2011Q1 January-March 騰達行企業股份有限公司 UNIMED HEALTHECARE INC

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1 2011Q1 January-March 騰達行企業股份有限公司 UNIMED HEALTHECARE INC Arg Asp Ala Gln Glu Phe Gly Asn 超級 Cys Leu Ile Trp Pro Tyr Ser Met 蛋白質表現 純化與樣品製備 Ala Lys Asn Ile Leu 陣容 Asp Gln Phe Ala Asn Tyr Ser Leu Pro Ile Asn Ile Ala Glu Leu 新品上市 本期焦點 全部搞定! 同時 microrna 研究小幫手萃取定量觀察 HUVEC 細胞 ~ 研究 angiogenesis 的最佳工具 BD 輕鬆測得抗原專一性免疫反應 不需固定細胞週期立即現形 P.34

2 NEW Collagenase & Neutral Protease 組織分離的好幫手 lot-to-lot consistency Low endotoxin TSE (Transmissible Spongiform Encephalopathy) safe manufacturing Research Grade GMP Grade, Research Grade GMP Grade Collagenase protocol Description Collagenase NB 4 Standard Grade Collagenase NB 4G Proved Grade Collagenase NB 5 Sterile Grade Collagenase NB 6 GMP Grade Collagenase NB 8 Broad Range Collagenase NB1 GMP Grade Collagenase NB1 Premium Grade* Neutrale Protease GMP Grade* Neutrale Protease NB* Application isolation of a broad variety of cells especially suitable for isolation of adipocytes, fibroblasts, hepatocytes, frog oocytes, chondrocytes and stem cells isolation of a broad variety of cells, produced under aseptic condition according to the European Pharmacopoeia isolation of a broad variety of cells, stem cells and stem cell passaging for human transplantation isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets, tumor and thyroid cells isolation of pancreatic islets for human transplantation isolation of pancreatic islets cell isolation in combination with Collagenase NB cell isolation in combination with Collagenase NB GMP Grade,

3 2011. January - March Q1 P2 蛋白質表現 純化及樣品製備的超級陣容 陣容 1. 2 陣容 2. Co2+ Ni2+ His-Tag 7 陣容 全部搞定! 同時研究 microrna 小幫手萃取定量觀察 14 蛋白質表現 E. Coli Brevibacillus Baculovirus His tag 蛋白純化 Cobalt charged TALON P10 Nickel charged His60 夯 HUVEC 細胞 ~ angiogenesis 20 Q & A 21 Signaling Transduction ~ 22 輕鬆測得抗原專一性 T 細胞免疫反應 ELISOPT 24 DimerX I MHC-Ig P22 CBA Flex Set Femtogram 等級的 Cytokine 偵測試劑 New 28 細胞週期分析套組 P24 試驗方法二 FluorChem 32 試驗方法三 33 試驗方法一 新品介紹 不需固定, 細胞週期立即現形 34 Neptune UNIMED HEALTHCARE INC. All Rights Reserved

4 Clontech Takara 超級 純化家族的誕生 蛋白質表現 E. Coli Brevibacillus Baculovirus 陣容 Cobalt charged TALON His tag 蛋白純化 Nickel charged His60 E. Coli expression system pcold expression system E. coli,,,, Cold shock protein TaKaRa pcold Cold shock protein CsA Promoter, T7 lac operon, IPTG pcold : 60%, : E. coli, pcold, E. coli Chaperone Plasmids, Radioisotope Labeling : 90% 蛋白質表現 使用 pcold 可溶蛋白增加 Pulse labeling 產物幾乎全為重組蛋白 January - March Q1 Target T7 pcold Human gene C ( 80 kd) Total lysate (T) Soluble fraction (S),SDS-PAGE pcold Time after Induction T7 pcold Pluse labeling, pcold,, 02 台北 新竹 台中 嘉義 台南 高雄 服務專線

5 5 種 pcold 載體供您選擇 Features of Takara's pcold Vectors Catalog No Vector TEE His-Tag Factor Xa Cleavage Site 3361 pcold I DNA yes yes yes 3362 pcold II DNA yes yes no 3363 pcold III DNA yes no no 3364 pcold IV DNA no no no 3365 pcold TF DNA yes yes yes 1:pCold I-IV 2:pCold TF Folding pcold TF Trigger factor (Prokaryotic ribosome-associated chaperone protein, 48 kda) Target protein, pcold TF His tag, TF Target protein Thrombin Factor Xa, TF Clontech Takara pcold I 載體 MAP pcold TF 載體 MAP Chaperone Plasmid Set (#TAK 3340) 5 plasmid set, chaperone, folding E. Coli expression system * * pet pcold, BL21(DE3) plyss BL21(DE3) plyse Refolding CA Kit (#TAK 7350) : Chaperone,Cycloamylose (CA) refolding, 2-step protocol Chaperone 家族在 protein folding 可能扮演的角色 January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

6 Clontech Takara 純化家族的誕生 Brevibacillus expression system 蛋白質表現 No Endotoxin:, Safe Organism:, Low Protease Activity:,, Correct Folding:, folding High Level of Secretory Protein: Media, * (E. Coli ) Express Intracellular protein without forming Inclusion Brevibacillus 重組蛋白活性高, 商業價值高 CSIRO, Brevibacillus Human EGF ( EGF ),,, ( ) : 胞外表現 Supernatant Precipitate cation Eliminate the his tag conc. Low High His-tagged BLA (57.2 kda) Marker (- control) M : Marker 1 : purified His-tagged BLA 2-5 : Thrombin cleaved January - March Q1 胞內表現 pet Brevibacillus Dehydrogenase,Brevibacillus E. Coli Dehydrogenase Protein expression using E. coli BL21(DE3) after 3 and 16 hours of induction in soluble fraction as well as precipitate. M: Molecular weight marker. C: pet-15b (control). 1: pet- 15b-DH. sol: soluble fraction. ppt: precipitate. Protein expression using B. choshinensis in 2 diff erent media (TMNm & 2SLNm) in soluble fraction as well as precipitate. M: Molecular weight marker. C:pNI (control). 1-2: pni-dh. sol: soluble fraction. ppt: precipitate. D H a c t i v i t y m e a s u r e m e n t o f p r o t e i n expressed, using the Brevibacillus expression system (in 2 diff erent media) or using E. coli (after 16 hour induction or no induction). 04 台北 新竹 台中 嘉義 台南 高雄 服務專線

7 載體資訊 Clontech Takara Extracellular Intracellular 成功範例 ( 皆有文章發表 ) Proteins Origins Production( g/l ) Enzymes α-amylase B. licheniformis 3.7 Sphingomyelinase B. cereus 3 Xylanase B. halodurans 0.2 CGTase B. macerans 1.5 Chitosanase B. circulans 1.4 Hyper thermo-stable protease A. pernix 0.1 Hyper thermo-stable nuclease P. horikoshii 0.7 PDI human 1 Antigens Surface antigen E. rhusiopathiae 0.9 Surface antigen T. pallidum 0.8 Cytokines EGF human 2 IL-2 human 0.6 NGF mouse 0.2 IFN- γ chicken 0.5 TNF-α bovine 0.4 GM-CSF bovine 0.2 GH flounder 0.2 訂購資訊 Product Name Catalog No. Package Brevibacillus experession system HB100 1 Kit 1. pncmo2 2. pny pncmo2-bla 4. B. Choshinesis Eletro-cells Brevibacillus choshinesis Eletro-cells HB ul x 10 pnc-hise HB ug pnc-hisf HB ug pnc-hist HB ug pncmo2 HB ug pni HB ug pni-his HB ug January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

