II ABSTRACT Severe acute respiratory syndrome (SARS) was the first severe infectious disease in the 21 century,which was caused by a new coronavirus,n
|
|
- 矮赎 索
- 5 years ago
- Views:
Transcription
1 I SARS 21 SARS-CoV GeneBank SARS-CoV Tor2 PCR N PN422 N 40% SARS N 95% N C N 38 15%~30% SARS N N 39 Western N 30 60aa 160~170aa N Bal b/c 8 39 Western Blot N N IgG M 1 SARS-CoV N
2 II ABSTRACT Severe acute respiratory syndrome (SARS) was the first severe infectious disease in the 21 century,which was caused by a new coronavirus,named by WHO as SARS coronavirus (SARS-CoV). From the Genebank we got the genic sequences of Nucleocapsid (N) protein of SARS-CoV. Then according to the sequence of strain Tor2 we synthesized our genic sequence of N protein by the method of PCR. The N gene was cloned into a prokaryotic expression plasmid, named as PN422. The recombinant plasmid was expressed in E. coli. The expressed recombinant N protein account for more than 40% quantity of bacterial total protein, and most existed as soluble protein. The Western blot using a SARS patient acute phase serum showed that both soluble protein and insoluble protein had obvious biological activity. More than 95% purity can be obtained after purification, and the reactivity with serum remained similar as pre-purification. We designed a series of Nucleocapsid protein with its N or/and C terminal truncated, constructed the recombinant plasmid and expressed them in E.coli. The yields level of these thirty-eight truncated recombinant proteins were from 15% to 30%. The proteins were purified by metal chelate affinity chromatography or by electro-elution. Western Blot was used to detect the reactivity of these proteins against the convalescent sera from SARS patients. The results showed that the reactivity of the full length N protein was the best, which suggests that the integrality of N protein be important for the exposure of the prominent epitopes. After analysis of the Western Blot mode of the whole 39 recombinant proteins, we presume that the nitrogen terminal (30~60aa) and the middle region (160~170aa) might exist two important linear epitpes. The Bal b/c mice were immunized with the N proteins. Using the conventional technique of monoclonal antibody we filtrate out eight hybridomas. By the method of Western Blot and competitive inhibition assay, we categorized the eight monoclonal antibodies into two classes. The first class consisted of six monoclonal antibodies,and they recognized the immunity dominant epitope of N protein,which located between the 30th and the 184th amino acid. The second class consisted of two monoclonal antibodies, the epitope they recognized located between 301st and 360th amino acid. After identifed the subclass the titer and the affinity of the representative antibodies of the 2 class relatively, we found that they both belong to the IgG1 subclass, their ascites titers were 10 6 and their affinity were 10 9 M -1. Key word: SARS-CoV Nucleocapsid protein Monoclonal antibody
3 1. SARS SARS 3. SARS SARS SARS SARS-CoV SARS-CoV SARS-CoV SARS-CoV RNA SARS-CoV SARS-CoV SARS-CoV N SARS-CoV N 15
4 4 3.2 SARS-CoV N SARS-CoV N SARS-CoV N SARS-CoV N SARS-CoV N N N 43. SARS-CoV N PN N N PCR SARS-CoV N N N SARS 59. SARS-CoV N 60
5 N D4 4F D4 4F D4 4F D4 4F SARS-CoV N N N
6 6 Contents Abstract Chapter1 Preface 1 The epidemiology of SARS 1 1. The infectious source 2 2. Routes of transmission 2 3. Clinical symptom and death rate 2 Isolation of the pathogen of SARS 3 Special diagnosis of SARS 4 1. Serologic diagnosis 4 2. Nucleic acid diagnosis 5 3. Isolation of the virus from the culture cells 6 4. Electron microscopy(em) 6 SARS virus 7 1. Introduction to coronavirus 7 2. The biological properties of SARS-CoV The genome sequence of SARS-CoV The mutation of the genome of SARS-CoV The Proteome of SARS-CoV Replicase polyprotein of SARS-CoV The structure protein of SARS-CoV The resistibility and stability of SARS The nucleocapsid protein of SARS-CoV The bio-informational analysis of SARS N protein 15
