Agilent ZORBAX
Agilent ZORBAX HPLC... phzorbax 3 1. 6-9 2. www.agilent.com/chem/lccolumntips 3. 800-820-3278
...2 ZORBAX...5........................................................................ 6 ZORBAX.................................................................. 7 ZORBAX........................................................ 8................................................................ 10 USP................................................................ 12...................................................................... 13.............................................................................. 18 ZORBAX...19 ZORBAX Eclipse Plus......................................................................... 20 ZORBAX Eclipse XDB......................................................................... 26 ZORBAX 80Å StableBond..................................................................... 33 ZORBAX Rx................................................................................. 39 ZORBAX 80Å Extend-C18..................................................................... 41 ZORBAX Bonus-RP.......................................................................... 45 ZORBAX...................................................................... 49 ZORBAX...................................................................... 50 ZORBAX........................................................................ 51 ZORBAX........................................................................ 52...55 ZORBAX 1.8 µm............................................................. 57 ZORBAX 3.5 µm............................................................... 64 ZORBAX.......................................................................... 68 ZORBAX 1.0 mm.............................................................. 71 ZORBAX.................................................................... 73 ZORBAX PrepHT........................................................................... 80............................................................................ 84 Ultron................................................................................ 87 ZORBAX...89 ZORBAX 300Å StableBond.................................................................... 94 ZORBAX 300Å Extend-C18.................................................................... 99 ZORBAX Poroshell................................................................. 102 ZORBAX Eclipse (AAA).......................................................... 105 ZORBAX GF-250 GF-450...................................................... 107 ZORBAX SAX SCX............................................................ 111
ZORBAX Eclipse Plus LC Eclipse Plus 600 bar ZORBAX (RRHT) 1.8 µm LC ZORBAX Eclipse LC 40 2 www.agilent.com/chem/store
Agilent 1200 20 1200 HPLC 40 Agilent 1200 ZORBAX RRHT 1.8 µm www.agilent.com/chem/1200rr LC 60% 600 30 60,000 www.agilent.com/chem/cn 1200 ZORBAX RRHT 1200 HPLC www.agilent.com/chem/store 3
ZORBAX Eclipse ZORBAX Eclipse Eclipse Eclipse Eclipse 1.8 µm 7 µm... ph 1.8 µm 7 µm 600 bar ZORBAX 20 60% 4 www.agilent.com/chem/store
ZORBAX USP Agilemt ZORBAX HT Eclipse Plus USP ph ph www.agilent.com/chem/store 5
2007-2008 LC 52-53 52-53 H 2 O H 2 O 19 >2000 19 19 19 19 >2000 107 111 94 "Practical HPLC Methodology and Applications," Brian A. Bidlingmeyer, John Wiley & Sons, Inc., New York, p. 109 6 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX RP-HPLC Eclipse Plus ph 2-9 1.8 3.5 5 µm QA/QC 20-21 Eclipse XDB StableBond (SB) ph (ph 2-9) 1.8 3.5 µm ph C18 90 C C8 C3 Aq 80 C ph C18 C8 C3 CNAq (TFA) LC/MS (TFA) 1.8 3.5 µm 26 33 ZORBAX Rx Bonus-RP Extend-C18 ph SB Rx-C8 SB-C8 ph ph ph 3.5 µm pka ph ph 11.5 LC/MS ph 3.5 µm 39 45 41 ZORBAX ZORBAX ph SB SB ph 51 ZORBAX ODS Classic ph SB XDB 51 www.agilent.com/chem/store 7
ZORBAX HPLC HPLC ZORBAX MW < 2000 MW > 2000 80-20Å 300Å 80-20Å 300Å (300SB) Eclipse Plus C18 StableBond 300SB-C18 C18 C18 4.6 mm 4.6 mm LC/MS (mm) 4.6 0.1-1.5 mg 0.5-3 ml/min 3.0 150-500ug 0.3-1.5 ml/min + HPLC 2.1 50-120 ug 0.1-0.5 ml/min ++ LC/MS 1.0 10-50 ug 10-100 µl/min ++++ LC/MS 0.5, 0.3 1-10 ug 1-15 µl/min +++++ LC/MS 0.1, 0.075 100-200 ng 200-500 nl/min ++++++ LC/MS 9.4 1-10 mg 5-10 ml/min mg 21.2 20-250 mg 20-60 ml/min 1 8
ZORBAX MW < 2000 MW > 2000 5µm 5µm 5 µm 3.5µm HT 1.8µm 3.5µm 5 µm 60% 1.8 µm 200% 3.5µm Poroshell 5µm 3.5 µm Poroshell 150 mm 150 mm 5 µm 150 mm 250 mm 20-30 (3.5 µm) 75 mm 50 mm 150mm 30-60 (3.5 µm) (50 mm) Eclipse Plus C18 4.6 x 150 mm, 5 µm 959993-902 Eclipse Plus C18 4.6 x 75 mm, 3.5 µm 959933-902 ZORBAX 300SB-C18 4.6 x 150 mm, 5 µm 883995-902 ZORBAX 300SB-C18 4.6 x 50 mm, 3.5 µm 865973-902 600 bar Eclipse Plus C18 4.6 x 50 mm, 1.8 µm 959941-902 ZORBAX Poroshell 300SB-C18 2.1 x 75 mm, 5 µm 660750-902 9
HPLC 5000 (60-100Å) 300Å HPLC 5 µm 3.5 µm 1.8 µm 3.5 µm 1.8 µm 3.5 µm LC 50 mm 1.8 µm RRHT LC LC 400 bar 4.6 x 150 mm 4.6 x 75 mm (4.6 x 250 mm) 2.1 3.0 mm ZORBAX ZORBAX SIL ZOR- BAX Rx-SIL ZORBAX SIL ZORBAX Rx-SIL ZORBAX Rx-SIL Eclipse StableBond ZORBAX SIL 10 www.agilent.com/chem/store
ZORBAX C18 C8 C3 CN C8 C18 C8 ph (THF) 200 nm ph ph ph ph ph 2 4 ph HPLC / www.agilent.com/chem/store 11
USP (USP) USP USP L1 L1 L7 L11 (RRHT) USP USP (µm) (Å) L1 1.5 10 µm 1.8, 3.5, 5, 7 ZORBAX Eclipse Plus C18 ZORBAX Eclipse Plus XDB-C18 ZORBAX StableBond SB-C18 ZORBAX Extend-C18 ZORBAX Rx-C18 ZORBAX ODS ZORBAX ODS Classic L3 5 10 µm ZORBAX Sil ZORBAX Rx-Sil L7 L8 L10 L11 L13 L14 L20 L33 L35 L56 L57 1.5 10 µm 10 µm 3 10 µm 1.5 10 µm 3 10 µm 10 µm 3 10 µm 4,000 400,000 ph 3 10 µm 5 µm 120 ZORBAX Eclipse Plus C8 ZORBAX Eclipse XDB-C8 ZORBAX SB-C8 ZORBAX Rx-C8 ZORBAX C8 3, 5 5 5 5 1.8, 3.5, 5, 7 3.5, 5 3, 5 95 80 80 80 80 70 70 70 80 95 80 80 80 70 ZORBAX NH2 5 70 ZORBAX Eclipse XDB-CN ZORBAX SB-CN ZORBAX CN ZORBAX Eclipse XDB-Phenyl ZORBAX SB-Phenyl ZORBAX 3.5, 5 3.5, 5 3, 5 3.5, 5 1.8, 3.5 5 80 80 70 80 80 70 ZORBAX TMS 5 70 ZORBAX SAX 5 70 ZORBAX GF-250 4 150 ZORBAX GF-250 4 150 ZORBAX GF-250 4 6 150 ZORBAX SB-C3 3.5, 5 80 Ultron ES-OVM 5 120 12 www.agilent.com/chem/store
ZORBAX ph 1-12 ph ph ph ph ph ph 1 ph ph ph ph ph 1 HPLC ph pka ph pka 6.5 ph < 3 - A ph 7 - B ph > 9 - C ph RP-HPLC / ph ph LC/MS ph (TFA) pka ph ph SiOH ph 4-5 SiO TEA Rx-SIL Rx-SIL ph www.agilent.com/chem/store 13
ph (ph 2-3) ph ph 2-3 ph ph ph ph 20-50 mm (ph 2-3) LC/MS ph LC/MS ph ZORBAX Eclipse Plus ZORBAX Eclipse Plus C18 C8 ph Eclipse Plus Eclipse Eclipse Plus ph 2-9 ph 2 14 www.agilent.com/chem/store
ZORBAX ph ph Eclipse Plus Eclipse XDB C18 C8 StableBond SB-C18 SB-C8 SB-Phenyl SB-CN SB-C3 SB-Aq ph ph ph 1-2 StableBond ph www.agilent.com/chem/store 15
ph (4-9) ZORBAX Eclipse Plus ph ph Eclipse Plus C18 ph Eclipse Plus ph 9 ph - ph ph 6-9 ph ph ph Eclipse Plus C18 ph=7 ph 6.1-8.1 ph 3.8-5.8 LC/MS ZORBAX Eclipse XDB-Phenyl CN Bonus-RP ph ph Eclipse XDB-Phenyl Eclipse XDB-CN Bonus-RP Bonus-RP 100% 16 www.agilent.com/chem/store
ZORBAX ph 3 2a Eclipse XDB 60 C Stablebond 80-90 C ph 7 ph 9 1 ZORBAX Eclipse Plus C18 C8 ph 2.5 (2-3) 20-50 mm T=30 C 60 C 0.5 < k < 20 2 5% (v/v) 3 0.5 < k < 20 2 4 Eclipse XDB-C18 C8 SB-CN AQ Bonus-RP 1 5 ZORBAX Eclipse Plus C18 C8 ph 7 (6-9) 20-50 mm T = 30 C 40 C 0.5 < k < 20 6 5% (v/v) 7 0.5 < k < 20 6 8 ZORBAX Eclipse XDB Bonus-RP 5 9 ZORBAX Extend-C18 ph 10.5 (9-11.5) 5-20 mm 10-50 mm T = 25 C 40 C 0.5 < k < 20 ph ph 1a ZORBAX SB-C18 SB-C8 ph 1-3 20-50 mm T = 30 C 90 C 0.5 < k < 20 1c 5% (v/v) 5c 5% (v/v) 1b 10 mm 25-50 mm ph 3 0.5 < k < 20 1d 5% (v/v) 1e 0.5 < k < 20 5a 10 mm 25-50 mm ph 7 0.5 < k < 20 5b 5d 0.5 < k < 20 10a 10 0.5 < k < 20 HPLC 17
HPLC Agilent HPLC 2 3 4 4.6 mm ZORBAX 400 bar PEEK 1/16 LC ZORBAX 3.5 µm 1.8 µm ZORBAX ZORBAX Prep LC/MS LC/MS/MS Eclipse XDB ph 2-9 StableBond ph 2000 psi 135 bar 13.5 MPa 1/16 LC 21.2 30 mm 18 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX ph ZORBAX ZORBAX Eclipse Plus HPLC ZORBAX Eclipse XDB HPLC ZORBAX StableBond HPLC ph ZORBAX Rx HPLC ph 9 ZORBAX Extend-C18 HPLC ph ZORBAX Bonus-RP HPLC - ZORBAX ZORBAX ZORBAX HPLC A www.agilent.com/chem/store 19
ZORBAX Eclipse Plus LC... Eclipse Plus Eclipse Plus Eclipse Eclipse Plus Eclipse Plus 1.8 3.5 5 µm LC LC LC Eclipse 1.8 µm 7 µm 20 www.agilent.com/chem/store
ZORBAX ph HPLC 1.8µm Eclipse Plus ph ZORBAX Eclipse Plus C18 95Å 160 m 2 /g 60 C 2.0-9.0 8% ZORBAX Eclipse Plus C8 95Å 160 m 2 /g 60 C 2.0-8.0 8% www.agilent.com/chem/store 21