8 Clontech Takara 純化家族的誕生 Baculovirus expression system 昆蟲表現系統 ( mg/ Liter cells) BacPAK recombination arm, ( >90%) : 1) 2) 3) 4) 5) Clone (~8 Kb) In-Fusion Ready Vector 蛋白質表現 ~ 三種常使用於定量 Baculovirus 的方法 Recombinant baculovirus efficiently transduces insect cells, allowing high-level expressionof 6xHN-tagged AcGFP1. Monolayers of Sf21 cells were infected with either 6xHN-AcGFP1 (Panels Aand C) or AcGFP1-6xHN (Panels B and D) at an MOI of 5 for 46 hr. The cells were then visualized by phase-contrast microscopy (Panels A and B) and fluorescence microscopy (Panels C and D) using a Zeiss Axioskop microscope equipped with a filter for green fluorescent protein (GFP). As shown by the high levels of 6xHN-AcGFP1 expression in Panels C and D, virtually all of the insect cells were transduced by recombinant baculovirus. Comparison titration methods Description Time Benefits Plaque Assay Count cleared plaques in infected cell monolayer 1 week visual BacPAK Rapid Titer Assay BacPAK qpcr Titration Immunostaining of Gp64 in infected cell monolayer SYBR-based qpcr to measure viral DNA 48 hr 2-4 hr Simple, visual Fast, accurate Baculovirus 小學堂 January - March Q1 Baculovirus AcMNPV ( Autographa californica multiple nucleopolyhedro virus),viral genome 130 kb, (~8kb),, protein targeting,,,, Baculovirus linear DNA transfer vector, Homologous recombination,, Baculovirus linear DNA, shear stress DNA, DNA,,,, chemical defined serum free media (SFM) plaque assay,,, SFM,, 訂購資訊 BacPAK 6 DNA (Bsu36 I digest) 5 transfxns 5 transfxns BacPAK Baculovirus Expression System each each BacPAK Baculovirus Rapid Titer Kit 5 assays 5 assays In-Fusion Ready BacPAK Vector Set 3 vectors 3vectors IPLB-Sf21 Insect Cells 1 vial 3 vials BacPAK qpcr Titration Kit 200 rxns BacPAK 系列全面 85 折 現在買 BacPAK Linear DNA 或 Titration kit 再加 388 元即可獲得 LONZA Insect-Xpress Serum Free Medium (500 ml)! 06 台北 新竹 台中 嘉義 台南 高雄 服務專線

9 IMAC purification system His tag 蛋白純化 IMAC (immobilized metal affinity chromatography) Histidine Side chain, Poly-histidine,, Elution,,Elution, : > >, : > >,, Clontech Takara 螯合劑 IDA NTA TALON TED Ni Ni2+ His tag IDA NTA TALON TED IDA NTA TALON, TED,, TED, His Tag His tag :IDA > TALON = NTA >TED, Leakage,, Leakage ( ) NTA TALON, NTA, TALON 3D,, NTA Leakage TALON,,IDA Leakage, TALON 四種常見螯合劑 Clontech TALON & His60 全系列下殺 單筆訂單滿額 85 折 再加碼滿 10,000 萬 滿 30,000 萬 贈 Protease inhibitor cocktail (500 ul) 一支 (# IN0902-1) 贈彩色 Protein marker (250 ul) 一支 (# IN1124) January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

10 Clontech Takara Clontech TALON Resin 純化家族的誕生 TALON 3D, (TALON ), Leakage, His tag,, TALON Resin His tag Cobalt Resin TALON,Leakage His tag, ( ) Native purification TALON 較 Ni-NTA 更具專一性 ( 純化速度也較快 ) IMAC purification TALON vs. Ni-NTA. 6xHN- AcGFP1 was purified from Sf21cells using TALON or Ni-NTA. The chromatogram for each column is shown. The absorbance at 475 nm indicates the amount of target protein (AcGFP1) present in each fraction. 比較 TALON 及 Ni-NTA 在 Native condition 下蛋白質回收量 January - March Q1 TALON 對還原劑耐受性明顯高於 Ni-NTA Purification of polyhistidine-tagged proteins under native conditions compared to purification using Ni-NTA. Purification, yields and SDS-PAGE fractions of protein purified with TALON Resin in the presence of β-mercaptoethanol. 08 台北 新竹 台中 嘉義 台南 高雄 服務專線

11 Clontech His60 Superflow Resin Nickel Resin IDA, Leakage, (60 mg/ml), leakage binding capacity ~60 mg/ml Nickel Resin Clontech Takara His60 與 Ni-NTA 的差異 Superior yields and better purity were obtained when using His60 Ni Superflow for purificationas compared to Competitor Q'sNi resin. Clontech's pecoli Linear Expression System was used to express 6xHN-AcGFP1 in E. coli. Equivalent amounts of the same sample were used for comparing His60 Ni Superflow Resin purification (Panel A) with Competitor Q Ni Resin purification (Panel B). Lane M: Molecular weight marker. Lane OS: Original sample. Lane FT: Flowthrough. Lanes W1 and W2: Wash. Lane E1E4: Eluted 6xHN-AcGFP1 fractions. 訂購資訊 His60 Superflow Nickel Resin Description Package Catalog No 10 ml His60 Ni Superflow Resin 25 ml ml ml His60 Prepacked Column Description Package Catalog No Prepacked Column Bulk Resin His60 Ni Gravity Columns 5 columns His60 Ni Gravity Purification Kit 20 purifications His60 Ni Superflow Cartridges 5 x 5 ml His60 Ni Superflow Cartridge 1 x 5 ml TALON Cobalt Resin TALON Prepacked Column Description Package Catalog No Description Package Catalog No 10 ml x 1ml TALON Metal Affinity TALON Superflow Metal Affinity Resin 25 ml HisTALON Superflow Cartridge 1x 5ml ml x 5ml ml HisTALON Superflow Cartridge 20 Rxns (5 x 1 ml) ml Purification Kit 5 Rxns ( 5x 5 ml) ml HisTALON Gravity Column (1ml) 5 columns ml HisTALON Gravity Purification Kit 20 purifications 更多產品請上 網站查詢 January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

12 Millipore 樣品製備的好幫手 Amicon Ultra-2 Amicon Ultra 新成員 Dead volume stop :, Heat sealing :, sample : invert spin, :, :, buffer : 切向流技術 ( Tangential Flow Filtration,TFF ) 採用最新切向流技術取代傳統過濾法, 使回收率大幅提高! ( Normal Flow Filtration, NFF ),,, ( Tangential Flow Filtration, TFF ),,,,, 操作簡單 January - March Q1 簡易步驟 拿到 Sample 好簡單 Step 1 加樣品 Step 2 離心 Step 3 取樣品 透析真簡單, 10 台北 新竹 台中 嘉義 台南 高雄 服務專線

13 輕鬆去除 Detergent, Millipore Real Physical Dead Stop Volume 離心再久也不用擔心樣品離心離到乾 (0.5ml 亦可 invert spin) 濃縮後的樣品體積差異小, 方便不同管樣品後續實驗的濃度計算及體積調整 Detergent Millipore 大獲全勝 濃縮離心管領導品牌它牌無法比 High Speed + High recovery = Millipore Amicon Ultra Amicon Ultra 可以在較短的時間裡, 得到產量最高 濃縮倍率也最高的樣品 Round 1 不管在高濃度或低濃度的樣品中,Millipore 的 Amicon Ultra 皆可以給您比它牌更高的產率 :0.1mg/ml & 1mg/ml Aprotinin 勝 勝 :0.025 mg/ml & 0.25mg/ml cytochromec 勝勝 Nickel Magnetic Beads recombinant protein, Buffer Millipore Data from : user 勝 勝 January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

