256 ( ) ratsinothergroupswerefedwithxiaochengqidecoctionplushungertoestablishthespleendeficiencysyndrome models.afterthemodelestablishingt

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1 ( ) JournalofJilinUniversity(MedicineEdition) Vol.41 No.2 Mar [ ] Ⅹ(2015) DOI: /j x ( ; ) [ ] : :60 SD g ml -1 ; IgA ; IgG (MTL) P (SP) (SS) ; Tol 4 (TLR4) : IgA IgG MTL SP SS (P<0.05P<0.01); IgA IgG MTL SP SS TLR4 (P<0.05 P<0.01) : [ ] ; ; ; [ ] R285.5 [ ] A EffectofRhizoma Atractylodisextractongastrointestinalmotility andimmunefunctionofratswithexperimentalspleen deficiencysyndrome LIU Fen 12 LIU Yanju 1 TIAN Chunman 2 (1.DepartmentofChineseMedicineChemistryColegeofPharmacyHubeiTraditionalChineseMedicine UniversityWuhan430065China;2.DepartmentofChineseMedicineColegeofTraditionalChinese MedicineHubeiMinzuUniversityEnshi445000China) Abstract:Objective ToobservetheefectofRhizomaatractylodisextractonthegastrointestinalmotilityand immunefunctionoftherats withexperimentalspleendeficiencysyndromeandtoelucidatethe mechanism of Rhizomeatractylodisextractonregulatingthegastrointestinaldynamicandimmunefunction.Methods 60 male SDrats wererandomly dividedinto normalgroup modelgrouplow medium and high doses ofrhizome atractylodisextractgroupsdomperidonegroup;therewere10ratsineachgroup.exceptfornormalgroupthe [ ] [ ] ( ); ( ); (2011ZDXK-02) [ ] (1977-) [ ] (Tel: lyj @sohu.com)

2 256 ( ) ratsinothergroupswerefedwithxiaochengqidecoctionplushungertoestablishthespleendeficiencysyndrome models.afterthemodelestablishingtheratsinlowmedium andhighdosesofrhizomeatractylodisextract groupsweregavaged with g ml -1 Rhizomeatraetylodis extractrespectively;theratsin domperidonegroupweregavagedwiththedomperidonesuspensionsolutionandtheratsinnormalgroupandmodel groupweregavagedwithphysiologicalsaline.charcoalgavagemethodwasusedtodeterminetheratioofstomach retentionandtherateofsmalintestinalpropulsionoftherats;intestinalperfusionmethodwasusedtodetectthe levelsofigainintestinalperfusionliquidoftherats;themethodofdrawingbloodfromabdominalaortawasusedto detectthelevelsofigginserumandmotilin (MTL)substanceP (SP)somatostatin (SS)inplasmaoftherats; immunohistochemistry method was used to detectthe expressionlevels of Tol-like receptor 4 (TLR4)in duodenumjejunumandileumtissues.results Comparedwith modelgroupthelevelsofigaintheintestinal perfusionfluidandiggintheserum oftheratsinlowmediumandhighdosesofrhizomeatractylodisextract groupsanddomperidonegroupwereincreased;theratesofstomachretentionoftheratsweredecreasedobviously andtheratiosofsmalintestinalpropulsionoftheratswereincreasedsignificantlywhilethelevelsofmtlsp andssintheplasmaoftheratswereincreased (P< 0.05orP<0.01).Compared withlow doseofrhizome atractylodisextractgroupthelevelsofigaintheintestinalperfusionliquidandiggintheserumoftheratsin mediumandhighdosesofrhizomeatractylodisextractgroupswereincreased;theratesofstomachretentionofthe ratsweredecreasedandtheratiosofsmalintestinalpropulsionoftheratswereincreasedwhilethelevelsofmtl SPandSSintheplasmaoftheratswereincreasedandthelevelsofTLR4intheduodenumjejunumandileum tissueswereincreased (P < 0.05P < 0.01).Conclusion Rhizomeatractylodis extractcanimprovethe gastrointestinalmotilityandtheimmunefunctionoftheratswithexperimentalspleendeficiencysyndromeandhas beterregulationandtreatmentefectsonthefunctionalgastrointestinaldisordersandlowstateofsystemicandlocal immunefunction. Keywords: Rhizome atractylodis extract;spleen deficiency syndrome; gastrointestinal function; mucosal immunity : [1] 1 : ( ) 5%~ 1.1 SD 10% 60 (200g±20)g :SKSF ( ) [2] : : 500g ( ) [3] : 80% 12h 80% 3 2h (500g L -1 ) ( [4] : ); ( )); [I 125 ] ( (motilinmtl) P (substancepsp) ) (somatostatinss) (

