Microsoft Word 舒琥_new_.doc

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1 46 2 Vol.46, No OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2015 (Epinephelus lanceolatus) (Epinephelus coioides) * ( ; ) (Epinephelus lanceolatus) (Epinephelus coioides), (2n=48) (3n=72),, 3 : 2;, ;, / 0.79,, / 0.69, ;,,, ; ;, ; ; ; ; S doi: /hyhz (Epinephelus lanceolatus), (Epinephelus coioides), (Heemstra et al, 1993; Nelson, 2006),,,,,,,,,,,,,,,,,,,,, (, 2010),,, ( 8%),,, *, ;, ;, B ,,, shuhu001@126.com :,, zhhaifa812@hotmail.com : , :

2 2 : (Epinephelus lanceolatus) (Epinephelus coioides) 397 (, 2012;, 2013;, 2014),,,,, 1 1.1,,, ( ±622.49)g, (34.50±8.06)cm, ( ± )g, ( )cm % EDTA 1mL,, MS- 222 (100 mg/l)( ) : : 0.8%, 200, 50μL EDTA,,, OLYMPUS-BX51, 4 :,,, 1min, 5 10min,, OLYMPUS-BX51 10, 50, : 10,, 50 S = πa b/4 V = a 2 b/1.91 (a, b )(, 2009) C 4 6h, 4 C r/min 5 8min,, 7170A Excel SPSS 17 (One-Way ANOVA), Duncan t, P<0.05, P<0.01 ± (Mean±SE) , 1, 表 1 二倍体与三倍体青龙斑血液常数值的比较 Tab.1 Comparison of haematological parameters between diploid and triploid Qinglong groupers 3n/2n (WBC) ± ± 14.14** 0.94 G/L (RBC) 0.45 ± ± 0.10** 0.62 T/L (HGB) ± ± 3.99** 0.74 g/l (HCT) 2.05 ± ± 0.78* 1.08 % (PLT) ± ± 10.07** 0.85 G/L (PCT) 0.03 ± ± % (MCV) ± ± 12.37** 1.28 fl (RDW) ± ± 3.09** 1.12 %CV (MPV) 6.18 ± ± 1.07** 1.04 fl (PDW) ± ± 2.48* 1.23 %

3 398 46,,, ; 2.2 2, /L, /L, 1.52 表 2 二倍体与三倍体青龙斑红细胞数量比较 Tab.2 Comparison on amount of erythrocyte in diploid and triploid Qinglong groupers 3n/2n ( ) /L ( ) /L** , 3, t,, (P<0.01); 1.52, 1.59, (P<0.05) / 0.69, 0.79,, 表 3 二倍体与三倍体青龙斑中红细胞和细胞核大小的对比 Tab.3 Comparison in the size of red blood cell and nucleus in diploid and triploid Qinglong groupers 3n/2n (μm) ± ± 1.18** 1.23 (μm) 8.36 ± ± (μm 3 ) ± ± 67.97* 1.52 (μm 2 ) ± ± 7.08** 1.22 (μm) 5.35 ± ± 0.76** 1.28 (μm) 4.20 ± ± 0.55** 1.12 (μm 3 ) ± ± 12.83** 1.59 (μm 2 ) ± ± 4.65** 1.43 / , 4,,, 表 4 二倍体与三倍体青龙斑白细胞大小比较 Tab.4 Comparison in size of leukocyte in diploid and triploid Qinglong groupers (μm) 3n/2n (μm) 6.40 ± ± (μm) 6.11 ± ± (μm 3 ) ± ± (μm) 8.33 ± ± 0.30* 1.20 (μm) 6.27 ± ± 0.62** 1.15 (μm 3 ) ± ± 18.75** 1.59 (μm) 8.50 ± ± (μm) 7.59 ± ± (μm 3 ) ± ± (μm) ± ± 1.09** 1.25 (μm) 6.69 ± ± 0.97* 1.14 (μm 3 ) ± ± 16.73** 1.58

4 2 : (Epinephelus lanceolatus) (Epinephelus coioides) ,,,,,,,,,, ( 1a, 1b);,,,,,,,, ( 1c, 1d),,,, ( 1e, 1f),,,, ( 1g, 1h),,, ( 1i, 1j), ( 1s), ( 1k, 1l),,,,,, : ( 1m, 1n),, 1/3,,,,,,, : ( 1o, 1p),,,, 2 3,, : ( 1q, 1r),,,,,, , t, ; , DNA, (Gregory, 2002), 1.5 (.. = 3..2), , 1.52, (, 2010),,,,, (Stefano et al, 2005; Gao et al, 2007; Del et al, 2011),,, (Small et al, 1987; Sezaki et al, 1988, 1991; Benfey, 1999; Stefano et al, 2005; Gao et al, 2007;, 2011) : (1),, ; (2),,,,,,, (2004) ;, (, 2007),,