14 2011. January - March Q1 Millipore Proteins Choose a rotor and G force Concentration Factor Molecular Weight (MW) 選擇祕笈 Starting Volume <0.5 ml <2 ml <4 ml <15 ml Final Volume µl µl 50 µl 200 µl Design of the Device Standard 1.5 ml Standard 15 ml Standard 15 ml Standard 50 ml 5,000 g for 100k Fixed-Angle (35 º) 14,000 g 7,500 g 7,500 g for all other Rotor 1,000 g reverse spin 1,000 g reverse spin MWCO 5,000 g Swinging Bucket 4,000 g N/A Rotor 1,000 g reverse spin 4,000 g 4,000 g Concentration Factor X25 - X30 X14 X67 X80 X75 6<MW<20k 3,000 3,000 3,000 3,000 20<MW<60k 10,000 10,000 10,000 10,000 60<MW<100k 30,000 30,000 30,000 30, <MW<200k 50,000 50,000 50,000 50, k<MW 100, , , ,000 Single-Stranded and Double-Stranded Nucleic Acids Length Round 2 Round 3 Nanoparticles Particle Diameter (DIA) Millipore 可以給您比它牌更高的濃縮倍率 8X 46X 68X 221X 勝 1mg/ ml BSA, 30K, Millipore Millipore 可以在較短的時間內給您較高的產率 0.25 mg / ml cytochrome C,Millipore bp 30,000 30,000 30,000 30, min., fixed angle 10 min., 5,000 g, 10 min., 5,000 g, Time (30k) 10 min. 40 min., swinging fixed angle fixed angle rotor 1.5< dia< 3nm 3,000 3,000 3,000 3,000 3< dia< 5nm 10,000 10,000 10,000 10,000 5< dia< 7nm 30,000 30,000 30,000 30,000 7< dia< 10nm 50,000 50,000 50,000 50,000 10nm< dia 100, , , ,000 勝 12 台北 新竹 台中 嘉義 台南 高雄 服務專線

15 ZipTip Pipette Tips 微量樣品的純化選擇 ZipTip Millipore polymer chromatography beads Pipette Tip,, Mass HPLC, Millipore 30min 內快速完成實驗 ( Bind, Wash, Elute ) 可純化 Fetomoles 到 picomoles 的 proteins peptides 或 oligonucleotides 增加 Mass HPLC capillary electrophoresis 等方法的敏感度及 resolution 可耐 ph 1.5~ 13.5,pH 耐受範圍廣 : 增加實驗再現性 操作簡單 Equilibrate Ziptip pipette tip 使用 ZipTip 能有效去除鹽類的干擾 使用 ZipTip 能幫助富集 phosphopeptides Bind peptides/ proteins Wash away contaminants Elute purified peptides/ proteins MALDI MS Spectra of a Tryptic peptide digest from an ingel2d digest. The top spectra represents a contaminated sample prior to sample clean-up. The lower spectra represents the sample after using a ZipTipC18 prior to MALDI TOF MS analysis. MALDI TOF mass spectra of glycogen phosphorylase tryptic peptides (200 fmol) contaminated with Triton X-100 or glycerol (left). The spectra on the right are of the same samples that have been prepared using ZipTipSCX. 訂購資訊 resin 應用鍵結能力 Eluted volume 貨號品名包裝 ZTC18S008 ZipTip w/ 0.6µL C18 resin 8 pk C18 "Desalting/concentratng peptides and proteins Stepfractionation of complex peptide mixtures" 5ug 1~4ul Micro-bed of C18 2.0ug <1ul C4 strong cation metal chelate Desalting and concentrating proteins "Removing detergent Concentrating peptides from organic solutions" "Enriching phosphopeptides purification of His-tag proteins and peptides " 3.3ug 1~4ul 1~4ul ZTC18S024 ZipTip w/ 0.6µL C18 resin 24 pk ZTC18S096 ZipTip w/ 0.6µL C18 resin 96 pk ZTC18M008 ZipTip with 0.2 µl C18 resin 8 pk ZTC18M024 ZipTip with 0.2 µl C18 resin 24 pk ZTC18M096 ZipTip with 0.2 µl C18 resin 96 pk ZTC04S008 ZipTip with 0.6 µl C4 resin 8 pk ZTC04S024 ZipTip with 0.6 µl C4 resin 24 pk ZTC04S096 ZipTip with 0.6 µl C4 resin 96 pk ZTSCXS008 ZTSCXS024 ZTSCXS ng 1~4ul ZT0MCS008 ZipTip with 0.6 µl strong cation resin ZipTip with 0.6 µl strong cation resin ZipTip with 0.6 µl strong cation resin ZipTip with 0.6 µl metal chelate resin ZT0MCS024 ZipTip with 0.6 µl metal chelate resin ZT0MCS096 ZipTip with 0.6 µl metal chelate resin 8 pk 24 pk 96 pk 8 pk 824pk 896pk January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

16 ZYMO Signosis microrna 研究小幫手 ZR RNA MicroPrep and ZR RNA MiniPrep 產品規格 : Sample Sources : cells, solid tissues Sample Size : Microprep : 10 5 cells, ~10 mg tissues Miniprep : 10 7 cells, ~25 mg tissues Maximum RNA Binding Capacity : Microprep : 5 µg Miniprep : 25 µg Elution Volume : Microprep : 6 µl Miniprep : 25 µl RNA Purity : A260 / A A260 / A 萃取 回收小片段 RNA (trnas, micrornas) 也很 OK 喔! 回收 RNA 產量 品質遠勝 Q 牌! 全部搞定! 同時定量觀察 1) Z R 1 k b D N A M a r k e r ( C a t. #M5003, M5006) [agarose gel] 2-4) 2, 4, 9 µg total RNA (yeast) + ZR small-rna ladder mix [ agarose gel] 5) ZR small-rna ladder (17-29 bp ssrna oligos) [PAGE] 6-7) 300, 600 ng ZR small-rna ladder isolated with ZR RNA MicroPrep [PAGE] 快速 : 利用 Fast-Spin Column 迅速取得 RNA ( ~15 minutes ) 濃縮 :RNA 回收體積 6 µl, 回收產物適用於 RT- PCR 等 RNA-based 實驗便利 : 不需外加 organimc denatures β- mercaptoethanol proteinase K * = genomic (>10 kb) and plasmid (>3 kb) DNA contamination DNaseΙ= samples treated with DNaseΙ Signosis MicroRNA Northern Blot Assay Kit January - March Q1 操作安全 : 非放射性偵測提供一百多種合成好的 biotin-labeled mirna porbes All components in one kit: gel membrane RNA marker (20 & 60 nt) substrate 一應俱全操作流程簡單 訂購資訊 Cat. No. Product Size Zymo Total RNA Including mirna Extraction Kit R1060 ZR RNA Microprep 50 rxns R1061 ZR RNA Microprep 200 rxns R1064 ZR RNA Miniprep 50 rxns R1065 ZR RNA Miniprep 200 rxns Signosis mirna Northern Blot Assay Kit NB-0001 MiRNA Northern Blot Assay Kit Sample mirna extraction Electrophoresis mirna seperation by size Northern blotting (Transfer of mirna to membrane) mirna fixed to membrane with UV or heat MP-XXXX mirna probes 20 rxns 操作流程 Biotin-labeled mirna probe Membrane hybridized with labeled probes Visualization of labeled RNA on X-ray film For 2 mirna probes, each for monitoring 14 RNA samples; for 6 mirna probes, each for 2 samples 14 台北 新竹 台中 嘉義 台南 高雄 服務專線

17 Signosis MicroRNA Profiling 讓您一次觀察多種 mirnas : mirna Array & mirna Plates mirna mrna, 不用分離 mirna isoforms, ; 獨家 Oligo Logation Method, 可有效區別 isoform,signosis mirna isofoms Oligo Ligation Method (OLM), mirna 操作簡單 isoforms, membrane plate 靈敏度高達 femtogram ZYMO Signosis 獨家研發 mirna Array 技術原理 mirna DNA oligo, mirna/dna hybrid,dna ligase DNA oligo ;, DNA ligase mirna/dna stacking sequence,, ligated-dna T7 promoter, biotin transcript ; mirna array mirna 獨家研發 mirna Plate Assay 技術原理 capture probe (CP) mirna biotinlabeled detection oligo (DO) bridge DNA mirna bridge DNA, CP, DO, OLM mirna mirna Array 能有效區別僅一個核甘酸的差別 1fg mirna, OLM 不同癌細胞在 Cancer mirna Array 表現的差異 訂購資訊 Cat. No. Product Size MiRNA Number Membrane Assay AP-0001 Human mirna array I 3 membranes 60 AP-0002 Human mirna array II 3 membranes 72 AP-0003 Cancer mirna array 3 membranes 132 AP-0005 Mouse mirna array 3 membranes 119 AP-0006 Rat mirna array 3 membranes 113 Plate Assay MA-1001 mirna Direct Hybridization Plate Array I 2 assays 48 MA-1002 Apoptosis-Associated mirna Plate Array 2 assays 48 MA-1003 Stem Cell-Associated mirna Plate Array 2 assays January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

