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1 2014 绎 Vol.16 No IBS-D 40 SD IBS-D AWR Claudin-1 AQP3 AQP8 Na + /K + -ATPase IBS-D AWR Claudin-1 AQP3 AQP8 Na + /K + -ATPase P 约 0.01 IBS-D AWR P 约 0.05 P 约 0.01 Na + /K + - ATPase Claudin-1 AQP3 AQP8 P 约 0.05 P 约 0.01 > > 3 IBS-D doi: /wst R285.5 A Irritable Bowel Syndrome IBS [1~3] IBS 5%~20.72% [4 5] IBS IBS with Diarrhea IBS-D IBS with Constipation, IBS -C Mixed IBS, IBS-MUnsubtyped IBS, IBS-U [6 7] IBS IBS IBS IBS [8~13] [14] IBS-D 元元元 y CB ZYSNXD- CC-HPGC-JD-014 :

2 绎 Sprague-Dawley SD h 20 依 2 益 50 豫 ~70 豫 mm 5mm 1.3 OLYMPUS Motie Med Claudin-1 Abcam ab15098 AQP3 Abcam ab AQP8 Abcam ab85909 ATP SANTA CRUZ SC EnVision Rabbit/M Dako K4008 DAB AR AL-chaer E D [15] IBS-D 8 2cm 0.2 ml 1 min 30 min SD 8 SD min [16] 7 SD min [16] 7 SD min AWR 3% 5cm 3cm 4 豫 24 h AWR AL-chaer E D [15] AWR mmhg 4 CRD CRD 20 s 4min 5 AWR 2 AWR [15] 0 CRD 1 CRD EnVision 0.01M ph min 0.3 豫 H 2 O 2 20 min 20% 30 min AQP3 AQP8 Claudin-1 ATP 37 益 2h EnVision 30 min DAB Motie Med

3 2014 绎 Vol.16 No SPSS 18.0 x 依 s One -way Analysis Of Variance One -way ANOVA Least Signifi 原 cant Difference LSD Games-Howell M min~ max P< IBS-D AWR mmhg CRD AWR P 约 mmhg CRD AWR P 约 0.05 P 约 mmhg CRD AWR P 约 0.05 P 约 mmhg CRD AWR P 约 0.05 CRD AWR 20 mmhg CRD AWR AWR P 约 IBS-D Claudin-1 AQP3 AQP8 Na + /K + -ATPase Claudin-1 AQP3 AQP8 Na + /K + -ATPase AQP3 AQP8 Claudin -1 Na + /K + -ATPase P 约 0.01 Claudin-1 AQP3 AQP8 P 约 0.05 P 约 0.01 Claudin -1 AQP3 P 约 0.05 P 约 0.01 Claudin ~4 1 AWR x 依 s mmhg n 依依依依依依依依依依依依依依依依殷殷 1.33 依依依依 0.33 殷殷 P 约 0.01 P 约 0.05 P 约 0.01 P 约 Claudin-1 AQP3 AQP8 Na + /K + -ATPase x 依 s n Claudin-1 AQP3 AQP8 Na + /K + -ATPase 依依依依依依依依依依依依依依依依殷 0.55 依依依依 0.07 殷 P 约 0.01 P 约 0.05 P 约 0.01 P 约

4 绎 1 Claudin-1 伊 AQP8 伊 AQP3 伊 Na + /K + -ATPase 伊

5 2014 绎 Vol.16 No.6 3 IBS-D [17] IBS Claudin-1 Wilcz-Villega E M [18] Caco-2 IBS Caco-2 Claudin-1 Claudin-1 [19] IBS Claudin-1 IBS-D Claudin -1 Claudin-1 IBS [20~22] IBS-D claudin-1 AQPs [23] AQP3 [24 25] AQP3 [24] AQP3 [26] AQP3 Caco- 2 C25 [25] AQP8 [27] Wang J P [28] IBS -D AQP8 AQP8 IBS-D IBS-D AQP3 AQP8 IBS-D AQP3 AQP8 [29 30] Na + /K + -ATPase ATP [31 32] IBS Na + /K + -ATPase [33] IBS Na + / K + -ATPase [34 35] IBS-D Na + /K + -ATPase Claudin-1 AQP3 AQP8 Na + /K + -ATPase IBS -D Claudin -1 AQP3 AQP8 Na + /K + - ATPase IBS-D [36 37] 30 IBS -D IBS-D [38] 猿 [39] [40] 10.6 滋 m CO 滋 m CO 2 SAMP8 Th1/Th2 [41~43] 3 IBS-D IBS IBS IBS-D CRD AWR Na + /K + -ATPase Claudin-1 AQP3 AQP8 IBS-D IBS-D IBS-D IBS-D AWR Claudin-1 AQP3 AQP8 > > 3 IBS-D 1265

