Agilent ZORBAX
Agilent ZORBAX HPLC... phzorbax 3 1. 6-9 2. www.agilent.com/chem/lccolumntips 3. 800-820-3278
...2 ZORBAX...5........................................................................ 6 ZORBAX.................................................................. 7 ZORBAX........................................................ 8................................................................ 10 USP................................................................ 12...................................................................... 13.............................................................................. 18 ZORBAX...19 ZORBAX Eclipse Plus......................................................................... 20 ZORBAX Eclipse XDB......................................................................... 26 ZORBAX 80Å StableBond..................................................................... 33 ZORBAX Rx................................................................................. 39 ZORBAX 80Å Extend-C18..................................................................... 41 ZORBAX Bonus-RP.......................................................................... 45 ZORBAX...................................................................... 49 ZORBAX...................................................................... 50 ZORBAX........................................................................ 51 ZORBAX........................................................................ 52...55 ZORBAX 1.8 µm............................................................. 57 ZORBAX 3.5 µm............................................................... 64 ZORBAX.......................................................................... 68 ZORBAX 1.0 mm.............................................................. 71 ZORBAX.................................................................... 73 ZORBAX PrepHT........................................................................... 80............................................................................ 84 Ultron................................................................................ 87 ZORBAX...89 ZORBAX 300Å StableBond.................................................................... 94 ZORBAX 300Å Extend-C18.................................................................... 99 ZORBAX Poroshell................................................................. 102 ZORBAX Eclipse (AAA).......................................................... 105 ZORBAX GF-250 GF-450...................................................... 107 ZORBAX SAX SCX............................................................ 111
ZORBAX Eclipse Plus LC Eclipse Plus 600 bar ZORBAX (RRHT) 1.8 µm LC ZORBAX Eclipse LC 40 2 www.agilent.com/chem/store
Agilent 1200 20 1200 HPLC 40 Agilent 1200 ZORBAX RRHT 1.8 µm www.agilent.com/chem/1200rr LC 60% 600 30 60,000 www.agilent.com/chem/cn 1200 ZORBAX RRHT 1200 HPLC www.agilent.com/chem/store 3
ZORBAX Eclipse ZORBAX Eclipse Eclipse Eclipse Eclipse 1.8 µm 7 µm... ph 1.8 µm 7 µm 600 bar ZORBAX 20 60% 4 www.agilent.com/chem/store
ZORBAX USP Agilemt ZORBAX HT Eclipse Plus USP ph ph www.agilent.com/chem/store 5
2007-2008 LC 52-53 52-53 H 2 O H 2 O 19 >2000 19 19 19 19 >2000 107 111 94 "Practical HPLC Methodology and Applications," Brian A. Bidlingmeyer, John Wiley & Sons, Inc., New York, p. 109 6 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX RP-HPLC Eclipse Plus ph 2-9 1.8 3.5 5 µm QA/QC 20-21 Eclipse XDB StableBond (SB) ph (ph 2-9) 1.8 3.5 µm ph C18 90 C C8 C3 Aq 80 C ph C18 C8 C3 CNAq (TFA) LC/MS (TFA) 1.8 3.5 µm 26 33 ZORBAX Rx Bonus-RP Extend-C18 ph SB Rx-C8 SB-C8 ph ph ph 3.5 µm pka ph ph 11.5 LC/MS ph 3.5 µm 39 45 41 ZORBAX ZORBAX ph SB SB ph 51 ZORBAX ODS Classic ph SB XDB 51 www.agilent.com/chem/store 7
ZORBAX HPLC HPLC ZORBAX MW < 2000 MW > 2000 80-20Å 300Å 80-20Å 300Å (300SB) Eclipse Plus C18 StableBond 300SB-C18 C18 C18 4.6 mm 4.6 mm LC/MS (mm) 4.6 0.1-1.5 mg 0.5-3 ml/min 3.0 150-500ug 0.3-1.5 ml/min + HPLC 2.1 50-120 ug 0.1-0.5 ml/min ++ LC/MS 1.0 10-50 ug 10-100 µl/min ++++ LC/MS 0.5, 0.3 1-10 ug 1-15 µl/min +++++ LC/MS 0.1, 0.075 100-200 ng 200-500 nl/min ++++++ LC/MS 9.4 1-10 mg 5-10 ml/min mg 21.2 20-250 mg 20-60 ml/min 1 8
ZORBAX MW < 2000 MW > 2000 5µm 5µm 5 µm 3.5µm HT 1.8µm 3.5µm 5 µm 60% 1.8 µm 200% 3.5µm Poroshell 5µm 3.5 µm Poroshell 150 mm 150 mm 5 µm 150 mm 250 mm 20-30 (3.5 µm) 75 mm 50 mm 150mm 30-60 (3.5 µm) (50 mm) Eclipse Plus C18 4.6 x 150 mm, 5 µm 959993-902 Eclipse Plus C18 4.6 x 75 mm, 3.5 µm 959933-902 ZORBAX 300SB-C18 4.6 x 150 mm, 5 µm 883995-902 ZORBAX 300SB-C18 4.6 x 50 mm, 3.5 µm 865973-902 600 bar Eclipse Plus C18 4.6 x 50 mm, 1.8 µm 959941-902 ZORBAX Poroshell 300SB-C18 2.1 x 75 mm, 5 µm 660750-902 9
HPLC 5000 (60-100Å) 300Å HPLC 5 µm 3.5 µm 1.8 µm 3.5 µm 1.8 µm 3.5 µm LC 50 mm 1.8 µm RRHT LC LC 400 bar 4.6 x 150 mm 4.6 x 75 mm (4.6 x 250 mm) 2.1 3.0 mm ZORBAX ZORBAX SIL ZOR- BAX Rx-SIL ZORBAX SIL ZORBAX Rx-SIL ZORBAX Rx-SIL Eclipse StableBond ZORBAX SIL 10 www.agilent.com/chem/store
ZORBAX C18 C8 C3 CN C8 C18 C8 ph (THF) 200 nm ph ph ph ph ph 2 4 ph HPLC / www.agilent.com/chem/store 11
USP (USP) USP USP L1 L1 L7 L11 (RRHT) USP USP (µm) (Å) L1 1.5 10 µm 1.8, 3.5, 5, 7 ZORBAX Eclipse Plus C18 ZORBAX Eclipse Plus XDB-C18 ZORBAX StableBond SB-C18 ZORBAX Extend-C18 ZORBAX Rx-C18 ZORBAX ODS ZORBAX ODS Classic L3 5 10 µm ZORBAX Sil ZORBAX Rx-Sil L7 L8 L10 L11 L13 L14 L20 L33 L35 L56 L57 1.5 10 µm 10 µm 3 10 µm 1.5 10 µm 3 10 µm 10 µm 3 10 µm 4,000 400,000 ph 3 10 µm 5 µm 120 ZORBAX Eclipse Plus C8 ZORBAX Eclipse XDB-C8 ZORBAX SB-C8 ZORBAX Rx-C8 ZORBAX C8 3, 5 5 5 5 1.8, 3.5, 5, 7 3.5, 5 3, 5 95 80 80 80 80 70 70 70 80 95 80 80 80 70 ZORBAX NH2 5 70 ZORBAX Eclipse XDB-CN ZORBAX SB-CN ZORBAX CN ZORBAX Eclipse XDB-Phenyl ZORBAX SB-Phenyl ZORBAX 3.5, 5 3.5, 5 3, 5 3.5, 5 1.8, 3.5 5 80 80 70 80 80 70 ZORBAX TMS 5 70 ZORBAX SAX 5 70 ZORBAX GF-250 4 150 ZORBAX GF-250 4 150 ZORBAX GF-250 4 6 150 ZORBAX SB-C3 3.5, 5 80 Ultron ES-OVM 5 120 12 www.agilent.com/chem/store