Eclipse Plus: : Eclipse Plus C18 Ace 5 C18 SunFireC18 XBridgeC18 Eclipse Plus C18 959996-902 4.6 x 100 mm, 5 µm 1 2 : : : : A 60% B 40% 1.0 ml/min UV 254 nm 5989-4934EN DiscoveryHS C18 LunaC18 (2) GeminiC18 XTerra MS C18 1. 2. 0 0.5 1 1.5 2 2.5 3 3.5 min Eclipse Plus C18 C8 RRHT RR A B Eclipse Plus C18 959941-902 4.6 x 50 mm, 1.8 µm Eclipse Plus C8 959943-906 4.6 x 50 mm, 3.5 µm 1. Oxybenzone 2. Internal Std. 3. Octylmethoxycinnamate : : (30:70) : 2.0 ml/min : 30 C : UV 230 nm 100 C 0.45 µm 1. 2. 3. 247 bar N: 8800 Pw: 0.014 min. TF: 1.20 N: 11600 Pw: 0.047 min. TF: 1.06 N: 11800 Pw: 0.093 min TF: 1.02 0 1 2 3 4 min N: 5000 Pw: 0.019 min. TF: 1.09 N: 6600 Pw: 0.037 min. TF: 0.99 N: 6300 Pw: 0.061 min. TF: 0.98 22 min. 88 bar 0 1 2 3 4 min C8 RRHT C8 22 www.agilent.com/chem/store
ZORBAX ph 2 ph 7 : 1. 2. 3. 4. Eclipse Plus C8 959946-906 4.6 x 50 mm, 5 µm 1 : 3 10-60% B/3 min ph 2.7 A 0.1% B 0.1% fa ph 7.0 A 20 mm B: Excedrin ph 2.7 2 4 0 0.5 1 1.5 2 2.5 3 3.5 m 1 ph 7.0 3 4 2 2 min. 0 0.5 1 1.5 2 2.5 3 3.5 m Eclipse Plus C18 C8 ph Eclipse Plus C8 Eclipse Plus C18 A B Eclipse Plus C8 959996-906 4.6 x 100 mm, 5 µm Eclipse Plus C18 959996-902 4.6 x 100 mm, 5 µm : : : 80% 8mM K 2 HPO 4 ph 7 1.0 ml/min UV 215 nm 0.05 µg/µl 0.5 µl 3.789 Tf=1.04 0 1 2 3 4 5 6 7 8 9 min 5.777 Tf=1.00 0 1 2 3 4 5 6 7 8 9 min C8 C18 Eclipse Plus C8 C18 www.agilent.com/chem/store 23
ZORBAX Eclipse Plus A XBridge C18 4.6 x 150mm 5µm A 1 N = 14000 Tf = 1.25 2 3 4 5 N = 41000 Tf = 1.13 6 B Eclipse Plus C18 959993-902 4.6 x 150 mm, 5 µm 0 2 4 6 8 10 12 min B 1 N = 23000 Tf = 1.01 N = 60000 Tf = 1.00 2 3 4 5 6 0 2 4 6 8 10 12 min : A 0.1% B 0.1% : 1.0 ml/min : 0.0 min 10% B 15 min 30% B : 40 C : UV 254 nm 5989-4934EN 1. 2. 3. 4. 5. 6. Eclipse Plus A Eclipse Plus C18 959941-902 4.6 x 50 mm, 1.8 µm A Tf = 1.00 O O N + Cl - OCH 3 1. : A 50% 8 mm K2HPO4 ph 7 B 50% ACN : 1.0 ml/min : : UV 254 nm 5989-4934EN 0.4 mg/ml 2 ul 0 0.5 OCH 3 1 1.5 2 2.5 min B Eclipse Plus C18 959993-902 4.6 x 150 mm, 5 µm 959993-902 B Tf = 1.06 0 0.5 1 1.5 2 2.5 min 24 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse Plus Eclipse Plus C18 USP L1 Eclipse Plus C8 USP L7 (mm) (µm) 4.6 x 250 5 959990-902 959990-906 4.6 x 150 5 959993-902 959993-906 4.6 x 100 5 959996-902 959996-906 4.6 x 50 5 959946-902 959946-906 4.6 x 150 3.5 959963-902 959963-906 4.6 x 100 3.5 959961-902 959961-906 4.6 x 75 3.5 959933-902 959933-906 4.6 x 50 3.5 959943-902 959943-906 4.6 x 30 3.5 959936-902 959936-906 600 bar 4.6 x 150 1.8 959994-902 600 bar 4.6 x 100 1.8 959964-902 959964-906 600 bar 4.6 x 50 1.8 959941-902 959941-906 600 bar 4.6 x 30 1.8 959931-902 959931-906 3.0 x 150 5 959993-302 959993-306 3.0 x 150 3.5 959963-302 959963-306 3.0 x 100 3.5 959961-302 959961-306 600 bar 3.0 x 100 1.8 959964-302 959964-306 600 bar 3.0 x 50 1.8 959941-302 959941-306 2.1 x 150 5 959701-902 959701-906 2.1 x 50 5 959746-902 959746-906 2.1 x 150 3.5 959763-902 959763-906 2.1 x 100 3.5 959793-902 959793-906 2.1 x 50 3.5 959743-902 959743-906 2.1 x 30 3.5 959733-902 959733-906 600 bar 2.1 x 100 1.8 959764-902 959764-906 600 bar 2.1 x 50 1.8 959741-902 959741-906 600 bar 2.1 x 30 1.8 959731-902 959731-906 4/ 4.6 x 12.5 5 820950-936 820950-937 4/ 2.1 x 12.5 5 821125-936 821125-937 820888-901 820888-901 www.agilent.com/chem/store 25
O CH 3 Si ZORBAX Eclipse XDB O O CH 3 CH 3 Si CH 3 CH 3 Si CH 3 ph (ph 2-9) 1.8 µm 7 µm CH 3 CH 3 O Si CH 3 CH 3 CH 3 O Si CH 3 Eclipse XDB Agilent ZORBAX Eclipse XDB C18 C8 ph (ph 2-9) Eclipse XDB ph (2-3) ph (6-8) ph Eclipse XDB ph 2-9 1.8 3.5 5 7 µm Eclipse XDB C18 ph ZORBAX Eclipse XDB-C18 80Å 180 m 2 /g 60 C 2.0-9.0 10% ZORBAX Eclipse XDB-C8 80Å 180 m 2 /g 60 C 2.0-9.0 7.6% ZORBAX Eclipse XDB- 80Å 180 m 2 /g 60 C 2.0-9.0 7.2% Phenyl ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 60 C 2.0-8.0 4.3% 26 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse XDB ph : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 1.5 ml/min : 40 C A : ph 3.0 75% 25 mm ph 3.0 25% B : ph 7.0 90% 20 mm ph 7.0 10% ACN 1. 2. 3. 4. 5. 1. 2. N- 3. N- ZORBAX Eclipse XDB ph ph 2-9 ph 3 60 C : ZORBAX SB-C8 883975-906 4.6 x 150 mm, 5 µm : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 70% 50 mm NaAc-HCl ph 3.0 30% ACN 65% 35% : 1.0 ml/min : 60 C : ZORBAX SB-C8 P/N 883975-906 ZORBAX Eclipse XDB-C8 P/N 993967-906 Competitive C Eclipse XDB ph ph Eclipse ph 3 SB-C8 60 C ph 3 12,000 (amitriptyline) Eclipse XDB www.agilent.com/chem/store 27
ph 7.0 : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 60% ACN 40% 250 mm ph 7.0 : 1.5 ml/min : 60 C : 1. 2. 3. 4. 5. Rx-Sil sil-gel - ph 7 (60 C) (250 mm)- Eclipse XDB StableBond A Eclipse XDB-C8 966967-906 4.6 x 75 mm, 3.5 µm B ZORBAX Rx/SB-C8 866953-906 4.6 x 75 mm, 3.5 µm : 70% 25 mm NaH2PO4 ph 3.0 30% : 1.0 ml/min : 35 C 1. 2. 3. Time (min) Time (min) Eclipse XDB StableBond 28 www.agilent.com/chem/store
ZORBAX Eclipse XDB A B C Eclipse XDB-Phenyl 963967-912 4.6 x 150 mm, 3.5 µm Eclipse XDB-C8 963967-906 4.6 x 150 mm, 3.5 µm Eclipse XDB-C18 963967-902 4.6 x 150 mm, 3.5 µm : 15% :85% : 1.0 ml/min : 35 C : 1. 2. 3. -O Eclipse XDB C18 C8 Eclipse XDB-Phenyl Eclipse XDB-C8 : Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 85% 25 mm Na 2 HPO 4 ph 7:15% : 1.0 ml/min : 35 C : 1. 2. 3. Cefoxatime 4. 5. Eclipse XDB-C8 A Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 µm 1. 2. 3. 4. 5. 6. B C Eclipse XDB-CN 993967-905 4.6 x 150 mm, 5 µm Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 µm : A. 60:40 : B. 60:40 : C. 77:23 : : 1.0 ml/min : 25 C : Eclipse XDB-C18 Eclipse XDB-CN www.agilent.com/chem/store 29
ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 XDB-Phenyl USP L11 XDB-CN USP L10 (, 400 bar) 9.4 x 250 5 990967-202 990967-206 4.6 x 250 5 990967-902 990967-906 990967-912 990967-905 4.6 x 150 5 993967-902 993967-906 993967-912 993967-905 4.6 x 50 5 946975-902 946975-906 4.6 x 150 3.5 963967-902 963967-906 963967-912 963967-905 4.6 x 100 3.5 961967-902 961967-906 961967-905 4.6 x 75 3.5 966967-902 966967-906 966967-912 966967-905 4.6 x 50 3.5 935967-902 935967-906 935967-912 600 bar 4.6 x 100 1.8 928975-902 928975-906 600 bar 4.6 x 50 1.8 927975-902 927975-906 600 bar 4.6 x 30 1.8 924975-902 924975-906 600 bar 4.6 x 20 1.8 926975-902 926975-906 3.0 x 250 5 990967-302 990967-306 990967-312 990967-305 3.0 x 150 5 993967-302 993967-306 993967-312 993967-305 3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 600 bar 3.0 x 100 1.8 928975-302 928975-306 600 bar 3.0 x 50 1.8 927975-302 927975-306 600 bar 3.0 x 30 1.8 924975-302 924975-306 600 bar 3.0 x 20 1.8 926975-302 926975-306 2.1 x 150 5 993700-902 993700-906 993700-912 993700-905 2.1 x 50 5 960967-902 960967-906 960967-912 960967-905 * 2.1 x 150 3.5 930990-902 930990-906 * 2.1 x 100 3.5 961753-902 961753-906 961753-905 * 2.1 x 75 3.5 966735-902 * 2.1 x 50 3.5 971700-902 971700-906 600 bar** 2.1 x 100 1.8 928700-902 928700-906 600 bar** 2.1 x 50 1.8 927700-902 927700-906 600bar** 2.1 x 30 1.8 924700-902 924700-906 600 bar** 2.1 x 20 1.8 926700-902 926700-906 * 1.0 x 150 3.5 963600-902 963600-906 * 1.0 x 50 3.5 965600-902 965600-906 * 1.0 x 30 3.5 961600-902 961600-906 3/ 1.0 x 17 5 5185-5921 5185-5921 4/ 4.6 x 12.5 5 820950-925 820950-926 820950-927 820950-935 4/ 2.1 x 12.5 5 821125-926 821125-926 821125-926 821125-935 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm **RRHT 1.8 µm 30 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 977250-102 977250-106 PrepHT 21.2 x 150 7 977150-102 977150-106 PrepHT 21.2 x 150 5 970150-902 970150-906 PrepHT 21.2 x 100 5 970100-902 970100-906 PrepHT 21.2 x 50 5 970050-902 970050-906 PrepHT 17 x 7.5 5 820212-925 820212-926 820444-901 820444-901 PrepHT 2/ 820400-901 820400-901 XDB-Phenyl USP L11 XDB-CN USP L10 (mm) ( 5021-1845) 4.6 x 250 5 7995118-585 7995108-585 4.6 x 150 5 7995118-595 7995108-595 4.6 x 75 3.5 7995118-344 7995108-344 3.0 x 75 3.5 7995230-344 10/ 4.0 x 4 5 7995118-504 7995118-504 5021-1845 5021-1845 (, 400 bar) 4.6 x 50 1.8 922975-902 922975-906 3/ 4.6 x 50 1.8 922975-932 (µm) XDB-C18 USP L1 2.1 x 50 1.8 922700-902 3/ 2.1 x 50 1.8 922700-932 XDB-C8 USP L7 31