18 Clontech Mir-X MicroRNA Quantification mirna / cdna 定量試劑 1 一組試劑同時定量你的 target mrna 及 mirna 2 經濟實惠 all-in-one 型式, 令人驚艷的價格 3 高靈敏度低至 50 Copies 的 mirna 4 超簡單單管反應即可反轉您的 mirna 最經濟最方便 反轉一次, 即可進行多次 qpcr! Sense mirna Primer! 1 Polyadenylation 2 Annealing 3 Reverse transcriptase 3,! January - March Q1 省錢就是賺錢! mirna sequence 哪裡找? Sanger mirbase 為最大最完整的 mirna 資料庫 Clontech A 牌 Q 牌 RT 原理 Tailing RNA, Universal primer Stem-loop specific primer Tailing RNA, Universal primer Genes/ RT Various ( including cdna mirna) Only mirna Various ( including cdna mirna) RNA maximum loading 8 ug 10 ng 1 ug qpcr reactions/ RT Cost / RT $ $$$$$ $$$ 16 台北 新竹 台中 嘉義 台南 高雄 服務專線

19 經濟實惠的價格, 卻不失專一性! Clontech Specific quantification of Let7 mirna variants. Using mirna-specific primers (Panel A), Mir-X qrt-pcr was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast polya+ RNA (Panel B). The primers detected each of their corresponding Let7 mirna cognates, but did not detect the offtarget variants in 63 of 64 possible combinations. Mouse P19 細胞的經 Retinoicacid 處理後,miR-9 表現上升 Trichostatin A treatment alters mirna expression in mouse ES cells. Mouse embryonic stem cells were harvested either prior to or after being treated with trichostatin A (TSA) for 18 hr. RNA was prepared from the cells, and was then analyzed by Mir-X mirna qrt-pcr using primers specific for the 12 indicated mirnas and for a p21 control mrna known to be induced by TSA. ES 細胞在 Trichstatin A 處理後 mirna 的變化 Induction of mir-9 in mouse P19 cells. P19 mouse embryonal carcinoma cells were plated on agarosecoated petri dishes and allowed to form embryoid bodies (EB) in the presence or absence of retinoic acid (RA). After five days of culture, EBs were dissociated with trypsin and replated on tissue culture dishes without RA. Cells were harvested on days 3 7 and 10 13, and the induction of mir-9 was followed and quantified using the Mir-X mirna qrt-pcr SYBR Kit and primers specific for mir-9 and U6 (as a normalization control). 訂購資訊 單筆滿 20, Mir-X 折 再送 (TAK #9037) RNase-OFF Mir-X mirna First-Strand Synthesis Kit 20 rxns 10, Mir-X mirna qrt-pcr SYBR Kit 200 rxns 16, Mir-X mirna First-Strand Synthesis Kit 60 rxns 15, Mir-X mirna qrt-pcr SYBR Kit 600 rxns 31, January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

20 2011. January - March Q1 SBI microrna Overexpression & Inhibition Research SBI microrna overexpression / inhibition lentivectors Lentivirus, / microrna Lentivectors transient transfection SBI microrna, Sanger's mirbase ( microrna, antisense microrna, microrna, 持續表現 micrornas (Pre-cloned) Cat # PMIRHxxx-AA-1 Cat # PMIRHxxx-AA-1 native mirna, processing function transient transfection lentivirus infection GFP gene, cell sorting stable clones lenti-mir,! 持續抑制 endogenous microrna 表現 (Pre-cloned) Cat # MZIPxxx-AA-1 anti-sense mirna mirna, mirna transient transfection lentivirus infection GFP gene puromycin-resistant gene, stable clones mirzip Cat # MZIPxxx-AA-1 實例一 Suppression of cell invasion and tumor metastasis by mirzip-21. cancer mirna mir-21, tumor invasion 實例二 Western blot probed with α-target protein antibodies 18 台北 新竹 台中 嘉義 台南 高雄 服務專線

21 特別介紹 MicroRNA Target Selection System Interaction between mirna and mrna 3'UTR 研究 mirna 好幫手! SBI 產品特點 Dual sensor 3'UTR lentivector system: luciferase (Fire) cytotoxic sensor (Ctx) mirna mrna 3'UTR 獨特設計 : Ctx mirna & 3'UTR binding 結果數量化 : luciferase signal 一體兩用 :, lentivirus 秘密武器 : puromycin resistant gene, stable cell lines; 3'UTRs in genome-wide mir screens 由 mirna & 3'UTR binding 決定 1 細胞存活率 2 luciferase 表現量 Case No. 1:No mir Binding Ctx, Ctx drug luciferase, Case No. 2:Successful mir Binding Ctx, Ctx drug luciferase, 利用 mir-selection system 觀察 mir-145 與 c-myc 3'UTR 結合情形 C 訂購資訊 (A) The cytotoxic screen was performed using control (no 3 UTR) and c-myc 3 UTR mir-selection lentivectors. Both stable cell lines were transduced with equal amounts or Lenti-miR-145 virus (GFP marker). The cells were treated for 4 days with 1x Ctx cytotoxin drug. (B) The Ctx sensor is down regulated by mir-145 binding to c-myc 3 UTR which enables survival in Ctx screens and also (C) reduces luciferase activity by 63%. Cat No Product Size MS040A-1 Ctx drig, 1000 X 500 µl MS410A-1 MicroRNA Target Selection Kit (mir- Selection lentivector + Ctx drug) 10 µg January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

22 Lonza 最夯的細胞 ~ HUVEC ( primary cells ), (Non-immortalized), ( cancer cells ) ( cell line ), ( In vivo ), ( coagulation ) ( fibrinolysis ),, ( Human Umbilical Vein Endothelial Cells; HUVEC), LONZA, Primary cells,, Primary Cells 5 ~ 10 Donor QC 1. CD31/CD105 90% double positive 2. Positive - acetylated LDL uptake and Factor VIII 3. Negative - smooth muscle α-actin (α-sma) ISO9001:2001, Cat No. C2517A Tube formation CD31/CD105 douple positive January - March Q1 訂購資訊 HUVEC 最適化的培養液 EGM-2 Bulletkit Cat. No. Description Package HUVEC-Umbilical Vein Endo, Pooled 1x10 7 cells/vial C2517A HUVEC-Umbilical Vein Endo in EGM-2, Single Donor > 5x10 5 cells/vial C2519A HUVEC-Umbil Vein, Pooled in EGM-2, Pooled > 5x10 5 cells/vial CC-3162 EGM -2 Endothelial Cell Growth Medium-2 Bulletkit ( CC CC-4176) Hot Kit Basal Medium Supplements, HUVEC 15 PD ( population doublings ) HUVEC C2519A + 合購價 14,000 元 EGM-2 Bulletkit 500 ml 20 台北 新竹 台中 嘉義 台南 高雄 服務專線