ZORBAX ph 1-12 ph ph ph ph ph ph 1 ph ph ph ph ph 1 HPLC ph pka ph pka 6.5 ph < 3 - A ph 7 - B ph > 9 - C ph RP-HPLC / ph ph LC/MS ph (TFA) pka ph ph SiOH ph 4-5 SiO TEA Rx-SIL Rx-SIL ph www.agilent.com/chem/store 13
ph (ph 2-3) ph ph 2-3 ph ph ph ph 20-50 mm (ph 2-3) LC/MS ph LC/MS ph ZORBAX Eclipse Plus ZORBAX Eclipse Plus C18 C8 ph Eclipse Plus Eclipse Eclipse Plus ph 2-9 ph 2 14 www.agilent.com/chem/store
ZORBAX ph ph Eclipse Plus Eclipse XDB C18 C8 StableBond SB-C18 SB-C8 SB-Phenyl SB-CN SB-C3 SB-Aq ph ph ph 1-2 StableBond ph www.agilent.com/chem/store 15
ph (4-9) ZORBAX Eclipse Plus ph ph Eclipse Plus C18 ph Eclipse Plus ph 9 ph - ph ph 6-9 ph ph ph Eclipse Plus C18 ph=7 ph 6.1-8.1 ph 3.8-5.8 LC/MS ZORBAX Eclipse XDB-Phenyl CN Bonus-RP ph ph Eclipse XDB-Phenyl Eclipse XDB-CN Bonus-RP Bonus-RP 100% 16 www.agilent.com/chem/store
ZORBAX ph 3 2a Eclipse XDB 60 C Stablebond 80-90 C ph 7 ph 9 1 ZORBAX Eclipse Plus C18 C8 ph 2.5 (2-3) 20-50 mm T=30 C 60 C 0.5 < k < 20 2 5% (v/v) 3 0.5 < k < 20 2 4 Eclipse XDB-C18 C8 SB-CN AQ Bonus-RP 1 5 ZORBAX Eclipse Plus C18 C8 ph 7 (6-9) 20-50 mm T = 30 C 40 C 0.5 < k < 20 6 5% (v/v) 7 0.5 < k < 20 6 8 ZORBAX Eclipse XDB Bonus-RP 5 9 ZORBAX Extend-C18 ph 10.5 (9-11.5) 5-20 mm 10-50 mm T = 25 C 40 C 0.5 < k < 20 ph ph 1a ZORBAX SB-C18 SB-C8 ph 1-3 20-50 mm T = 30 C 90 C 0.5 < k < 20 1c 5% (v/v) 5c 5% (v/v) 1b 10 mm 25-50 mm ph 3 0.5 < k < 20 1d 5% (v/v) 1e 0.5 < k < 20 5a 10 mm 25-50 mm ph 7 0.5 < k < 20 5b 5d 0.5 < k < 20 10a 10 0.5 < k < 20 HPLC 17
HPLC Agilent HPLC 2 3 4 4.6 mm ZORBAX 400 bar PEEK 1/16 LC ZORBAX 3.5 µm 1.8 µm ZORBAX ZORBAX Prep LC/MS LC/MS/MS Eclipse XDB ph 2-9 StableBond ph 2000 psi 135 bar 13.5 MPa 1/16 LC 21.2 30 mm 18 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX ph ZORBAX ZORBAX Eclipse Plus HPLC ZORBAX Eclipse XDB HPLC ZORBAX StableBond HPLC ph ZORBAX Rx HPLC ph 9 ZORBAX Extend-C18 HPLC ph ZORBAX Bonus-RP HPLC - ZORBAX ZORBAX ZORBAX HPLC A www.agilent.com/chem/store 19
ZORBAX Eclipse Plus LC... Eclipse Plus Eclipse Plus Eclipse Eclipse Plus Eclipse Plus 1.8 3.5 5 µm LC LC LC Eclipse 1.8 µm 7 µm 20 www.agilent.com/chem/store
ZORBAX ph HPLC 1.8µm Eclipse Plus ph ZORBAX Eclipse Plus C18 95Å 160 m 2 /g 60 C 2.0-9.0 8% ZORBAX Eclipse Plus C8 95Å 160 m 2 /g 60 C 2.0-8.0 8% www.agilent.com/chem/store 21
Eclipse Plus: : Eclipse Plus C18 Ace 5 C18 SunFireC18 XBridgeC18 Eclipse Plus C18 959996-902 4.6 x 100 mm, 5 µm 1 2 : : : : A 60% B 40% 1.0 ml/min UV 254 nm 5989-4934EN DiscoveryHS C18 LunaC18 (2) GeminiC18 XTerra MS C18 1. 2. 0 0.5 1 1.5 2 2.5 3 3.5 min Eclipse Plus C18 C8 RRHT RR A B Eclipse Plus C18 959941-902 4.6 x 50 mm, 1.8 µm Eclipse Plus C8 959943-906 4.6 x 50 mm, 3.5 µm 1. Oxybenzone 2. Internal Std. 3. Octylmethoxycinnamate : : (30:70) : 2.0 ml/min : 30 C : UV 230 nm 100 C 0.45 µm 1. 2. 3. 247 bar N: 8800 Pw: 0.014 min. TF: 1.20 N: 11600 Pw: 0.047 min. TF: 1.06 N: 11800 Pw: 0.093 min TF: 1.02 0 1 2 3 4 min N: 5000 Pw: 0.019 min. TF: 1.09 N: 6600 Pw: 0.037 min. TF: 0.99 N: 6300 Pw: 0.061 min. TF: 0.98 22 min. 88 bar 0 1 2 3 4 min C8 RRHT C8 22 www.agilent.com/chem/store
ZORBAX ph 2 ph 7 : 1. 2. 3. 4. Eclipse Plus C8 959946-906 4.6 x 50 mm, 5 µm 1 : 3 10-60% B/3 min ph 2.7 A 0.1% B 0.1% fa ph 7.0 A 20 mm B: Excedrin ph 2.7 2 4 0 0.5 1 1.5 2 2.5 3 3.5 m 1 ph 7.0 3 4 2 2 min. 0 0.5 1 1.5 2 2.5 3 3.5 m Eclipse Plus C18 C8 ph Eclipse Plus C8 Eclipse Plus C18 A B Eclipse Plus C8 959996-906 4.6 x 100 mm, 5 µm Eclipse Plus C18 959996-902 4.6 x 100 mm, 5 µm : : : 80% 8mM K 2 HPO 4 ph 7 1.0 ml/min UV 215 nm 0.05 µg/µl 0.5 µl 3.789 Tf=1.04 0 1 2 3 4 5 6 7 8 9 min 5.777 Tf=1.00 0 1 2 3 4 5 6 7 8 9 min C8 C18 Eclipse Plus C8 C18 www.agilent.com/chem/store 23
ZORBAX Eclipse Plus A XBridge C18 4.6 x 150mm 5µm A 1 N = 14000 Tf = 1.25 2 3 4 5 N = 41000 Tf = 1.13 6 B Eclipse Plus C18 959993-902 4.6 x 150 mm, 5 µm 0 2 4 6 8 10 12 min B 1 N = 23000 Tf = 1.01 N = 60000 Tf = 1.00 2 3 4 5 6 0 2 4 6 8 10 12 min : A 0.1% B 0.1% : 1.0 ml/min : 0.0 min 10% B 15 min 30% B : 40 C : UV 254 nm 5989-4934EN 1. 2. 3. 4. 5. 6. Eclipse Plus A Eclipse Plus C18 959941-902 4.6 x 50 mm, 1.8 µm A Tf = 1.00 O O N + Cl - OCH 3 1. : A 50% 8 mm K2HPO4 ph 7 B 50% ACN : 1.0 ml/min : : UV 254 nm 5989-4934EN 0.4 mg/ml 2 ul 0 0.5 OCH 3 1 1.5 2 2.5 min B Eclipse Plus C18 959993-902 4.6 x 150 mm, 5 µm 959993-902 B Tf = 1.06 0 0.5 1 1.5 2 2.5 min 24 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse Plus Eclipse Plus C18 USP L1 Eclipse Plus C8 USP L7 (mm) (µm) 4.6 x 250 5 959990-902 959990-906 4.6 x 150 5 959993-902 959993-906 4.6 x 100 5 959996-902 959996-906 4.6 x 50 5 959946-902 959946-906 4.6 x 150 3.5 959963-902 959963-906 4.6 x 100 3.5 959961-902 959961-906 4.6 x 75 3.5 959933-902 959933-906 4.6 x 50 3.5 959943-902 959943-906 4.6 x 30 3.5 959936-902 959936-906 600 bar 4.6 x 150 1.8 959994-902 600 bar 4.6 x 100 1.8 959964-902 959964-906 600 bar 4.6 x 50 1.8 959941-902 959941-906 600 bar 4.6 x 30 1.8 959931-902 959931-906 3.0 x 150 5 959993-302 959993-306 3.0 x 150 3.5 959963-302 959963-306 3.0 x 100 3.5 959961-302 959961-306 600 bar 3.0 x 100 1.8 959964-302 959964-306 600 bar 3.0 x 50 1.8 959941-302 959941-306 2.1 x 150 5 959701-902 959701-906 2.1 x 50 5 959746-902 959746-906 2.1 x 150 3.5 959763-902 959763-906 2.1 x 100 3.5 959793-902 959793-906 2.1 x 50 3.5 959743-902 959743-906 2.1 x 30 3.5 959733-902 959733-906 600 bar 2.1 x 100 1.8 959764-902 959764-906 600 bar 2.1 x 50 1.8 959741-902 959741-906 600 bar 2.1 x 30 1.8 959731-902 959731-906 4/ 4.6 x 12.5 5 820950-936 820950-937 4/ 2.1 x 12.5 5 821125-936 821125-937 820888-901 820888-901 www.agilent.com/chem/store 25
O CH 3 Si ZORBAX Eclipse XDB O O CH 3 CH 3 Si CH 3 CH 3 Si CH 3 ph (ph 2-9) 1.8 µm 7 µm CH 3 CH 3 O Si CH 3 CH 3 CH 3 O Si CH 3 Eclipse XDB Agilent ZORBAX Eclipse XDB C18 C8 ph (ph 2-9) Eclipse XDB ph (2-3) ph (6-8) ph Eclipse XDB ph 2-9 1.8 3.5 5 7 µm Eclipse XDB C18 ph ZORBAX Eclipse XDB-C18 80Å 180 m 2 /g 60 C 2.0-9.0 10% ZORBAX Eclipse XDB-C8 80Å 180 m 2 /g 60 C 2.0-9.0 7.6% ZORBAX Eclipse XDB- 80Å 180 m 2 /g 60 C 2.0-9.0 7.2% Phenyl ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 60 C 2.0-8.0 4.3% 26 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse XDB ph : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 1.5 ml/min : 40 C A : ph 3.0 75% 25 mm ph 3.0 25% B : ph 7.0 90% 20 mm ph 7.0 10% ACN 1. 2. 3. 4. 5. 1. 2. N- 3. N- ZORBAX Eclipse XDB ph ph 2-9 ph 3 60 C : ZORBAX SB-C8 883975-906 4.6 x 150 mm, 5 µm : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 70% 50 mm NaAc-HCl ph 3.0 30% ACN 65% 35% : 1.0 ml/min : 60 C : ZORBAX SB-C8 P/N 883975-906 ZORBAX Eclipse XDB-C8 P/N 993967-906 Competitive C Eclipse XDB ph ph Eclipse ph 3 SB-C8 60 C ph 3 12,000 (amitriptyline) Eclipse XDB www.agilent.com/chem/store 27