ZORBAX Eclipse XDB (mm) (µm) XDB-C18 USP L1 XDB-C8 USP L7 ( 820555-901) 4.6 x 30 3.5 933975-902 933975-906 3/ 4.6 x 30 3.5 933975-932 933975-936 4.6 x 15 3.5 931975-902 931975-906 3/ 4.6 x 15 3.5 931975-932 931975-936 2.1 x 30 3.5 973700-902 973700-906 3/ 2.1 x 30 3.5 973700-932 973700-936 2.1 x 15 3.5 975700-902 975700-906 3/ 2.1 x 15 3.5 975700-932 975700-936 4.6 x 50 1.8 925975-902 3/ 4.6 x 50 1.8 925975-932 4.6 x 30 1.8 923975-902 3/ 4.6 x 30 1.8 923975-932 4.6 x 15 1.8 921975-902 3/ 4.6 x 15 1.8 921975-932 2.1 x 50 1.8 925700-902 3/ 2.1 x 50 1.8 925700-932 2.1 x 30 1.8 923700-902 3/ 2.1 x 30 1.8 923700-932 2.1 x 15 1.8 921700-902 3/ 2.1 x 15 1.8 921700-932 820555-901 820555-901 0.5 x 250 5 5064-8286 0.5 x 150 5 5064-8287 0.5 x 150 3.5 5064-8288 0.5 x 35 3.5 5064-8298 0.3 x 250 5 5064-8269 0.3 x 150 5 5064-8291 0.3 x 150 3.5 5064-8271 0.5 x 35 5 5064-8296 0.3 x 35 5 5064-8297 32 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å StableBond ph ph 1 ph SB-C18 SB-C8 SB-CN SB-Phenyl SB-C3 SB- Aq B Agilent ZORBAX StableBond (SB-C18) SB-C8 SB-C3 SB-Phenyl SB-CN SB-Aq ph StableBond StableBond ph ZORBAX StableBond O OH O OH R Si R R Si R R R 1 R 1 * ph * ZORBAX 80Å 180 m 2 /g 90 C 1.0-8.0 10% SB-C18 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 5.5% SB-C8 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 4% SB-C3 ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 5.5% SB-Phenyl ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 4% SB-CN ZORBAX 80Å 180 m 2 /g 80 C 1.0-8.0 SB-Aq *StableBond ph ph 6-8 <40 C 0.01-0.02 M ph Eclipse XDB Bonus-RP O Si R StableBond R 1 www.agilent.com/chem/store 33
StableBond SB-C18 ph ph 0.8 90 C : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm : ZORBAX Rx-C18 883967-902 4.6 x 150 mm, 5 µm : 50% /50% 1.0% : 90 C Relative Retention Stablebond SB-C18 -C18 ZORBAX Rx-C18 -C18 A-C18 B-C18 C-C18 D-C18 E-C18 Column Volumes of Mobile Phase Purge PH (0.8) (90 C) StableBond SB-C18 ZORBAX Rx-C18 StableBond SB-C18 ZORBAX SB-CN ph (ph 2.0 50 C) : ZORBAX SB-CN 883975-905 4.6 x 150 mm, 5 µm % k Remaining ZORBAX StableBond SB-CN StableBond ph CN Column Volumes SB-CN A ZORBAX SB-C18 866953-902 4.6 x 75 mm, 3.5 µm B ZORBAX SB-CN 866953-905 4.6 x 75 mm, 3.5 µm : 30% 70% 25mM NaH 2 PO 4 ph 2.5 : 1.0 ml/min : 35 C 1. 2. 3. 4. 5. Time (min) Time (min) SB-CN 50% 34 www.agilent.com/chem/store
ZORBAX RRHT mau 450 400 350 300 250 200 150 100 50 0 0 1 2 1 1 2 1 2 3 2 4 3 5 4 3 3 1 2 3 4 5 : (40%): 60% ph=2.4 25mM NaH 2 PO 4 : 1.85 ml/min 5 3, 4 & 5 1. 2. 3. 4. 5. 4 5 5 4 A SB-C18 827975-902 4.6 x 50 mm, 1.8 µm B SB-C8 827975-906 4.6 x 50 mm, 1.8 µm C ZORBAX SB-Phenyl 827975-912 4.6 x 50 mm, 1.8 µm D ZORBAX SB-CN 827975-905 4.6 x 50 mm, 1.8 µm min DAD 254 nm LC Agilent 1200 RR LC 1 µl StableBond 5 SB-Aq A ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm D ZORBAX SB-Phenyl 883975-912 4.6 x 150 mm, 5 µm B ZORBAX SB-C8 883975-906 4.6 x 150 mm, 5 µm E ZORBAX SB-CN 883975-905 4.6 x 150 mm, 5 µm C ZORBAX SB-C3 883975-909 4.6 x 150 mm, 5 µm SB-C3 StableBond SB-C3 Rx-SIL : 18.8 min 0-100% B A 50 mm NaH 2 PO 4 ph 2.5 95% / 5% B 50 mm NaH 2 PO 4 ph 2.5 47% / 53% : 1.0 ml/min : 26 C : : 254 nm 1. 2. 3. d- 4. 5. www.agilent.com/chem/store 35
ZORBAX 80Å StableBond (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 SB-CN USP L10 SB-C3 USP L56 SB-Phenyl USP L11 SB-Aq (, 400 bar) 9.4 x 250 5 880975-202 880967-201 880975-205 880975-209 880975-212 9.4 x 150 5 883975-202 9.4 x 100 5 884975-202 9.4 x 50 5 846975-202 4.6 x 250 5 880975-902 880975-906 880975-905 880975-909 880975-912 880975-914 4.6 x 150 5 883975-902 883975-906 883975-905 883975-909 883975-912 883975-914 4.6 x 50 5 846975-902 846975-906 846975-914 4.6 x 250 3.5 884950-567 4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914 4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914 4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914 4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914 4.6 x 150 1.8 829975-902 829975-906 829975-905 829975-912 600 bar 4.6 x 100 1.8 828975-902 828975-906 828975-905 828975-912 828975-914 600 bar 4.6 x 50 1.8 827975-902 827975-906 827975-905 827975-912 827975-914 600 bar 4.6 x 30 1.8 824975-902 824975-906 824975-905 824975-912 824975-914 600 bar 4.6 x 20 1.8 826975-902 826975-906 600 bar 3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314 3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314 3.0 x 150 1.8 829975-302 829975-306 829975-305 829975-312 600 bar 3.0 x 100 1.8 828975-302 828975-306 828975-305 828975-312 828975-314 600 bar 3.0 x 50 1.8 827975-302 827975-306 827975-305 827975-312 827975-314 600 bar 3.0 x 30 1.8 824975-302 824975-306 824975-305 600 bar 3.0 x 20 1.8 826975-302 826975-306 600 bar 2.1 x 150 5 883700-922 883700-906 883700-905 883700-909 883700-912 2.1 x 50 5 860975-902 860975-906 860975-905 860975-909 860975-912 860975-914 * 2.1 x 150 3.5 830990-902 830990-906 830990-914 * 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914 * 2.1 x 75 3.5 866735-902 * 2.1 x 50 3.5 871700-902 871700-906 871700-914 *RR 3.5 µm **RRHT 1.8 µm 36 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å StableBond SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl (mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq 2.1 x 150 1.8 820700-902 820700-906 820700-905 820700-912 600 bar** 2.1 x 100 1.8 828700-902 828700-906 828700-905 828700-912 828700-914 600 bar** 2.1 x 50 1.8 827700-902 827700-906 827700-905 827700-912 827700-914 600 bar** 2.1 x 30 1.8 824700-902 824700-906 824700-905 824700-912 824700-914 600 bar** 2.1 x 20 1.8 826700-902 826700-906 600 bar** * 1.0 x 150 3.5 863600-902 863600-906 863600-905 * 1.0 x 50 3.5 865600-902 865600-906 * 1.0 x 30 3.5 861600-902 861600-906 1.0 x 17 5 5185-5920 5185-5920 3/ 2/ 9.4 x 15 7 820675-115 820675-115 820675-124 820675-115 4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-922 820950-917 820950-933 4/ 2.1 x 12.5 5 821125-915 821125-915 821125-924 821125-924 821125-915 821125-933 9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877250-102 877250-106 877250-105 877250-112 877250-114 PrepHT 21.2 x 150 7 877150-102 877150-106 877150-114 PrepHT 21.2 x 150 5 870150-902 870150-906 870150-914 PrepHT 21.2 x 100 5 870100-902 870100-906 870100-914 PrepHT 21.2 x 50 5 870050-902 870050-906 870050-914 PrepHT 17 x 7.5 5 820212-920 820212-915 820212-915 820212-915 820212-933 2/ 820444-901 820444-901 820444-901 820444-901 820444-901 820444-901 PrepHT 2/ *RR 3.5 µm **RRHT 1.8 µm 820400-901 820400-901 820400-901 820400-901 820400-901 820400-901 ( 5021-1845) 4.6 x 250 5 7995218-585 7995208-585 (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 4.6 x 150 5 7995218-595 7995208-595 4.6 x 75 3.5 7995218-344 7995208-344 10/ 4.0 x 4 5 7995118-504 7995118-504 5021-1845 5021-1845 www.agilent.com/chem/store 37
ZORBAX 80Å StableBond (mm) (µm) SB-C18 USP L1 SB-C8 USP L7 (, 400 bar) 4.6 x 50 1.8 822975-902 822975-906 3/ 4.6 x 50 1.8 822975-932 2.1 x 50 1.8 822700-902 3/ 2.1 x 50 1.8 822700-932 ( 820555-901) 4.6 x 30 3.5 833975-902 833975-906 3/ 4.6 x 30 3.5 833975-932 833975-936 4.6 x 15 3.5 831975-902 831975-906 3/ 4.6 x 15 3.5 831975-932 831975-936 2.1 x 30 3.5 873700-902 873700-906 3/ 2.1 x 30 3.5 873700-932 873700-936 2.1 x 15 3.5 875700-902 875700-906 3/ 2.1 x 15 3.5 875700-932 875700-936 4.6 x 50 1.8 825975-902 3/ 4.6 x 50 1.8 825975-932 4.6 x 30 1.8 823975-902 3/ 4.6 x 30 1.8 823975-932 4.6 x 15 1.8 821975-902 3/ 4.6 x 15 1.8 821975-932 2.1 x 50 1.8 825700-902 3/ 2.1 x 50 1.8 825700-932 2.1 x 30 1.8 823700-902 3/ 2.1 x 30 1.8 823700-932 2.1 x 15 1.8 821700-902 3/ 2.1 x 15 1.8 821700-932 820555-901 820555-901 0.5 x 250 5 5064-8258 0.5 x 150 5 5064-8256 0.5 x 35 5 5064-8254 * 0.5 x 150 3.5 5064-8262 0.5 x 35 3.5 5064-8260 0.3 x 250 5 5064-8257 0.3 x 150 5 5064-8255 0.3 x 35 5 5064-8253 0.3 x 150 3.5 5064-8261 *RR 3.5 µm **RRHT 1.8 µm 38 www.agilent.com/chem/store
ZORBAX ZORBAX Rx Eclipse XDB-C18 StableBond SB-C18 Rx-C18 ph StableBond SB-C18 12% 10% ph Rx-C18 Rx-C18 ph 9 Rx-C8 SB-C8 ph ZORBAX Rx-C18 80Å 180 m 2 /g 60 C 2.0-9.0 12% ZORBAX Rx-C8 80Å 180 m 2 /g 60 C 1.0-8.0 5.5% Rx-C18 : : : ZORBAX Rx-C18 880967-302 3.0 x 250 mm, 5 µm 35% :65% 0.5 ml/min 1. 2. Rx-C18 (USP) 4.6 x 250 mm 3.0 mm Rx- C18 60% www.agilent.com/chem/store 39
ZORBAX Rx Rx-C18 Rx-C8 (mm) (µm) USP L1 USP L7** 9.4 x 250 5 880967-202 880967-201 4.6 x 250 5 880967-902 880967-901 4.6 x 150 5 883967-902 883967-901 4.6 x 150 3.5 863967-902 863953-906 4.6 x 100 3.5 861967-902 861953-906 4.6 x 75 3.5 866967-902 866953-906 3.0 x 250 5 880967-302 880975-306 3.0 x 150 5 883967-302 883975-306 3.0 x 150 3.5 863967-302 863954-306 3.0 x 100 3.5 861967-302 861954-306 2.1 x 150 5 883700-902 883700-906 * 2.1 x 100 3.5 861767-902 861753-906 2/ 9.4 x 15 7 820675-115 820675-115 4/ 4.6 x 12.5 5 820950-914 820950-913 4/ 2.1 x 12.5 5 821125-915 821125-915 9.4 x 15 840140-901 840140-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877967-102 877250-106 PrepHT 21.2 x 150 7 877150-106 PrepHT 21.2 x 150 5 870150-906 PrepHT 21.2 x 100 5 870100-906 PrepHT 21.2 x 50 5 870050-906 PrepHT 2/ 820212-914 820212-915 820444-901 820444-901 PrepHT 2/ 820400-901 820400-901 *RR 3.5 µm **Rx-C8 SB-C8 40 www.agilent.com/chem/store