23 細胞培養 Q1 A Q2 A Q3 A Q4 A 如何解凍 Primary cells? Effective Growth Area Q&A, 37,,,, Pipetting,, 37 PS:! primary cells 如何使用細胞培養液 Bulletkit? Supplements, Supplement Growth factor Basal Medium,, PS: Supplement Growth factor,, Bulletkit 的培養液是否含有抗生素? 需要額外添加嗎? Bulletkit 30 ug/ml Gentamicin 15 ng/ml Amphotericin-B, KGM-CD, 如何挑選適當的培養器皿?,,, Cell Culture Medium Required Initial Number of Cells to Seed at 2,500 cells/cm 2 Initial Number of Cells to Seed at 3,500 cells/cm 2 Initial Number of Cells to Seed at 5,000 cells/cm 2 Lonza T cm 2 5 ml 62,500 87, ,000 T cm 2 15 ml 187, , ,000 T cm 2 30 ml 375, , , mm 9.6 cm 2 2 ml 20,000 28,000 40, mm 21 cm 2 5 ml 52,500 73, , mm 55 cm 2 11 ml 137, , , mm 148 cm 2 30 ml 370, , ,000 Q5 A Q6 A 我需要在培養 Primary cells 的培養皿底部,coating Gelatin, Fibronectin, Collagen 或 Matrigel 嗎? Clonetics ( LONZA ) coating ECM ( Extracellular Matrix ) ( Hepatocytes ) ( bovine brain microvascular endothelial cells ) ( rat/mouse neuronal ) ( dorsal root ganglion neurons ) ( rat cardiac myocytes ) coating ECM,, :Chamber Slider, coating ECM Primary cells 是否可以培養大量保存? Primary cells,, 1ml 2 ml 5x x10 6 /ml, -80 Overnight, Primary cells,,,, January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

24 2011. January - March Q1 Millipore 溝通橋樑 ~Signaling transduction,,,,, ( receptor ), ( ligand ), ( Signaling transduction ),,, :1. 2. ( Secondary Messengers ) 3. Millipore, 神經訊號 ;,, ;,, VEGF-Integhn Blnding Pathway Cytokine Induced Pathway EGF Cat No EGFR Cat No SMAD-2 Cat No EGF staining on colon cancer tissue via anti-egf( ) and the Millipore IHC Select HRP-DAB detection system. 訂購資訊 EGFR (red) expression in A431 cells is demonstrated using a Cy-3 conjugated primary antibody ( ) via confocal immunocytochemistry. Nuclear staining (blue) and actin staining (green) was achieved through use of DAPI and Alexa Fluor 488 conjugated phalloidin, respectively. IGF expression pattern and morphology on ductal breast carcinoma revealed using anti-igf ( ) and Millipore IHC Select detection with HRP-DAB. Cat No. Item Description Size Cat No. Item Description Size Anti-EGF 100 ul Anti-JAK2 150 µl Anti-EGFR (cytoplasmic domain) 100 µg GF003 FGF-2 (bfgf), recombinant human 50 µg Anti- FGF2 BFGF, Clone BFM2 500 µg GF144 EGF, recombinant human 500 µg Anti-IGF-1, Clone SM µg GF138 IGF-1, recombinant human 100 µg Anti-FGFR µg GF094 VEGF, recombinant human 10 µg Anti-IGF µg PDGFRa, active 50 µg Anti-EGFR, polyclonal 200 µg GF023 Tumor Necrosis Factor-a, recombinant human 50 µg Anti-SMAD2 100 µl IL004 Interleukin 4 (IL-4), recombinant human 10 µg Anti-TNF 100 µl IL006 Interleukin 6 (IL-6), recombinant human 20 µg 22 台北 新竹 台中 嘉義 台南 高雄 服務專線

25 次級訊號傳遞因子 (Secondary Messangers), ( Secondary Messengers ) ( Primary effectors) : ( Ion channels ),G ( G proteincoupled receptor ), ( catalytic receptor ), ( responses ), Millipore camp HTS Immunoassay kit Cat No 靈敏度高安全 : 可見光方式偵測操作簡單定量 : 含標準品, 方便定量 All in one: 包含實驗所需物質 Assay Overview 訂購資訊 Cat No. Item Description Size Cat No. Item Description Size Anti-Calcineurin 100 µl Anti-phospho-IRS1 (Ser307) 100 µg Anti-RAC1, clone 23A8 250 µg Anti-IRS1, Alexa Fluor 555 conjugate 50 µg camp HTS Immunoassay Kit (Chemiluminescent) 960 assays Anti-IRS1 100 µg camp HTS Immunoassay Kit (Colorimetric) 192 assays MILLIPLEX map 10-Plex MAPK/SAPK Signaling Kit 100 assays camp HTS Immunoassay Kit (Colorimetric) 960 assays MILLIPLEX map 8-Plex Multi-Pathway Signaling Kit 100 assays Anti-GRK 2/3 (bark 1/2) 100 µg MILLIPLEX map Total CREB MAPmate kit AB5674P Anti-Acid Sensitive Ion Channel 1 50 µg MILLIPLEX map Total Erk/MAP Kinase 1/2 MAPmate kit AB5156 Anti-Calcium Channel, Voltage Gated a 1C 200 µl MILLIPLEX map Total IRS1 MAPmate kit Anti-IRS1, Alexa Fluor 488 conjugated 50 µg Erk 1/2 STAR ELISA Kit 96 assays Anti-phospho-TIE2/TEK (Tyr992) STAR FAK ELISA Kit 96 assays Anti-MAPK 1/2 100 µg "Erk 1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Dual Detect CELISA Assay Kit (Fluorogenic Detection)" kit Anti-Met (extracellular), clone 4F µg TrkA, active 10 µg Anti-phospo-c-Abl(Tyr 245) 100 µl Src, active 10 µg 磷酸化蛋白的調控 The Millipore camp HTS immunoassay demonstrated superior results versus a non-millipore (Brand T) camp detection system in CHO cells pretreated with isobutylmethylxanthine phosphodiesterase inhibitor and forskolin. 訂購資訊 4G10 Cat No FOXO1 Cat No Confocal fluorescent analysis of A431 and HeLa cells using anti-foxo1 ( ) ( r e d ), A c t i n (green) and nucleus (blue). Ras Cat No Immunohistochemistry staining of stratified human squamous epithelium using anti-ras ( ) and the Millipore IHC Select Detection System. Cat No. Item Description Size Cat No. Item Description Size G10 Platinum, Anti-Phosphotyrosine 100 µl Ras Activation ELISA Assay Kit Kit AMPK (a1, b1, g1), active 10 µg Rac1/Cdc42 Activation Assay Kit 30 assays Anti-Sulfotyrosine, clone Sulfo-1C-A2 100 µl Anti-PI3 Kinase, p110b 100 µl Anti-DEPTOR 100 µg Anti-Inositol Hexakisphosphate Kinase Anti-mouse Tks4 100 µg Anti-Phospholipase A2 100µg Anti-Ubiquitin, Lys48-Specific, clone Apu2 100 µl Anti-Acetyl-Lysine 100µg Anti-phosphoserine, clone 4A4 100 µg Anti-dimethyl-Arginine, asymmetric (ASYM24) 200 µl MAB374 Anti-Glyceraldehyde-3 PDH 200 µg Anti-phospho-ATM (Ser1981) 200 µg MAB1510 Anti-Ubiquitin 100 µl Anti-CBP NT 200 µg Anti-NIitrotyrosine 100 µg Anti-ATR 100 µg Anti-Ubiquitin, Lys63 specific 100 µl Anti-FOXO1 200 µg mtor (1362-end), active 10 µg Anti-TCF-4, clone 6H µg MAB5414 Anti-NOTCH1 100 µl Anti-TCF-3/4, clone 6F µg Anti-Ras Assays & Kits 100µg Anti-TCF-1, CL 7H3 PC µg 台北 新竹 台中 嘉義 台南 高雄 服務專線 January - March Q1 23