ph 7.0 : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 60% ACN 40% 250 mm ph 7.0 : 1.5 ml/min : 60 C : 1. 2. 3. 4. 5. Rx-Sil sil-gel - ph 7 (60 C) (250 mm)- Eclipse XDB StableBond A Eclipse XDB-C8 966967-906 4.6 x 75 mm, 3.5 µm B ZORBAX Rx/SB-C8 866953-906 4.6 x 75 mm, 3.5 µm : 70% 25 mm NaH2PO4 ph 3.0 30% : 1.0 ml/min : 35 C 1. 2. 3. Time (min) Time (min) Eclipse XDB StableBond 28 www.agilent.com/chem/store
ZORBAX Eclipse XDB A B C Eclipse XDB-Phenyl 963967-912 4.6 x 150 mm, 3.5 µm Eclipse XDB-C8 963967-906 4.6 x 150 mm, 3.5 µm Eclipse XDB-C18 963967-902 4.6 x 150 mm, 3.5 µm : 15% :85% : 1.0 ml/min : 35 C : 1. 2. 3. -O Eclipse XDB C18 C8 Eclipse XDB-Phenyl Eclipse XDB-C8 : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 85% 25 mm Na 2 HPO 4 ph 7:15% : 1.0 ml/min : 35 C : 1. 2. 3. Cefoxatime 4. 5. Eclipse XDB-C8 A Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 µm 1. 2. 3. 4. 5. 6. B C Eclipse XDB-CN 993967-905 4.6 x 150 mm, 5 µm Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 µm : A. 60:40 : B. 60:40 : C. 77:23 : : 1.0 ml/min : 25 C : Eclipse XDB-C18 Eclipse XDB-CN www.agilent.com/chem/store 29
ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 XDB-Phenyl USP L11 XDB-CN USP L10 (, 400 bar) 9.4 x 250 5 990967-202 990967-206 4.6 x 250 5 990967-902 990967-906 990967-912 990967-905 4.6 x 150 5 993967-902 993967-906 993967-912 993967-905 4.6 x 50 5 946975-902 946975-906 4.6 x 150 3.5 963967-902 963967-906 963967-912 963967-905 4.6 x 100 3.5 961967-902 961967-906 961967-905 4.6 x 75 3.5 966967-902 966967-906 966967-912 966967-905 4.6 x 50 3.5 935967-902 935967-906 935967-912 600 bar 4.6 x 100 1.8 928975-902 928975-906 600 bar 4.6 x 50 1.8 927975-902 927975-906 600 bar 4.6 x 30 1.8 924975-902 924975-906 600 bar 4.6 x 20 1.8 926975-902 926975-906 3.0 x 250 5 990967-302 990967-306 990967-312 990967-305 3.0 x 150 5 993967-302 993967-306 993967-312 993967-305 3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 600 bar 3.0 x 100 1.8 928975-302 928975-306 600 bar 3.0 x 50 1.8 927975-302 927975-306 600 bar 3.0 x 30 1.8 924975-302 924975-306 600 bar 3.0 x 20 1.8 926975-302 926975-306 2.1 x 150 5 993700-902 993700-906 993700-912 993700-905 2.1 x 50 5 960967-902 960967-906 960967-912 960967-905 * 2.1 x 150 3.5 930990-902 930990-906 * 2.1 x 100 3.5 961753-902 961753-906 961753-905 * 2.1 x 75 3.5 966735-902 * 2.1 x 50 3.5 971700-902 971700-906 600 bar** 2.1 x 100 1.8 928700-902 928700-906 600 bar** 2.1 x 50 1.8 927700-902 927700-906 600bar** 2.1 x 30 1.8 924700-902 924700-906 600 bar** 2.1 x 20 1.8 926700-902 926700-906 * 1.0 x 150 3.5 963600-902 963600-906 * 1.0 x 50 3.5 965600-902 965600-906 * 1.0 x 30 3.5 961600-902 961600-906 3/ 1.0 x 17 5 5185-5921 5185-5921 4/ 4.6 x 12.5 5 820950-925 820950-926 820950-927 820950-935 4/ 2.1 x 12.5 5 821125-926 821125-926 821125-926 821125-935 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm **RRHT 1.8 µm 30 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 977250-102 977250-106 PrepHT 21.2 x 150 7 977150-102 977150-106 PrepHT 21.2 x 150 5 970150-902 970150-906 PrepHT 21.2 x 100 5 970100-902 970100-906 PrepHT 21.2 x 50 5 970050-902 970050-906 PrepHT 17 x 7.5 5 820212-925 820212-926 820444-901 820444-901 PrepHT 2/ 820400-901 820400-901 XDB-Phenyl USP L11 XDB-CN USP L10 (mm) ( 5021-1845) 4.6 x 250 5 7995118-585 7995108-585 4.6 x 150 5 7995118-595 7995108-595 4.6 x 75 3.5 7995118-344 7995108-344 3.0 x 75 3.5 7995230-344 10/ 4.0 x 4 5 7995118-504 7995118-504 5021-1845 5021-1845 (, 400 bar) 4.6 x 50 1.8 922975-902 922975-906 3/ 4.6 x 50 1.8 922975-932 (µm) XDB-C18 USP L1 2.1 x 50 1.8 922700-902 3/ 2.1 x 50 1.8 922700-932 XDB-C8 USP L7 31
ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 ( 820555-901) 4.6 x 30 3.5 933975-902 933975-906 3/ 4.6 x 30 3.5 933975-932 933975-936 4.6 x 15 3.5 931975-902 931975-906 3/ 4.6 x 15 3.5 931975-932 931975-936 2.1 x 30 3.5 973700-902 973700-906 3/ 2.1 x 30 3.5 973700-932 973700-936 2.1 x 15 3.5 975700-902 975700-906 3/ 2.1 x 15 3.5 975700-932 975700-936 4.6 x 50 1.8 925975-902 3/ 4.6 x 50 1.8 925975-932 4.6 x 30 1.8 923975-902 3/ 4.6 x 30 1.8 923975-932 4.6 x 15 1.8 921975-902 3/ 4.6 x 15 1.8 921975-932 2.1 x 50 1.8 925700-902 3/ 2.1 x 50 1.8 925700-932 2.1 x 30 1.8 923700-902 3/ 2.1 x 30 1.8 923700-932 2.1 x 15 1.8 921700-902 3/ 2.1 x 15 1.8 921700-932 820555-901 820555-901 0.5 x 250 5 5064-8286 0.5 x 150 5 5064-8287 0.5 x 150 3.5 5064-8288 0.5 x 35 3.5 5064-8298 0.3 x 250 5 5064-8269 0.3 x 150 5 5064-8291 0.3 x 150 3.5 5064-8271 0.5 x 35 5 5064-8296 0.3 x 35 5 5064-8297 32 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å StableBond ph ph 1 ph SB-C18 SB-C8 SB-CN SB-Phenyl SB-C3 SB- Aq B Agilent ZORBAX StableBond (SB-C18) SB-C8 SB-C3 SB-Phenyl SB-CN SB-Aq ph StableBond StableBond ph ZORBAX StableBond O OH O OH R Si R R Si R R R 1 R 1 * ph * ZORBAX 80Å 180 m 2 /g 90 C 1.0-8.0 10% SB-C18 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 5.5% SB-C8 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 4% SB-C3 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 5.5% SB-Phenyl ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 4% SB-CN ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 SB-Aq *StableBond ph ph 6-8 <40 C 0.01-0.02 M ph Eclipse XDB Bonus-RP O Si R StableBond R 1 www.agilent.com/chem/store 33
StableBond SB-C18 ph ph 0.8 90 C : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm : ZORBAX Rx-C18 883967-902 4.6 x 150 mm, 5 µm : 50% /50% 1.0% : 90 C Relative Retention Stablebond SB-C18 -C18 ZORBAX Rx-C18 -C18 A-C18 B-C18 C-C18 D-C18 E-C18 Column Volumes of Mobile Phase Purge PH (0.8) (90 C) StableBond SB-C18 ZORBAX Rx-C18 StableBond SB-C18 ZORBAX SB-CN ph (ph 2.0 50 C) : ZORBAX SB-CN 883975-905 4.6 x 150 mm, 5 µm % k Remaining ZORBAX StableBond SB-CN StableBond ph CN Column Volumes SB-CN A ZORBAX SB-C18 866953-902 4.6 x 75 mm, 3.5 µm B ZORBAX SB-CN 866953-905 4.6 x 75 mm, 3.5 µm : 30% 70% 25mM NaH 2 PO 4 ph 2.5 : 1.0 ml/min : 35 C 1. 2. 3. 4. 5. Time (min) Time (min) SB-CN 50% 34 www.agilent.com/chem/store