6 绎 1 Longstreth G F, Thompson W G, Chey W D, et al. Functional bow 原 el disorders. Gastroenterology, 2006, 130(5) 颐 1480~ Chang J Y, Talley N J. An update on irritable bowel syndrome: from diagnosis to emerging therapies. Curr Opin Gastroenterol, 2011, 27(1) 颐 72~78. 3 Roisinblit K C. Irritable bowel syndrome in women. J Midwifery Wom Heal, 2013, 58(1) 颐 15~24. 4 Liu J, Hou X. A review of the irritable bowel syndrome investiga 原 tion on epidemiology, pathogenesis and pathophysiology in China. J Gastroenterol Hepatol, 2011, 26(3) 颐 88~93. 5 Zhou H, Li D, Cheng G, et al. An epidemiologic study of irritable bowel syndrome in adolescents and children in South China: a school-based study. Child Care Health Dev, 2010, 36(6) 颐 781~ Drossman D A. The functional gastrointestinal disorders and the Rome III process. Gastroenterology, 2006, 130(5) 颐 1377~ , 2011, 31 ( 5 ) 颐 587~ Park J W, Lee B H, Lee H. Moxibustion in the management of ir 原 ritable bowel syndrome: systematic review and meta -analysis. BMC Complement Altern Med, 2013,13 颐 Ma Y X, Liu X, Liu C Z, et al. Randomized clinical trial: the clinical effects of herb -partitioned moxibustion in patients with di 原 arrhoea-predominant irritable bowel syndrome. Evid Based Comple 原 ment Alternat Med, 2013, 2013 颐 ,,,..,2014,34(1) 颐 45~48. 11,,,..,1996,16(2) 颐 43~ Wu L Y, Bao C H, Ge L B, et al. Mild moxibustion at Tianshu (ST 25) decreases expression of prokineticin-1 and prokineticin re 原 ceptor -1 in colon tissue of rats with chronic visceral hyperalgesia. Neural Regen Res, 2011, 6(33) 颐 2600~ Zhou E H, Wang X M, Ding G H, et al. Suspended moxibustion relieves chronic visceral hyperalgesia and decreases hypothalamic corticotropin-releasing hormone levels. World J Gastroenterol, 2011, 17(5) 颐 662~ , 芃,,..,2013,8(8) 颐 845~ Al -Chaer E D, Kawasaki M, Pasricha P J. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation dur 原 ing post- natal development. Gastroenterology, 2000, 119(5) 颐 1276~ :,2003 颐 327~ ,2010,25(7) 颐 1062~ Wilcz-Villega E M, McClean S, O'Sullivan M A. Mast cell tryptase reduces junctional adhesion molecule-a (JAM-A) expression in in 原 testinal epithelial cells: implications for the mechanisms of barrier dysfunction in irritable bowel syndrome. Am J Gastroenterol, 2013, 108(7) 颐 1140~ Iraha A, Chinen H, Hokama A, et al. Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1. World J Gastroenterol, 2013, 19(33) 颐 5500~ Bertiaux-Vanda 颐 le N, Youmba S B, Belmonte L, et al. The expres 原 sion and the cellular distribution of the tight junction proteins are altered in irritable bowel syndrome patients with differences accord 原 ing to the disease subtype. Am J Gastroenterol, 2011, 106 (12) 颐 2165~ ,,. claudin-1.,2008,48(2) 颐 27~29. 22,,,. claudin-1,-3,-4.,2007,27 (9) 颐 1345~ Perez Di Giorgio J, Soto G, Alleva K, et al. Prediction of aquapor 原 in function by integrating evolutionary and functional analyses. J MembrBiol, 2014, 247(2) 颐 107~ Ikarashi N, Kon R, Iizasa T, et al. Inhibition of aquaporin-3 water channel in the colon induces diarrhea. Biol Pharm Bull, 2012, 35 (6) 颐 957~ Zhang W, Xu Y, Chen Z, et al. Knockdown of aquaporin 3 is in 原 volved in intestinal barrier integrity impairment. FEBS Lett, 2011, 585(19) 颐 3113~ Ikarashi N, Ushiki T, Mochizuki T, et al. Effects of magnesium sulphate administration on aquaporin 3 in rat gastrointestinal tract. Biol Pharm Bull, 2011, 34(2) 颐 238~ Calamita G, Mazzone A, Bizzoca A, et al. Expression and im 原 munolocalization of the aquaporin -8 water channel in rat gastroin 原 testinal tract. Eur J Cell Biol, 2001, 80(11) 颐 711~ Wang J P, Hou X H. Expression of aquaporin 8 in colonic epithe 原 lium with diarrhoea -predominant irritable bowel syndrome. Chin Med J (Engl), 2007, 120(4) 颐 313~ ,,,. 8.,2012,18(6) 颐 141~144 30,,,. 3.,2011,19(9) 颐 899~ Cereijido M, Contreras R G, Shoshani L, et al. The Na + -K + -AT 原 Pase as self-adhesion molecule and hormone receptor. Am J Physi 原 ol Cell Physiol, 2012, 302(3) 颐 C473~ Zhang L N, Li J X, Hao L, et al. Crosstalk between dopamine re 原 ceptors and the Na + /K + -ATPase(review). Mol Med Rep,2013,8(5) 颐 1291~ Kumar A R, Kurup P A. Hypothalamic digoxin and irritable bowel syndrome. Indian J Gastroenterol, 2001, 20(5) 颐 173~