7 3.2 The properties and functions of SARS-CoV N proteins The immunogenic properties of SARS-CoV N proteins Research of the epitopes of SARS-CoV N proteins The monoclonal antibody research of SARS-CoV N proteins 17 The purpose and meaning of this research 17 Chapter2 Materials and Methods Materials Methods 26 Chapter3 Results and Analysis 41 Acquirement of the SARS-CoV N protein gene Synthesis of the N protein gene Analysis of the properties of N protein 43 Construction of the expression vector of SARS-CoV N protein and its expression and purification Construction of the expression plasmids Expression purification and activity identification 49 Acquirement of a series of truncated N proteins Design of primers Amplification of gene fragments by PCR Construction of the recombinant plasmids containing the fragments Identification of the recombinant plasmids by the small quantity expression Large scale expression of the N recombinant proteins 55
8 8 6. Purification of the N recombinant proteins WB assay of the recombinant proteins against the convalescence sera from SARS patients 59 Generation of N protein McAb and the initial research of its properties Establishment of the hybridoma strains and induction of the ascites The WB reactivity of the McAbs against the N recombinant proteins The competitive inhibition assay of the McAbs Classification of the 8 McAbs obtained The titer of McAb 4D4 and 4F The purification of McAb 4D4 and 4F The subclass identification of McAb 4D4 and 4F The affinity determination of McAb 4D4 and 4F4 69 Chapter4 Discussion Acquirement of SARS-CoV N proteins Epitope study of N proteins Research of McAbs to N protein 73 Brief summary 76 References 77 Acknowledgements 82 appendix 83
9 Severe Acute Respiratory Sysndrome SARS [1, 2]. SARS SARS [3] 1 SARS Fig 1 The globle distribution of SARS (
10 2 1. SARS SARS 8 [4, 5] SARS SARS 10 [6] SARS 2. SARS SARS [7] [8, 9] 3. SARS [10] 10%~20% ARDS [11] SARS [6] WHO SARS 0 50 SARS
11 WHO SARS [12, 13] 50 SARS [14] 40 RNA 200 CDC SARS SARS WHO Erasmus Albert Osterhaus [15] KM [16] SARS SARS-CoV
12 4 [17]. SARS SARS Roche SARS PCR SARS CDC SARS SARS PCR SARS - RNA 1. (enzyme linked immunosorbent assay ELISA) ELISA IgM IgG IgM IgG IgM SARS WHO SARS ( ) 21 d ( Immunofluorescence assay IFA) SARS SARS 10 d SARS
13 5 SARS SARS SARS SARS - - SARS SARS SARS 2. PCR SARS - ( reverse t ranscriptase polymerase chain reaction RT-PCR) SARS RNA 10 d SARS 20 90
14 6 SARS SARS (40 80nt) (5 30nt) 5 3 SARS SARS SARS SARS ( 9 h) 3. (VERO E6) SARS 24 h SARS 3 P3 SARS SARS P3 4. VERO E6 SARS SARS
15 7 SARS. SARS SARS [18, 19] SARS (SARS-CoV) (Order Nidovirales) (Family Coronaviridae) (Genus Coronavirus) RNA nm 2 [20] Coronavirus 2. Figure 2 The electron micrograph of SARS-CoV Department of Microbiology, University of Leicester
16 8 S (Spike Protein) M (Membrane Protein) E (Envelope Protein) HE (Haemagglutinin-esterase) S - [21] M M M [22] E M E [23] 3. Figure 3 The particle structure of SARS-CoV ( N Engl J Med,2003,348:1948~1951)
17 Degree papers are in the Xiamen University Electronic Theses and Dissertations Database. Full texts are available in the following ways: 1. If your library is a CALIS member libraries, please log on and submit requests online, or consult the interlibrary loan department in your library. 2. For users of non-calis member libraries, please mail to etd@xmu.edu.cn for delivery details.
5 5 7 5 4 4 6 9 8 40
5 5 7 5 4 4 6 9 8 40 4 5 0 959 68 959 55 959 4 957 4 5 998 4 9 959 98 8 980 8 84 4 987 4 5 6 7 8 9 986 998 989 4 977 8 5 988 6 985 8 7 98 990 5 8 998 4 9 987 4 5 4 96 9 959 8 959 4 986 4 5 5 8 96 4 5
More informationDegree papers are in the Xiamen University Electronic Theses and Dissertations Database. Full texts are available in the following ways: 1. If your library is a CALIS member libraries, please log on http://etd.calis.edu.cn/
More information2
1999 4 2 3 4 5 6 7 8 9 10 11 1 2 1999 1 5 1998 7 2 3 97 7 37 12 4 5 1998 8 13 14 5 1997 10 15 6 27 36 16 Degree papers are in the Xiamen University Electronic Theses and Dissertations Database. Full texts
More information1 2.1 2.2 3.2 3.3 2 3 2.1 2.2 4 5 6 7 8 9 10 11 12 13 14 15 16 Degree papers are in the Xiamen University Electronic Theses and Dissertations Database. Full texts are available in the following ways: 1.