5 400 海图1 Fig.1 洋与湖沼 46 卷不同倍性的青龙斑血细胞光镜照片 Microscopic images of blood cells in Qinglong groupers of different ploidies a. 二倍体原红细胞, 箭头示原红细胞; b. 三倍体原红细胞箭头示原红细胞; c. 二倍体幼红细胞, 箭头示幼红细胞; d. 三倍体幼红细胞箭头示幼红细胞; e. 二倍体成熟红细胞, 箭头示成熟红细胞; f. 三倍体成熟红细胞, 箭头示成熟红细胞; g. 二倍体衰老红细胞, 箭头示衰老红细胞; h. 三倍体衰老红细胞, 箭头示衰老红细胞; i. 二倍体血影细胞, 箭头示血影细胞; j. 三倍体血影细胞, 箭头示血影细胞; k. 二倍体淋巴细胞, 箭头示淋巴细胞; l. 三倍体淋巴细胞, 箭头示淋巴细胞; m. 二倍体嗜中性粒细胞, 箭头示嗜中性粒细胞; n. 三倍体嗜中性粒细胞, 箭头示嗜中性粒细胞; o. 二倍体嗜酸性粒细胞, 箭头示嗜酸性粒细胞; p. 三倍体嗜酸性粒细胞, 箭头示嗜酸性粒细胞; q. 二倍体单核细胞, 箭头示单核细胞; r. 三倍体单核细胞, 箭头示单核细胞; s. 三倍体异常红细胞所有子图均为 400, 箭头示异常红细胞

6 2 : (Epinephelus lanceolatus) (Epinephelus coioides) 401 表 5 二倍体和三倍体青龙斑生化指标比较 Tab.5 Comparison in biochemical index between diploid and triploid Qinglong groupers 3n/2n (ALT) ** 0.64 U/L (AST) U/L / (AST/ALT) ** 4.07 (TP) * 1.33 g/l (ALB) ** 1.36 g/l (GLB) ** 1.38 g/l ALB/GLB (GGT) U/L (ALP) U/L (TBA) * 1.27 umol/l (Urea) ** 1.42 mmol/l (Cr) ** 7.5 umol/l (UA) ** 0.22 umol/l - (GLU) mmol/l (TG) ** 1.56 mmol/l (GPO-PAP) (TC) mmol/l (CHOO-PAP) (HDL-C) * 0.83 mmol/l (LDL-C) mmol/l B(Apo-B) g/l (Na + ) mmol/l (Cl - ) * 0.85 mmol/l (K + ) ** 0.47 mmol/l (Ca) mmol/l - (P) * 0.84 mmol/l,,, 3.2, (MCV)(HCT), (Stefano et al, 2005), (Gao et al, 2007), (1996),,,,,,, ; ;,, (1982), : (1) :, mmol/L ;,,, (2) :, mmol/L;, 10mmol/L;,, 5mmol/L ;

7 402 46, ;,,, , (02): 74,,, , 36(6): ,,, , 41(11): ,,, , 35(10): ,,, (), 1: ,,, , (01): 86 92,,, , 42(3): , :, 89 94,,, , 36(2): , :, 129,,,, :, Benfey T J, The physiology and behaviour of triploid fishes. Rev Fish Sci, 7: Del O B, Fajer-Avila E J, Alamazan-Rueda P et al, Hematological characteristics of the spotted rose snapper Lutjanus guttatus (Steindachner, 1869) healthy and naturally infected by dactylogyrid. Tissue and Cell, 43: Gao Z X, Wang W M, Khalid A, Haematological characterization of loach Misgurnus anguillicaudatus: Comparison among diploid, triploid and tetraploid specimens. Comparative Biochemistry and Physiology, Part A, 147: Gregory T R, Genome size and developmental parameters in the homeothermic vertebrates. Genome, 45(5): Heemstra P C, Randall J E, Groupers of the world. FAO Fisheries Synopsis, 16(125): Nelson J S, Fishes of the World. 4 th ed. New York: Wiley Press, Sezaki K, Watabe S, Hashimoto K, Haematological parameters and erythrocyte enzyme activities associated with increase in ploidy status of the spinous loach, Cobitis biwae Jordan and Snyder. J Fish Biol, 32: Sezaki K, Watabe K, Tsukamoto K et al, Effects of increase in ploidy status on respiratory function of ginbuna, Carassius auratus Langsdorfi (Cyprinidae). Comp Biochem Physiol A, 99: Small S A, Benfey T J, Cell size in triploid salmon. J Experi Zoology, 241: Stefano P, Stamatis V, Beatrice C, Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. Aquaculture, 244: COMPARATIVE STUDY ON HAEMATOLOGY AND PHYSIOLOGY OF EPINEPHELUS COIOIDES ( ) EPINEPHELUS LANCEOLATUS ( ) HYBRID F 1 QINGLONG GROUPER SHU Hu 1, YANG Li-Dong 1, LI Yan 1, LI Xiao-Mei 1, HOU Li-Ping 1, WANG Ji 1, ZHANG Hai-Fa 2 (1. School of Life Science, Guangzhou University, Guangzhou , China; 2. Guangdong Daya Bay Fisheries Development Center, Huizhou , China) Abstract To determine whether diploid and triploid hybrid F 1, the Qinglong grouper (Epinephelus coioides Epinephelus lanceolatus ) differed in haematology and physiology, we studied the morphological features and biochemical parameters of the blood cells in light microscopy of Wright-Giemsa-stained blood films with biochemical analytical equipment. The result shows that the blood cells were significantly larger in triploid, and the volumetric ratios of erythrocytes and leucocytes between triploid and diploid grouper were The erythrocytes count decreased in triploid, and the mean thrombocyte volume haemoglobin more significantly decreased in triploid grouper than in diploids. The mean cell volume significantly increased in triploid grouper. Triploids showed lower concentration of K, P, and Cl than their diploid counterparts. In blood cell morphology, diploid red blood cells are more rounded, while triploid erythrocytes are oval with many atypical cells. Therefore, biochemical difference between diploid and triploid groupers were remarkable. Key words Qinglong grouper; diploid; triploid; blood cell; blood biochemical index

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