26 BD 輕鬆測得抗原專一性 T 細胞免疫反應 T 3 H-thymidine 試驗方法二 incorporation T 51 Cr release assays T,,Enzyme-linked immunospot (ELISPOT) assay 試驗方法三 Intracellular cytokine assay MHC-peptide staining T Isotope 試驗方法一 T, T T ELISPOT Intracellular cytokine assays T Cytokine, MHC-peptide staining Peptide T, Cytokine Cytometric Bead Array T BD,, 試驗方法一 ELISPOT 免疫斑點分析 靈敏度最高 January - March Q1 ELISPOT 原理 ELISPOT ELISA, Enzyme-linked Immunospot Assay BD ELISPOT NA / LE (No sodium azide Low endotoxin) PVDF 96,,, Biotin, Avidin-HRP, AEC, 10-20µm, (ELISPOT Plate Reader),,, 應用 Th0/Th1/Th2 購買 Antibody Pair 可以半價加購 Detection of IFN-γ-secreting cells by BD ELISPOT Human IFN-γ Kit (Cat. No ). Human PBMCs were stimulated with PMA and ionomycin for 18 hours. (blank controls: G 1-9: cells without stimuli; H 1-9: stimuli without cells) 特點 最靈敏 - 可偵測到 30 萬分之一正在分泌 cytokine 的細胞 不挑剔 - 新鮮或解凍的細胞都可以 最新鮮 - 相較於 ELISA, ELISPOT 偵測剛剛從細胞正要分泌出來的細胞激素 很環保 - 刺激過後的細胞還可以再利用 購買 Set 贈送 一組 購買 Kit 優惠價 9 折 特惠活動至 3 月 31 日止 24 台北 新竹 台中 嘉義 台南 高雄 服務專線

27 1 Capture Antibody For Sets and Pairs: Coat 2 Blocking Block unoccupied sites 3 Add Cells Incubate cells in well with 4 Wash Cells are washed off BD microwells with with protein Ag/stimulus etc. anti-cytokine capture antibody. For Kits: Go to Step 3; Steps 1 and 2 not necessary. 5 Detection Antibody 6 Enzyme-Avidin 7 Develop With Substrate Add Biotinylated anti-cytokine Add Avidin-HRP Add substrate and detection antibody monitor formation of colored spots BD 的好比較就知道!! 他牌 BD ELISPOT 可以得到清晰的點關鍵就是 Capture antibody 嚴選高專一性 NA / LE 無毒抗體 (no azide / low endotoxin format : sodium azide endotoxin) Panel A Panel B BD Biosciences Pharmingen ELISPOT (NA / LE), PBMC,, (Panel A) ELISPOT 包裝 Component Antibody Pair Set Kit BD ELISPOT plates 10 uncoated 2 Antibody-coated and blocked Capture antibody For 5 plates For 10 plates Pre-coated on plates Detector antibody (biotin-conjugated) For 5 plates For 10 plates For 2 plates Streptavidin-HRP, : For 10 plates For 2 plates AEC chromogen, : 需另外購買, 貨號 : For 2 plates Wash concentrate, PBS concentrate, assay diluent For 2 plates * ELISPOT plates 建議搭配 Millipore MSIPS4510(MultiScreenHTS-IP Filter Plate)for ELISPOT Assay 訂購資訊 Description Size Cat. No. Description Size Cat. No. Human IL-2 ELISPOT Pair 5 plates Mouse IL-2 ELISPOT Pair 5 plates Human IL-2 ELISPOT Set 10 plates Mouse IL-2 ELISPOT Set 10 plates Human IL-4 ELISPOT Pair 5 plates Mouse IL-4 ELISPOT Pair 5 plates Human IL-4 ELISPOT Set 10 plates Mouse IL-4 ELISPOT Set 10 plates Human IL-5 ELISPOT Pair 5 plates Mouse IL-5 ELISPOT Pair 5 plates Human IL-5 ELISPOT Set 10 plates Mouse IL-5 ELISPOT Set 10 plates Human IL-10 ELISPOT Pair 5 plates Mouse IL-6 ELISPOT Set 10 plates Human IL-10 ELISPOT Set 10 plates Mouse IL-10 ELISPOT Pair 5 plates Human IL-12p70 ELISPOT Set 10 plates Mouse IFN-γ ELISPOT Pair 5 plates Human IFN-γ ELISPOT Pair 5 plates Mouse IFN-γ ELISPOT Set 10 plates Human IFN-γ ELISPOT Set 10 plates Mouse IFN-γ ELISPOT Kit 2 plates Human IFN-γ ELISPOT Kit 2 plates Mouse TNF ELISPOT Pair 5 plates Human TNF ELISPOT Pair 5 plates Mouse TNF ELISPOT Set 10 plates Human TNF ELISPOT Set 10 plates Rat IFN-γ ELISPOT Set 10 plates January - March Q1 Human Granzyme B ELISPOT Set 10 plates 台北 新竹 台中 嘉義 台南 高雄 服務專線

28 2011. January - March Q1 BD 試驗方法二 輕鬆測得抗原專一性 T 細胞免疫反應 Recombinant Soluble Dimeric Proteins (MHC-Ig 雙聚體重組蛋白 ) BD DimerX MHC, DNA MHC class I (α1, α2, α3 ) mouse IgG1 Variable Heavy (VH) N DNA β2- microglobulin DNA ( lambda ) ( myeloma cell line ), MHC-Ig β2-microglobulin DimerX MHC T 操作簡單 靈活搭配 彈性運用 偵測 cytomegalovirus (CMV) 病人淋巴球的專一性免疫反應 BD DimerX HLA-A2:Ig HLA-A2 CMV-pp65 peptide (NLVPMVATV), PBMC, anti-mouse IgG1-PE (Cat. No ), peptide ; peptide (NLVPMVATV) BD DImerX 優點 peptide DimerX, peptide-loaded DimerX T, T : CD44, CD62L, CCR7 Cytokine, T T ( : DimerX isotype mouse IgG1 Isotype mouse IgG1 ) DimerX peptide, T, T (T-cell epitope mapping) 結構穩定 DimerX, 專一性高 訂購資訊 DimerX I 不需固定 打洞 T Cell Cytokine ELISPOT,BD dimerx TCR MHC-peptide 偵測小鼠專一性 T 細胞免疫反應 BD DimerX I Mouse H-2Ld:Ig peptide QL9 (QLSPFPFDL; ) peptide MCMV (YPHFMPTNL; ) C T, anti-mouse IgG1-PE (Cat. No ), T DESCRIPTION REACTIVITY ISOTYPE SIZE CAT. NO. BD DimerX I Soluble Dimeric Human HLA-A2:Ig Fusion Protein Hu Ms IgG1, λ 0.05mg BD DimerX I Soluble Dimeric Human CD1d:lg Fusion Protein Hu Ms IgG1, λ 0.1mg BD DimerX I Soluble Dimeric Mouse H-2Kb:Ig Fusion Protein Ms Ms IgG1, λ 0.25mg BD DimerX I Soluble Dimeric Mouse H-2Ld:Ig Fusion Protein Ms Ms IgG1, λ 0.25mg BD DimerX I Soluble Dimeric Mouse H-2Db:Ig Fusion Protein Ms Ms IgG1, λ 0.25mg BD DimerX I Soluble Dimeric Mouse CD1d:lg Fusion Protein Ms Ms IgG1, λ 0.25mg Anti-Mouse IgG1 Ms Rat IgG2a, λ 0.1mg Human β2 Microglobulin Hu 0.1mg 台北 新竹 台中 嘉義 台南 高雄 服務專線