More information1 1 2 1 2 1 2 3 1 2 3 2 3 1 355 110 403 62 1979 1998 17 1979 2 1996 70 10 4000 200 332 17 80 3000 1.7 1% 300 4 1998 60% 3.4% 0.9 3.6 40.8 37.4 17.3 0.3 4.4 42.6 36.0 16.7 7.1 3.6 67.9 10.7 10.7 3.2 65.6
More information-2-
-1-20 -2- -3- -4- -5- -6- -7- -8- -9- -10- -11- -12- -13- -14- -15- -16- Degree papers are in the Xiamen University Electronic Theses and Dissertations Database. Full texts are available in the following
More information10384 9707005 UDC 1 1 5 5 5 6 7 10 10 10 12 13 16 16 17 18 19 21 21 23 24 27 27 27 28 28 29 29 31 32 1789 1846 1857 1859 46 173 34 35 28 442 5 319 88 87 46 211 2 82 46 219 1862 1945 Degree papers
More information2
10384 -------- 9601008 UDC---------------------- 1999 1999 1999 ------------------------ ------------------------ 1 2 .4...4 7 14.14..18..22..22..24 3 4.27.32.39 1 2 3 4 5 6 7 8 9 10 11 5 12 6 1 2 3 4
More information10384 9715101 UDC MBA 2000 5 2000 6 2000 2000 1995 10 26 1995 1995 10 1999 7 1 1986 SEC, Securities Exchange Committee 20 7 1996 1998 1998 1 2 3 1999 4 1 1 3000 2 3 1997 5 Degree papers are in
More information10384 1 X9915063 UDC The Brand Marketing of Real Estate MBA 2002 8 2002 9 2002 2002 8 1 1 2...1...4...4...4...4...5...6...6...6...6...7...9...9...9...10...10...11...11...12...12...14...14 1 3...16...16...17...18...18...18...19...20...20...22...23...23...24...25...26...27...28...28...30...32...32...32...33...34...34
More informationSupply Chain SCM IBM DRP
10384 9915114 UDC 2001 9 Supply Chain SCM IBM 21 1996 DRP Keywords Xiamen Overseas Chinese Electronic Co., Ltd Chinese Color TV Enterprise Transaction Cost the Management of Production, Marketing and Inventory
More information2000.11 2000.11 2000 11 Abstract: Background: The development of autoantibodies in the sera of patients with cancers has been described, but the mechanism is still not clear. Objective: The aim of this
More information50 2
Frost & Sullivan 2006 65% 1 50 2 IDCITUMII1990 1999 10%, 49.2% 1-1 1-1 3 4 2005 GSM 3 2006 3G 2008 GSM GPRSCDMA 5 6 7 8 Frost & Sullivan 2006 65% 9 Email Ovum 2005 19.7% 164 10 11 12 13 14 Jupiter Media
More information() MONORCHIIDAE SP
10384 B953404 UDC Monorchiidae sp ...2...3...5... 5... 5...8... 8....8....8 ()....12... 13....13....24...36.... 38....38....40...44 MONORCHIIDAE SP.... 47....47...49....50...51...53...57...58...61 1 2
More informationIPOs ... 1... 3...3...6... 8... 10... 12... 14...15... 15... 15... 16...16... 20...20...22... 24...24... 24... 25... 27 BP...28 RBF...31 MATLAB... 34 MATLAB...34 MATLAB... 34... 34... 35 MATLAB...36...
More information2
10384 K9607315 U D C 1999 1999 1999 1999 3 2 30 1957 1953 1953 1958 1978 1955 1956 1979 60 1980 BG(! BHDFG3 FK3 K6 K2 K12 K3 K7F BHDG3 FK3 K20ZQ K3 K7F BHDG21 CD#4 CD#4 CD#12 BHDG5 FK33ZQF SQ*2 JY(Z
More information1
10384 9832009 UDC 2001 6 1 2...3....3....3...5...5...6...9...9...12...15...15...18...38...42...43...46 3.. 7 2000 4 10 2000 8 www.wisecast.com 2000 12 22 4 5 1993 7 1 25% 1996 20 1997 67 1998 210 1999
More information% 6.7% % % / 1
1 1995 1995 50% 70 75% 1996 1996 9 30 5000 2000 1996 1999 1998 11 9 1999 5 9 5 1998 10 1999 30 200 2 1950 38 1999 2960 1950 40 1999 3585 1950 0.73 1999 28.3 20 12.1% 6.7% 1 1 2 1950 1978 1950 38 1978 466
More information.1..2....2 2. 2.. 3..5 7.7.8..11 11..12...14 19...19 20 20 26.29 36 40...41 90 98 4 21 Network selling (Multilevel Marketing),, 1950 1 1 2 3 LeeMythnger WillianCasselberry 1959 60 60 200 60 1972 40 80%
More information1990-1997 1980-1997 Abstract The relationship between human resource development and economy increase has been an important research item with the coming of knowledge economy. In this paper, ahead item
More information10384 X9908009 UDC Study On Causation Of Civil Tort Liability 2004 4 2004 2004 2004 4 [M] 1991476 [M] 1996635 ABSTRACT ABSTRACT Theory of causation, which is the most complicated and thorny of tort law,
More informationA Study on Grading and Sequencing of Senses of Grade-A Polysemous Adjectives in A Syllabus of Graded Vocabulary for Chinese Proficiency 2002 I II Abstract ublished in 1992, A Syllabus of Graded Vocabulary
More informationXML SOAP DOM B2B B/S B2B B2B XML SOAP
10384 9831010 U D C B2B 2 0 0 1 4 2 0 0 1 5 2 0 0 1 2001 4 XML SOAP DOM B2B B/S B2B B2B XML SOAP ABSTRACT Based on the research of Supply Chain Management theory and E-Commerce theory, especially in Business
More information1998 5
10384 9511001 1998 5 1998 6 1998 1998 5 AICPA Internet/Intranet Internet/Intranet 1 2 2 2 4 6 10 10 10 12 13 13 14 18 18 18 19 19 20 Internet/Intranet 20 Internet/Intranet 23 21 25 28 28 28 30 32 32 32