ZORBAX RRHT mau 450 400 350 300 250 200 150 100 50 0 0 1 2 1 1 2 1 2 3 2 4 3 5 4 3 3 1 2 3 4 5 : (40%): 60% ph=2.4 25mM NaH 2 PO 4 : 1.85 ml/min 5 3, 4 & 5 1. 2. 3. 4. 5. 4 5 5 4 A SB-C18 827975-902 4.6 x 50 mm, 1.8 µm B SB-C8 827975-906 4.6 x 50 mm, 1.8 µm C ZORBAX SB-Phenyl 827975-912 4.6 x 50 mm, 1.8 µm D ZORBAX SB-CN 827975-905 4.6 x 50 mm, 1.8 µm min DAD 254 nm LC Agilent 1200 RR LC 1 µl StableBond 5 SB-Aq A ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm D ZORBAX SB-Phenyl 883975-912 4.6 x 150 mm, 5 µm B ZORBAX SB-C8 883975-906 4.6 x 150 mm, 5 µm E ZORBAX SB-CN 883975-905 4.6 x 150 mm, 5 µm C ZORBAX SB-C3 883975-909 4.6 x 150 mm, 5 µm SB-C3 StableBond SB-C3 Rx-SIL : 18.8 min 0-100% B A 50 mm NaH 2 PO 4 ph 2.5 95% / 5% B 50 mm NaH 2 PO 4 ph 2.5 47% / 53% : 1.0 ml/min : 26 C : : 254 nm 1. 2. 3. d- 4. 5. www.agilent.com/chem/store 35
ZORBAX 80Å StableBond (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 SB-CN USP L10 SB-C3 USP L56 SB-Phenyl USP L11 SB-Aq (, 400 bar) 9.4 x 250 5 880975-202 880967-201 880975-205 880975-209 880975-212 9.4 x 150 5 883975-202 9.4 x 100 5 884975-202 9.4 x 50 5 846975-202 4.6 x 250 5 880975-902 880975-906 880975-905 880975-909 880975-912 880975-914 4.6 x 150 5 883975-902 883975-906 883975-905 883975-909 883975-912 883975-914 4.6 x 50 5 846975-902 846975-906 846975-914 4.6 x 250 3.5 884950-567 4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914 4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914 4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914 4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914 4.6 x 150 1.8 829975-902 829975-906 829975-905 829975-912 600 bar 4.6 x 100 1.8 828975-902 828975-906 828975-905 828975-912 828975-914 600 bar 4.6 x 50 1.8 827975-902 827975-906 827975-905 827975-912 827975-914 600 bar 4.6 x 30 1.8 824975-902 824975-906 824975-905 824975-912 824975-914 600 bar 4.6 x 20 1.8 826975-902 826975-906 600 bar 3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314 3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314 3.0 x 150 1.8 829975-302 829975-306 829975-305 829975-312 600 bar 3.0 x 100 1.8 828975-302 828975-306 828975-305 828975-312 828975-314 600 bar 3.0 x 50 1.8 827975-302 827975-306 827975-305 827975-312 827975-314 600 bar 3.0 x 30 1.8 824975-302 824975-306 824975-305 600 bar 3.0 x 20 1.8 826975-302 826975-306 600 bar 2.1 x 150 5 883700-922 883700-906 883700-905 883700-909 883700-912 2.1 x 50 5 860975-902 860975-906 860975-905 860975-909 860975-912 860975-914 * 2.1 x 150 3.5 830990-902 830990-906 830990-914 * 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914 * 2.1 x 75 3.5 866735-902 * 2.1 x 50 3.5 871700-902 871700-906 871700-914 *RR 3.5 µm **RRHT 1.8 µm 36 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å StableBond SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl (mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq 2.1 x 150 1.8 820700-902 820700-906 820700-905 820700-912 600 bar** 2.1 x 100 1.8 828700-902 828700-906 828700-905 828700-912 828700-914 600 bar** 2.1 x 50 1.8 827700-902 827700-906 827700-905 827700-912 827700-914 600 bar** 2.1 x 30 1.8 824700-902 824700-906 824700-905 824700-912 824700-914 600 bar** 2.1 x 20 1.8 826700-902 826700-906 600 bar** * 1.0 x 150 3.5 863600-902 863600-906 863600-905 * 1.0 x 50 3.5 865600-902 865600-906 * 1.0 x 30 3.5 861600-902 861600-906 1.0 x 17 5 5185-5920 5185-5920 3/ 2/ 9.4 x 15 7 820675-115 820675-115 820675-124 820675-115 4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-922 820950-917 820950-933 4/ 2.1 x 12.5 5 821125-915 821125-915 821125-924 821125-924 821125-915 821125-933 9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877250-102 877250-106 877250-105 877250-112 877250-114 PrepHT 21.2 x 150 7 877150-102 877150-106 877150-114 PrepHT 21.2 x 150 5 870150-902 870150-906 870150-914 PrepHT 21.2 x 100 5 870100-902 870100-906 870100-914 PrepHT 21.2 x 50 5 870050-902 870050-906 870050-914 PrepHT 17 x 7.5 5 820212-920 820212-915 820212-915 820212-915 820212-933 2/ 820444-901 820444-901 820444-901 820444-901 820444-901 820444-901 PrepHT 2/ *RR 3.5 µm **RRHT 1.8 µm 820400-901 820400-901 820400-901 820400-901 820400-901 820400-901 ( 5021-1845) 4.6 x 250 5 7995218-585 7995208-585 (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 4.6 x 150 5 7995218-595 7995208-595 4.6 x 75 3.5 7995218-344 7995208-344 10/ 4.0 x 4 5 7995118-504 7995118-504 5021-1845 5021-1845 www.agilent.com/chem/store 37
ZORBAX 80Å StableBond (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 (, 400 bar) 4.6 x 50 1.8 822975-902 822975-906 3/ 4.6 x 50 1.8 822975-932 2.1 x 50 1.8 822700-902 3/ 2.1 x 50 1.8 822700-932 ( 820555-901) 4.6 x 30 3.5 833975-902 833975-906 3/ 4.6 x 30 3.5 833975-932 833975-936 4.6 x 15 3.5 831975-902 831975-906 3/ 4.6 x 15 3.5 831975-932 831975-936 2.1 x 30 3.5 873700-902 873700-906 3/ 2.1 x 30 3.5 873700-932 873700-936 2.1 x 15 3.5 875700-902 875700-906 3/ 2.1 x 15 3.5 875700-932 875700-936 4.6 x 50 1.8 825975-902 3/ 4.6 x 50 1.8 825975-932 4.6 x 30 1.8 823975-902 3/ 4.6 x 30 1.8 823975-932 4.6 x 15 1.8 821975-902 3/ 4.6 x 15 1.8 821975-932 2.1 x 50 1.8 825700-902 3/ 2.1 x 50 1.8 825700-932 2.1 x 30 1.8 823700-902 3/ 2.1 x 30 1.8 823700-932 2.1 x 15 1.8 821700-902 3/ 2.1 x 15 1.8 821700-932 820555-901 820555-901 0.5 x 250 5 5064-8258 0.5 x 150 5 5064-8256 0.5 x 35 5 5064-8254 * 0.5 x 150 3.5 5064-8262 0.5 x 35 3.5 5064-8260 0.3 x 250 5 5064-8257 0.3 x 150 5 5064-8255 0.3 x 35 5 5064-8253 0.3 x 150 3.5 5064-8261 *RR 3.5 µm **RRHT 1.8 µm 38 www.agilent.com/chem/store
ZORBAX ZORBAX Rx Eclipse XDB-C18 StableBond SB-C18 Rx-C18 ph StableBond SB-C18 12% 10% ph Rx-C18 Rx-C18 ph 9 Rx-C8 SB-C8 ph ZORBAX Rx-C18 80Å 180 m 2 /g 60 C 2.0-9.0 12% ZORBAX Rx-C8 80Å 180 m 2 /g 60 C 1.0-8.0 5.5% Rx-C18 : : : ZORBAX Rx-C18 880967-302 3.0 x 250 mm, 5 µm 35% :65% 0.5 ml/min 1. 2. Rx-C18 (USP) 4.6 x 250 mm 3.0 mm Rx- C18 60% www.agilent.com/chem/store 39
