/ DNA DNA Clean & Concentrator DCC 讓您笑嘻嘻! DM TEL (02)2720-2215 TEL (05)2844-162 TEL (03)6684-5 8 6 TEL (06)2890-665 TEL (04)2463-3591 TEL (07)3470-143 免付費客服專線
讓您笑嘻嘻!... 1
Zymo Clean-Spin Technology spin column DNA! 0% buffer 100% elution Zymo, spin column, elution, buffer, DNA! Question Your Spin Column! 0 μl Contamination! 2-3 μl Contamination! VS. Zymo-Spin Column Supplier Q 2 讓您笑嘻嘻!
DNA ligation/cloning 實驗常見問題 可能原因 Inhibitor 可能的來源 PCR efficiency 差 總是做不出來 一直 p 不出來 qpcr DNA 定序 失敗 失敗 這可能是因為 DNA 不純 含有過多的 inhibitors 像是鹽類 (例 guanidinium KCl NaCl) ionic detergents (例 sodium deoxycholate sarkosyl SDS) alcohols (ethanol isopropanol phenol) 這 些 PCR inhibitors 通 常 會 透 過 和 DNA 作用或抑制 DNA polymerase 影響放大反應 另外 不純的純化過程 可能 殘留 polyphenols (例 melanin 或 humic acid) 會與 Mg2+ 螯合 影響 PCR 存在於原本的樣本 (例 組織 血液 土壤 植物等等) DNA 純化過程仍無法完全移除 DNA 純化過程所造成的 解決方法 使用 Zymo DCC 系列產品 將 inhibitor 或 雜質完全移除 純化超高純度 DNA 方便 進行後續實驗 讓您笑嘻嘻 3
Oligo Clean-up Oligo Clean & Concentrator TM : 2 labeling (radioactive biotin DIG ) DNA oligonucleotides ( 16 nt), enzymes nucleotides dyes salts :DNA 6 µl DNA PCR probe hybridization sequencing ligation Oligo Clean & Concentrator TM recovery, > 90% DNA oligonucleotides, short oligo (<6 mer) nucleotide A Oligonucleotide Recovery B Nucleotide Retention The Oligo Clean & Concentrator TM facilitates >90% recovery of ssdna oligonucleotides (A) and efficient short oligo and nucleotide removal (B). RNA! Oligo Clean & Concentrator TM D4060 (50 rxns) D4061 (200 rxns) DNA size 16 nt, up to 23 kb Binding capacity 5 µg Elution volume 6 µl Processing time 2 min 4 讓您笑嘻嘻!
DNA purification from PCR & Enzymatic Reactions DNA Clean & Concentrator TM DCC TM : 2 PCR DNA :DNA 6 µl DNA ligation sequencing labeling PCR microarray transformation restriction enzyme digestion DCC, DNA DCC DNA DNA amplicons can be efficiently purified and concentrated using the DCC TM. High efficiency DNA recovery using the DCC TM -5. Equivalent amounts of DNA were purified using the DCC TM -5 or MinElute Kit and analyzed by electrophoresis in a 0.8% (w/v) agarose/ TAE/EtBr gel 讚! Illumina Nextera TM Sample Prep Infinium HD FFPE Restore, DNA Clean & Concentrator TM NuGen WT-Ovation TM Pico RNA Amplification System, DNA Clean & Concentrator TM -25 amplified cdna DNA Clean & DNA Clean & DNA Clean & DNA Clean & Concentrator TM -5 Concentrator TM -25 Concentrator TM -100 Concentrator TM -500 D4013 (50 rxns) D4033 (50 rxns) D4029 (25 rxns) D4031 (10 rxns) D4014 (200 rxns) D4034 (200 rxns) D4030 (50 rxns) D4032 (20 rxns) DNA size 50 bp-23 kb 50 bp-23 kb 50 bp-23 kb 50 bp-23 kb Binding capacity 5 µg 25 µg 100 µg 500 µg Elution volume 6 µl 25 µl 150 µl 2 ml Processing time 2 min 2 min 15 min 25 min 讓您笑嘻嘻! 5
Genomic and large DNA Clean-up Genomic DNA Clean & Concentrator TM : 5 ( :Proteinase K digestion) DNA ( : genomic, mitochondrial, BAC/PAC, viral, phage, WGA DNA) :DNA 10 µl DNA PCR restriction endonuclease digestion Sanger Next-Gen sequencing Genomic DNA Clean & Concentrator TM DNA Lambda phage DNA.λDNA (48.5 kb) is effectively recovered from various concentrations of starting material using the Genomic DCC TM. High molecular weight DNA is efficiently purified using the Genomic DCC TM. Porcine gdna (~35-50 kb), T4 phage DNA (170 kb), and lambda phage DNA (48.5 kb) were purified using the Genomic DCC TM. Samples are resolved in a 0.8% TAE/agarose/ EtBr gel. The size marker M is a 1 kb ladder (Zymo Research). 讚! Genomic DNA Clean & Concentrator TM column, column matrix, DNA ( 10 µg) Illumina Nextera TM DNA Sample Prep kit, Genomic DNA Clean & Concentrator TM DNA, DCC selection guide Genomic DNA Clean & Concentrator TM D4010 (25 rxns) D4011 (100 rxns) DNA size 50 bp-200 kb Binding capacity 10 µg Elution volume 10 µl Processing time 5 min 6 讓您笑嘻嘻!