29 試驗方法三 In Vivo Capture Cytokine Assays 解救實驗老鼠 細胞激素體內偵測試劑 Cytokine,, : IL-4,, ELISA ELISA CBA,,,!, Finkelman Morris,, Cytokine / anti-cytokine mab complex ELISA,, Cincinnati cytokine capture assay ( CCCA ),BD In vivo capture cytokine assays CCCA BD NA / LE Biotin anti- IFN-γ mab Biotin anti- IFN-γ mab + IFN-γ T cell IFN-γ / Biotin anti- IFN-γ mab complex IFN-γ / Biotin anti- IFN-γ mab complex TMB Streptavidin-HRP IFN-γ/ Biotin anti-ifn-γ mab complex Measurement of In Vivo IL-4 and IFN-γ production by IVC. BALB/c mice (5/group) were injected i.v. with 10 µg each of biotinanti-il-4 mab (Cat. No ) and biotin-anti-ifn-γ mab (Cat. No ) ± 10 µg of anti-cd3 mab (Cat. No ). Mice were bled 4 hours later and serum levels of cytokine-biotin-anticytokine mab complexes were determined by ELISA. Purified anti-ifn-γ mab In vivo capture cytokine assays 優點 高靈敏度 ELISA 體內監測,, 操作簡單 10µg NA/LE Biotin anti- IFN-γ mab 2-72 ELISA 動態觀察 50µl,, 訂購資訊 Description Size Cat. No. IFN-γ, Mouse In Vivo Capture Assay 10 plates TNF, Mouse In Vivo Capture Assay 10 plates IL-10, Mouse In Vivo Capture Assay 10 plates IL-6, Mouse In Vivo Capture Assay 10 plates IL-4, Mouse In Vivo Capture Assay 10 plates IL-2, Mouse In Vivo Capture Assay 10 plates Assay Components : In Vivo Capture monoclonal antibody (biotinylated, BD NA/LE ) Streptavidin-HRP Recombinant cytokine standard Purified capture monoclonal antibody for plate coating January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

30 2011. January - March Q1 BD CBA Enhanced Sensitivity Flex Set System NEW 新品上市 史上最強 Femtogram 等級的 Cytokine 偵測試劑 Enhanced Sensitivity 靈敏度最強,BD Cytometry Beads Array (CBA) ;,BD BD CBA Enhanced Sensitivity Flex Set System, 5-10 pg/ml,bd CBA Enhanced Sensitivity Flex Set System 274 fg/ml, 35,,,, BD CBA Enhanced Sensitivity Flex Set System CBA Enhanced Sensitivity Flex Set 原理 1. 定量範圍 274 to 200,000 fg/ml 2. 多參數分析, 單一檢體可得到多種定量結果 3. 彈性組合 任意搭配完全符合實驗需求 4. 最多可同時分析 10 種 Cytokine, 只需 25~50 µl 檢體量 5. 適用任何搭載 488nm 和 633nm 雷射光源的流式細胞儀 一定要親自體驗 BD CBA Enhanced Sensitivity Flex Set CBA, Cytokine, (Capture antibody),, PE (Detection antibody), PE,, BD Detection Reagent (Part A) Enhanced Sensitivity Detection Reagent (Part B) Cytokine,, Detectiontion reagent Part A + Part B CBA Flex Set 升級版 28 台北 新竹 台中 嘉義 台南 高雄 服務專線

31 Time course of human PBMCs stimulated with PMA and Calcium Ionophore. BD Enhanced Sensitivity CBA Flex Set CBA Flex Set ELISA 靈敏度 274 fg/ml 5-10 pg/ml CBA 單次分析因子數, 10, 30 1 反應作用時間 需清洗步驟次數 檢體偵測重複 檢體量 25-50µl 25-50µl 100µl 檢體分析形式 分析儀器 訂購資訊 Description SIZE Cat. No. Human IFN-γ Enhanced Sensitivity Flex Set (Bead B8) 100 Tests Human IL-1β Enhanced Sensitivity Flex Set (Bead B4) 100 Tests Human IL-2 Enhanced Sensitivity Flex Set (Bead A4) 100 Tests Human IL-4 Enhanced Sensitivity Flex Set (Bead A5) 100 Tests Human IL-5 Enhanced Sensitivity Flex Set (Bead A6) 100 Tests Human IL-6 Enhanced Sensitivity Flex Set (Bead A7) 100 Tests Human IL-8 Enhanced Sensitivity Flex Set (Bead A9) 100 Tests Human IL-10 Enhanced Sensitivity Flex Set (Bead B7) 100 Tests Human IL-12 p70 Enhanced Sensitivity Flex Set (Bead C6) 100 Tests Human TNF Enhanced Sensitivity Flex Set (Bead C4) 100 Tests Human Enhanced Sensitivity Master Buffer Kit 100 Tests Human Enhanced Sensitivity Master Buffer Kit 500 Tests January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

32 BD CycleTEST Plus DNA Kit 細胞週期分析套組 BD CycleTEST TM PLUS DNA, (Cell Cycle) DNA (DNA ploidy analysis), DNA ( ),, DNA, G2/M G0/G1, S 1. Sample 收集處理後, 可暫時冷凍 (-80 ), 待日後檢測 2. 可應用於多種類的細胞懸浮液及組織之 Cell Cycle 及 DNA 指數 (DNA Index) 分析 實驗原理,, (-80 ),,,, PI PI nm, FL2 分析方法 ( ) DNA : (PBMC) reference, DNA DNA 指數 (DNA index) = 異常倍體 G0/G1 位相平均螢光值 (FL2-A) / 正常細胞 G0/G1 位相平均螢光值 (FL2-A) January - March Q1 FL2-A Counts full scale FL2-A Counts full scale 訂購資訊 Fig 1. (PBMC) : gating ( ) Cat.# Name Contents Apps Size Status FL2-W BD Cycletest Plus DNA Reagent Kit FL2-A FL2-W FL2-A Fig 2. (PBMC) : Fig 1 gating Fig 3. : gating ( ) Solution A: trypsin in spermine tetrahydrochloride detergent buffer; Solution B: RNase A and trypsin inhibitor in spermine buffer; Solution C: Propidium Iodide (PI) in spermine buffer; Buffer Solution: dimethylsulfoxide (DMSO) in sucrose-sodium citrate Fig 4. : Fig 3 gating FCM 40 tests RUO (GMP) PI/RNase Staining Buffer FCM 100 ml RUO 30 台北 新竹 台中 嘉義 台南 高雄 服務專線

33 蛋白質電泳系統 The Most trusted Name in Electrophoresis Since 1967 蛋白質分析基礎工具系列 Hoefer SE260 10x10cm x 2 gels SE600 18x16cm x 2-4 gels SE640 18x8cm x 2-4 gels IEF IPG strips 蛋白質轉印系統 電源供應器 Semi-Dry unit 14x16 or 21x26cm TE62 15x21cm x 4 blots TE2 9x10cm x 4 blots 300V/500mA or 200V/2000mA 蛋白質樣品製備系統 ~ 透析系列 DIALYZER Ultra-Fast DIALYZER SpinDIALYZER Fast SpinDIALYZER 蛋白質樣品前處理與純化 TipColumn SpinColumn 蛋白質樣品前處理 樣品濃縮與純化 適用於一般的 Pipette 快速且回收率高 樣品體積 10 25µl & 25 75µl 樣品體積 10 ~ 25µl, 25 ~ 75µl & 75 ~ 150µl 規格有單支與 96 孔盤式 簡單好操作 Teflon 材質, 低殘留 可重複使用, 可 autoclave 完全密封不滲漏 Ultra Fast DIALYZER TM 雙側開口設計, 加速透析 SpinDIAlYZER TM 內含磁性體, 可直接放置在磁攪拌器上 樣品體積 DIALYZER 10µl 5ml SpinDIALYZER 10µl 1.5ml 可供選擇的介質種類 TipColumn SpinColumn Gel Hydrophobic Hydrophilic Ion Filtratio Misc. (non-polar) (polar) Exchange n C18 Sillica G-10 Strong Anion Activated Charcoal Cyano C8 G-25 Weak Anion (CN) Cellulose Amino Strong C4 G-50 Detergent (NH 2) Cation Removal Hydrophili G-100 Weak Cation c IMAC P-2 P January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