ZORBAX ZORBAX 80Å Extend-C18 ph ph 11.5 ph LC/MS O Si C18 ZORBAX Extend-C18 C18-C18 ph ph ph ph ph ph 10.5 LC/MS ph Extend-C18 ph 2-11.5 Extend-C18 ph * ph ZORBAX Extend-C18 80Å 180 m 2 /g 60 C 2.0-11.5 12.5% * ph 8 60 C ph 8-11.5 40 C O Si C18 Silica Support Extend C-18 C18-C18 www.agilent.com/chem/store 41
Extend-C18 ph : : : : : : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm ph 7 30% 20 mm Na2HPO4 70% ph 11 30% 20 mm 70% 1.0 ml/min 254 nm 1. 2. 3. 4. 5. 6. 7. ph ph Extend-C18 ph Time (min) Time (min) Extend-C18 ph : : : : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm 20% 80% 0.1 M ph 10.0 1.0 ml/min ph W ZORBAX Extend-C18 ph Amount of Dissolved Silica (mg) ZORBAX Extend-C18 W Volume Eluent (L) 42 www.agilent.com/chem/store
ZORBAX Extend-C18 ph : ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm : 80% 25 mm NaH2PO4 ph 3.0 20% : 1.0 ml/min : 35 C : 254 nm : 1. 2. 3. 4. Extend-C18 ph Extend-C18 ph ph Time (min) ZORBAX 80Å Extend-C18 (mm) (, 400 bar) 4.6 x 250 5 770450-902 4.6 x 150 5 773450-902 4.6 x 50 5 746450-902 4.6 x 150 3.5 763953-902 4.6 x 100 3.5 764953-902 4.6 x 75 3.5 766953-902 4.6 x 50 3.5 735953-902 600 bar 4.6 x 100 1.8 728975-902 600 bar 4.6 x 50 1.8 727975-902 400 bar 4.6 x 50 1.8 722975-902 600 bar 4.6 x 30 1.8 724975-902 600 bar 4.6 x 20 1.8 726975-902 3.0 x 250 5 770450-302 3.0 x 150 5 773450-302 3.0 x 150 3.5 763954-302 3.0 x 100 3.5 764953-302 3.0 x 50 3.5 735954-302 600 bar 3.0 x 100 1.8 728975-302 600 bar 3.0 x 50 1.8 727975-302 600 bar 3.0 x 30 1.8 724975-302 600 bar 3.0 x 20 1.8 726975-302 (µm) www.agilent.com/chem/store 43
ZORBAX 80Å Extend-C18 (mm) (µm) 2.1 x 150 5 773700-902 2.1 x 50 5 760450-902 * 2.1 x 100 3.5 761753-902 * 2.1 x 50 3.5 735700-902 600 bar** 2.1 x 100 1.8 728700-902 600 bar** 2.1 x 50 1.8 727700-902 600 bar** 2.1 x 30 1.8 724700-902 600 bar** 2.1 x 20 1.8 726700-902 * 1.0 x 150 3.5 763600-902 * 1.0 x 50 3.5 765600-902 * 1.0 x 30 3.5 761600-902 3/ 1.0 x 17 5 5185-5923 4/ 4.6 x 12.5 5 820950-930 4/ 2.1 x 12.5 5 821125-930 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 150 5 770150-902 PrepHT 21.2 x 100 5 770100-902 PrepHT 21.2 x 50 5 770050-902 PrepHT 2/ 820400-901 PrepHT 2/ 17 x 7.5 5 820212-930 820444-901 *RR 3.5 µm **RRHT 1.8 µm 44 www.agilent.com/chem/store
ZORBAX ZORBAX Bonus-RP ph ph 100% O CH 3 R 1 Si R 1 PG R Agilent ZORBAX Bonus-RP ph Bonus-RP C18 C8 Si CH 3 CH 3 R 1 O Si R 1 PG R CH 3 * ph ZORBAX Bonus-RP 80Å 180 m 2 /g 60 C 2.0-9.0 9.5% * ph 8 60 C ph 8-9 40 C Si CH 3 CH 3 R 1 O Si R 1 PG R Bonus-RP ZORBAX Bonus-RP A ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm B Eclipse XDB-C8 993967-906 4.6 x 150 mm, 5 µm : 75% 25 mm ph 6 25% : 1.0 ml/min : : 254 nm : 3 µl 1. 2. 3. 4. Time (min) Bonus-RP www.agilent.com/chem/store 45
Bonus-RP : Alkye-C8 4.6 x 150 mm 5 µm : 75% 25 mm NH 4 Ac ph 5.5 25% : 1.5 ml/min : 40 C : 254 nm 1. 2. 3. C8 Bonus-RP ph Bonus-RP TF 1. 1.8 2. 1.7 3. 2.1 Time (min) Bonus-RP 2 : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm : 80% 25 mm NH 4 Ac ph 5.5 20% : 1.5 ml/min : 40 C : 254 nm 1. 2. 3. TF 1. 1.1 2. 1.1 3. 1.1 Time (min) 46 www.agilent.com/chem/store
ZORBAX ph ph ZORBAX Bonus-RP : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm : 60% 25 mm ph 7.0:40% ACN : 1.5 ml/min : 23 C Toluene Plate Height, cm / Bonus-RP Column Volume of Purge Bonus-RP ph 7 10,000 -C18/ Bonus-RP : : : : ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm 50% 50% 0.1% 80% 20% 1.0 ml/min 60 C 23 C Toluene Plate Height, cm -C18/ Bonus-RP Column Volume of Purge ph www.agilent.com/chem/store 47
ZORBAX Bonus-RP (mm) (, 400 bar) 4.6 x 250 5 880668-901 4.6 x 150 5 883668-901 4.6 x 150 3.5 863668-901 4.6 x 100 3.5 864668-901 4.6 x 75 3.5 866668-901 3.0 x 250 5 880668-301 3.0 x 150 5 883668-301 3.0 x 150 3.5 863668-301 3.0 x 100 3.5 864668-301 2.1 x 150 5 883725-901 2.1 x 50 5 861971-901 * 2.1 x 150 3.5 863700-901 * 2.1 x 100 3.5 861768-901 * 2.1 x 50 3.5 861700-901 * 1.0 x 150 3.5 863608-901 * 1.0 x 50 3.5 865608-901 * 1.0 x 30 3.5 861608-901 3/ 1.0 x 17 5 5185-5922 4/ 4.6 x 12.5 5 820950-928 4/ 2.1 x 12.5 5 821125-928 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 878250-101 *RR 3.5 µm (µm) PrepHT 21.2 x 150 7 878150-101 PrepHT 21.2 x 150 5 868150-901 PrepHT 21.2 x 100 5 868100-901 PrepHT 21.2 x 50 5 868050-901 PrepHT 2/ 820400-901 PrepHT 2/ 17 x 7.5 5 820212-928 820444-901 48 www.agilent.com/chem/store
ZORBAX ZORBAX ph 2-9 Eclipse ph StableBond ph (1-11.5) ph ph ZORBAX StableBond 4.6 x 150 mm 5 µm SB-C18 SB-CN SB-Phenyl StableBond 4.6 x 75 mm 3.5 µm SB-C18 SB-CN Eclipse XDB 4.6 x 150 mm 5 µm XDB-C18 XDB-C8 XDB-Phenyl Eclipse XDB 4.6 x 75 mm 3.5 µm XDB-C18 XDB-C8 ph 4.6 x 150 mm 5 µm SB-C18 XDB-C18 Extend-C18 ph 4.6 x 75 mm 3.5 µm SB-C18 XDB-C18 Extend-C18 4.6 x 150 mm 5 µm SB-Aq Bonus RP SB-C18 4.6 x 75 mm 3.5 µm SB-Aq Bonus RP SB-C18 5183-4624 5183-4625 5183-4626 5183-4627 5185-5807 5185-5808 5185-5809 5185-5810 www.agilent.com/chem/store 49
ZORBAX ZORBAX HPLC www.agilent.com/chem/lccustom 1-2 1 899999-888 2 3 4 5 (302) 993-5354 custom_columns@agilent.com 1-2 3 50 www.agilent.com/chem/store
ZORBAX ZORBAX ZORBAX A (mm) (µm) ODS (C18) USP L1 C8 USP L7 USP L11 USP L10 TMS USP L13 (, 400 bar) 9.4 x 250 5 880952-202 880952-206 4.6 x 250 5 880952-702 880952-706 880952-712 884950-507 880952-710 4.6 x 250 5 884950-543 4.6 x 150 5 883952-702 883952-706 883952-712 884950-526 883952-710 3.0 x 250 5 880952-302 3.0 x 150 5 883952-302 ( ) 2/ 9.4 x 15 7 820675-115 820675-115 820675-115 820675-124 4/ 4.6 x 12.5 5 820950-902 820950-906 820950-912 820950-905 820950-924 9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877952-102 877952-106 877952-105 PrepHT 2/ 820400-901 820400-901 820400-901 www.agilent.com/chem/store 51
Zorbax ZORBAX Rx-SIL (>99.995%) ZORBAX-SIL ZORBAX Rx-SIL HILIC ZORBAX Eclipse XDB-CN Rx-SIL ZORBAX Rx-SIL ZORBAX CN ZORBAX SIL ZORBAX SIL ZORBAX NH2 - ZORBAX SIL HPLC A D 52 www.agilent.com/chem/store
ZORBAX ph ZORBAX Rx-SIL 80Å 180 m 2 /g 0-8.0 ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 2.0-8.0 4.3% ZORBAX SIL 70Å 300 m 2 /g 0-8.0 ZORBAX CN 70Å 300 m 2 /g 2.0-7.0 7% ZORBAX NH 2 70Å 300 m 2 /g 2.0-7.0 4% ZORBAX CN ZORBAX CN : ZORBAX CN 880952-705 4.6 x 250 mm, 5 µm : A B 2- / (50/50) : 2 ml/min : 10 min. B 2-20% 20% : 50 C : 278 nm (n = 10) 0 5 10 15 20 Time (min) www.agilent.com/chem/store 53
Zorbax (mm) (µm) XDB-CN USP L10 Rx-SIL (, 400 bar) 9.4 x 250 5 880975-201 880952-201 880952-205 880952-208 4.6 x 250 5 880975-901 880952-701 880952-705 880952-708 840300-908 990967-905* 4.6 x 150 5 883975-901 883952-701 883952-705 883952-708 843300-908 993967-905* 2.1 x 150 5 883700-901 993700-905* 2.1 x 50 5 860700-708 ( ) 2/ 9.4 x 15 5 820675-119 820675-119 820675-111 820675-111 4/ 4/ 4.6 x 12.5 5 820950-919 820950-901 820950-905 820950-908 820950-908 820950-935 2.1 x 12.5 5 821125-919 821125-935 9.4 x 15 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 877250-101 877952-101 PrepHT 21.2 x 250 7 877952-105 877952-108 PrepHT 2/ 820400-901 820400-901 820400-901 820400-901 PrepHT 2/ 17 x 7.5 5 820212-919 820444-901 * 54 www.agilent.com/chem/store
ZORBAX (3.5 µm) (1.8 µm) Eclipse XDB StableBond Eclipse Plus RR RRHT ZORBAX 4.6 mm 50% HPLC LC/MS ZORBAX HPLC 2.1 mm 5 ZORBAX ZORBAX Ultron www.agilent.com/chem/store 55
ZORBAX (RR) ZORBAX 600 bar RRHT Agilent 1200 95% 1.8 µm ZORBAX LC RRHT LC, (RR) (RRHT) ZORBAX 1.8 µm Agilent 1200...... 20 100 HPLC 30-40% 56 www.agilent.com/chem/store
ZORBAX 1.8 µm 1.8 µm [600 bar] 2 25% 95% HPLC 80 50 mm ZORBAX (1.8 µm) 1.8 µm 250 mm 95% 80 RRHT ZORBAX Eclipse Plus ZORBAX (Eclipse XDB StableBond Extend) 3.5 µm 1.8 µm HT HPLC 600 bar Agilent 1200 RRHT-1100 LC Agilent 1100 www.agilent.com/chem/store 57
1100 Agilent 1100 RRHT LC 1100 (VWD) 4.6 x 50 mm 1.8 µm (3) 5188-5323 VWD 5 µl DAD SL DAD (MWD) 4.6 x 50 mm 1.8 µm (2) 5188-5324 DAD 5 µl 2.1 x 50 mm 1.8 µm (2) 5188-5328 DAD 1.7 µl LC/MS : : : : : 50 C : Eclipse XDB-C18 924975-302 3.0 x 30 mm, 1.8 µm A 0.1% FA B 0.075% FA 18 65% B/2 min 65-88% B/3 min 276 nm 2g 10 ml + 10 µl 10 min 0.45 µm 1 µl mau 800 700 600 500 400 300 200 100 0 mau 350 300 250 200 150 100 50 0 mau 120 100 80 60 40 20 0 Naringin confirmed by LCMS Grapefruit Rind 0.5 1 1.5 2 2.5 min Hesperidin confirmed by LCMS Lime Rind 0.5 1 1.5 2 2.5 min Citrus Flavonoids 0.5 1 1.5 2 2.5 min 1. 2. 3. 4. 5. 6. 10-20 58 www.agilent.com/chem/store