3 ); IgG IgA ( ); Tol 4 (Tol-likereceptor4 TLR4) ( ) DAB ( ) A GC-1500 γ ( IgG );DG5032 ( ELISA IgA ); 1.5 UT6062 ( 5% 2% CMC-Na );XSP-300 ( 2.5mL 30 min 1% );Image-ProPlus5.1 Motic ) ( 1.2 [5] : ( = / )1 60g kg %; 6g ml -1 10mL kg -1 1 = ( - )/ % ( g kg -1 ) (5.0 mg kg -1 ) 10 SS γ- γ- 15d 2 ml 2mL 1 10d 1.4 ELISA IgA ) TLR4 IgG 5mm ( ) 24h 4 1 4% 2mL 15min [5] mg ( ) 2 ml EDTA 5 Image- ProPlus g TLR4 10min 12% (IA) 5 (100mmol L -1 ) 50μL 15min 7% 250μL-20 50μL 450nm (A) 15min A 1.2mL 6mL 1.6 MTL SP SS MTL SP 1.7 ( IA IA 1.8 SPSSl7.0 ELISA IgA MTL SP SS 10 x±s t ;

4 258 ( ) IgA IgG ( Ig A IgG ) TLR4 x±s IgA IgG IgA (P<0.05 P<0.01) 1 IgG (P<0.01); (P<0.05 P<0.01); IgA IgG (P<0.05 P<0.01); Ig A IgG 1 IgA IgG Tab.1 LevelsofIgAinintestinalperfusionfluidandIgGinserumofratsinvariousgroups Group IgA IgG [n=10x±s ρ B/(g L -1 )] Normal 1.05± ±0.86 Model 0.29±0.02 * 2.02±0.15 * Domperidone 0.40± ±0.28 Rhizomaatractylodisextract Lowdose 0.44± ±0.26 Mediumdose 0.65±0.05 # 4.15±0.29 # Highdose 0.99±0.08 # # 5.67±0.78 # # * P<0.01vsnormalgroup; P<0.05 P<0.01vsmodelgroup; # P<0.05 # # P<0.01vsdomperidonegroup; P<0.05 P< 0.0lvslowdoseofRhizomaatractylodisextractgroup. 2.2 (P< ) (P<0.01); Tab.2 Rates ofstomach retention and ratios ofsmal intestinalpropulsionofratsinvariousgroups (n=10x±s η /%) (P<0.05 P<0.01); Rateofstomach Group retention (P>0.05) (P<0.05); Rhizomaatractylodisextract (P <0.05 P < 0.01) MTL SP SS * P<0.01vsnormalgroup; P<0.05 P<0.01vsmodel MTL SP SS (P<0.01); group; # P<0.05vsdomperidonegroup; P<0.05 P<0.0l vslowdoseofrhizomaatractylodisextractgroup. MTL SP SS (P<0.05 P<0.01); 2.4 ( ) TLR4 TLR4 MTL SP SS (P<0.05 P< 0.01); MTL SP SS TLR4 : 13.5±1.3 (P<0.05 P<0.01) 3 Ratioofsmal intestinalpropulsion Normal 26.2± ±6.8 Model 69.9±8.3 * 29.8±4.4 * Domperidone 29.1± ±5.7 Lowdose 52.4± ±7.2 Mediumdose 39.5± ±6.1 Highdose 28.1± ±5.1 # 9.8± ± ±0.4

5 ± ±0.6 TLR4 6.9± ± ±1.0 (P<0.05 P<0.01); 9.3± ± ± ± ± TLR ± ±0.6 (P<0.05 P<0.01); 5.3± ±1.0 TLR4 TLR4 (P<0.01); (P<0.05 P<0.01) TLR4 1 ( ) 3 MTL SP SS Tab.3 LevelsofMTLSPandSSinplasmaofratsinvariousgroups Group MTL SP SS [n=10x±s ρ B/(ng L -1 )] Normal 90.19± ± ±9.12 Model 40.28±5.21 * 4.21±1.26 * 39.45±9.01 * Domperidone 71.15± ± ±6.18 Rhizomaatractylodisextract Lowdose 55.14± ± ±4.63 Mediumdose 70.25± ± ±5.01 Highdose 88.54±7.34 # 9.01±1.05 # 69.13±8.45 # * P<0.01vsnormalgroup; P<0.05 P<0.01vsmodelgroup; # P<0.05vsdomperidonegroup; P<0.05 P<0.0lvslow doseofrhizomaatractylodisextractgroup. 3 [11] SS [6] [12] : [7] [8] - [9] [13] : MTL SP [10] : :