34 2011. January - March Q1 Cell Biosciences FluorChem Family Cell Biosciences 提供高品質及高效能的完整影像系統產品線, 讓您 實驗應用及需求選擇適合的產品, 達到實驗的 的 您有特殊需求, 也提供產品 製化的服務 Applications include but not limit to... Fluorescent dyes SYBR Green SYPRO Ruby Cy3 and Cy5 Detection GFP Multicolor Fluorescent Westerns EtBr Stained Gel Chemiluminescent samples White Light Applications Protein Array Western Blots 2D Protein Gel Silver Stain DotBlot Microtiter Plate Colony Plate Coomassie Blue FluorChem Family 產品線 型號 FC2 HD2 Q E 特點 CCD 畫素 CCD Bit Depth 16 bit 降溫 -25 o C Absolute and regulated 升級性 2D 2D 32 台北 新竹 台中 嘉義 台南 高雄 服務專線

35 Pearl Impulse Imager LI-CDR 近紅外光螢光動態活體影像系統 5S Sensitivity Simplicity Size Speed Savings 市面上最靈敏的高解析度螢光影像系統市面上操作最容易的螢光影像系統市面上最小的螢光影像系統市面上拍攝速度最快的螢光影像系統市面上最經濟的螢光影像系統 Fast High Resolution Dynamic Imaging 快速建立您的活體動物實驗 Biodistribution Tissue / Organ imaging Detection of native tumors, Cell tracking, Targeted therapeutics Vascularization Bone imaging Lymphatic imaging Ready-to-use Optical Agents IRDye 800CW EGF IRDye 800CW 2-DG IRDye 800CW RGD IRDye 800CW PEG IRDye 800CW HA IRDye BoneTag TM CellVue Stains IRDye 800CW RGD IRDye 800CW EGF(Green) IRDye 800CW 2DG CellVue NIR815 cell stain IRDye 680 BoneTag TM (Red) IRDye 800CW PEG IRDye 800CW PEG IRDye 680 Bone Tag IRDye 800CW HA January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

36 Clontech Cell Fate & Cell cycle NEW Fucci cell cycle monitor, :G1 ( gap 1 ) S (DNA Synthesis ) G2 ( gap 2 ) M ( mitosis ) phase, G1/S G2/M M phase Checkpoint, ( G0 phase) Checkpoint,, 不需固定讓細胞週期立即現形 Fucci Probe, Cdt1 Geminin : G1 phase, Cdt1, S phase, Cdt1 poly-ubiqutination, Cdt1 Proteasome S phase, Geminin, Geminin M phase M phase Geminin Cdt1 Geminin, Fucci ( Fluorescent ubiquitination-based, cell-cycle indicators ) Probes Features of Clonetech Fucci Probes, Fucci Probe, / ptre-cellcycle Fucci Probe, Tet inducible system Visualize cell cycle with Fucci Probes - 以 ptre-cellcycle 為例 Cat 以紅光標記 G1 phase 藍光標記 G2SM phase 為例 : January - March Q1 G1 phase: G2S phase: M phase: Late M phase: G1/S Checkpoint: Cell cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the ptre-cellcycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: Cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mcherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1. 34 台北 新竹 台中 嘉義 台南 高雄 服務專線

37 Various Applications of Fucci Probes / RNAi / ( ) Cell model / Transgenic mice line Wound healing,,,, / ( Co-imaging ) Clontech 活用範例 Wound healing ( ) ( ) Wound healing assay, Fucci cell line, ( ) ( ) 你分的清楚紅色圈選的細胞是增生或是移動的結果嗎? 其他 Retroviral vectors 及表現特色 Cat pretrox-sg2m-cyan 表現特色 : S G2 phase: M phase: G1 Late M phase: Cat pretrox-g1-red 表現特色 : G1 phase: Cat pretrox-sg2mcyto-red 表現特色 : S G2 M phase: G1 Late M phase: Cat pretrox-sg2m-red 表現特色 : S G2 phase: M phase: G1 Late M phase: January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

38 NEPTUNE 品質最優的 Tip! 新春全面特價 優惠 1 Filter tip 買 4 送 1 優惠 2 其他產品滿額再送贈品 ( 非 Filter Tip) Tip 採用獨家特殊 Super Slick Surface(S3) 低殘留技術, 讓您珍貴的樣品一點都不浪費 Neptune 為美國 CDC 檢測 H1N1 SOP 的專用耗材 獨特儲存方式, 方便不佔空間 Neptune BT Barrier Tips No detectable DNA contamination No detectable PCR inhibitors No detectable deoxyribonuclease or ribonuclease No detectable pyrogen Pre-sterilized BT1000 BT10 XL January - March Q1 Neptune PN Description Qty/Pack ( ) BT10XL 10ul Extended Length Barrier Tip 10 Trays of 96 Tips 1,760 BT20 20ul Barrier Tip 10 Trays of 96 Tips 1,760 BT ul Barrier Tip 10 Trays of 96 Tips 1,760 BT ul Barrier Tip 10 Trays of 96 Tips 1,760 BT ul Barrier Tip 8 Trays of 60 Tips 1, ul Extended Length Tip 1000 Tips/bag ul Round Universal Fit Gel Tip 1000 Tips/bag ml Flat Cap PCR Tubes, Clear 1000 tubes/bag ml PCR 8 Strip Tubes with Strip Caps, Clear 125 strips/pack 2, ml Thin Walled PCR Tubes, Clear 1000 tubes/bag ml Clear, Graduated Microcentrifuge Tube 1000 tubes/bag ml Clear, Graduated Microcentrifuge Tube 500 Tubes/bag ml Clear, Graduated Microcentrifuge Tube 500 tubes/bag 360 SR S 50ml Reagent Reservoir, Pre-sterilized 5 Pc/Bag 120 BT20 36 台北 新竹 台中 嘉義 台南 高雄 服務專線

39 NEW PROSPEC 超過 19 年生產 Recombinant Protein 的經驗 Cytokines Growth Factors Chemokines Hormones Enzymes Protein kinases Recombinant & Natural Proteins Viral Antigens Monoclonal & Polyclonal Antibodies 熱賣商品 Cat No. Item Description Size CYT- 280 Recombinant Human Adiponectin 25µg CYT- 207 Recombinant Human Brain-Derived Neurotrophic Factor 10µg CYT- 560 Bovine Fibroblast Growth Factor-basic 5µg ANT- 312 Mouse Anti Human Bone Morphogenetic Protein-7 20µg ANT- 169 Mouse Anti Human Epidermal Growth Factor 1mg CYT- 232 Recombinant Human Follistatin 20µg ANT- 129 Mouse Anti Human Granulocyte Macrophage-Colony Stimulating Factor 1mg CYT- 216 Recombinant Human Insulin Like Growth Factor-1 100µg CYT- 228 Recombinant Human Leptin 1mg CYT- 418 Recombinant Human Myostatin 10µg CYT- 501 Recombinant Human Platelet Derived Growth Factor-BB 10µg CYT- 267 Recombinant Human Prolactin 50µg CYT- 334 Recombinant Human Soluble RANK Ligand 10µg CYT- 255 Recombinant Human Stem Cell Factor 10µg CYT- 368 Recombinant Human Transforming Growth Factor-Beta 3 10µg CYT- 223 Recombinant Human Tumor Necrosis Factor-alpha 50µg CYT- 241 Recombinant Human Vascular Endothelial Growth Factor 10µg January - March Q1 台北 新竹 台中 嘉義 台南 高雄 服務專線

40 流式細胞儀的領導品牌最高品質的抗體盡在 Pharmingen Pharmingen 特惠方案 方案一買就送 磁珠抗體細胞分離系統 IMag Cell Separation System 輕輕鬆鬆分離特定細胞 ~ ~ 買三支磁珠抗體就送磁座或磁珠抗體 (2 選 1) 方案二買 3 送 1 以低價者計 Human CD marker 抗體 特定抗體, 買三支再送一支! 以低價者計 特定抗體詳細品項請洽當區業務 騰達行企業股份有限公司 UNIMED HEALTHECARE INC. 台北 TEL (02) 嘉義 TEL (05) 新竹 TEL (03) 台南 TEL (06) 台中 TEL (04) 高雄 TEL (07) 分子生物試劑類蛋白免疫試劑類細胞組織試劑類臨床檢查試劑類分析培養耗材類自動化分析儀類 騰達行客服專線