7 2014 绎 Vol.16 No.6 34,,,. D-IBS.,2010,41(8) 颐 1332~ ,,,.ATP IBS.,2009,17(8) 颐 817~ ,. 40.,2011,31(2) 颐 153~ ,,,. ().,2009,29(2) 颐 111~ ,,,. IBS.,2010,25(12) 颐 2224~ ,,, 援援,2009,28(1) 颐 1~6 援 40,,,.CO 2.,2012,31(10) 颐 699~ ,,,. SAMP8 Th1/ Th2.,2012,27(5) 颐 1387~ ,,,. SAMP8.,2012,32(1) 颐 53~57. 43,,,. SOD MDA GSH-Px.,2011,4(6) 颐 413~415. Effect of Visceral Pain and Water Metabolism on IBS-D Model Rats by Moxibustion and Its Products Zhou Cili 1,WuLuyi 2,WuBeiling 3,LiuXiaoxu 1,LiuHuirong 1, Wu Huangan 1,ShiZheng 1, Chen Changle 2, Zhang Linshan 2,QianQinglan 1 (1. Shanghai Research Institute of A cupuncture and Meridian, Shanghai , China; 2. Shanghai Qigong Research Institute, Shanghai , China; 3. Shanghai Geriatric Institute of Chinese Medicine, Shanghai , China) Abstract: This study was aimed to investigate the effect of visceral pain and water metabolism on irritable bowel syndrome with diarrhea (IBS-D) model rats by moxibustion and its products. A total of 40 SD rats were randomly di 鄄 vided into the normal group, model group, moxibustion group, non-smoke moxibustion group, and moxa smoke group. IBS-D model was induced by colorectal distension with an inflatable balloon. The general condition of rats was ob 鄄 served. Visceral pain was assessed by abdominal withdrawal reflex (AWR) scores. The immunohistochemical method was used in the detection of claudin-1, AQP3, AQP8 and Na + /K + -ATPase protein expression. The results showed that compared with the normal group, the AWR scores, claudin-1, AQP3, AQP8 and Na + /K + -ATPase protein expres 鄄 sion in the colon were decreased in the model group (P < 0.01). The stool was loose, which was on hair around the anus. After moxibustion, non-smoke moxibustion and moxa smoke stimulation, the stool conditions of IBS-D model rats were improved. The AWR scores were decreased in different degrees (P <0.05orP < 0.01). Except Na + /K + - ATPase, expressions of claudin-1, AQP3 and AQP8 in colon were increased in different degrees (P <0.05orP < 0.01). The comprehensive therapeutic effect showed that moxibustion was better than non -smoke moxibustion; and non -smoke moxibustion was better than moxa smoke stimulation. It was concluded that the combination of warm stimulation, optical radiation and moxa smoke induced best effect in improving visceral pain and water metabolism on IBS-D model rats. Among them, warm stimulation and optical radiation may play the main role. Keywords: Irritable bowel syndrome with diarrhea, moxibustion, moxibustion products, visceral pain, water metabolism 1267

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<30302DB7E2C3E6CDC6BDE9A3A D32A3A92E4D4449> 绎阴元元 510515 Caco-2 Caco-2 P