21 13 1 2 1* 2 1. 430065 2. 445000 20 10 5 g kg - 1 5 mg kg - 1 ig 10 d ig HE 1 TFF1 TFF1 P < 0. 01 P < 0. 01 P < 0. 01 P < 0. 05 R285. 5 doi 10. 13422 /j. cnki. syfjx. 2015130100 A 1005-9903 2015 13-0100-05 Effect of Atractylodis Rhizoma Extract on Structure of Gastric Mucosa and Gastrointestinal Function in Rats with Spleen Deficiency LIU Fen 1 2 LIU Yan-ju 1* TIAN Chun-man 2 1. Hubei University of Chinese Medicine Wuhan 430065 China 2. Hubei Institute for Nationalities Enshi 445000 China Abstract Objective To observe the role of Atractylodis Rhizoma extract on protecting gastric mucosa and gastrointestinal dysfunction of rats with spleen deficiency syndrome and to elucidate the mechanism of Atractylodis Rhizoma extract regulating spleen deficiency syndrome. Method Male rats were fed with Xiaochengqi decoction and hunger to establish spleen deficiency syndrome models. Then the animal were randomly divided into model group Atractylodes Rhizome extract high middle and low dose group 20 10 5 g kg - 1 Domperidone group 5 mg kg - 1. After 10 days of treatment charcoal gavage method was used o determine residual rate of stomach and small intestinal propulsion ratio of the rats hematoxylin and eosin HE staining was used to observe the gastric mucosa pathological and histological changes like gastric mucosa tissue cells of glandular stomach area and ultrastructural alterations of intercellular junctions with transmission electron microscopy method laser Doppler blood flow meter was used to measure gastric mucosal blood flow immunohistochemical was used to determinate the expression of trefoil factor 1 TFF1 in gastric mucosa and expression in colon tissue volume. Result Compared with the normal group gastric mucosal morphology had significantly abnormal change in model group gastric mucosal blood flow gastric mucosa TFF1 and colon tissue volume expression were decreased P < 0. 01 residual rate of stomach was increased and small intestinal propulsion ratio was decreased P < 0. 05 P < 0. 01. After intervention of Atractylodis Rhizoma extract those indicators were improved P < 0. 05 P < 0. 01. Conclusion Atractylodis Rhizoma extract can inhibit the gastric mucosal damage of spleen deficiency rat protect and repair the damaged mucous membrane organization and improve the gastrointestinal function of spleen deficiency rats. Key words Atractylodes Rhizome extract spleen deficiency syndrome gastrointestinal function signal transduction 20141012 003 81073051 201007012-2 Tel 13972405805 E-mail 1183794354@ qq. com * Tel 027-68890239 E-mail lyj1965955@ sohu. com 100
21 13 D- 1 2. 2 10 / 2 10 ig ig 10 d 1. 0 g kg - 1 1 /2 2 β- 5 g kg - 1 20 g kg - 1 5. 0 mg kg - 1 ig 2. 3 2. 3. 1 1 ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30 1. 1 SD 60 min 30 mg kg - 1 ip 200 ± 20 g SCXK 2013-0105 0. 5 cm 1. 2 Atractylodis japonicae 46. 97% 17. 87% β- 12. 21% 130711 mv 20130610 2. 3. 2 CMC-Na 1 TFF1 Cajal DF130203 DF130401 = 4 / 100% 1. 3 UN-500 DG5032 H-600 XSP-300 Image-Pro Plus 5. 1 Motic - / 100% 2. 3. 3 2 2. 1 0. 5 cm 0. 5 cm 10% 5 μm 3 HE 4 5 3 1 60 g kg - 1 1 2. 3. 4 0. 1 cm 0. 1 cm 1 2. 5% 0. 1 mol L - 1 3 1% 101
21 13 0. 1 mol L - 1 3 1 Table 1 50% 70% 80% 95% 100% 2 15 min 2 15 min EPON812 1 1 30 min 1 /g kg h 37 24 h 60 48 h - 1 2. 3. 5 TFF1 IA 2. 4 x 珋 ± s SPSS 17. 0 x 珋 ± s n = 10 Influence of Atractylodes Rhizoma extract on gastric mucosal blood flow of model rat x 珋 ± s n = 10 P < O. 05 3 3. 1 P < 0. 01 P < 0. 05 P < 0. 01 1 GMBF /mv - 60. 2 ± 3. 6-30. 9 ± 8. 3 1 5 10 3 38. 1 ± 3. 5 2 5 mm 1 4% SABC Image-Pro Plus 5. 0 10. 0 20. 0 39. 4 ± 5. 4 2 48. 5 ± 4. 4 3 58. 1 ± 3. 1 3 5. 1 P < 0. 01 P < 0. 05 P < TFF1 0. 01 2 ~ 3 5 3. 2 IA 5 IA 1 A. B. C. 5 mg kg - 1 D. 5 g kg - 1 E. 10 g kg - 1 F. 20 g kg - 1 2 1 Fig. 1 HE 200 Histopathological effect of Atractylodex Rhizoma extract on gastric mucosa in model rats HE 200 3. 3 102
21 13 2 2 8 000 Fig. 2 Influence of Atractylodex Rhizoma extract on cells ultrastructural of gastric mucosa in model rats transmission electron microscopy 8 000 3. 4 TFF1 P < 0. 01 P < 0. 01 TFF1 P < 0. 05 P < 0. 01 P < 0. 05 P < 0. 01 - - 2 2 x 珋 ± s n = P < 0. 01 10 Table 2 Influence of Atractylodex Rhizoma extract on gastric emptying and small intestinal propulsion of model rat x 珋 ± s n = 10 /g kg - 1-26. 2 ± 3. 6 74. 4 ± 6. 8-69. 9 ± 8. 3 1 29. 8 ± 4. 4 1 5 10 3 29. 1 ± 3. 5 2 62. 4 ± 5. 7 2 5. 0 52. 4 ± 5. 4 2 40. 2 ± 7. 2 2 /% /% 10. 0 39. 5 ± 4. 4 3 53. 8 ± 6. 1 3 20. 0 28. 1 ± 3. 1 3 72. 7 ± 5. 1 3 P < 0. 05 P < 0. 01-3 4 4 5 3. 5 TFF1 TFF1 6 103
21 13 3 TFF1 c- kit x 珋 ± s n = 10 Table 3 Influence of Atractylodes Rhizoma extract on gastric mucosa tissues TFF1 colon tissue expression of model rat x 珋 ± s n = 10 /g kg - 1 TFF1 /IA /IA - 11. 01 ± 2. 38 16. 27 ± 4. 01-4. 06 ± 1. 18 1 5. 69 ± 1. 21 1 5. 0 10-3 8. 13 ± 1. 66 2 9. 01 ± 2. 32 2 5. 0 6. 36 ± 1. 01 2 8. 09 ± 1. 89 2 10. 0 8. 04 ± 1. 53 3 12. 07 ± 2. 87 3 20. 0 9. 98 ± 2. 06 3 15. 98 ± 3. 81 3 1. J. 2012 18 20 318-320. 2. J. 2009 34 20 2669-2671. 3. ATPase J. 2011 29 2 24-27. 4. - - J. 2014 4 119-122. - 5. J. GMBF 2011 19 29 3028-3030. 7 TFF1 6. J. 2013 29 2 8 202-205. 7. J. 2013 11 24 59-61. 1 8. TFFI EGF EGFR J. 2013 33 ICC 10 1649-1651. 9 ICC 9. Cajal ICC Ca 2 + J. 2013 29 11 10 1872-1875. 10. Rb1 Cajal J. 2013 24 10 2541-2543. ICC 104