6 260 ( ) (1): [3] [14] : [4] lgg IgA [5] [15] : 25. IgA IgG [6] ; IgG ATP IgA [7] IgG IgA [8] TLRs 63. [9] [16] : [10] TLR4 TLR4 : TLR4 29 (1): [13] : TLR (1): [14] [ ] [1]. [16]. NASH [J]. TLR4mRNA [J] (29): (1): [2]. [J].. [J] (20):. [J] (1): ATPase [J] (2):24-. [J] (3):. [J] (2): [J] (5):61-. [J] (2): [J] (11): [11]. MTL GAS VIP [J] (1): [12]. [J] [J].. [J] (6): [15]. [J] (12):

舊.A B NKTCL tissue C D: Reactive hyperplasia of lymph node tissue; A B : 0-catenin protein; C D : c-myc protein. 图 2 NKTCL 和反应性增生淋巴组织中 (3-catenin 和 emyc 蛋白的表达 (SPX200) Fig.

7 舊.A B NKTCL tissue C D: Reactive hyperplasia of lymph node tissue; A B : 0-catenin protein; C D : c-myc protein. 图 2 NKTCL 和反应性增生淋巴组织中 (3-catenin 和 emyc 蛋白的表达 (SPX200) Fig. 2 Expressions of (3-catenin and c-myc proteins in NKTCL and reactive hyperplasia of lymph node tissues (SP X200) 義(seen on page 232 in paragraph) A C E: 3-catenin protein;b»df:c-myc protein; A»B: YTS cells;c D:SNK-6 cells;e F:Normal NK cells. 图 1 YTS SNK-6 和正常 NK 细胞中 p-catenin 和 emyc 蛋白的表达 SP X400) 講r: Ifr.gFig. 1 Expressions of (3-catcnin and c-myc proteins in YTS SNK-6 and normal NK cclls (SP X400) (seen on page 232 in paragraph) ; 謹藝:K.1.變A Control group B DM group C DM-f-H2S group D H2S group. 图 1 各组大鼠心肌组织形态学 (VG X 100) Fig. 1 Morphology of myocardium tissue of rats in various groups (VG X100) (seen on page 237 in paragraph) A mir-18a NC group; B mir-18a mimic group C mir-18a NC + 4Gy group D;miR-18a mimic+4gy group. 图 2 GFPLC3 法检测电离辐射后 SW116 细胞自噬形态学 Fig. 2 Morphology of autophagy of SW116 cells after ionizing irradiation dctcctcd by GFPLC3 method (seen mm on page 242 in paragraph) A Control group;b: Model group. Bar=20 ptm. 图 2 Nissl 染色观察 2 组大鼠海马细胞形态学 Fig. 2 Morphology of hippocampus cells of rats detected by Nissl staining (seen on page 247 in paragraph) A F: and mg L SiO? groups. 图 1 各组细胞凋亡形态学 (AO/EB X400) Fig. 1 Morphology of apoptotic cells in various groups (AO/EB X 400) (seen 011 page 252 in paragraph) 轉 r 讀 A : Normal group B Model group;c:domperidone group;d F: Low»medium»and high doses of Rhizoma atractylodis extract groups. 图 1 各组大鼠十二指肠组织中 TLR4 的表达 ( 免疫组织化学 X200) Fig. 1 Expressions of TLR4 in duodenum tissue of rats in various groups (Immunohistochcmistry X 200) (seen on page 259 in paragraph)

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30 21 13 1 2 1* 2 1. 430065 2. 445000 20 10 5 g kg - 1 5 mg kg - 1 ig 10 d ig HE 1 TFF1 TFF1 P < 0. 01 P < 0. 01 P < 0. 01 P < 0. 05 R285. 5 doi 10. 13422 /j. cnki. syfjx. 2015130100 A 1005-9903 2015 13-0100-05