ZORBAX Rx Rx-C18 Rx-C8 (mm) (µm) USP L1 USP L7** 9.4 x 250 5 880967-202 880967-201 4.6 x 250 5 880967-902 880967-901 4.6 x 150 5 883967-902 883967-901 4.6 x 150 3.5 863967-902 863953-906 4.6 x 100 3.5 861967-902 861953-906 4.6 x 75 3.5 866967-902 866953-906 3.0 x 250 5 880967-302 880975-306 3.0 x 150 5 883967-302 883975-306 3.0 x 150 3.5 863967-302 863954-306 3.0 x 100 3.5 861967-302 861954-306 2.1 x 150 5 883700-902 883700-906 * 2.1 x 100 3.5 861767-902 861753-906 2/ 9.4 x 15 7 820675-115 820675-115 4/ 4.6 x 12.5 5 820950-914 820950-913 4/ 2.1 x 12.5 5 821125-915 821125-915 9.4 x 15 840140-901 840140-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877967-102 877250-106 PrepHT 21.2 x 150 7 877150-106 PrepHT 21.2 x 150 5 870150-906 PrepHT 21.2 x 100 5 870100-906 PrepHT 21.2 x 50 5 870050-906 PrepHT 2/ 820212-914 820212-915 820444-901 820444-901 PrepHT 2/ 820400-901 820400-901 *RR 3.5 µm **Rx-C8 SB-C8 40 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å Extend-C18 ph ph 11.5 ph LC/MS O Si C18 ZORBAX Extend-C18 C18-C18 ph ph ph ph ph ph 10.5 LC/MS ph Extend-C18 ph 2-11.5 Extend-C18 ph * ph ZORBAX Extend-C18 80Å 180 m 2 /g 60 C 2.0-11.5 12.5% * ph 8 60 C ph 8-11.5 40 C O Si C18 Silica Support Extend C-18 C18-C18 www.agilent.com/chem/store 41
Extend-C18 ph : : : : : : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm ph 7 30% 20 mm Na2HPO4 70% ph 11 30% 20 mm 70% 1.0 ml/min 254 nm 1. 2. 3. 4. 5. 6. 7. ph ph Extend-C18 ph Time (min) Time (min) Extend-C18 ph : : : : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm 20% 80% 0.1 M ph 10.0 1.0 ml/min ph W ZORBAX Extend-C18 ph Amount of Dissolved Silica (mg) ZORBAX Extend-C18 W Volume Eluent (L) 42 www.agilent.com/chem/store
ZORBAX Extend-C18 ph : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm : 80% 25 mm NaH2PO4 ph 3.0 20% : 1.0 ml/min : 35 C : 254 nm : 1. 2. 3. 4. Extend-C18 ph Extend-C18 ph ph Time (min) ZORBAX 80Å Extend-C18 (mm) (, 400 bar) 4.6 x 250 5 770450-902 4.6 x 150 5 773450-902 4.6 x 50 5 746450-902 4.6 x 150 3.5 763953-902 4.6 x 100 3.5 764953-902 4.6 x 75 3.5 766953-902 4.6 x 50 3.5 735953-902 600 bar 4.6 x 100 1.8 728975-902 600 bar 4.6 x 50 1.8 727975-902 400 bar 4.6 x 50 1.8 722975-902 600 bar 4.6 x 30 1.8 724975-902 600 bar 4.6 x 20 1.8 726975-902 3.0 x 250 5 770450-302 3.0 x 150 5 773450-302 3.0 x 150 3.5 763954-302 3.0 x 100 3.5 764953-302 3.0 x 50 3.5 735954-302 600 bar 3.0 x 100 1.8 728975-302 600 bar 3.0 x 50 1.8 727975-302 600 bar 3.0 x 30 1.8 724975-302 600 bar 3.0 x 20 1.8 726975-302 (µm) www.agilent.com/chem/store 43
ZORBAX 80Å Extend-C18 (mm) (µm) 2.1 x 150 5 773700-902 2.1 x 50 5 760450-902 * 2.1 x 100 3.5 761753-902 * 2.1 x 50 3.5 735700-902 600 bar** 2.1 x 100 1.8 728700-902 600 bar** 2.1 x 50 1.8 727700-902 600 bar** 2.1 x 30 1.8 724700-902 600 bar** 2.1 x 20 1.8 726700-902 * 1.0 x 150 3.5 763600-902 * 1.0 x 50 3.5 765600-902 * 1.0 x 30 3.5 761600-902 3/ 1.0 x 17 5 5185-5923 4/ 4.6 x 12.5 5 820950-930 4/ 2.1 x 12.5 5 821125-930 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 150 5 770150-902 PrepHT 21.2 x 100 5 770100-902 PrepHT 21.2 x 50 5 770050-902 PrepHT 2/ 820400-901 PrepHT 2/ 17 x 7.5 5 820212-930 820444-901 *RR 3.5 µm **RRHT 1.8 µm 44 www.agilent.com/chem/store
ZORBAX ZORBAX Bonus-RP ph ph 100% O CH 3 R 1 Si R 1 PG R Agilent ZORBAX Bonus-RP ph Bonus-RP C18 C8 Si CH 3 CH 3 R 1 O Si R 1 PG R CH 3 * ph ZORBAX Bonus-RP 80Å 180 m 2 /g 60 C 2.0-9.0 9.5% * ph 8 60 C ph 8-9 40 C Si CH 3 CH 3 R 1 O Si R 1 PG R Bonus-RP ZORBAX Bonus-RP A ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm B Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 75% 25 mm ph 6 25% : 1.0 ml/min : : 254 nm : 3 µl 1. 2. 3. 4. Time (min) Bonus-RP www.agilent.com/chem/store 45
Bonus-RP : Alkye-C8 4.6 x 150 mm 5 µm : 75% 25 mm NH 4 Ac ph 5.5 25% : 1.5 ml/min : 40 C : 254 nm 1. 2. 3. C8 Bonus-RP ph Bonus-RP TF 1. 1.8 2. 1.7 3. 2.1 Time (min) Bonus-RP 2 : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm : 80% 25 mm NH 4 Ac ph 5.5 20% : 1.5 ml/min : 40 C : 254 nm 1. 2. 3. TF 1. 1.1 2. 1.1 3. 1.1 Time (min) 46 www.agilent.com/chem/store
ZORBAX ph ph ZORBAX Bonus-RP : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm : 60% 25 mm ph 7.0:40% ACN : 1.5 ml/min : 23 C Toluene Plate Height, cm / Bonus-RP Column Volume of Purge Bonus-RP ph 7 10,000 -C18/ Bonus-RP : : : : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm 50% 50% 0.1% 80% 20% 1.0 ml/min 60 C 23 C Toluene Plate Height, cm -C18/ Bonus-RP Column Volume of Purge ph www.agilent.com/chem/store 47
ZORBAX Bonus-RP (mm) (, 400 bar) 4.6 x 250 5 880668-901 4.6 x 150 5 883668-901 4.6 x 150 3.5 863668-901 4.6 x 100 3.5 864668-901 4.6 x 75 3.5 866668-901 3.0 x 250 5 880668-301 3.0 x 150 5 883668-301 3.0 x 150 3.5 863668-301 3.0 x 100 3.5 864668-301 2.1 x 150 5 883725-901 2.1 x 50 5 861971-901 * 2.1 x 150 3.5 863700-901 * 2.1 x 100 3.5 861768-901 * 2.1 x 50 3.5 861700-901 * 1.0 x 150 3.5 863608-901 * 1.0 x 50 3.5 865608-901 * 1.0 x 30 3.5 861608-901 3/ 1.0 x 17 5 5185-5922 4/ 4.6 x 12.5 5 820950-928 4/ 2.1 x 12.5 5 821125-928 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 878250-101 *RR 3.5 µm (µm) PrepHT 21.2 x 150 7 878150-101 PrepHT 21.2 x 150 5 868150-901 PrepHT 21.2 x 100 5 868100-901 PrepHT 21.2 x 50 5 868050-901 PrepHT 2/ 820400-901 PrepHT 2/ 17 x 7.5 5 820212-928 820444-901 48 www.agilent.com/chem/store
ZORBAX ZORBAX ph 2-9 Eclipse ph StableBond ph (1-11.5) ph ph ZORBAX StableBond 4.6 x 150 mm 5 µm SB-C18 SB-CN SB-Phenyl StableBond 4.6 x 75 mm 3.5 µm SB-C18 SB-CN Eclipse XDB 4.6 x 150 mm 5 µm XDB-C18 XDB-C8 XDB-Phenyl Eclipse XDB 4.6 x 75 mm 3.5 µm XDB-C18 XDB-C8 ph 4.6 x 150 mm 5 µm SB-C18 XDB-C18 Extend-C18 ph 4.6 x 75 mm 3.5 µm SB-C18 XDB-C18 Extend-C18 4.6 x 150 mm 5 µm SB-Aq Bonus RP SB-C18 4.6 x 75 mm 3.5 µm SB-Aq Bonus RP SB-C18 5183-4624 5183-4625 5183-4626 5183-4627 5185-5807 5185-5808 5185-5809 5185-5810 www.agilent.com/chem/store 49