More information1
10384 9915009 UCD RESEARCH STAFF CAREER MANAGEMENT IN STATE-OWNED COMMERCIAL BANKS 1 Abstract The reasonable disposition of human resources is the guarantee of the long prosperity of enterprise. To carry
More informationTHE APPLICATION OF ISOTOPE RATIO ANALYSIS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETER A Dissertation Presented By Chaoyong YANG Supervisor: Prof.D
10384 070302 9825042 UDC 2001.6. 2001.7. 20016 THE APPLICATION OF ISOTOPE RATIO ANALYSIS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETER A Dissertation Presented By Chaoyong YANG Supervisor: Prof.Dr. Xiaoru
More information10384 X0015101 UDC The Preliminary Survey of the Development Patterns of Security Analysts in China (MBA) 2004 2 2004 3 2004 3 2 0 0 4 2 14 Abstract Abstract The security analysts are respectable oversea,
More informationWTO
10384 X0115018 UDC MBA 2004 5 14 2004 6 1 WTO 2004 2006 7 2 Abstract According to the promise after our country enter into WTO, our country will open the readymade oil retail market in the end of 2004
More information:
A Study of Huangtao : I Abstract Abstract This text focuses on the special contribution of Huangtao in the history of literature and culture of Fukien, by analyzing the features of Huangtao s thought,
More informationWTO
10384 200015128 UDC Exploration on Design of CIB s Human Resources System in the New Stage (MBA) 2004 2004 2 3 2004 3 2 0 0 4 2 WTO Abstract Abstract With the rapid development of the high and new technique
More information10384 200115009 UDC Management Buy-outs MBO MBO MBO 2002 MBO MBO MBO MBO 000527 MBO MBO MBO MBO MBO MBO MBO MBO MBO MBO MBO Q MBO MBO MBO Abstract Its related empirical study demonstrates a remarkable
More informationAbstract After over ten years development, Chinese securities market has experienced from nothing to something, from small to large and the course of
2003 MBA 600795 SWOT Abstract After over ten years development, Chinese securities market has experienced from nothing to something, from small to large and the course of being standardized. To all securities
More informationAbstract The adult higher education is a vital part of the higher education. As an essential tache of selecting qualified students, the adult college entrance examination plays an important role in securing
More informationRoderick M.Chisholm on Justification I Synopsis Synopsis Since the problem of Gettier, the problem of justification has become the core of contemporary western epistemology. The author tries to clarify
More information10384 19020101152519 UDC Rayleigh Quasi-Rayleigh Method for computing eigenvalues of symmetric tensors 2 0 1 3 2 0 1 3 2 0 1 3 2013 , 1. 2. [4], [27].,. [6] E- ; [7], Z-. [15]. Ramara G. kolda [1, 2],
More information:
Thesis on Court Banquet and Assemblage Poetry in Early Tang : I Abstract Abstract In the background of three different stages early Tang: Taizong GaozongWuhou and Zhongzong,this thesis mainly analyzes
More informationUDC The Policy Risk and Prevention in Chinese Securities Market
10384 200106013 UDC The Policy Risk and Prevention in Chinese Securities Market 2004 5 2004 2004 2004 5 : Abstract Many scholars have discussed the question about the influence of the policy on Chinese
More informationABSTRACT ABSTRACT Based on analyzing public corporation in foreign countries, this paper studies basic theories of public legal establishment, with our country s reality in the social transferring period
More information( ) ( ) ATPase 1 ATPase ATPase 30 20 30 2 ATPase -. 3 MDA O 2 30 20 30 MDA 40 SOD MDA 0-10 0-30 10 3 0 SOD 0-10 10 4 NaCl 0-10 0-20 MDA Comparative Studies on Plasma Membrane Function of Salt-secreting
More informationAbstract There arouses a fever pursuing the position of being a civil servant in China recently and the phenomenon of thousands of people running to a
Abstract There arouses a fever pursuing the position of being a civil servant in China recently and the phenomenon of thousands of people running to attend the entrance examination of civil servant is
More information10384 X0115071 UDC The Research For The Actuality And Development Stratagem Of The China Securities Investment Fund (MBA) 2003 11 2003 12 2003 12 2 0 0 3 11 100 1991, WTO Abstract Abstract The Securities
More information厦 门 大 学 学 位 论 文 原 创 性 声 明 本 人 呈 交 的 学 位 论 文 是 本 人 在 导 师 指 导 下, 独 立 完 成 的 研 究 成 果 本 人 在 论 文 写 作 中 参 考 其 他 个 人 或 集 体 已 经 发 表 的 研 究 成 果, 均 在 文 中 以 适 当 方