20X : : : : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm 827700-902, 827700-902 A:H2O B: ACN 1.2 ml/min, 1.0 ml/min, 2.4 ml/min 0.0 min 50% B 11/1.2/0.4 min 100% B : 40 C, 40 C, 95 C : UV 254 nm : Alkylphenones A 0 B 0 C 2 4 6 8 10 0.2 0.4 0.6 0.8 1 min min 1. C3-Alkylphenone 2. C4-Alkylphenone 3. C5-Alkylphenone 4. C6-Alkylphenone 5. C7-Alkylphenone 6. C8-Alkylphenone 7. C9-Alkylphenone 8. C10-Alkylphenone 9. C12-Alkylphenone 0 0.2 0.4 min : ZORBAX SB-C18 835975-902 4.6 x 50 mm, 3.5 µm : ZORBAX SB-C18 835975-902 4.6 x 50 mm, 3.5 µm (N) 1. 3476 2. 4585 3. 5673 4. 6180 (N) 1. 6560 2. 8958 3. 11508 4. 12266 : : : : 25% 75% 1.5 ml/min 254 nm 1. 2. 3. 4- - 4. 3.5 µm www.agilent.com/chem/store 59
A B C D : : : : Eclipse XDB-C18 990967-902 4.6 x 250 mm, 5 µm Eclipse XDB-C18 963967-902 4.6 x 150 mm, 3.5 µm Eclipse XDB-C18 966967-902 4.6 x 75 mm, 3.5 µm Eclipse XDB-C18 925975-902 4.6 x 50 mm, 1.8 µm 73% : 27% 25 mm ph 7.0 1 ml/min 254 nm 1. 2. 3. 4. 5. 6. 7. A 25 cm 5 µm 11.5 min (3.5 µm) B C HT 2.2 min 80% A B : : : Eclipse XDB-C8 928700-906 2.1 x 100 mm, 1.8 µm Eclipse XDB-C18 961753-902 2.1 x 100 mm, 3.5 µm A B 0.5 ml/min 0.0 min 50% B 20.0 min 100% B : 40 C : UV 254 nm : A B mau 120 100 80 60 40 20 0 0 2 4 6 8 10 12 14 16 18 min mau 100 80 60 40 20 1 1 0 0 2 4 6 8 10 12 14 16 18 min 11 11 1. 2. C3-3. C4-4. C5-5. C6-6. C7-7. C8-8. C9-9. C10-10. C12-11. C14- : SB-C18 827700-902 2.1 x 50 mm, 1.8 µm : A 60% B 40% : 1 ml/min : 80 C : UV 254 nm : QC 60 www.agilent.com/chem/store
(1.8µm) (3.5µm) N (3.5 µm)* N (1.8 µm) 150 21,000 32,500 100 14,000 24,000 75 10,500 17,000** 50 7000 12,000 30 4200 6000 20 3500 15 2100 2500 - N 1/2 * 5 µm HPLC 40% N 4.6 mm ** ZORBAX 600 bar 9000 psi Eclipse Plus C18 (mm) (µm) USP L1 600 bar 4.6 x 150 1.8 959994-902 Eclipse Plus C8 USP L7 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7 Extend- C18 USP L1 600 bar 4.6 x 100 1.8 959964-902 959964-906 928975-902 928975-906 728975-902 600 bar 4.6 x 50 1.8 959941-902 959941-906 927975-902 927975-906 727975-902 600 bar 4.6 x 30 1.8 959931-902 959931-906 924975-902 924975-906 724975-902 600 bar 4.6 x 20 1.8 926975-902 926975-906 726975-902 600 bar 3.0 x 100 1.8 959964-302 959964-306 928975-302 928975-306 728975-302 600 bar 3.0 x 50 1.8 959941-302 959941-306 927975-302 927975-306 727975-302 600 bar 3.0 x 30 1.8 924975-302 924975-306 724975-302 600 bar 3.0 x 20 1.8 926975-302 926975-306 726975-302 600 bar 2.1 x 100 1.8 959764-902 959764-906 928700-902 928700-906 728700-902 600 bar 2.1 x 50 1.8 959741-902 959741-906 927700-902 927700-906 727700-902 600 bar 2.1 x 30 1.8 959731-902 959731-906 924700-902 924700-906 724700-902 600 bar 2.1 x 20 1.8 926700-902 926700-906 726700-902 600 bar 1.0 x 100 1.8 928600-902 928600-906 728600-902 600 bar 1.0 x 50 1.8 922600-902 922600-906 722600-902 www.agilent.com/chem/store 61
ZORBAX 600 bar 9000 psi SB-C18 SB-C8 SB-Phenyl SB-CN (mm) (µm) USP L1 USP L7 USP L11 USP L10 SB-Aq 600 bar 4.6 x 150 1.8 829975-902 829975-906 829975-912 829975-905 600 bar 4.6 x 100 1.8 828975-902 828975-906 828975-912 828975-905 828975-914 600 bar 4.6 x 50 1.8 827975-902 827975-906 827975-912 827975-905 828975-914 600 bar 4.6 x 30 1.8 824975-902 824975-906 824975-912 824975-905 824975-914 600 bar 4.6 x 20 1.8 826975-902 826975-906 600 bar 3.0 x 150 1.8 829975-302 829975-306 829975-312 829975-305 600 bar 3.0 x 100 1.8 828975-302 828975-306 828975-912 828975-305 828975-314 600 bar 3.0 x 50 1.8 827975-302 827975-306 827975-912 827975-305 827975-314 600 bar 3.0 x 30 1.8 824975-302 824975-306 824975-305 600 bar 3.0 x 20 1.8 826975-302 826975-306 600 bar 2.1 x 150 1.8 820700-902 820700-906 820700-912 820700-905 600 bar 2.1 x 100 1.8 828700-902 828700-906 828700-912 828700-905 828700-914 600 bar 2.1 x 50 1.8 827700-902 827700-906 827700-912 827700-905 827700-914 600 bar 2.1 x 30 1.8 824700-902 824700-906 824700-912 824700-905 824700-914 600 bar 2.1 x 20 1.8 826700-902 826700-906 600 bar 1.0 x 100 1.8 828600-902 828600-906 828600-905 62
Zorbax 400 bar 6000 psi XDB-C18 XDB-C8 SB-C18 SB-C8 Extend-C18 (mm) (µm) USP L1 USP L7 USP L1 USP L7 USP L1 4.6 x 50 1.8 922975-902 922975-906 822975-902 822975-906 722975-902 3/ 4.6 x 50 1.8 922975-932 822975-932 2.1 x 50 1.8 922700-902 822700-902 3/ 2.1 x 50 1.8 922700-932 822700-932 ( 820555-901) 4.6 x 50 1.8 925975-902 825975-902 4.6 x 50 1.8 925975-932 825975-932 3/ 2.1 x 50 1.8 925700-902 825700-902 2.1 x 50 1.8 925700-932 825700-932 3/ 4.6 x 30 1.8 923975-902 823975-902 4.6 x 30 1.8 923975-932 823975-932 3/ 2.1 x 30 1.8 923700-902 823700-902 2.1 x 30 1.8 923700-932 823700-932 3/ 4.6 x 15 1.8 921975-902 821975-902 4.6 x 15 1.8 921975-932 821975-932 3/ 2.1 x 15 1.8 921700-902 821700-902 2.1 x 15 1.8 921700-932 821700-932 3/ 820555-901 820555-901 www.agilent.com/chem/store 63
ZORBAX 3.5 µm (4.6 mm) (2.1 mm) 5 µm Agilent ZORBAX 3.5 µm 5 µm 15-150 mm 1-4.6 mmlc/ms 3.5 µm 5 µm ZORBAX A ZORBAX Bonus-RP 883668-901 4.6 x 150 mm, 5 µm B ZORBAX Bonus-RP 866668-901 4.6 x 75 mm, 3.5 µm Time (min) C ZORBAX Bonus-RP 861700-901 2.1 x 50 mm, 3.5 µm : 80% 25 mm NH 4 Ac ph 5.5 20% : 1.5 ml/min 1.5 ml/min 0.3 ml/min : 40 C : 254 nm Time (min) Time (min) 1. 2. 3. 4. 3.5 µm LC/MS 64 www.agilent.com/chem/store
: ZORBAX SB-C18 866953-902 4.6 x 75 mm, 3.5 µm : A 25 mm NaH 2 PO 4 ph 3.0 B : 2.0 ml/min : 35 C : 254 nm 1. 2. 3. 4. 5. Time (min) Time (min) Time (min) 4.6 x 75 mm 3.5 µm ZORBAX 3.5 µm Eclipse (mm) Eclipse Plus C18 USP L1 Eclipse Plus C8 USP L7 Eclipse XDB-C18 USP L1 Eclipse XDB-C8 USP L7 Eclipse XDB-Phenyl USP L11 Eclipse XDB-CN USP L10 (µm) 4.6 x 150 3.5 959963-902 959963-906 963967-902 963967-906 963967-912 963967-905 4.6 x 100 3.5 959961-902 959961-906 961967-902 961967-906 961967-905 4.6 x 75 3.5 959933-902 959933-906 966967-902 966967-906 966967-912 966967-905 4.6 x 50 3.5 959943-902 959943-906 935967-902 935967-906 935967-912 4.6 x 30 3.5 959936-902 959936-906 934967-902 934967-906 4.6 x 20 3.5 932967-902 932967-906 3.0 x 150 3.5 959963-302 959963-306 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 959961-302 959961-306 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 * 2.1 x 150 3.5 959763-902 959763-902 930990-902 930990-906 * 2.1 x 100 3.5 959793-902 959793-906 961753-902 961753-906 961753-905 * 2.1 x 75 3.5 966735-902 * 2.1 x 50 3.5 959743-902 959743-906 971700-902 971700-906 * 2.1 x 30 3.5 959733-902 959733-906 974700-902 974700-906 * 2.1 x 20 3.5 972700-902 972700-906 * 1.0 x 150 3.5 963600-902 963600-906 * 1.0 x 50 3.5 965600-902 965600-906 * 1.0 x 30 3.5 961600-902 961600-906 3/ 1.0 x 17 5 5185-5921 5185-5921 4/ 4.6 x 12.5 5 820950-936 820950-937 820950-925 820950-926 820950-927 820950-935 4/ 2.1 x 12.5 5 821125-936 821125-937 821125-926 821125-926 821125-926 821125-935 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm www.agilent.com/chem/store 65
ZORBAX 3.5 µm StableBond SB-C18 SB-C8 SB-CN SB-Phenyl SB-C3 (mm) (µm) USP L1 USP L7 USP L10 USP L11 USP L56 SB-Aq 4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914 4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914 4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914 4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914 4.6 x 30 3.5 834975-902 834975-906 4.6 x 20 3.5 832975-902 832975-906 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-312 861954-309 861954-314 * 2.1 x 150 3.5 830990-902 830990-906 830990-914 * 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914 * 2.1 x 75 3.5 866735-902 * 2.1 x 50 3.5 871700-902 871700-906 871700-914 * 2.1 x 30 3.5 874700-902 874700-906 * 2.1 x 20 3.5 872700-902 872700-906 * 1.0 x 150 3.5 863600-902 863600-906 * 1.0 x 50 3.5 865600-902 865600-906 * 1.0 x 30 3.5 861600-902 861600-906 1.0 x 17 5 5185-5920 5185-5920 3/ 4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-917 820950-922 820950-933 4/ 2.1 x 12.5 5 821125-926 821125-926 821125-924 821125-926 821125-924 821125-933 820888-901 820888-901 820888-901 820888-901 820888-901 820888-901 *RR 3.5 µm 66 www.agilent.com/chem/store