ZORBAX ZORBAX HPLC www.agilent.com/chem/lccustom 1-2 1 899999-888 2 3 4 5 (302) 993-5354 custom_columns@agilent.com 1-2 3 50 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX A (mm) (µm) ODS (C18) USP L1 C8 USP L7 USP L11 USP L10 TMS USP L13 (, 400 bar) 9.4 x 250 5 880952-202 880952-206 4.6 x 250 5 880952-702 880952-706 880952-712 884950-507 880952-710 4.6 x 250 5 884950-543 4.6 x 150 5 883952-702 883952-706 883952-712 884950-526 883952-710 3.0 x 250 5 880952-302 3.0 x 150 5 883952-302 ( ) 2/ 9.4 x 15 7 820675-115 820675-115 820675-115 820675-124 4/ 4.6 x 12.5 5 820950-902 820950-906 820950-912 820950-905 820950-924 9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877952-102 877952-106 877952-105 PrepHT 2/ 820400-901 820400-901 820400-901 www.agilent.com/chem/store 51
Zorbax ZORBAX Rx-SIL (>99.995%) ZORBAX-SIL ZORBAX Rx-SIL HILIC ZORBAX Eclipse XDB-CN Rx-SIL ZORBAX Rx-SIL ZORBAX CN ZORBAX SIL ZORBAX SIL ZORBAX NH2 - ZORBAX SIL HPLC A D 52 www.agilent.com/chem/store
ZORBAX ph ZORBAX Rx-SIL 80Å 180 m 2 /g 0-8.0 ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 2.0-8.0 4.3% ZORBAX SIL 70Å 300 m 2 /g 0-8.0 ZORBAX CN 70Å 300 m 2 /g 2.0-7.0 7% ZORBAX NH 2 70Å 300 m 2 /g 2.0-7.0 4% ZORBAX CN ZORBAX CN : ZORBAX CN 880952-705 4.6 x 250 mm, 5 µm : A B 2- / (50/50) : 2 ml/min : 10 min. B 2-20% 20% : 50 C : 278 nm (n = 10) 0 5 10 15 20 Time (min) www.agilent.com/chem/store 53
Zorbax (mm) (µm) XDB-CN USP L10 Rx-SIL (, 400 bar) 9.4 x 250 5 880975-201 880952-201 880952-205 880952-208 4.6 x 250 5 880975-901 880952-701 880952-705 880952-708 840300-908 990967-905* 4.6 x 150 5 883975-901 883952-701 883952-705 883952-708 843300-908 993967-905* 2.1 x 150 5 883700-901 993700-905* 2.1 x 50 5 860700-708 ( ) 2/ 9.4 x 15 5 820675-119 820675-119 820675-111 820675-111 4/ 4/ 4.6 x 12.5 5 820950-919 820950-901 820950-905 820950-908 820950-908 820950-935 2.1 x 12.5 5 821125-919 821125-935 9.4 x 15 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877250-101 877952-101 PrepHT 21.2 x 250 7 877952-105 877952-108 PrepHT 2/ 820400-901 820400-901 820400-901 820400-901 PrepHT 2/ 17 x 7.5 5 820212-919 820444-901 * 54 www.agilent.com/chem/store
ZORBAX (3.5 µm) (1.8 µm) Eclipse XDB StableBond Eclipse Plus RR RRHT ZORBAX 4.6 mm 50% HPLC LC/MS ZORBAX HPLC 2.1 mm 5 ZORBAX ZORBAX Ultron www.agilent.com/chem/store 55
ZORBAX (RR) ZORBAX 600 bar RRHT Agilent 1200 95% 1.8 µm ZORBAX LC RRHT LC, (RR) (RRHT) ZORBAX 1.8 µm Agilent 1200...... 20 100 HPLC 30-40% 56 www.agilent.com/chem/store
ZORBAX 1.8 µm 1.8 µm [600 bar] 2 25% 95% HPLC 80 50 mm ZORBAX (1.8 µm) 1.8 µm 250 mm 95% 80 RRHT ZORBAX Eclipse Plus ZORBAX (Eclipse XDB StableBond Extend) 3.5 µm 1.8 µm HT HPLC 600 bar Agilent 1200 RRHT-1100 LC Agilent 1100 www.agilent.com/chem/store 57
1100 Agilent 1100 RRHT LC 1100 (VWD) 4.6 x 50 mm 1.8 µm (3) 5188-5323 VWD 5 µl DAD SL DAD (MWD) 4.6 x 50 mm 1.8 µm (2) 5188-5324 DAD 5 µl 2.1 x 50 mm 1.8 µm (2) 5188-5328 DAD 1.7 µl LC/MS : : : : : 50 C : Eclipse XDB-C18 924975-302 3.0 x 30 mm, 1.8 µm A 0.1% FA B 0.075% FA 18 65% B/2 min 65-88% B/3 min 276 nm 2g 10 ml + 10 µl 10 min 0.45 µm 1 µl mau 800 700 600 500 400 300 200 100 0 mau 350 300 250 200 150 100 50 0 mau 120 100 80 60 40 20 0 Naringin confirmed by LCMS Grapefruit Rind 0.5 1 1.5 2 2.5 min Hesperidin confirmed by LCMS Lime Rind 0.5 1 1.5 2 2.5 min Citrus Flavonoids 0.5 1 1.5 2 2.5 min 1. 2. 3. 4. 5. 6. 10-20 58 www.agilent.com/chem/store
20X : : : : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm 827700-902, 827700-902 A:H2O B: ACN 1.2 ml/min, 1.0 ml/min, 2.4 ml/min 0.0 min 50% B 11/1.2/0.4 min 100% B : 40 C, 40 C, 95 C : UV 254 nm : Alkylphenones A 0 B 0 C 2 4 6 8 10 0.2 0.4 0.6 0.8 1 min min 1. C3-Alkylphenone 2. C4-Alkylphenone 3. C5-Alkylphenone 4. C6-Alkylphenone 5. C7-Alkylphenone 6. C8-Alkylphenone 7. C9-Alkylphenone 8. C10-Alkylphenone 9. C12-Alkylphenone 0 0.2 0.4 min : ZORBAX SB-C18 835975-902 4.6 x 50 mm, 3.5 µm : ZORBAX SB-C18 835975-902 4.6 x 50 mm, 3.5 µm (N) 1. 3476 2. 4585 3. 5673 4. 6180 (N) 1. 6560 2. 8958 3. 11508 4. 12266 : : : : 25% 75% 1.5 ml/min 254 nm 1. 2. 3. 4- - 4. 3.5 µm www.agilent.com/chem/store 59
A B C D : : : : Eclipse XDB-C18 990967-902 4.6 x 250 mm, 5 µm Eclipse XDB-C18 963967-902 4.6 x 150 mm, 3.5 µm Eclipse XDB-C18 966967-902 4.6 x 75 mm, 3.5 µm Eclipse XDB-C18 925975-902 4.6 x 50 mm, 1.8 µm 73% : 27% 25 mm ph 7.0 1 ml/min 254 nm 1. 2. 3. 4. 5. 6. 7. A 25 cm 5 µm 11.5 min (3.5 µm) B C HT 2.2 min 80% A B : : : Eclipse XDB-C8 928700-906 2.1 x 100 mm, 1.8 µm Eclipse XDB-C18 961753-902 2.1 x 100 mm, 3.5 µm A B 0.5 ml/min 0.0 min 50% B 20.0 min 100% B : 40 C : UV 254 nm : A B mau 120 100 80 60 40 20 0 0 2 4 6 8 10 12 14 16 18 min mau 100 80 60 40 20 1 1 0 0 2 4 6 8 10 12 14 16 18 min 11 11 1. 2. C3-3. C4-4. C5-5. C6-6. C7-7. C8-8. C9-9. C10-10. C12-11. C14- : SB-C18 827700-902 2.1 x 50 mm, 1.8 µm : A 60% B 40% : 1 ml/min : 80 C : UV 254 nm : QC 60 www.agilent.com/chem/store
(1.8µm) (3.5µm) N (3.5 µm)* N (1.8 µm) 150 21,000 32,500 100 14,000 24,000 75 10,500 17,000** 50 7000 12,000 30 4200 6000 20 3500 15 2100 2500 - N 1/2 * 5 µm HPLC 40% N 4.6 mm ** ZORBAX 600 bar 9000 psi Eclipse Plus C18 (mm) (µm) USP L1 600 bar 4.6 x 150 1.8 959994-902 Eclipse Plus C8 USP L7 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7 Extend- C18 USP L1 600 bar 4.6 x 100 1.8 959964-902 959964-906 928975-902 928975-906 728975-902 600 bar 4.6 x 50 1.8 959941-902 959941-906 927975-902 927975-906 727975-902 600 bar 4.6 x 30 1.8 959931-902 959931-906 924975-902 924975-906 724975-902 600 bar 4.6 x 20 1.8 926975-902 926975-906 726975-902 600 bar 3.0 x 100 1.8 959964-302 959964-306 928975-302 928975-306 728975-302 600 bar 3.0 x 50 1.8 959941-302 959941-306 927975-302 927975-306 727975-302 600 bar 3.0 x 30 1.8 924975-302 924975-306 724975-302 600 bar 3.0 x 20 1.8 926975-302 926975-306 726975-302 600 bar 2.1 x 100 1.8 959764-902 959764-906 928700-902 928700-906 728700-902 600 bar 2.1 x 50 1.8 959741-902 959741-906 927700-902 927700-906 727700-902 600 bar 2.1 x 30 1.8 959731-902 959731-906 924700-902 924700-906 724700-902 600 bar 2.1 x 20 1.8 926700-902 926700-906 726700-902 600 bar 1.0 x 100 1.8 928600-902 928600-906 728600-902 600 bar 1.0 x 50 1.8 922600-902 922600-906 722600-902 www.agilent.com/chem/store 61