学 校 编 码 :10384 分 类 号 密 级 学 号 : UDC 硕 士 学 位 论 文 浙 江 省 人 事 考 试 突 发 事 件 应 对 策 略 探 析 An Exploration of Zhejiang Province Personnel Examination Emergency Strategy 姜 海 峰 指 导 教 师 姓 名 : 王 玉 琼 教 授 专 业 名 称 : 公 共
More informationAbstract Since 1980 s, the Coca-Cola came into China and developed rapidly. From 1985 to now, the numbers of bottlers has increased from 3 to 23, and
Abstract Since 1980 s, the Coca-Cola came into China and developed rapidly. From 1985 to now, the numbers of bottlers has increased from 3 to 23, and increases ulteriorly. When the Coca-Cola company came
More information学 校 编 码 :10384 分 类 号 密 级 学 号 :X2007155130 UDC 厦 门 怡 福 养 生 健 康 管 理 有 限 公 司 创 业 计 划 王 韬 指 导 教 师 姓 名 : 郭 霖 教 授 厦 门 大 学 硕 士 学 位 论 文 厦 门 怡 福 养 生 健 康 管 理 有 限 公 司 创 业 计 划 A Business Plan for Xiamen Eve Health
More informationWTO OEM
10384 200115142 UDC A Study on the Developing Strategy of Xiamen Evere Sports Goods Co., Ltd. A Case Study based on the Theory of Value Chain (MBA) 2005 5 2005 6 2005 5 2005 5 WTO OEM Abstract Abstract
More information10384 200008009 UDC 2003 11 2003 2003 2003 11 1 3 3 3 4 4 4 4 5 6 6 7 7 10 10 10 11 11 17 23 23 24 25 28 29 31 33 33 36 39 41 42 42 42 42 43 44 45 45 45 46 47 47 47 50 52 54 57 58 59 60 appeal on public
More informationX UDC A Post-Evaluation Research on SINOPEC Refinery Reconstruction and Expanding Project MBA 厦门大学博硕士论文摘要库
2003 2 10384 X9915078 UDC A Post-Evaluation Research on SINOPEC Refinery Reconstruction and Expanding Project MBA 2003 2 2003 3 2003 200 / Abstract Post-evaluation is a comprehensive evaluation on an implemented
More information10384 199928010 UDC 2002 4 2002 6 2002 2002 4 DICOM DICOM 1. 2. 3. Canny 4. 5. DICOM DICOM DICOM DICOM I Abstract Eyes are very important to our lives. Biologic parameters of anterior segment are criterions
More information10384 X0115019 UDC (MBA) 2004 5 2004 6 2004 XTC An Research on Internationalization Strategy of XTC , XTC XTC XTC APT XTC XTC XTC XTC XTC XTC : Abstract Abstract Although it s well known that China s
More informationABSTRACT ABSTRACT As we know the Sinology has a long history. As earily as 19 th century some works have already been done in this field. And among this the studies of lineages and folk beliefs in Southeast
More information10384 X2009230010 UDC The Design and Implementation of Small and Medium-sized Courier Company Logistics Vehicle Scheduling System 2012 06 Abstract With the arrival of the information age, tremendous
More informationI
The Discussion on Manchu-City of Foochow in Qing Dynasty I Abstract II Abstract This thesis aims to, from the research on the Eight-Banner garrison in Foochow, explore the living situation of the banner-people
More information10384 27720071152270 UDC SHIBOR - Research on Dynamics of Short-term Shibor via Parametric and Nonparametric Models 2 0 1 0 0 5 2 0 1 0 0 5 2 0 1 0 0 5 2010 , 1. 2. Shibor 2006 10 8 2007 1 4 Shibor
More information10384 9917001 UDC 2002 2002 2002 2002 5 Research on Martin Trow s Ideological Theory of Higher Education Massification ABSTRACT The present paper is divided into five chapters. The first chapter is an
More informationAbstract Today, the structures of domestic bus industry have been changed greatly. Many manufacturers enter into the field because of its lower thresh
SWOT 5 Abstract Today, the structures of domestic bus industry have been changed greatly. Many manufacturers enter into the field because of its lower threshold. All of these lead to aggravate drastically
More information厦 门 大 学 学 位 论 文 原 创 性 声 明 本 人 呈 交 的 学 位 论 文 是 本 人 在 导 师 指 导 下, 独 立 完 成 的 研 究 成 果 本 人 在 论 文 写 作 中 参 考 其 他 个 人 或 集 体 已 经 发 表 的 研 究 成 果, 均 在 文 中 以 适 当 方
学 校 编 码 :10384 学 号 :17820121151192 硕 士 学 位 论 文 携 程 与 艺 龙 竞 争 战 略 的 财 务 比 较 分 析 Financial Analysis on Different Competitive Strategies of Ctrip and Elong 郑 亮 指 导 教 师 : 黄 海 玉 专 业 名 称 : 旅 游 管 理 硕 士 答 辩 日
More informationµ γ α γ α µ α µ γ Ig/Ig-TCIC 1 immune immunity immunis 10 998~1022 19 Edward Jenner Jenner Louis Pasteur 1880 Elie I.I. Metchnikoff (1883), P.P.Emile Roux A.E.J.Yersin (1888) Emil A. Von Behring Pfeiffer
More information10384 200326107 UDC Cloning and Expression of Genes from Venom Gland of Bungarus multicinctus 2006 7 1 2 V 1 1 2 C 4 α- P22-A31 0 11 1 11 2 11 13 α- 13 2 α- 14 3 α- 15 4 α- 15 17 α cdna 21 1 21 2 23 24
More informationUDC 厦门大学博硕士论文摘要库
10384 9924012 UDC 2002 5 2002 2002 2002 5 1 Study on High Speed Switch System and Their ASIC Frontend Design Thesis for MS By Shuicheng Cai Supervisor: Prof. Donghui Guo Department of Physics Xiamen Unviersity
More informationX MGC X 22 X 23 X MGC X BALB/c 26 X MGC X MGC X MGC X..