ZORBAX 3.5 µm Rx Bonus-RP Extend-C18 *RR 3.5 µm Rx-C18 Rx-C8 Extend-C18 (mm) (µm) USP L1 USP L7 Bonus-RP USP L1 4.6 x 150 3.5 863967-902 863953-906 863668-901 763953-902 4.6 x 100 3.5 861967-902 861953-906 864668-901 764953-902 4.6 x 75 3.5 866967-902 866953-906 866668-901 766953-902 4.6 x 50 3.5 735953-902 3.0 x 150 3.5 863967-302 863954-306 863668-301 763954-302 3.0 x 100 3.5 861967-302 861954-306 864668-301 764953-302 2.1 x 150 3.5 830990-906 863700-901 * 2.1 x 100 3.5 861767-902 861753-906 861768-901 761753-902 * 2.1 x 50 3.5 861700-901 735700-902 * 1.0 x 150 3.5 863608-901 763600-902 * 1.0 x 50 3.5 865608-901 765600-902 * 1.0 x 30 3.5 861608-901 761600-902 3/ 1.0 x 17 5 5185-5922 5185-5923 4/ 4.6 x 12.5 5 820950-914 820950-913 820950-928 820950-930 4/ 2.1 x 12.5 5 821125-915 821125-915 821125-928 821125-930 820888-901 820888-901 820888-901 820888-901 www.agilent.com/chem/store 67
ZORBAX 4.6 mm 60% S/N 2 3 LC/MS LC ZORBAX 3.0 mm 4.6mm 50% LC/MS 0.5 ml/min (APCI) (APPI) MS 4.6 mm 2 3 HPLC 60% A B : : ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm ZORBAX SB-C18 883975-302 3.0 x 150 mm, 5 µm 20% : 80% 0.2 M Na2HPO4 + 0.1 M ph 2.6 1. 2. 3. 4. 5. 6. Time (min) Time (min) 4.6 3.0 mm 50% 68 www.agilent.com/chem/store
A B : : ZORBAX SB-C18 863953-902 4.6 x 150 mm, 3.5 µm ZORBAX SB-C18 863954-302 3.0 x 150 mm, 3.5 µm 25% : 75% 0.4% 254 nm 1. 2. 4 5,7-4.6 mm 2-3 HPLC Time (min) LC/MS : ZORBAX SB-C18 861954-302 3.0 x 100 mm, 3.5 µm A 70% + 0.4% B 30% + 0.4% 0.425 ml/min A UV 254 nm B APCI C 1. 2. 3. 4. 5. 6. LC/MS MS APCI 0.5 ml/min Time (min) www.agilent.com/chem/store 69
ZORBAX 80Å StableBond SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl (mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq 3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314 3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314 3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-309 863954-312 863954-314 3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314 ZORBAX 300Å StableBond 300SB-C18 300SB-C8 300SB-CN 300SB-C3 (mm) (µm) USP L1 USP L7 USP L10 USP L56 3.0 x 150 3.5 863974-302 863974-306 863974-309 863974-309 3.0 x 100 3.5 861973-306 ZORBAX 80Å Eclipse XDB XDB-C18 XDB-C8 XDB-Phenyl XDB-CN (mm) (µm) USP L1 USP L7 USP L11 USP L10 3.0 x 250 5 990967-302 990967-306 990967-312 990967-305 3.0 x 150 5 993967-302 993967-306 993967-312 993967-905 3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305 3.0 x 100 3.5 961967-302 961967-306 961967-312 3.0 x 75 3.5 966954-302 ZORBAX 80Å Bonus-RP Rx Rx-C18 Rx-C8 (mm) (µm) Bonus-RP USP L1 USP L7 3.0 x 250 5 880668-301 880967-302 880975-306 3.0 x 150 5 883668-301 883967-302 883975-306 3.0 x 150 3.5 863668-301 863967-302 863954-306 3.0 x 100 3.5 864668-301 861967-302 861954-306 ZORBAX 80Å Extend-C18 Extend-C18 (mm) (µm) USP L1 3.0 x 250 5 770450-302 3.0 x 150 3.5 763954-302 3.0 x 150 5 773450-302 3.0 x 100 3.5 764953-302 3.0 x 50 3.5 735954-302 70 www.agilent.com/chem/store
ZORBAX 1.0 mm LC/MS 1 mm 2.1 mm 5 50 µl/ HPLC 400 bar StableBond SB-C18 SB-C8 300SB-C18 Eclipse XDB-C18 XDB-C8 Bonus RP Extend C-18 Poroshell 1.8 µm ZORBAX 300SB-C18 : ZORBAX 300SB-C18 863630-902 1.0 x 150 mm, 3.5 µm : 60 min 2-60% B A 0.1% B 0.075% /80% : 50 µl/min : 50 C : 215 nm 2 µl rhgh 1.0 mm www.agilent.com/chem/store 71
ZORBAX 1.0 mm SB-C18 SB-C8 300SB-C18 300SB-C8 (mm) (µm) USP L1 USP L7 USP L1 USP L7 1.0 x 250 5 861630-902 * 1.0 x 150 3.5 863600-902 863600-906 863630-902 863630-906 * 1.0 x 50 3.5 865600-902 865600-906 865630-902 865630-906 * 1.0 x 30 3.5 861600-902 861600-906 SB-CN USP L10 ** 1.0 x 50 1.8 822600-902 822600-906 822600-905 3/ 1.0 x 17 5 5185-5920 5185-5920 5185-5920 5185-5920 XDB-C18 XDB-C8 Extend-C18 (mm) (µm) USP L1 USP L7 Bonus-RP USP L1 * 1.0 x 150 3.5 963600-902 963600-906 863608-901 763600-902 * 1.0 x 50 3.5 965600-902 965600-906 865608-901 765600-902 * 1.0 x 30 3.5 961600-902 961600-906 861608-901 761600-902 600 bar** 1.0 x 100 1.8 928600-902 928600-906 728600-902 3/ 1.0 x 17 5 5185-5921 5185-5921 5185-5922 5185-5923 (mm) (µm) Poroshell Poroshell 300SB-C18 300SB-C8 Poroshell 300SB-C3 Poroshell 300Extend-C18 1.0 x 75 5 661750-902 661750-906 661750-909 671750-902 3/ 1.0 x 17 5 5185-5968 5185-5968 5185-5968 *RR 3.5 µm **RRHT 1.8 µm 72 www.agilent.com/chem/store
ZORBAX LC/MS 0.5 0.3 0.1 0.075 mm 80Å 300Å / Agilent ZORBAX 0.5 0.3 mm 0.1 0.075 mm HPLC LC/MS SCX www.agilent.com/chem/store 73
A B C D E F ZORBAX 300SB-C8 5065-4460 0.3 x 150 mm, 3.5 µm ZORBAX Eclipse XDB-C18 5064-8291 0.3 x 150 mm, 5 µm ZORBAX Eclipse XDB-C18 5064-8291 0.3 x 150 mm, 5 µm ZORBAX SB-C18 5064-8255 0.3 x 150 mm, 5 µm ZORBAX 300SB-C18 5064-8267 0.3 x 150 mm, 3.5 µm ZORBAX 300Extend-C18 5065-4464 0.3 x 150 mm, 3.5 µm : + 0.05% ph = 2.2 = A + 0.045% = B 0.5%B/min 0 min = 1%B 60 min = 31%B 70 min = 50%B 75 min = 85%B 80 min = 85%B 81 min = 1%B 110 min = 1%B : 5.5 µl/min 200-500 µl/min : 30 C : 206/10 nm 450/80 nm 0.1 µl 400 300 200 100 0 1. -5 ng/100 nl 2. - -20 ng/100 nl 3. 28 ng/100 nl 4. 20 ng/100 nl 5. II 20 ng/100 nl 1 1 1 1 1 1 2 3 4 5 1 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 0 20 40 60 Time (min) ZORBAX 5 74 www.agilent.com/chem/store
: : : : ZORBAX SB-C18 5064-8255 0.3 x 150 mm, 5 µm ZORBAX SB-C18 5064-8256 0.5 x 150 mm, 5 µm ZORBAX SB-C18 863600-902 1.0 x 150 mm, 3.5 µm ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm 200 ng 0.3 mm 4.6 mm 100 0.1 mm-0.075 mm 2000 Time (min) www.agilent.com/chem/store 75
: ZORBAX SB-C18 5064-8256 0.5 x 150 mm, 5 µm : A 75% 0.4% B 25% 0.4% : 20 µl/min : 25 C : 0.1 µl 1. 2. 3. 4. 5. 6. StableBond-C18 0.5 x 150 mm 5 µm (k) 0.8% RSD (a) 0.4% RSD Time (min) 76
LC/MS ZORBAX HPLC 1100/1200 1. 1100 SCX 1100/1200 1100/1200 LC/MSD 1100/1200 2. 1100 SCX 1100/1200 1100/1200 LC/MSD www.agilent.com/chem/store 77
HPLC 2-D HPLC : : : : : MS : ZORBAX 300SB-C18 5065-9913 0.3 x 5 mm, 5 µm ZORBAX 300SB-C18 5065-9911 0.075 x 150 mm, 3.5 µm : 3% /0.1% : A =, 0.1%, B =, 0.1% : 30 µl/min : 300 nl/min : : 6 min = 3%B, 120 min = 60%B, 125 min = 80%B, 130 min = 80% B, 131 min = 3%B, 140 min = 3% B : Nano ESI, : 5L/min,.: 225 C. : : 1:35 V, : 115 V, 1:12 V, 2:3.5 V, : 80 V. ICC: on, : 4, : 150 ms; 60.000,, MS/MS : 1-8 µl Salt Step Elution: 8ml 10 mm-100 mm KCI ( 10 mm ), 125 mm, 150 mm, 200 mm, 300 mm, 500 mm, 1M. Time (min) 30 mm KCI 60 mm KCI 75 mm KCI 120 mm KCI (BSA) HPLC BSA 78 www.agilent.com/chem/store
ZORBAX Poroshell Eclipse 300SB- 300Extend Bio-SCX (mm) (µm) SB-C18 XDB-C18 C18 300SB-C8 300SB-C8 C18 II 0.8 x 50 3.5 5065-9942 0.5 x 250 5 5064-8258 5064-8286 5064-8266 0.5 x 150 5 5064-8256 5064-8287 5064-8264 0.5 x 75 5 5065-4468 0.5 x 35 5 5064-8254 5064-8296 5064-8294 * 0.5 x 35 3.5 5064-8260 5064-8298 5065-4459 0.3 x 250 5 5064-8257 5064-8269 5064-8265 0.3 x 150 5 5064-8255 5064-8291 5064-8263 0.3 x 35 5 5064-8253 5064-8297 5064-8295 0.3 x 35 3.5 5065-9912 * 0.3 x 150 3.5 5064-8261 5064-8271 5064-8267 5065-4460 5065-4464 * 0.3 x 100 3.5 5064-8259 5065-4461 5065-4465 * 0.3 x 75 3.5 5064-8270 5065-4462 5065-4466 * 0.3 x 50 3.5 5064-8300 5065-4463 5065-4467 10/ 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 *RR 3.5 µm 300SB-C18 (mm) (µm) USP L1 ** 0.1 x 150 3.5 5065-9910 ** 0.075 x 150 3.5 5065-9911 300SB-C8 USP L7 * 0.075 x 50 3.5 5065-9924 5065-9923 /5/ 0.3 x 5 5 5065-9913 5065-9914 / 5065-9915 5065-9915 *RR 3.5 µm www.agilent.com/chem/store 79
ZORBAX PrepHT ZORBAX 2000 mg 5 7 µm 5000 psi/350 bar ZORBAX PrepHT Eclipse XDB StableBond Bonus-RP Extend-C18 ZORBAX PrepHT 5 7 µm ZORBAX 100 ml/min ZORBAX PrepHT 21.2 mm 150 mm 7 µm 80 www.agilent.com/chem/store
ZORBAX PrepHT DAD Vial 1 347 Vial 2 343 Vial 3 389 MSD 1. 2. 3. 0 1 2 3 4 5 6 7 Time (min) ZORBAX SB-C18 5988-7113EN >90% 2000 mg ZORBAX PrepHT 21.2 mm 150 mm 7 µm ZORBAX SB-C18 (ml/min) (µl) 1 50 x 150 mm 100 2200 0.3 mm 2 21.2 x 150 mm 18 400 0.3 mm 877150-102 3 9.4 x 150 mm 3.5 80 0.3 mm 883975-202 4 4.6 x 150 mm 0.85 2.0 3 mm 883975-902 1100 4.6 mm 50 mm 1 2 3 4 1. 2. 3. Time (min) www.agilent.com/chem/store 81
ZORBAX PrepHT StableBond ZORBAX PrepHT 80ÅStableBond SB-C18 SB-C8 SB-CN SB-Phenyl (mm) (µm) USP L1 USP L7 SB-Aq USP L10 USP L11 PrepHT * 21.2 x 250 7 877250-102 877250-106 877250-114 877250-105 877250-112 21.2 x 150 7 877150-102 877150-106 877150-114 21.2 x 150 5 870150-902 870150-906 870150-914 21.2 x 100 5 870100-902 870100-906 870100-914 21.2 x 50 5 870050-902 870050-906 870050-914 PrepHT 17 x 7.5 2/ ** 5 820212-920 820212-915 820212-933 820212-933 820212-915 ZORBAX PrepHT 300Å StableBond 300SB-C18 300SB-C8 300SB-C3 300SB-CN (mm) (µm) USP L1 USP L7 USP L56 USP L10 PrepHT * 21.2 x 250 7 897250-102 897250-106 897250-109 897250-105 PrepHT 2/ ** 21.2 x 150 7 897150-102 897150-106 897150-109 21.2 x 150 5 895150-902 895150-906 895150-909 21.2 x 100 5 895100-902 895100-906 895100-909 21.2 x 50 5 895050-902 895050-906 895050-909 17 x 7.5 5 820212-921 820212-918 820212-924 820212-924 * PrepHT 820400-901 ** (820444-901) 1 ZORBAX PrepHT Eclipse XDB XDB-C18 XDB-C8 (mm) (µm) USP L1 USP L7 PrepHT * 21.2 x 250 7 977250-102 977250-106 PrepHT, 2/ ** 21.2 x 150 7 977150-102 977150-106 21.2 x 150 5 970150-902 970150-906 21.2 x 100 5 970100-902 970100-906 21.2 x 50 5 970050-902 970050-906 17 x 7.5 5 820212-925 820212-926 * PrepHT 820400-901 ** (820444-901) 1 82 www.agilent.com/chem/store
