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1 6 1 Vol. 6 No Journal of Food Safety and Quality Jan., 2015 赵东伟 1, 陈世豪 1,2, 施祖灏 1, 陈大伟 1, 张云山 1,2, 陆俊贤 1, 高玉时 1*, 王杏龙 (1., ; 2., ) 2 摘要 : 目的 方法 15 d, 0.5 ml d 3 d 7 d 14 d 21 d 28 d 35 d 42 5, 结果 d3,, d 7, mg/kg, FAO WHO 20 mg/kg 结论 d 3~7, ;, 关键词 : ; ; ; ; Research on remove rule of white oil adjuvant n-alkanes residual in the chicken ZHAO Dong-Wei 1, CHEN Shi-Hao 1, 2, SHI Zu-Hao 1, CHEN Da-Wei 1, ZHANG Yun-Shan 1, 2, LU Jun-Xian 1, GAO Yu-Shi 1*, WANG Xing-Long 2 (1. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou , China; 2. College of Animal Science and Technology, Yangzhou University, Yangzhou , China) ABSTRACT: Objective To research the remove rule of residual of white oil adjuvant n-alkanes in the chicken. Methods The Suqin yellow chickens of 15 d-old were injected 0.5 ml white oil to right chest muscle. After injection, the samples of chest muscle of 5 tested chicken were taken and residue analysis of white oil at d 3, d 7, d 14, d 21, d 28, d 35 and d 42 were studied. Results The n-alkanes residual is of the highest at d 3, concentrations of n-alkanes decline gradually as the change of time. At d 7, white oil dropped to mg/kg, having conformed to the rule that white oil added in the food additives should not exceed 20 mg/kg, established by FAO and WHO. Conclusion Within 3~7 d, n-alkanes residue declined faster, especially short chain alkanes decreasing quantity and speed reached the highest value in this period. Compared with the short chain alkanes, the long chain alkanes eliminated and migrated relatively slower, needing more time to be metabolized completely in the chicken. KEY WORDS: white oil; n-alkanes; chicken; remove rule; metabolism 基金项目 : (2012BAD39B04-3) (2014GJFP ) (yz ) Fund: Supported by National Key Technology R&D Program (2012BAD39B04-3), National Poultry Product Quality Safety Risk Assessment Project(2014GJFP ) and Yangzhou Scientific and Technological Program (yz ) * 通讯作者 :,, gaoys100@sina.com *Corresponding author: GAO Yu-Shi, Researcher, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou , China. gaoys100@sina.com

2 1, : 引言 (white oil),,,, C 16 -C 31, [1],,,, [2],,, [3-5],,,,,,,,,,,, [6], [7,8] 2009, (ESFA), 12 mg/kg bw [9] FAO WHO, 20 mg/kg [10],,,, 2 材料与方法 2.1 仪器 - : Agilent6890A, Agilent ; : Agilent DB-1ms (60.0 m 0.32 mm 0.25 μm), J&W ; : 3-30 K, Sigma ; : HS501, IKA ; : N-EVA, Organomation Associates ; : HH-S4, ; : AB104-L( 0.1 mg), METTLER TOLEDO 2.2 药品及试剂 : ; : 500 ng/μl(c 10 -C 35 ), Dr. Ehrenstorfer ; :, SIGMA ; :, CNW ; :, Merck ; :, CNW ; :, 2.3 试验设计及饲养管理,,,, d, 0.5 ml d 3 d 7 d 14 d 21 d 28 d 35 d 42 5,,, 样品前处理 正烷烃的提取 2.00±0.01 g 50 ml, 5 ml 30 min, ; 5 ml, 15 ml,, 0.5 ml 尿素络合 4 ml, 4 (>12 h); 2 3 ml,, ; 2 ml 5%1 ml, 100, 10 ml, 2 ml ;, 1 ml, GC-MS 2.5 色谱条件 气相色谱条件 : Agilent DB-1 ms (60.0 m 0.32 mm 0.25 μm); :, %, 1 ml/min,, 1 μl; : 280 ; : 质谱条件 : 280 ;

3 186 6 : 230 ; : 150 ; : 6.5 min; : (EI), 70 ev; : /; (SCAN), (m/z) ; (SIM), C 16 -C 24 (m/z) : ( /min) 表 1 升温程序 Table 1 Oven program ( ) (min) (min) 结果, d 3 d 7 d 14 d 21 d 28 d 35 d 42 9 (C 16 C 17 C 18 C 19 C 20 C 21 C 22 C 23 C 24 ) (n-alkanes) 2, d , 7, d 3,,, d 42,,, 3 d, mg/kg, d 7, mg/kg,, d mg/kg d 3 d 7, ; d 7 d 14, ; d 14,, 4 讨论 2,, d 3 7, d 3 d 7,, d 7 d 14,,, d 3,, d 7 [10] d 20,, Fig Select ion chromatogram of n-alkanes residues of d 21 chicken sample

4 1, : 187 Table 2 表 2 肉鸡胸肌中正烷烃残留量 (n=5) Residues of n-alkanes in broiler breast muscle (n=5) (mg/kg) 3d 7d 14d 21d 28d 35d 42d n-c ± ± ± ± ± ±0.04 ND * n-c ± ± ± ± ± ±0.11 ND * n-c ± ± ± ± ± ± ±0.09 n-c ± ± ± ± ± ±0.09 ND * n-c ± ± ± ± ± ± ±0.02 n-c ± ± ± ± ± ± ±0.05 n-c ± ± ± ± ± ± ±0.05 n-c ± ± ± ± ± ± ±0.08 n-c ± ± ± ± ± ± ±0.09 n-alkanes 30.58± ± ± ± ± ± ±0.11 : ND-, [12],,,, ;,,,,, d 14, 3 ;,,, [13] Wilner [14] 9 11 w/o,,,, C 24, C 16 ~C 20,,,, C 16 ~C 31,,, [15],,, 3 d, mg/kg, d 7, mg/kg, d 7, FAO WHO 20 mg/kg 5 小结 42 d,, 3 7 d, ;,, 42 d,, 参考文献 [1]. [J]., 2003, 18(4): 1 6. Li LQ. White oil and technology [J]. Lubrication oil, 2003, 18(4): 1 6. [2],. [J]., 2004, 38(1): Pan HJ, Sun HX. Research progress of immunologic adjuvant [J]. Chin J Vet Drug, 2004, 38(1): [3],,. GC-MS [J]., 2011, 26(4):

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