1 0 3 8 4 9726023 UDC X MGC80-3 2000 7 2000 7 2000 7 2000 6 22.3.4...11..11 12 13 15 15 20 20 X MGC80-3. 22 X 22 X 23 X MGC80-3.23 X BALB/c 26 X MGC80-3.27 X MGC80-3 28 29 29 30 31 32 33 X MGC80-3 34 X..34.35.36
More informationLotus Notes Design and Implementation of Sales Process Management System Based on Lotus Notes 施莹艳 指导教师姓名 : 林林林林坤坤坤坤辉辉辉辉 教授 专 业 名 称 : 软软软软件件件件工工工工程程程程 论论论论文文文文提提提提交交交交日日日日期期期期 : 年 论论论论文文文文答答答答辩辩辩辩时时时时间间间间
More informationJ. D. 17 Daniel J. Elazar, American Federalism: A View From the States (New York: Happer & Row, Publishers, 1984), p
20 20 70 80 1 20 90 20 90 J. D. 17 Daniel J. Elazar, American Federalism: A View From the States (New York: Happer & Row, Publishers, 1984), p.41-42. 2 19 1985 80 Gerald D. Nash, State and Local Governments,
More informationWTO WTO ATM POS 4 CRM 2
Carl W.Buechner 2002 9 + CRM CRM 1 CRM Hans Peter Brondmo 2002 2 CEO 1 2 Hans Peter Brondmo 2002 1 WTO WTO 1 1000 2 3 ATM POS 4 CRM 2 1 2 3 4 Thibaut & Kelley Rusbult Polanyi Thibaut & Kelley 1959 Rusbult,1980
More information10384 9702001 UDC 2000 5 2000 6 2000 2000 5 1 .1 1 2 3. 4 4 4.5.6 6 1..7 2..7. 8 8 1..8 1..8 2 10 2 11..12 1 12 1 12 2 12 2 12 1 12 2 13 3 13 4 14 3 14 4 14..16..17..19..20..21 2 3 1 20 1921 1926 1928
More information100Mbps 100Mbps 1000Mbps 100Mbps 1000Mbps 100Mbps 100Mbps PD LXT Mbps 100Mbps 100Mbps 1
1 3 6 6 7 100BASE-FX 10 100Mbps 13 13 21143-PD 14 LXT970 20 Serial ROM 24 Boot ROM 24 Agilent 25 100Mbps 27 27 37 100Mbps 45 50 54 55 100Mbps 100Mbps 1000Mbps 100Mbps 1000Mbps 100Mbps 100Mbps 21143-PD
More information...12...12...13...18...18...24 MSCI...27 FTSE...29...33...36................................................... 1 ...........................39...39...42...45...54.............................................
More information- 2 - Russell Thaler 2 1 2 unexpected dramatic P t =P t-1 + P t t P t-1 t-1 2 T.Russell and R.Thaler, The Relevance of Quasi-Rationality in Competitiv
- 1-1 Bayes 1 1 A B P AB =P A *P B - 2 - Russell Thaler 2 1 2 unexpected dramatic P t =P t-1 + P t t P t-1 t-1 2 T.Russell and R.Thaler, The Relevance of Quasi-Rationality in Competitive Markets. American
More informationSWOT Abstract Abstract Fuzhou Water & Wastewater Engineering Design Institute is an affiliate project prospecting and designing unit under Fuzhou Water Supply General Company. It engages mainly in the
More information2.
1 20 60 < > < > 2. 3 1 1.Davis,John Francis,"The Poetry of Chinese" Journal of The Royal Asiatic Society of Great Britian and Ireland 11(1830),(Translation of chapter 3 of the novel) 1830 1792 2.Tom Rorbert,"The
More information1
10384 199819007UDC 2 0 0 2 4 2 0 0 2 6 2 0 0 2 2 0 0 2 6 1 2 1 1992 1 3 1 1 1 3 6 6 22 25 26 4 145 78 42 113 o 31 36 113 o 35 43 22 o 06 39 22 o 13 06 2.5 2 25.66 8.61 6.54 7.56 2.95 2 40000 32500 32500
More informationResearch on the Mycorrhizal Community of Pinus Massoniana Lamb in Wuyishan Nature Reserve Abstract Mycorrhizae is the symbiosisal body of fugus and bo
(pinus massoniana Lamb) 27 o 33. o 54, 117 o 27 117 o 51 1 Research on the Mycorrhizal Community of Pinus Massoniana Lamb in Wuyishan Nature Reserve Abstract Mycorrhizae is the symbiosisal body of fugus
More information2002 II
10384 B9901001 UDC A Grammatical Study Of Wanrong Dialect 2OO2 I 2002 II III Abstract The thesis is intended to make a more systematic study of the grammar on Wanrong dialect, which is distributed in the
More informationResearch for RS encoding and decoding technology in the Digital Television Terrestrial Broadcasting System 2006 厦门大学博硕士论文摘要库
Research for RS encoding and decoding technology in the Digital Television Terrestrial Broadcasting System 2006 DMB-T RS DMB-T DMB-T RS(208,188) RS BM RS : 1 RS RS RS FPGA RS 2 RS 3 BM 4 matlab RS RS
