Microsoft Word - 13——李宁_news_.doc

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1 海昆桑提取物对 2 型糖尿病大鼠的降血糖作用研究 1, 2, 李宁 3 1, 2,, 郭传龙 3 1,, 王立军 2 1, 2,, 李晓伟 3 1, 2,, 刘华 3 1, 2,, 徐琦 3, 1, 2, 3 史大永 (1., ; 2.,, ; 3., ) 摘要 : 为合理利用海洋资源探究海昆桑 ( 昆布 - 桑叶 - 山药 - 枸杞 - 肉桂复合 ) 提取物的降血糖作用, 通过腹腔注射链脲佐菌素 STZ 构建 2 型糖尿病大鼠模型, 随机分为模型组阳性对照组海昆桑乙醇提取物低中高剂量组以及水提物高剂量组, 分别以 0.5% CMC-Na 液二甲双胍 0.08 g/(kg d) 海昆桑乙醇提取物 0.2 g/(kg d) 0.4 g/(kg d) 0.8 g/(kg d) 海昆桑水提物 0.8 g/(kg d) 灌胃, 另随机取 10 只正常大鼠作为正常对照组 (0.5% CMC-Na 液 ), 持续给药 4 周给药后每周对各组大鼠的一般情况变化进行分析, 并利用其模型对海昆桑提取物进行空腹血糖试验以及糖耐量试验结果显示, 海昆桑提取物可以改善 2 型糖尿病高血糖症状, 且以乙醇提取物高剂量组降血糖作用为最佳 (P<0.01) 实验结果说明, 海昆桑提取物具有降低血糖的作用, 为降糖药物的研发或海洋保健食品的研制提供了新途径关键词 : 海洋资源 ; 海昆桑 ; 提取物 ; 降血糖 ; 2 型糖尿病中图分类号 : X55 文献标识码 : A 文章编号 : (2018) DOI: /hykx ,, [1],, 2015, 4.15,, 2040, 6.42, [2] ( ), 2,, Laminaria japonica Aresch. Ecklonia kurome Okam., [3] Morus alba L.; Dioscorea opposita Thunb. ; Lycium barbarum L. ; Cinnamomum cassia Presl, [3-8] 1 材料 1.1 仪器 (LT5028, ); ( ) ( ); DFY-300 ( ) 1.2 药品和试剂 (Solarbio, S8050); (Sigma, BCBT7573); ( ); CMC-Na( : ; : : (2016GSF115009) [Foundation: Key Research and Development Project of Shandong Province, No. 2016GSF115009] : (1992-),,,,, : , ln92710@163.com; (1988-),,,,,, : , gcl_cpu@126.com;,,, : , shidayong@qdio.ac.cn Marine Sciences / Vol. 42, No. 3 /

2 ); (); ( ); ( ), Laminaria japonica Aresch. Ecklonia kurome Okam. ;, Morus alba L. ;, Dioscorea opposita Thunb. ;, Lycium barbarum L. ;, Cinnamomum cassia Presl 1.3 动物 Wistar 80 (, ), 250~300 g,,, : SCXK ( ) , 2 方法 2.1 供试品的制备过程 [9-14], 120 g 1 cm 2,, g 120 g 60 g 30 g, 20, 3, 10, 80 4 h, 400,,, 120 g 1 cm 2,, g 120 g 60 g 30 g, 20, 95% 3, 10 95%, 70 4 h, 3,, 2.2 糖尿病大鼠模型的制备 Wistar 80, 1, 10,, 70, STZ(40 mg/kg) 2, STZ1 12 h,,,, 16.7 mmol/l 2 [15-16] 2.3 糖尿病大鼠分组及降血糖试验 70 ( 16.7 mmol/l) 60 2, 6 : ( ), 7, ; 0.5% CMC-Na ;,, 0.08 mg/kg; 0.8 g/kg, g/kg 4,, 2.4 葡萄糖耐量试验 4, 12 h,, 0 h ;, ( ) 0.5% CMC-Na 20 min, 2.5 g/kg, h 2.5 统计学方法 spss 19.0, t, x ± s P< 结果 3.1 给药后 ( 链脲佐菌素 ) 大鼠的一般变化情况分析 :,,, ; :,,,,, 102 / 2018 / 42 / 3

