卵孵化的适宜温度范围为 8~16, 孵化率都在 (73.33±2.04)% 以上, 最适温度为 10, 孵化率达 (87.77±1.23)% 温度高于 24 时发眼卵不能孵化温度在 6~22 内, 斑点鳟发眼卵孵化时间与温度成负相关关系斑点鳟胚胎发育的平均积温值为 343 d 发眼卵孵化的适宜盐度范围为 0~15, 孵化率都在 (85.33±1.92)% 以上, 最适盐度为 0, 孵化率达 (97±1.13)% 盐度高于 15 时的孵化率为 0 盐度在 0~15 内, 发眼卵孵化时间与盐度成正相关关系关键词 : 斑点鳟 (Oncorhynchus mykiss); 发眼卵 ; 温度 ; 盐度 ; 孵化中图分类号 : S965.2 文献标识码 : A 文章编号 : (2014) doi: /hykx (Oncorhynchus mykiss),, (Salmonidae),,, (Oncorhynchus mykiss) (Oncorhynchus mykiss) (Oncorhynchus mykiss), 2010 [1-3], (Oncorhynchus keta), 0~24, 8~21, 10~18 ;, 0~33 [4], [5-21], [1-4],,, 1 材料与方法 1.1 材料, , 245 d, 24.5 d ml 1.2 方法 : ml 60,, 3 67cm 46 cm 35 cm, WEIPRO MX300 IC, ±0.5, 1 /h : ; ; ( ) : ; : : ; ( hy) : (1979-),,,,,, : ;,,, : , yzszsjd@126.com Marine Sciences / Vol. 38, No. 9 /

( ± ) 2 000 ml 60, 3 (10±0.5) 67 cm 46 cm 35 cm, WEIPRO MX300 IC, 10 10, ; 10,, 22 24,, 10 2 1.3 数据处理 Spss19.0, (ANOVA) Duncan, Microsoft Excel 2003 2 结果与分析 2.1 温度对发眼卵孵化的影响 2.1.1 1, (P<0.

2 ( ± ) ml 60, 3 (10±0.5) 67 cm 46 cm 35 cm, WEIPRO MX300 IC, 10 10, ; 10,, 22 24,, 数据处理 Spss19.0, (ANOVA) Duncan, Microsoft Excel 结果与分析 2.1 温度对发眼卵孵化的影响 , (P<0.05) 1, 6~22, 1 Fig.1 Hatching rate of eyed eggs of Oncorhynchus mykiss under different water temperature (P<0.05), Figure in the same letters without significant difference(p<0.05),the same below Fig.2 2 Hatching of eyed eggs of O. mykiss A. 1; B. 2; C. A. the incubation of eyed eggs 1; B. the incubation of eyed eggs 2; C. the newly hatched larvae , 6~22, 6 15 d, 22, 4d N(d) T( ), : N= ln(t) (r 2 =0.9924) 2.1.3,, [22] 1, 3 Fig.3 Effect of temperature on hatching days of eyed eggs of O. mykiss 333~350 d, 343 d 48 / 2014 / 38 / 9

3 表 1 斑点鳟发眼卵发育与水温的关系 Tab.1 Relationship between development of eyed eggs of Oncorhynchus mykiss and water temperature ( ) (d) ( d) ( d) ( d) 盐度对发眼卵孵化的影响 , (P<0.05), 20, 30, 10 h, 20 h 0~15, 0, 97% 4 (10±0.5) Fig.4 Hatching rate of eyed eggs of O. mykiss under different salinity(10±0.5) , 15 5, 0~15, 0 11 d, 10~15 13d N(d) S( ), : N=11.023S (R 2 =0.9604) 3 讨论与结论 3.1 温度对发眼卵孵化的影响 5 Fig.5 Effect of salinity on hatching days of eyed eggs of O. mykiss,, d, d, d [23] 8~10 (340~360) d [24] (O. masou) [25] d, 8.4, 252 d (Salvelinus leucomaenis) [15] d ; (Salmo salar) 8~ [26],,, d, d, d [27], d, d, d,, d, d, Marine Sciences / Vol. 38, No. 9 /