More information<4D6963726F736F667420576F7264202D20B2CCA3BA4542B2A1B6BE52746120496747BFB9CCE5D3EBB1C7D1CAB0A9B7D6C6DAB5C4B9D8CFB52E646F63>
EB 病 毒 Rta/IgG 抗 体 与 鼻 咽 癌 分 期 的 关 系 蔡 永 林, 郑 裕 明, 成 积 儒, 李 军, 莫 永 坤, 钟 青 燕 ( 广 西 梧 州 市 红 十 字 会 医 院, 梧 州 市 肿 瘤 防 治 研 究 所, 广 西 梧 州 543002) 摘 要 : 目 的 探 讨 EB 病 毒 Rta/IgG 抗 体 与 鼻 咽 癌 临 床 分 期 的 关 系 方 法 用 酶
More informationKluyveromyces sp. Y-85 Saccharomyces cerevisiae E-15, E g/100ml Y-85 E-15 DNA Y YEPD MM E E-15 Y-85 Y-85 12h E h 0.1%ED
10384 9626012 UDC 1999 7 1999 7 16 1999 1999 7 1 Kluyveromyces sp. Y-85 Saccharomyces cerevisiae E-15, E-15 10 2.98g/100ml Y-85 E-15 DNA Y-85 45 YEPD MM E-15 45 30 E-15 Y-85 Y-85 12h E-15 10 h 0.1%EDTA-,
More information- 2 -
2002 11 10384 9915064 UDC Money Trapping By the Listed Companies and Its Prevention MBA 2002 11 2002 12 2002 - 2 - Abstract In the first place, this paper defines the conduct of some listed companies which
More informationSuperoxide dismutase,sod (O 2. ), SOD O 2., SOD psod SODhCu,Zn-SOD, Cys 111 Ala 111 groesl rbcs polya Kan r pesodt111 Synechococcus sp.pcc7942 groesl
CATALOGUE Abstract 1 1. Preface 1. 1 SOD Brief introduction 5 1. 2 Human SOD 14 1. 3 The study of cyanobacterium gene engineering19 1. 4 The purperse and meaning of the study 25 2. Material and methods
More information10384 200126008 UDC Isolation of microsatellite markers from Porphyra haitanensis and their application in genetic diversity of Porphyra 2004 10 10 2004 10 13 2004 10 ...1...3...5 1...5 2...6 2.1...6
More informationAbstract / / B-ISDN ATM Crossbar Batcher banyan N DPA Modelsim Verilog Synopsys Design Analyzer Modelsim FPGA ISE FPGA ATM ii
10384 200024024 UDC 2003 5 2003 6 2003 2003 5 i Abstract / / B-ISDN ATM Crossbar Batcher banyan N DPA Modelsim Verilog Synopsys Design Analyzer Modelsim FPGA ISE FPGA ATM ii System On-Chip Design and Performance
More informationUDC The Establishment of Fractional BSDE : : : : : 厦门大学博硕士论文摘要库
10384 19020071152070 UDC The Establishment of Fractional BSDE : : : 2010 4 : 2010 5 : 2010 6 2010 4 1. 2. 1973 1990 Pardoux. Duffie Epstein ( ) Feynman-Kac Navier-Stokes El Karoui Quenez ( )... - i Abstract
More informationuntitled
01 1-1 1-2 1-3 1-4 1-5 1. 2. 3. 1-1 (epidemiology) epidemiology epi(among) demos (people) logos(doctrine) 2 01 1. (epidemic) 2. (endemic) 3. (pandemic) (Severe Acute Respiratory Syndrome, SARS) 1-2 1.
More information.. Abstract
10384 985007 UDC 001.8 001.8 001 8 .. Abstract... 1 1.1.1 1...1 1.3.. 1.4..3.6.1.6..7..1...7.....8..3...8..4...10..5.11.3...1.3.1.1.3..13.3.3.13.3.4..17.3.5.19.4...0.5...6 9 3.1...9 3....30 3..1.31 3...31
More information医学科研方法
医 学 科 研 方 法 主 讲 苏 秀 兰 细 胞 生 物 学 教 授 第 一 讲 绪 论 一 概 念 : 在 医 学 科 学 研 究 活 动 中, 运 用 科 学 的 实 践 和 理 论 思 维 的 技 巧, 充 分 发 挥 自 己 的 智 慧, 去 寻 找 观 念 世 界 与 现 实 世 界 之 间 的 联 系 或 规 律, 从 而 发 现 新 现 象, 提 出 新 理 论 的 手 段 二 医
More information<453A5CCAB3C6B7BFC6D1A73131C4EA3134C6DA5C3134C6DA5C3032B7D6CEF6>
227 ELISA 刘 沙 洲 1,2, 桑 小 雪 1, 欧 阳 华 学 3, 雷 绍 荣 3, 白 林 含 1, * (1. 四 川 大 学 生 命 科 学 学 院, 四 川 成 都 610065;2. 成 都 市 食 品 药 品 检 测 中 心, 四 川 成 都 610045; 3. 四 川 省 农 业 科 学 院 分 析 测 试 中 心, 四 川 成 都 610066) 目 的 : 比 较
More informationUDC Design and Application of Intrusion Detection System Based on Snort 2009 12 Snort Snort Apriori Snort Apriori Microsoft Visual Studio 2008 C# Snort Snort Snort Snort Snort Apriori Snort Snort Apriori
More information2
10384 9926013 UDC : 2002 8 2002 8 2002 2002 8 1 2 3 µ µ Abstract Hepatitis B virus infection is one of the most widespread viral infections of humans. China belongs to high epidemic area. Chinese government