3 ,, ( 1), ;, ;, 3 4,, ( 2) 88% 表 1 海昆桑提取物对糖尿病大鼠摄食量的影响 ( x ± s, n=10) Tab. 1 Effect of Haikunsang extracts on food intake of diabetic rats ( x ± s, n=10) 24 h /g /(g/kg) ± ± ±8.56 * 56.73±6.34 * ± ±11.18 ( ) ±10.82* 41.65±8.65 * ( ) ± ±9.32 ( ) ± ±10.28 ( ) ± ±12.06 :, *(P<0.05), **(P<0.01); : (P<0.05), (P<0.01) 表 2 山药 - 昆布 - 桑叶 - 枸杞 - 肉桂提取物对糖尿病大鼠体重的影响 (m/g, x ± s, n=10) Tab. 2 Effect of Haikunsang extracts on body weight of diabetic rats(m/g, x ± s, n=10) /g ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±42 ( ) ± ± ± ± ±32 ( ) ± ± ± ± ±34 ( ) ± ± ± ± ±36 ( ) ± ± ± ± ± 海昆桑提取物对糖尿病大鼠血糖的影响,, ;,,, (P<0.05),, (P<0.01),, ( 3) 3.3 海昆桑提取物对正常大鼠糖耐量的影响, h ( 4) 4 讨论 2,, ( ), 2 2,,,,,,, 0.5 h, 0.5 h, 2 Marine Sciences / Vol. 42, No. 3 /

4 表 3 海昆桑提取物对糖尿病大鼠空腹血糖的影响 ( x ± s, n=10) Tab. 3 Effect of Haikunsang extracts on blood glucose of diabetic rats ( x ± s, n=10) (mmol/l) ± ± ± ± ± ± ±1.30 ** 22.25±1.36 ** 25.32±1.20 ** 28.23±1.32 ** 19.21± ±1.35 ** 20.33±1.33 ** 18.92±1.41 ** 17.60±1.42 ** ( ) 19.08± ±1.34 ** 26.88±1.35 ** 26.31±1.24 ** 23.98±1.52 ** ( ) 19.05± ±1.16 ** 21.25±1.09 * 20.99±1.05 ** 18.79±1.24 ** ( ) 19.09± ±1.42 ** 19.45±1.35 ** 18.19±1.04 ** 16.66±1.36 ** ( ) 19.03± ±1.32 ** 23.40±1.29 ** 22.45±1.05 ** 18.87±1.15 ** 表 4 海昆桑提取物对正常大鼠糖耐量的影响 (x ± s, n=10) Tab. 4 Effect of Haikunsang extracts on oral glucose tolerance test of diabetic rats( x± s, n=10) /(mmol/l) 0 h 0.5 h 1 h 2 h 6.51± ± ± ± ±1.32 >33.3 >33.3 > ± ± ± ±1.12 ( ) 23.98±1.52 >33.3 >33.3 >33.3 ( ) 18.79± ± ± ±1.16 ( ) 16.66± ± ± ±1.30 ( ) 18.87± ± ± ±1.28,,,, : [1],,. [J]., 2016, 40(2): Zhang Jinli, Zhang Luocheng, Gu Yunjuan. Research on coupling coordination between marine and regional economies in Jiangsu province[j]. Marine Sciences, 2016, 40(2): [2]. [M]. :, Zhang Jinding. Marine drugs and efficacious prescriptions[m]. Beijing: Traditional Chinese Medicine Classics Press, [3],,,. [J]., 2016, 40(1): Cui Cuiju, Li Yan, Liu Yanling, et al. Establishment of core collection for Saccharina japonica using molecular markers[j]. Marine Sciences, 2016, 40(1): [4],,,. [J]., 2012, 30(9): Chen Yuwei, Xu Maoyi, Zhao Fengquan, et al. Advances in studies on flavonoides extraction process and mechanism of hypoglycemic effect of Mulberry flavonoids[j]. Chinese Archives of Traditional Chinese Medicine, 2012, 30(9): [5],,. [J]., 2013, 11(6): Zhang Ou, Tan Zhiping, Li Yanping. Pharmacological action of mulberry leaf and its clinical application[j]. Guide of China Medicine, 2013, 11(6): [6]. [J]., 2013, 9(5): Yang Xiuxia. Advances in studies on chemical constituents and pharmacological activities of Dioscorea opposita Thunb[J]. Asia-Pacific Traditional Medicine, 2013, 9(5): [7],. [M]. :, 2009: Hu Xianguo, Hu Aiping. Chinese wolfberry[m]. Beijing: People s Military Medical Publisher. 2009: [8], ()[S]. :, 2005: Chinese Pharmacopoeia Commision, Chinese Pharmacopoeia (part 1)[S]. Beijing: Chemical Industry Press, 2005: [9],,,. [J]., 2005, 39(1): 104 / 2018 / 42 / 3