ZORBAX 600 bar 9000 psi SB-C18 SB-C8 SB-Phenyl SB-CN (mm) (µm) USP L1 USP L7 USP L11 USP L10 SB-Aq 600 bar 4.6 x 150 1.8 829975-902 829975-906 829975-912 829975-905 600 bar 4.6 x 100 1.8 828975-902 828975-906 828975-912 828975-905 828975-914 600 bar 4.6 x 50 1.8 827975-902 827975-906 827975-912 827975-905 828975-914 600 bar 4.6 x 30 1.8 824975-902 824975-906 824975-912 824975-905 824975-914 600 bar 4.6 x 20 1.8 826975-902 826975-906 600 bar 3.0 x 150 1.8 829975-302 829975-306 829975-312 829975-305 600 bar 3.0 x 100 1.8 828975-302 828975-306 828975-912 828975-305 828975-314 600 bar 3.0 x 50 1.8 827975-302 827975-306 827975-912 827975-305 827975-314 600 bar 3.0 x 30 1.8 824975-302 824975-306 824975-305 600 bar 3.0 x 20 1.8 826975-302 826975-306 600 bar 2.1 x 150 1.8 820700-902 820700-906 820700-912 820700-905 600 bar 2.1 x 100 1.8 828700-902 828700-906 828700-912 828700-905 828700-914 600 bar 2.1 x 50 1.8 827700-902 827700-906 827700-912 827700-905 827700-914 600 bar 2.1 x 30 1.8 824700-902 824700-906 824700-912 824700-905 824700-914 600 bar 2.1 x 20 1.8 826700-902 826700-906 600 bar 1.0 x 100 1.8 828600-902 828600-906 828600-905 62
Zorbax 400 bar 6000 psi XDB-C18 XDB-C8 SB-C18 SB-C8 Extend-C18 (mm) (µm) USP L1 USP L7 USP L1 USP L7 USP L1 4.6 x 50 1.8 922975-902 922975-906 822975-902 822975-906 722975-902 3/ 4.6 x 50 1.8 922975-932 822975-932 2.1 x 50 1.8 922700-902 822700-902 3/ 2.1 x 50 1.8 922700-932 822700-932 ( 820555-901) 4.6 x 50 1.8 925975-902 825975-902 4.6 x 50 1.8 925975-932 825975-932 3/ 2.1 x 50 1.8 925700-902 825700-902 2.1 x 50 1.8 925700-932 825700-932 3/ 4.6 x 30 1.8 923975-902 823975-902 4.6 x 30 1.8 923975-932 823975-932 3/ 2.1 x 30 1.8 923700-902 823700-902 2.1 x 30 1.8 923700-932 823700-932 3/ 4.6 x 15 1.8 921975-902 821975-902 4.6 x 15 1.8 921975-932 821975-932 3/ 2.1 x 15 1.8 921700-902 821700-902 2.1 x 15 1.8 921700-932 821700-932 3/ 820555-901 820555-901 www.agilent.com/chem/store 63
ZORBAX 3.5 µm (4.6 mm) (2.1 mm) 5 µm Agilent ZORBAX 3.5 µm 5 µm 15-150 mm 1-4.6 mmlc/ms 3.5 µm 5 µm ZORBAX A ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm B ZORBAX Bonus-RP 866668-901 4.6 x 75 mm, 3.5 µm Time (min) C ZORBAX Bonus-RP 861700-901 2.1 x 50 mm, 3.5 µm : 80% 25 mm NH 4 Ac ph 5.5 20% : 1.5 ml/min 1.5 ml/min 0.3 ml/min : 40 C : 254 nm Time (min) Time (min) 1. 2. 3. 4. 3.5 µm LC/MS 64 www.agilent.com/chem/store
: ZORBAX SB-C18 866953-902 4.6 x 75 mm, 3.5 µm : A 25 mm NaH 2 PO 4 ph 3.0 B : 2.0 ml/min : 35 C : 254 nm 1. 2. 3. 4. 5. Time (min) Time (min) Time (min) 4.6 x 75 mm 3.5 µm ZORBAX 3.5 µm Eclipse (mm) Eclipse Plus C18 USP L1 Eclipse Plus C8 USP L7 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7 Eclipse XDB-Phenyl USP L11 Eclipse XDB-CN USP L10 (µm) 4.6 x 150 3.5 959963-902 959963-906 963967-902 963967-906 963967-912 963967-905 4.6 x 100 3.5 959961-902 959961-906 961967-902 961967-906 961967-905 4.6 x 75 3.5 959933-902 959933-906 966967-902 966967-906 966967-912 966967-905 4.6 x 50 3.5 959943-902 959943-906 935967-902 935967-906 935967-912 4.6 x 30 3.5 959936-902 959936-906 934967-902 934967-906 4.6 x 20 3.5 932967-902 932967-906 3.0 x 150 3.5 959963-302 959963-306 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 959961-302 959961-306 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 * 2.1 x 150 3.5 959763-902 959763-902 930990-902 930990-906 * 2.1 x 100 3.5 959793-902 959793-906 961753-902 961753-906 961753-905 * 2.1 x 75 3.5 966735-902 * 2.1 x 50 3.5 959743-902 959743-906 971700-902 971700-906 * 2.1 x 30 3.5 959733-902 959733-906 974700-902 974700-906 * 2.1 x 20 3.5 972700-902 972700-906 * 1.0 x 150 3.5 963600-902 963600-906 * 1.0 x 50 3.5 965600-902 965600-906 * 1.0 x 30 3.5 961600-902 961600-906 3/ 1.0 x 17 5 5185-5921 5185-5921 4/ 4.6 x 12.5 5 820950-936 820950-937 820950-925 820950-926 820950-927 820950-935 4/ 2.1 x 12.5 5 821125-936 821125-937 821125-926 821125-926 821125-926 821125-935 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm www.agilent.com/chem/store 65
ZORBAX 3.5 µm StableBond SB-C18 SB-C8 SB-CN SB-Phenyl SB-C3 (mm) (µm) USP L1 USP L7 USP L10 USP L11 USP L56 SB-Aq 4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914 4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914 4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914 4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914 4.6 x 30 3.5 834975-902 834975-906 4.6 x 20 3.5 832975-902 832975-906 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-312 861954-309 861954-314 * 2.1 x 150 3.5 830990-902 830990-906 830990-914 * 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914 * 2.1 x 75 3.5 866735-902 * 2.1 x 50 3.5 871700-902 871700-906 871700-914 * 2.1 x 30 3.5 874700-902 874700-906 * 2.1 x 20 3.5 872700-902 872700-906 * 1.0 x 150 3.5 863600-902 863600-906 * 1.0 x 50 3.5 865600-902 865600-906 * 1.0 x 30 3.5 861600-902 861600-906 1.0 x 17 5 5185-5920 5185-5920 3/ 4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-917 820950-922 820950-933 4/ 2.1 x 12.5 5 821125-926 821125-926 821125-924 821125-926 821125-924 821125-933 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm 66 www.agilent.com/chem/store
ZORBAX 3.5 µm Rx Bonus-RP Extend-C18 *RR 3.5 µm Rx-C18 Rx-C8 Extend-C18 (mm) (µm) USP L1 USP L7 Bonus-RP USP L1 4.6 x 150 3.5 863967-902 863953-906 863668-901 763953-902 4.6 x 100 3.5 861967-902 861953-906 864668-901 764953-902 4.6 x 75 3.5 866967-902 866953-906 866668-901 766953-902 4.6 x 50 3.5 735953-902 3.0 x 150 3.5 863967-302 863954-306 863668-301 763954-302 3.0 x 100 3.5 861967-302 861954-306 864668-301 764953-302 2.1 x 150 3.5 830990-906 863700-901 * 2.1 x 100 3.5 861767-902 861753-906 861768-901 761753-902 * 2.1 x 50 3.5 861700-901 735700-902 * 1.0 x 150 3.5 863608-901 763600-902 * 1.0 x 50 3.5 865608-901 765600-902 * 1.0 x 30 3.5 861608-901 761600-902 3/ 1.0 x 17 5 5185-5922 5185-5923 4/ 4.6 x 12.5 5 820950-914 820950-913 820950-928 820950-930 4/ 2.1 x 12.5 5 821125-915 821125-915 821125-928 821125-930 820888-901 820888-901 820888-901 820888-901 www.agilent.com/chem/store 67
ZORBAX 4.6 mm 60% S/N 2 3 LC/MS LC ZORBAX 3.0 mm 4.6mm 50% LC/MS 0.5 ml/min (APCI) (APPI) MS 4.6 mm 2 3 HPLC 60% A B : : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm ZORBAX SB-C18 883975-302 3.0 x 150 mm, 5 µm 20% : 80% 0.2 M Na2HPO4 + 0.1 M ph 2.6 1. 2. 3. 4. 5. 6. Time (min) Time (min) 4.6 3.0 mm 50% 68 www.agilent.com/chem/store