Boost DNA Recoveries from Agarose Gels to > 80% Zymoclean TM Gel DNA Recovery Kit DNA :50 bp - 10 kb, 70-90%;11 kb - 23 kb, 50-70% : 15 agarose gel DNA :DNA 6 µl DNA DNA ligation sequencing labeling PCR microarrays transformation restriction digestion DNA (> 20 kb) DNA DNA fragments recovered from an agarose gel using the Zymoclean TM Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments. DNA Recovery of large DNA fragments. The Zymoclean TM Large Fragment DNA Recovery Kit was used to recover λ DNA digested with HindIII and separated by agarose gel electrophoresis. Lane C:λ-HindIII digest; lanes 1 & 3: recovered 23 kb λ-hindiii fragments; lanes 2 & 4: recovered 9 kb λ-hindiii fragments. Lane λ: intact λ phage DNA; lanes 5, 6: intact λ ~48 kb bands. Zymoclean TM Gel DNA Recovery Kit Zymoclean TM Large Fragment DNA Recovery Kit D4007 (50 rxns) D4045 (25 rxns) D4008 (200 rxns) D4046 (100 rxns) DNA size 50 bp-23 kb 50 bp-200 kb Binding capacity 5 µg 10 µg Elution volume 6 µl 10 µl Processing time 15 min 15 min 讓您笑嘻嘻! 7
ChIP DNA Purification ChIP DNA Clean & Concentrator TM : 2 ChIP assay DNA, cell lysates proteinase K digestion PCR :DNA 6 µl DNA PCR arrays DNA quantification Southern blot sequencing cell lysates DNA Agarose gel electrophoresis of DNA isolated from cell lysates. High quality DNA can be efficiently recovered from Saccharomyces cerevisiae cell lysates using the ChIP DNA Clean and Concentrator TM. Duplicate purifications were performed with 5, 15 and 30 µl cell lysate and an equal volume of eluted DNA was loaded into each lane. The size marker M1 and M2 are 100 bp and 1 kb ladders, respectively (Zymo Research). ChIP DNA Clean & Concentrator TM D5205 DNA size 50 bp-23 kb Binding capacity 5 µg Elution volume 6 µl Processing time 2 min 8 讓您笑嘻嘻!
DNA Too Dirty for PCR? Clean it with OneStep! OneStep TM PCR Inhibitor Removal Kit inhibitors, polyphenolics humic/fulvic acids tannins melanin DNA, PCR sequencing RT 5 inhibitor-free DNA DNA ( RNA ) humic acid, PCR DNA is efficiently amplified by PCR following humic acid removal with the OneStep TM PCR Inhibitor Removal Kit. The figure shows amplification of a 200 bp product from DNA containing humic acid that was treated with the kit. The ladder is a 100 bp DNA marker (Zymo Research). RNA,, PCR PCR amplification of an eukaryotic transcript (post-rt): Total RNA isolated from sludge with or without inclusion of the Zymo-Spin TM IV-HRC Spin Filter. M is a 1 kb DNA Marker (Zymo Research). RNA! OneStep TM PCR Inhibitor Removal Kit D6030 Elution volume 50-200 µl Processing time 5 min 讓您笑嘻嘻! 9
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DCC?! DCC! 讓您笑嘻嘻! 11
endotoxin-free Plasmid DNA ZR Plasmid Miniprep TM - Classic DNA:plasmid DNA endotoxin-free, transformation cloning transfection in vitro transcription : buffer, 3 buffer 3 Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep - Classic, performed in triplicate. ZR Plasmid Miniprep TM - Classic Kit D4015 (100 rxns) D4016 (400 rxns) D4054 (800 rxns) Culture input 0.5-5 ml Plasmid DNA size Up to 25 kb Binding capacity 25 µg Elution volume 30 µl Processing time 15 min 12 讓您笑嘻嘻!
mt DM, 2015 3, TEL (02)2720-2215 TEL (05)2844-162 TEL (03)6684-5 8 6 TEL (06)2890-665 TEL (04)2463-3591 TEL (07)3470-143 免付費客服專線