4 22, 28.5d, [28], 7.5, 46 d, 9, 30~38 d, 12, 26 d ; 8 60 d, ( 4 ) 90 d [29] (Hucho taimen Pallas) [30] 8.3~11.6 时, 38~41 d 3~11 24 d, 2~8 45 d [31] : [10] [32] [33],,,,,,, 18,,, [34], 10, 18~22,, 24,, 8~16, 盐度对发眼卵孵化的影响,, (Verasper moseri) [5] (Cynoglossus semilaevis) [8] (Oplegnathus fasciatus) [10],,,,,, [35],,, 20, 30, 10 h, 20 h, 0~15,,, 0~15, 0, : [1],,,. [J]., 2011, 28(5): [2],,,. [J]., 2012, 34(3): [3],,,. [J]., 2012, 33(7): [4],,,. [J]., 2012, 40(3): [5],,,. [J]., 2008, 29(6): [6],,,. ( ) ( ) [J]., 2007, 28(3): [7]. 鲿 [J]., 2012, 36(11): [8],,,. [J]., 2006, 24(3): [9],,,. [J]., 2012, 36(7): [10],,,. [J]. 50 / 2014 / 38 / 9

5 , 2009, 30(3): [11],,. [J]., 2011, 7(5): [12],,,. [J]., 2005, 9 (4): [13],,,. [J]., 2001, 14(2) : [14],,. [J]., 2006, 30(3): [15],,,. [J]., 2010, 45(5) : [16] Mihelakakis A, Kitajima C Effects of salinity and temperature on incubation period, hatching rate, and morphogenesis of the silver sea bream, Sparus sarba[j] Aquaculture, 1994, 126(3-4): [17] Morgan R P, Rasin V J. Influence of temperature and salinity on development of white perch eggs[j]. Transactions of the American Fisheries Society, 1982, 111(3): [18] Yasuhisa K, Tsuzumi M. Effects of salinity on the embryonicdevelopment and larval survival activity index of red spotted grouper Epinephelus akaara [J]. Saibai Giken, 1993, 22(1): [19] Turner M A, Viant M R, Teh S J, et al. Developmental rates, structural asymmetry, and metabolic fingerprints of steelhead trout (Oncorhynchus mykiss) eggs incubated at two temperatures[j]. Fish Physiology Biochemistry, 2007, 33: [20] Svecevicius G. Avoidance response of rainbow trout Oncorhynchus mykiss to heavy metal model mixture after long-term exposure in early development[j].bull Environ Contam Toxicol, 2003, 71: [21] Lazorchak J M, Smith M E. Rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis) 7-day survival and growth test method[ J]. Arch Environ Contam Toxicol, 2007, 53: [22],. [J]., 2008, 36(7): [23],,,. [J]., 2005, 9(4) : [24],,. [J]., 2009, 4: [25],,,. [J]., 2000, 13(2): 1-5. [26],,. [J]., 2004, 11: [27], [M]. :, 2001 [28],, 鲀 [J]., 2004, 34(2): 6-8. [29],, [M]. :, 2008 [30],,,. [J]., 2008, 17(4): [31],,,. [J]., 2002, 15(1): 1-4. [32]. [J]., 1965, 7(3): [33],. [J]., 2002, 53(1) : [34],, [J], 1991, 15: [35], [J]., 1998, 22(4): Marine Sciences / Vol. 38, No. 9 /

6 Effects of temperature and salinity on the hatching of eyed eggs of Oncorhynchus mykiss JIAN Yu-xia, PAN Lei, HU Fa-wen, GAO Feng-xiang, ZHANG Shao-chun, WANG Xue, LI Li, GUO Wen (Ocean and Biology Institute of Shandong Province, Qingdao, , China) Received: May, 17, 2013 Key words: Oncorhynchus mykiss; eyed eggs; temperature; salinity;hatch Abstract: In order to determine the suitable temperature and salinity for eyed eggs of Oncorhynchus mykiss, we studied the effect of temperature and salinity on the hatching of eyed eggs by setting different temperatures and salinities. The results showed that the suitable temperature range for hatching was 8~16 with hatching rate higer than (73.33±2.04)%. The most suitable temperature was 10 with a high hatching rate of (87.77±1.23)%. The eyed eggs didn t hatch when the temperature was higher than 24. In the temperature range of 6~22, there was a negative correlation between the hatching time and temperature. The average accumulated temperature for embryo development was 343 d. The suitable salinity range for hatching was 0~15 with hatching rate higher than (85.33±1.92)%. The most suitable salinity was 0 with a high hatching rate of (97±1.13)% The hatching rate was 0 when the salinity was more than 15. In the salinity range of 0~15, there was a positive correlation between the hatching time and salinity. ( 本文编辑 : 谭雪静 ) 52 / 2014 / 38 / 9

Microsoft Word - 22 栗志民.doc

Microsoft Word - 22 栗志民.doc 44 6 Vol.44, No.6 2013 11 OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2013 (Argopecten irradians concentricus) * 1 1 2 2 (1. 524025; 2. 524022) 2011 10 2012 4 (Argopecten irradians concentricus),,,,,,,, (P