ZORBAX PrepHT Bonus-RP Extend-C18 (mm) (µm) Bonus-RP PrepHT * 21.2 x 250 7 878250-101 21.2 x 150 7 878150-101 Extend-C18 USP L1 21.2 x 150 5 868150-901 770150-902 21.2 x 100 5 868100-901 770100-902 21.2 x 50 5 868050-901 770050-902 PrepHT 2/ ** 17 x 7.5 5 820212-928 820212-930 * PrepHT 820400-901 ** (820444-901) 1 ZORBAX PrepHT ODS (C18) C8 (mm) (µm) USP L1 USP L7 USP L10 PrepHT * 21.2 x 250 7 877952-102 877952-106 877952-105 877952-108 877952-101 * PrepHT 820400-901 ZORBAX PrepHT Rx-SIL SIL (mm) (µm) USP L3 PrepHT * 21.2 x 250 7 877250-101 Rx-C18 USP L1 21.2 x 250 7 877967-102 PrepHT 2/ ** 17 x 7.5 5 820212-919 820212-914 * PrepHT 820400-901 ** (820444-901) 1 ZORBAX PrepHT 820444-901 PrepHT 2/ 820400-901 820385-901 www.agilent.com/chem/store 83
4.6 mm 50 mm 21.2 mm ph 10 21.2 30 50 mm 50-250 mm 5 10 µm 21.2 mm 4.6 mm C18 ph C18 100Å 400 m 2 /g 60 C* 2.0-10.0 24% 100Å 400 m 2 /g ** 1.0-8.0 N/A N/A * ph 8 60 C ph 8-10 40 C ** ph 84 www.agilent.com/chem/store
Agilent Prep C18 : Agilent Prep-C18 443905-902 4.6 x 150 mm, 5 µm : 50% 0.1% : 50% : 1 ml/min 10 µl / 0.5-50 mg/ml Prep Prep A Agilent Prep-C18 443905-902 4.6 x 150 mm, 5 µm B 443905-102 21.2 x 150 mm, 5 µm C 413910-302 30 x 150 mm, 10 µm D 413910-502 50 x 150 mm, 10 µm : : 55% :45% 0.7 ml/min 14.87 ml/min 29.77 ml/min 85.37 ml/min 240 nm : : 2 µl 42.4 µl 170 µl 488 µl 1. 2. 1mg/mL Prep-C18 www.agilent.com/chem/store 85
(, 400 bar) (mm) (µm) C18 Scalar 4.6 x 250 5 440905-902 440905-901 Scalar 4.6 x 150 5 443905-902 443905-901 Scalar 4.6 x 100 5 449905-902 449905-901 Scalar 4.6 x 50 5 446905-902 446905-901 (PrepHT) ( 820400-901)* PrepHT 21.2 x 250 10 410910-102 410910-101 PrepHT 21.2 x 150 10 413910-102 413910-101 PrepHT 21.2 x 50 10 446910-102 PrepHT 21.2 x 150 5 443905-102 443905-101 PrepHT 21.2 x 100 5 449905-102 449905-101 PrepHT 21.2 x 50 5 446905-102 446905-101 PrepHT 2/ 820400-901 820400-901 (, 400 bar) Prep 30 30 x 250 10 410910-302 410910-301 Prep 30 30 x 150 10 413910-302 413910-301 Prep 30 30 x 100 10 419910-302 419910-301 Prep 30 30 x 100 5 449905-302 449905-301 Prep 30 30 x 50 5 446905-302 446905-301 Prep 50 50 x 250 10 410910-502 410910-501 Prep 50 50 x 150 10 413910-502 413910-501 Prep 50 50 x 100 10 419910-502 419910-501 Prep 50 50 x 100 5 449905-502 449905-501 Prep 50 50 x 50 5 446905-502 446905-501 ( ) PrepHT 2/ 21.2 x 10 10 420212-902 420212-901 820444-901 820444-901 PrepHT 420420-901 420420-901 (1kg) 10 420910-902 420910-901 * PrepHT 820400-901 21.2 mm PrepHT 820444-901 30 mm 420420-901 86 www.agilent.com/chem/store
Ultron Ultron ES-OVM ES-Pepsin HPLC www.agilent.com/chem/store 87
(Prozac) : Ultron ES-OVM 702111651 4.6 x 150 mm, 5 µm : 25:75 (v/v) EtOH / 20 mm KH 2 PO 4, ph 5.5 ( NaOH) : : UV (225 nm) Courtesy of D. S. Risley and V. S. Sharp of Lilly Research Laboratories, Eli Lilly and Co. 0 5 10 15 20 25 30 Retention Time (min) ULTRON ES-OVM : Ultron ES-OVM 702111651 4.6 x 150 mm, 5 µm : 20 mm KH 2 PO 4 (ph 4.6) : 1 ml/min : 25 C : 220 nm H Cl N H C 2 H C 5 H 2 N S NH S O O O O 10 20 T ime (min) (mm) (µm) ES-OVM ES-Pepsin 10 x 150 5 722111723 4.6 x 250 10 724111653 4.6 x 150 5 702111651 822111651 4.6 x 150 5 702111651A 822111631A 2.0 x 150 5 702111610 4.0 x 10 5 712111630 832111630 88 www.agilent.com/chem/store
ZORBAX HPLC HPLC Agilent ZORBAX ZORBAX 300StableBond ZORBAX 300Extend ph 10 LC/MS ZORBAX Poroshell ZORBAX Eclipse (AAA) 14 7.5cm 24 15cm ZORBAX GF-250/450 GF-250 GF-450 4,000-900,000 ZORBAX (SAX) (SCX) Bio-SCX 89
ZORBAX ZORBAX < 2000 (300Å) MW < 50 kda MW < 1,000 kda StableBond 300SB-C8 300 SB C8 C18 C8 C3 C4 CN Poroshell 300SB-C18 Poroshell 300SB Poroshell Poroshell C18 C8 C3 StableBond 300SB-C8 4.6 x 150mm 3.5 5 µm 883995-906 863973-906 A 95% H 2 O:5% 0.1% B 5% H 2 O:95% 0.085% 60 min 0-60% B 35-40 C 1 ml/min Poroshell 300SB-C18 2.1 x 75mm 5µm 660750-902 A 95% H 2 O:5% 0.1% B 5% H 2 O:95% 0.085% 10 min 0-60% B 35-40 C 2 ml/min 90 www.agilent.com/chem/store
ZORBAX ph 0.1% 300Å 30-60 min Poroshell ph ZORBAX 300StableBond Poroshell 300SB ZORBAX 300Extend-C18 ph 6-8 M 5% 6 M + / DMSO 0.1%-1% DMSO StableBond 300SB - 80 C Poroshell 300SB - 80 C ph ph ph ph ph ph ZORBAX 300Extend-C18 ph 300Extend-C18 4.6 x 150 mm 5 µm 773995-902 A 20 mm NH 4 OH H 2 O B 20 mm NH 4 OH 80% 30 5-60% B 25-30 C (<60 C) 1 ml/min www.agilent.com/chem/store 91
ph ph MW < 50 kda 300SB-C8 4.6 x 150 mm, 3.5 µm 863973-906 MW < 1000 kda Poroshell 300SB-C18 2.1 x 75 mm, 5 µm 660750-902 MW < 25 kda 300 Extend-C18 4.6 x 150 mm, 3.5 µm 763973-902 LC/MS LC/MS LC/MS LC/MS LC/MS MS LC/MS LC/MS 2.1 mm Poroshell ph ph MW < 50 kda 300SB-C8 2.1 x 150 mm, 3.5 µm 863750-906 MW < 1000 kda Poroshell 300SB-C18 1.0 x 75 mm, 5 µm 661750-902 MW < 25 kda 300Extend-C18 2.1 x 150mm, 3.5µm 763750-902 0.5 mm 0.3 mm ph 0.1 0.075 mm LC/MS C18 92 www.agilent.com/chem/store
ZORBAX ph ph MW < 50 kda 300SB-C18 0.5 x 150 mm, 3.5 µm 5064-8268 MW < 1000 kda Poroshell 300SB-C8 0.5 x 74 mm, 5 µm 5065-4468 MW < 25 kda 300Extend-C18 0.3 x 150 mm, 3.5 µm 5065-4464 / LC/MS SCX 0.3 x 35 mm, 5 µm 5065-9912 0.075 x 150 mm, 3.5 µm 5065-9911 ph ph LC/MS 0.1-1.0% 1% ph LC/MS 10-20 mm NH 4 OH ph www.agilent.com/chem/store 93
R ZORBAX 300Å StableBond O Si R 1 ZORBAX 300StableBond OH R R 300Å 300StableBond ph ph LC/MS 300StableBond C18 C8 C3 O Si R 1 80-90 C 300StableBond 300SB-C18 300SB-C8 OH R R 0.3 0.5 mm 0.075 0.10 mm LC/MS O Si R 1 R 300StableBond * ph * ZORBAX 300SB-C18 300Å 45 m 2 /g 90 C 1.0-8.0 2.8% ZORBAX 300SB-C8 300Å 45 m 2 /g 90 C 1.0-8.0 1.5% ZORBAX 300SB-C3 300Å 45 m 2 /g 90 C 1.0-8.0 1.1% ZORBAX 300SB-CN 300Å 45 m 2 /g 90 C 1.0-8.0 1.2% *300 StableBond ph ph 6-8 <40 C 0.01-0.02 M ph ph 300Extend-C18 ph StableBond 300Å 100-500kDa 80Å 4000Da 94 www.agilent.com/chem/store
ZORBAX ZORBAX 300SB-C3 ph ZORBAX 300SB-C3 883995-909 4.6 x 150 mm, 5 µm 80 min 0-100% B A 0.5% TFA B0.5% TFA 1-50% A 50% B 1.0 ml/min 60 C % k Phenyltheptane Remaining ZORBAX 300 SB-C3 C4 Volume of Mobile Phase (ml) 300SB A ZORBAX 300SB-C18 883995-902 4.6 x 150 mm, 5 µm B ZORBAX 300SB-C8 883995-906 4.6 x 150 mm, 5 µm 1. 2. 3. C 4. C ZORBAX 300SB-C3 883995-909 4.6 x 150 mm, 5 µm 5. 6. CDR 7. D ZORBAX 300SB-CN 883995-905 4.6 x 150 mm, 5 µm 8. 9. S-100β 10. S-100α 40 min 25-70% B A 0.1% TFA B 0.09% TFA 80% /20% 1.0 ml/min 60 C 3 µg Time (min) 300SB-C18 C8 C3 CN 300SB-CN www.agilent.com/chem/store 95
HPLC UV MS : ZORBAX 300SB-C18 5064-8263 0.3 x 150 mm, 5 µm UV : : : MS : 50 min 5-55% B 55-57 min 85% B A 0.1% B 0.1% 5.5 µl/min 206 nm LC/MS:ESI LC/MSD -Vcap 4000 V 7 L/min 250 C 15 psi 50 V 300 ms 3 3 m/z 1.0 V 100 nl (4 pmol) UV at 206 nm % Relative Abundance MS Time (min) ZORBAX 300SB-C18 0.3 mm UV MS MS Time (min) LC/MS ZORBAX : ZORBAX 300SB-C18 5065-9911 0.075 x 150 mm, 3.5 µm Intens. : : : : A + 0.1% B + 0.1% 600 nl/min 25 min 2% B 52% B MS 100 fm (1 µl) 8 ZORBAX Nano HPLC 0.075 mm LC/MS Time (min) 96 www.agilent.com/chem/store
ZORBAX ZORBAX 300Å StableBond (mm) (, 400 bar) (µm) 300SB-C18 USP L1 300SB-C8 USP L7 300SB-CN USP L10 300SB-C3 USP L56 9.4 x 250 5 880995-202 880995-206 880995-205 880995-209 4.6 x 250 5 880995-902 880995-906 880995-905 880995-909 4.6 x 150 5 883995-902 883995-906 883995-905 883995-909 4.6 x 50 5 860950-902 860950-906 860950-905 860950-909 4.6 x 150 3.5 863973-902 863973-906 863973-905 863973-909 4.6 x 100 3.5 861973-902 861973-906 4.6 x 50 3.5 865973-902 865973-906 865973-905 865973-909 3.0 x 150 3.5 863974-302 863974-306 863974-309 3.0 x 100 3.5 861973-306 2.1 x 250 5 881750-902 2.1 x 150 5 883750-902 883750-906 883750-905 883750-909 * 2.1 x 150 5 863750-906 * 2.1 x 100 3.5 861775-902 861775-906 * 2.1 x 50 3.5 865750-902 865750-906 1.0 x 250 5 861630-902 * 1.0 x 150 3.5 863630-902 863630-906 * 1.0 x 50 3.5 865630-902 865630-906 3/ 1.0 x 17 5 5185-5920 5185-5920 2/ 9.4 x 15 7 820675-124 820675-124 820675-124 820675-124 4/ 4.6 x 12.5 5 820950-921 820950-918 820950-923 820950-924 4/ 2.1 x 12.5 5 821125-918 821125-918 821125-924 821125-924 9.4 x 15 840140-901 840140-901 840140-901 840140-901 820888-901 820888-901 820888-901 820888-901 (PrepHT) ( 820400-901) PrepHT 21.2 x 250 7 897250-102 897250-106 897250-105 897250-109 PrepHT 21.2 x 150 7 897150-102 897150-106 897150-109 PrepHT 21.2 x 150 5 895150-902 895150-906 895150-909 PrepHT 21.2 x 100 5 895100-902 895100-906 895100-909 PrepHT 21.2 x 50 5 895050-902 895050-906 895050-909 PrepHT 2/ 820400-901 820400-901 820400-901 820400-901 PrepHT 2/ 17 x 7.5 5 820212-921 820212-918 820212-924 820212-924 820444-901 820444-901 820444-901 820444-901 www.agilent.com/chem/store 97