More information南華大學數位論文
南 華 大 學 ( 文 學 所 ) 碩 士 論 文 論 文 題 目 ( 陳 千 武 小 說 活 著 回 來 及 其 相 關 事 例 研 究 ) 論 文 題 目 (Chen Chien Wu Return Alive And Some Research About It) 研 究 生 : 朱 妍 淩 指 導 教 授 : 林 葉 連 中 華 民 國 一 0 一 年 6 月 8 日 陳 千 武 小 說
More information<4D6963726F736F667420576F7264202D20ECFAD0C0D0C0C2DBCEC4CCE1BDBB6F7665722E646F63>
硕 士 专 业 学 位 论 文 论 文 题 目 鲍 温 病 临 床 病 理 特 征 和 几 种 治 疗 方 法 比 较 的 分 析 研 究 研 究 生 姓 名 指 导 教 师 姓 名 禚 欣 欣 康 定 华 专 业 名 称 皮 肤 性 病 学 研 究 方 向 皮 肤 肿 瘤 与 皮 肤 病 理 论 文 提 交 日 期 2013 年 4 月 I 鲍 温 病 临 床 病 理 特 征 和 几 种 治 疗
More information10384 200129005 UDC _ Product Lines and their Control System Design for Fine Powder and Ultra-fine Powder 2004 5 2004 6 2004 2004 5 AUTOCAD PLC PC PLC PC PLC PLC VB6.0 PLC VB PID I MATLAB PID PLCCPU PID
More informationSARS综述_临床版.doc
SARS ( ) 2002 11 Severe Acute Respiratory Syndrome SARS 2003 5 14 SARS 32 7548 573 ( 1) [1] SARS SARS 1 SARS May 14, 2003. available at http://www.who.int/csr/sars/map2003_05_14.gif 1 SARS 2002 11 2003
More information主 要 在 密 閉 空 間 中 經 由 感 染 者 咳 嗽 或 打 噴 嚏 產 生 的 飛 沫 傳 染 ; 由 於 流 感 病 毒 可 在 寒 冷 低 溼 度 的 環 境 中 存 活 數 小 時, 故 亦 可 能 經 由 接 觸 傳 染 六 潛 伏 期 (Incubation period) 流 感
流 感 併 發 重 症 (Severe Complicated Influenza) 一 疾 病 概 述 (Disease description) 流 感 為 一 種 急 性 病 毒 性 呼 吸 道 疾 病, 感 染 後 可 能 出 現 發 燒 頭 痛 肌 肉 痛 疲 倦 流 鼻 涕 喉 嚨 痛 以 及 咳 嗽 等 症 狀, 大 多 數 人 通 常 可 在 兩 週 內 完 全 康 復 然 而, 有
More information530 军 杂 ~8d 4.2 ( ) ( 37.5 ) % 38.1~ % 37.3~ % ( ) 1 14d( 5 7d) % 40% 38.5 X CT 5.1
Med J Chin PLA, Vol. 38, No. 7, July 1, 2013 529 [ ] R511.8 A 0577-7402(2013)07-0529-06 2011 12 B 55 7 14 B1 C E B2 [1-8] DNA 34.7kb 20 80~110nmDNA 252 240 12 56 30min [9] A G 7 551 8 55 11 14 B B2 [10-11]
More informationSARS-CoV 1-3 RNA HCoV-229E 1 HCoV-OC SARS-CoV 5 [rep] [S] [E] [M] [N] 3 rep 2/3 rep 4 S E M N 1 SARS GenBank 16 2 IBV IBV BCK 4 BCo
* 100850 2003 3 SARS-CoV 2002 Severe Acute Respiratory Syndrome SARS SARS-CoV SARS-CoV 5 S E M N SARS-CoV SARS-CoV IBV TGV IBV E M SARS Severe Acute Respiratory Syndrome 2002 * 30080017,30270315 1 2003
More information國立中山大學學位論文典藏.PDF
( ) 2-1 p33 3-1 p78 3-2 p79 3-3 p80 3-4 p90 4-1 p95 4-2 p97 4-3 p100 4-4 p103 4-5 p105 4-6 p107 4-7 p108 4-8 p108 4-9 p112 4-10 p114 4-11 p117 4-12 p119 4-13 p121 4-14 p123 4-15 p124 4-16 p131 4-17 p133
More informationOrigination And Filmization: Research Of Relation Between The Modern Chinese Movie And literature 2005 7 21 I II Abstract Movie has been knotted with literature tightly since the day of naissance. The
More information10384 200101021200101021 UDC Literature Rectification:19421952 A Research on Relationship between Yan an Literature and Seventeen Literature 2004 6 2004 6 2004 6 2004 6 19421952 19421952 I II 19421952
More information: 23 S00017242 1 -----------------------------------------------------------------------------1 -----------------------------------------------------------------------------3 -------------------------------------------------------------------7
More information厦门大学博硕士论文摘要库
10384 B200009005 UDC 2003 4 2003 2003 2003 4 20 80 20 80 80 Abstract The historical-cost position of foreign direct investment in the United States (FDIUS) accounts for the largest share of world FDI outward
More informationMicrosoft Word - 360145.doc
项 目 批 准 号 81273814 归 口 管 理 部 门 申 请 代 码 收 件 日 期 H2716 360145 y 国 家 自 然 科 学 基 金 委 员 会 资 助 项 目 计 划 书 资 助 类 别 : 面 上 项 目 亚 类 说 明 : 非 连 续 资 助 类 项 目 附 注 说 明 : 项 目 名 称 : 益 气 养 肺 法 拮 抗 bfgf 对 肺 癌 微 环 境 介 导 化 疗
More information第一章 出口退税制改革的内容
密 级 学 号 2 0 0 1 0 3 2 9 毕 业 设 计 ( 论 文 ) 出 口 退 税 制 改 革 对 我 国 出 口 的 影 响 院 ( 系 部 ): 经 济 管 理 学 院 姓 名 : 王 晓 年 级 : 2001 级 专 业 : 国 际 经 济 与 贸 易 指 导 教 师 : 杜 秀 芳 教 师 职 称 : 讲 师 2005 年 6 月 10 日 北 京 北 京 石 油 化 工 学 院
More information