A B : : ZORBAX SB-C18 863953-902 4.6 x 150 mm, 3.5 µm ZORBAX SB-C18 863954-302 3.0 x 150 mm, 3.5 µm 25% : 75% 0.4% 254 nm 1. 2. 4 5,7-4.6 mm 2-3 HPLC Time (min) LC/MS : ZORBAX SB-C18 861954-302 3.0 x 100 mm, 3.5 µm A 70% + 0.4% B 30% + 0.4% 0.425 ml/min A UV 254 nm B APCI C 1. 2. 3. 4. 5. 6. LC/MS MS APCI 0.5 ml/min Time (min) www.agilent.com/chem/store 69
ZORBAX 80Å StableBond SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl (mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq 3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314 3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-309 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314 ZORBAX 300Å StableBond 300SB-C18 300SB-C8 300SB-CN 300SB-C3 (mm) (µm) USP L1 USP L7 USP L10 USP L56 3.0 x 150 3.5 863974-302 863974-306 863974-309 863974-309 3.0 x 100 3.5 861973-306 ZORBAX 80Å Eclipse XDB XDB-C18 XDB-C8 XDB-Phenyl XDB-CN (mm) (µm) USP L1 USP L7 USP L11 USP L10 3.0 x 250 5 990967-302 990967-306 990967-312 990967-305 3.0 x 150 5 993967-302 993967-306 993967-312 993967-905 3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 ZORBAX 80Å Bonus-RP Rx Rx-C18 Rx-C8 (mm) (µm) Bonus-RP USP L1 USP L7 3.0 x 250 5 880668-301 880967-302 880975-306 3.0 x 150 5 883668-301 883967-302 883975-306 3.0 x 150 3.5 863668-301 863967-302 863954-306 3.0 x 100 3.5 864668-301 861967-302 861954-306 ZORBAX 80Å Extend-C18 Extend-C18 (mm) (µm) USP L1 3.0 x 250 5 770450-302 3.0 x 150 3.5 763954-302 3.0 x 150 5 773450-302 3.0 x 100 3.5 764953-302 3.0 x 50 3.5 735954-302 70 www.agilent.com/chem/store
ZORBAX 1.0 mm LC/MS 1 mm 2.1 mm 5 50 µl/ HPLC 400 bar StableBond SB-C18 SB-C8 300SB-C18 Eclipse XDB-C18 XDB-C8 Bonus RP Extend C-18 Poroshell 1.8 µm ZORBAX 300SB-C18 : ZORBAX 300SB-C18 863630-902 1.0 x 150 mm, 3.5 µm : 60 min 2-60% B A 0.1% B 0.075% /80% : 50 µl/min : 50 C : 215 nm 2 µl rhgh 1.0 mm www.agilent.com/chem/store 71
ZORBAX 1.0 mm SB-C18 SB-C8 300SB-C18 300SB-C8 (mm) (µm) USP L1 USP L7 USP L1 USP L7 1.0 x 250 5 861630-902 * 1.0 x 150 3.5 863600-902 863600-906 863630-902 863630-906 * 1.0 x 50 3.5 865600-902 865600-906 865630-902 865630-906 * 1.0 x 30 3.5 861600-902 861600-906 SB-CN USP L10 ** 1.0 x 50 1.8 822600-902 822600-906 822600-905 3/ 1.0 x 17 5 5185-5920 5185-5920 5185-5920 5185-5920 XDB-C18 XDB-C8 Extend-C18 (mm) (µm) USP L1 USP L7 Bonus-RP USP L1 * 1.0 x 150 3.5 963600-902 963600-906 863608-901 763600-902 * 1.0 x 50 3.5 965600-902 965600-906 865608-901 765600-902 * 1.0 x 30 3.5 961600-902 961600-906 861608-901 761600-902 600 bar** 1.0 x 100 1.8 928600-902 928600-906 728600-902 3/ 1.0 x 17 5 5185-5921 5185-5921 5185-5922 5185-5923 (mm) (µm) Poroshell Poroshell 300SB-C18 300SB-C8 Poroshell 300SB-C3 Poroshell 300Extend-C18 1.0 x 75 5 661750-902 661750-906 661750-909 671750-902 3/ 1.0 x 17 5 5185-5968 5185-5968 5185-5968 *RR 3.5 µm **RRHT 1.8 µm 72 www.agilent.com/chem/store
ZORBAX LC/MS 0.5 0.3 0.1 0.075 mm 80Å 300Å / Agilent ZORBAX 0.5 0.3 mm 0.1 0.075 mm HPLC LC/MS SCX www.agilent.com/chem/store 73
A B C D E F ZORBAX 300SB-C8 5065-4460 0.3 x 150 mm, 3.5 µm ZORBAX Eclipse XDB-C18 5064-8291 0.3 x 150 mm, 5 µm ZORBAX Eclipse XDB-C18 5064-8291 0.3 x 150 mm, 5 µm ZORBAX SB-C18 5064-8255 0.3 x 150 mm, 5 µm ZORBAX 300SB-C18 5064-8267 0.3 x 150 mm, 3.5 µm ZORBAX 300Extend-C18 5065-4464 0.3 x 150 mm, 3.5 µm : + 0.05% ph = 2.2 = A + 0.045% = B 0.5%B/min 0 min = 1%B 60 min = 31%B 70 min = 50%B 75 min = 85%B 80 min = 85%B 81 min = 1%B 110 min = 1%B : 5.5 µl/min 200-500 µl/min : 30 C : 206/10 nm 450/80 nm 0.1 µl 400 300 200 100 0 1. -5 ng/100 nl 2. - -20 ng/100 nl 3. 28 ng/100 nl 4. 20 ng/100 nl 5. II 20 ng/100 nl 1 1 1 1 1 1 2 3 4 5 1 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 0 20 40 60 Time (min) ZORBAX 5 74 www.agilent.com/chem/store
: : : : ZORBAX SB-C18 5064-8255 0.3 x 150 mm, 5 µm ZORBAX SB-C18 5064-8256 0.5 x 150 mm, 5 µm ZORBAX SB-C18 863600-902 1.0 x 150 mm, 3.5 µm ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm 200 ng 0.3 mm 4.6 mm 100 0.1 mm-0.075 mm 2000 Time (min) www.agilent.com/chem/store 75
: ZORBAX SB-C18 5064-8256 0.5 x 150 mm, 5 µm : A 75% 0.4% B 25% 0.4% : 20 µl/min : 25 C : 0.1 µl 1. 2. 3. 4. 5. 6. StableBond-C18 0.5 x 150 mm 5 µm (k) 0.8% RSD (a) 0.4% RSD Time (min) 76
LC/MS ZORBAX HPLC 1100/1200 1. 1100 SCX 1100/1200 1100/1200 LC/MSD 1100/1200 2. 1100 SCX 1100/1200 1100/1200 LC/MSD www.agilent.com/chem/store 77
HPLC 2-D HPLC : : : : : MS : ZORBAX 300SB-C18 5065-9913 0.3 x 5 mm, 5 µm ZORBAX 300SB-C18 5065-9911 0.075 x 150 mm, 3.5 µm : 3% /0.1% : A =, 0.1%, B =, 0.1% : 30 µl/min : 300 nl/min : : 6 min = 3%B, 120 min = 60%B, 125 min = 80%B, 130 min = 80% B, 131 min = 3%B, 140 min = 3% B : Nano ESI, : 5L/min,.: 225 C. : : 1:35 V, : 115 V, 1:12 V, 2:3.5 V, : 80 V. ICC: on, : 4, : 150 ms; 60.000,, MS/MS : 1-8 µl Salt Step Elution: 8ml 10 mm-100 mm KCI ( 10 mm ), 125 mm, 150 mm, 200 mm, 300 mm, 500 mm, 1M. Time (min) 30 mm KCI 60 mm KCI 75 mm KCI 120 mm KCI (BSA) HPLC BSA 78 www.agilent.com/chem/store
ZORBAX Poroshell Eclipse 300SB- 300Extend Bio-SCX (mm) (µm) SB-C18 XDB-C18 C18 300SB-C8 300SB-C8 C18 II 0.8 x 50 3.5 5065-9942 0.5 x 250 5 5064-8258 5064-8286 5064-8266 0.5 x 150 5 5064-8256 5064-8287 5064-8264 0.5 x 75 5 5065-4468 0.5 x 35 5 5064-8254 5064-8296 5064-8294 * 0.5 x 35 3.5 5064-8260 5064-8298 5065-4459 0.3 x 250 5 5064-8257 5064-8269 5064-8265 0.3 x 150 5 5064-8255 5064-8291 5064-8263 0.3 x 35 5 5064-8253 5064-8297 5064-8295 0.3 x 35 3.5 5065-9912 * 0.3 x 150 3.5 5064-8261 5064-8271 5064-8267 5065-4460 5065-4464 * 0.3 x 100 3.5 5064-8259 5065-4461 5065-4465 * 0.3 x 75 3.5 5064-8270 5065-4462 5065-4466 * 0.3 x 50 3.5 5064-8300 5065-4463 5065-4467 10/ 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 *RR 3.5 µm 300SB-C18 (mm) (µm) USP L1 ** 0.1 x 150 3.5 5065-9910 ** 0.075 x 150 3.5 5065-9911 300SB-C8 USP L7 * 0.075 x 50 3.5 5065-9924 5065-9923 /5/ 0.3 x 5 5 5065-9913 5065-9914 / 5065-9915 5065-9915 *RR 3.5 µm www.agilent.com/chem/store 79
ZORBAX PrepHT ZORBAX 2000 mg 5 7 µm 5000 psi/350 bar ZORBAX PrepHT Eclipse XDB StableBond Bonus-RP Extend-C18 ZORBAX PrepHT 5 7 µm ZORBAX 100 ml/min ZORBAX PrepHT 21.2 mm 150 mm 7 µm 80 www.agilent.com/chem/store