ZORBAX 300Å StableBond ( ) (mm) (µm) 300SB-C18 USP L1 300SB-C8 USP L7 0.5 x 250 5 5064-8266 0.5 x 150 5 5064-8264 0.5 x 35 5 5064-8294 * 0.5 x 150 3.5 5064-8268 * 0.5 x 35 3.5 5065-4459 0.3 x 250 5 5064-8265 0.3 x 150 5 5064-8263 0.3 x 35 5 5064-8295 * 0.3 x 150 3.5 5064-8267 5065-4460 * 0.3 x 100 3.5 5064-8259 5065-4461 * 0.3 x 35 3.5 5064-8270 5065-4462 * 0.3 x 50 3.5 5064-8300 5065-4463 (PEEK ) ** 0.1 x 150 3.5 5065-9910 ** 0.075 x 150 3.5 5065-9911 * 0.075 x 50 3.5 5065-9924 5065-9923 /5/ 0.3 x 5 5 5065-9913 5065-9914 / 5065-9915 5065-9915 *RR 3.5 µm 300SB-CN USP L10 300SB-C3 USP L56 98
ZORBAX ZORBAX 300Å Extend-C18 ph ph 2-11.5 ph ph LC/MS ZORBAX 300Extend C-18 HPLC ph 2-11.5 ph ph ph ph LC/MS O O Si Si C18 C18 * ph ZORBAX 300Extend-C18 80Å 180 m 2 /g 60 C 2.0-11.5 4% * ph 8 60 C ph 8-11.5 40 C Silica Support 300Extend-C18 ph Extend-C18 C18-C18 ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm 20% 20 mm NH4OH ph 10.5 80% 1.5 ml/min 24 C 40 C k Values 4- -1- Column Volumes of Mobile Phase 10,000 www.agilent.com/chem/store 99
Extend-C18 StableBond SB-C18 ph A B C ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm ZORBAX Rx-C18 883967-902 4.6 x 150 mm, 5 µm 50% 50% + 1% TFA 60% 40% 1.5 ml/min 90 C Fraction of Toluene k Remaining A B C Column Volumes Mobile Phase 300Extend-C18 ph ph ph 2 ph 11.5 300Extend-C18 ph ph ph Extend-C18 LC/MS ZORBAX Extend-C18 773700-902 2.1 x 150 mm, 5 µm A 0.1% TFA B0.085% TFA 80% A 10 mm NH 4 OH B 10 mm NH 4 OH 80% 0.2 ml/min 15 min 15-50% B 35 C MS ESI - Vf 70V 4.5 kv, N2-35 psi, 12 L/min. 325 C 2.5 µl 50 pmol I II III B.E Boyes Separation and Analysis of Peptides at High ph Using RP-HPLC/ESI-MS, 4th WCBP, San Francisco, CA Jan 2000. TIC (200-1500 m/z) TIC (200-1500 m/z) min min ph ph ph ph Extend-C18 ph m/z m/z 100 www.agilent.com/chem/store
ZORBAX ZORBAX 300Å Extend-C18 (mm) (µm) 4.6 x 250 5 770995-902 4.6 x 150 5 773995-902 4.6 x 150 3.5 763973-902 4.6 x 100 3.5 761973-902 4.6 x 50 3.5 765973-902 * 2.1 x 150 3.5 763750-902 * 2.1 x 100 3.5 761775-902 * 2.1 x 50 3.5 765750-902 4/ 4.6 x 12.5 5 820950-932 4/ 2.1 x 12.5 5 821125-932 820888-901 *RR 3.5 µm * 0.3 x 150 3.5 5065-4464 * 0.3 x 100 3.5 5065-4465 * 0.3 x 75 3.5 5065-4466 * 0.3 x 50 3.5 5065-4467 www.agilent.com/chem/store 101
(Poroshell) Porous Shell SOLID CORE 5 µm (Poroshell) 300Å SB-C18 SB-C8 SB-C3 ph ZORBAX Poroshell 1,000 kda Poroshell 300SB ph 300Extend-C18 ph 300SB-C18 300SB-C8 300SB-C3 300Extend-C18 ZORBAX Poroshell Poroshell HPLC 500-1,000 kda Poroshell StableBond ph 2-10 Poroshell 300Extend-C18 LC/MS HPLC Poroshell 102
ZORBAX Poroshell ZORBAX Poroshell 300SB-C18 660750-902 2.1 x 75 mm, 5 µm A 0.1% TFA B 0.07% TFA 3.0 ml/min 1.0 min 5-100% B 70 C 260 bar 215 nm 1. II 2. 3. 4. 5. 6. 7. 8. ZORBAX Poroshell 300SB 90% A B ZORBAX Poroshell 300SB-C18 660750-902 2.1 x 75 mm, 5 µm ZORBAX 300SB-C18 883750-902 2.1 x 150 mm, 5 µm A 95% 5% 0.1% TFA B 5% 95% 0.07% TFA 1 ml/min 0.208 ml/min 0-100%B = 12 min 0-100%B = 120 min 70 C 20 µl (0.22 µg/1 µl) BSA 15 70 pmol ZORBAX Poroshell 1/10 www.agilent.com/chem/store 103
ZORBAX Poroshell LC/MS ZORBAX Poroshell 300SB-C18 661750-902 1.0 x 75 mm, 5 µm A + 0.1% B + 0.1% 600 µl/min 5.5 min 20-100% B 80 C MS LC/MS ESI - 6000 V 12 L/min 350 C 45 psi 140 V 600-2500 0.15 amu 0.06 min 1 µl ZORBAX Poroshell 2.1 mm 1.0 mm 0.5 mm LC/MS 1.0 mm 0.5 mm Poroshell LC/MS Poroshell 0.5 5 pmole MS Poroshell MS ZORBAX Poroshell Poroshell Poroshell Poroshell Poroshell (mm) (µm) 300SB-C18 300SB-C8 300SB-C3 300Extend-C18 2.1 x 75 5 660750-902 660750-906 660750-909 670750-902 1.0 x 75 5 661750-902 661750-906 661750-909 671750-902 0.5 x 75 5 5065-4468 4/ 2.1 x 12.5 5 821075-920 821075-918 821075-924 820888-901 820888-901 820888-901 3/ 1.0 x 17 5 5185-5968 5185-5968 5185-5968 5185-5968 104 www.agilent.com/chem/store
ZORBAX ZORBAX Eclipse (AAA) 24 OPA FMOC Agilent 1100/1200 ZORBAX Eclipse AAA 7.5 cm 14 9 15 cm 24 18 Agilent 1100/1200 HPLC OPA FMOC 5-50 pmol DAD FLD ZORBAX Eclipse-AAA 24 ZORBAX Eclipse AAA 963400-902 4.6 x 150 mm, 3.5 µm A 40 mm Na 2 HPO 4 ph 7.8 B 45:45:10 v/v 2 ml/min 40 C 24 1. Asp 2. Glu 3. Asn 4. Ser 5. Gln 6. His 7. Gly 8. Thr 9. Cit 10. Arg 11. Ala 12. Tyr 13. Cys 14. Val 15. Met 16. Nva 17. Trp 18. Phe 19. Ile 20. Leu 21. Lys 22. Hyp 23. Sar 24. Pro Time (min) 24 18 4.6 x 75 mm Eclipse AAA 9 www.agilent.com/chem/store 105
ZORBAX Eclipse (AAA) (mm) (µm) 4.6 x 150 5 993400-902 FLD 4.6 x 150 3.5 963400-902 4.6 x 75 3.5 966400-902 3.0 x 150 3.5 961400-302 4/ 4.6 x 12.5 5 820950-931 820888-901 L- L- L- L- L- L- L- L- L- L- L- L- L- L- L- L-, 10 x 1 ml * 1 nmol/µl 5061-3330 250 pmol/µl 5061-3331 100 pmol/µl 5061-3332 25 pmol/µl 5061-3333 10 pmol/µl 5061-3334 5062-2478 4 1 g * 5062-2478 1 g OPA, (OPA) 3-0.4 M 5061-3335 10 mg/ml, 6 x 1 ml FMOC, 9-2.5 mg/ml 5061-3337 1 ml, 10, 100 ml 5061-3339 DTDPA (),, 5 g 5062-2479 106 www.agilent.com/chem/store
ZORBAX ZORBAX GF-250 GF-450 ph (ph 3-8) ZORBAX GF-250 GF-450 GF-250 GF-450 4,000-900,000 GF-250/GF-450 >90% ph ph 3 ph 8 GF-250 GF-450 3 ml/min (<25%) ph ZORBAX GF-250 150Å 4 µm 4,000-400,000 ZORBAX GF-450 150Å 6 µm 10,000-900,000 140 m 2 /g 3.0-8.0 <3.0 ml/min 50 m 2 /g 3.0-8.0 <3.0 ml/min 350 bar 350 bar 107
ZORBAX GF-250 SEC ZORBAX GF-250 884973-901 9.4 x 250 mm, 4 µm 200 mm ph 7.0 2 ml/min 254 nm BioRad 1. 670,000 Da 2. g- 158,000 Da 3. 44,000 Da 4. 17,000 Da 5. B-12 1,350 Da ZORBAX GF-250 GF-450 Time (min) A BSA BSA 2 2 B. 2 1. BSA 2. BSA 3. 1. IgG 2. IgG 1K (MOPC 31C) 3. Tris Na 2 HPO 4 NaCl 1 3 1 3 3 Inj. #1 Inj. #2 1 0 5 10 Time (min) 0 1 2 3 4 5 6 7 8 9 10 Retention Time (min) ZORBAX GF-450 884973-902 9.4 x 250 mm, 6 µm ZORBAX GF-450 884973-902 9.4 x 250 mm, 6 µm PBS ph 7.4 3 ml/min 220 nm BSA BSA 200 mm ph 7.0/0.1% 2 ml/min 225 nm 10 mg 50 mm ph 7.0 108 www.agilent.com/chem/store
ZORBAX 9.4 x 250 mm ZORBAX GF-250 ZORBAX GF-250 884973-701 4.6 x 250 mm, 4 µm 130 mm NaCl/20 mm KCl/ 50 mm Na 2 HPO 4 ph 7.0 1 ml/min 210 nm 1 2 3 4 5 6 7 1. IgM 900,000 Da 2. 670,000 Da 3. ß- 200,000 Da 4. 67,000 Da 5. 45,000 Da 6. 13,700 Da 7. 65 Da 4 6 8 10 12 14 Time (min) ZORBAX GF-250 GF-250 K* K* 3.0 3.0 K* 2.5 2.0 1.5 1.0 0.5 BSA Ovalbumin Lysozyme NaN 3 K* 2.5 2.0 1.5 1.0 0.5 BSA Ovalbumin Lysozyme NaN 3 0.0 0 1000 2000 3000 Column Volumes 0.0 0 1000 2000 3000 Column Volumes www.agilent.com/chem/store 109
ZORBAX GF-250 GF-450 (mm) (µm) GF-250 150Å 9.4 x 250 4 884973-901 GF-250 150Å 4.6 x 250 4 884973-701 GF-450 150Å 9.4 x 250 6 884973-902 ( ) GF-250 Diol2/ 9.4 x 15 6 820675-111 GF-250 Diol4/ 4.6 x 12.5 6 820950-911 GF-450 Diol2/ 9.4 x 15 6 820675-111 GF-250 Diol4/ 4.6 x 12.5 6 820950-911 9.4 x 15 840140-901 820888-901 PrepHT PrepHT GF-250 150Å 21.2 x 250 6 877974-901 PrepHT GF-450 300Å 21.2 x 250 6 877974-910 PrepHT 2/ 820400-901 PrepHT GF-2502/ 17 x 7.5 6 820212-911 PrepHT GF-4502/ 17 x 7.5 6 820212-911 820444-901 110
ZORBAX ZORBAX SAX SCX ZORBAX SAX 300SCX Zorbax ph 2-7 ZORBAX Bio-SCX II Zorbax (SAX) (300SCX) 5 µm Zorbax Bio-SCX II LC/MS 3.5 µm Zorbax ph ZORBAX SAX 70Å 300 m 2 /g 2.0-7.0 350 bar ZORBAX 300SCX 300Å 50 m 2 /g 2.0-7.0 350 bar ZORBAX Bio-SCX II 300Å 90 m 2 /g 2.5-8.5 350 bar ZORBAX SAX SCX (mm) (µm) SAX 300SCX 9.4 x 250 5 880952-203 880952-204 4.6 x 250 5 880952-703 880952-704 4.6 x 150 5 883952-703 883952-704 4.6 x 50 5 846952-704 3.0 x 50 5 860700-304 2.1 x 150 5 883700-704 2.1 x 50 5 860700-704 Bio-SCX II 0.3 x 35 3.5 5065-9912 0.8 x 50 3.5 5065-9942 4/ 4.6 x 12.5 6 820950-903 820950-904 820888-901 820888-901 www.agilent.com/chem/store 111
HPLC www.agilent.com/chem/education HPLC HPLC 800-820-3278 www.agilent.com/chem/techsupport 112
ZORBAX Eclipse Plus Eclipse Plus Eclipse Plus ph Eclipse Plus 1. 2. 60/40 / Eclipse Plus C18 Ace 5 C18 SunFire C18 XBridge C18 Discovery HS C18 Luna C18 (2) Gemini C18 XTerra MS C18
www.agilent.com/chem/cn 800-820-3278 010-64397504 010-64397370 2007 2007 10 5989-5992CHCN