5 Liu Yong, Xing Chunli, Cheng Zhansheng, et al. Optimization of extraction technology of medicinal components from Morus alba L.Leaves[J]. Journal of Chemical Industry of Forest Products, 2005, 39(1): [10],,,. - 2 [J]., 2014, 33(6): Xu Peng, Sha Weiqiao, Lü Zhihui, et al. Effects of alcohol extract compound from Yam and Bitter Gourd on glucolipid metabolism of type 2 diabetes mellitus rats[j]. Genomics and Applied Biology, 2014, 33(6): [11] Kim S H, Hyun S H, Choung S Y. Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice[j]. Journal of Ethnopharmacology, 2006, 104(2): [12] Dvir I, Chayoth R, Sod-Moriah U, et al. Soluble polysaccharide and biomass of red microalga Porphyridium sp alter intestinal morphology and reduce serum cholesterol in rats[j]. British Journal of Nutrition, 2000, 84(4): [13],. [J]., 2004, 29(10): Sun Wei, Wang Huiming. The study on the effect of Thallus caminariae polysaccharides on the cholesterol-lowering effect on hyperlipidemia rats and its mechanism[j]. China Journal of Chinese Materia Medica, 2004, 29(10): [14],,,. 4 [J]., 2014, 36(6): Meng Qinghai, Yin Qiuyi, Guo Jing, et al.screening of hypoglycemic effects of 4 different mulberry leaves extracts[j].chinese Traditional Patent Medicine, 2014, 36(6): [15],,,. STZ [J]., 2010, 27(1): Nong Hui, Sheng Qingshou, Liang Jian, et al.studies on diabetic rats induced by Streptozotocin[J]. Journal of Guangxi Medical University, 2010, 27(1): [16] Srinivasan K, Viswanad B, Asrat L, et al. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for type 2 diabetes and pharmacological screening[j]. Pharmacological Research, 2005, 52(4): Hypoglycemic activity of Haikunsang mixture on rats with type 2 diabetes mellitus LI Ning 1, 2, 3, GUO Chuan-long 1, 2, 3, WANG Li-jun 1, 2, LI Xiao-wei 1, 2, 3, LIU Hua 1, 2, 3, XU Qi 1, 2, 3 1, 2, 3, SHI Da-yong (1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao , China; 2. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao , China; 3. University of Chinese Academy of Sciences, Beijing , China) Received: Nov. 15, 2017 Key words: marine resource; Haikunsang; extracts; hypoglycemia activity; type 2 diabetes mellitus Abstract: To investigate the potential for utilizing a marine resource, we investigated the hypoglycemia activity of extracts derived from a mixture of Haikunsang in rats with type 2 diabetes mellitus. We divided the streptozotocin (STZ)-induced rats into a model group, positive control group, and low, medium, and high dose groups of alcoholic extracts of Haikunsang, as well as a high dose group of aqueous extracts of Haikunsang. These groups were fed by gavage with 0.5% CMC-Na, 0.08 g kg 1 d 1 metformin, 0.2, 0.4, 0.8 g kg 1 d 1 alcoholic extracts of Haikunsang, and 0.8 g kg 1 d 1 aqueous extracts of Haikunsang. As a normal control group, we randomly selected another 10 healthy rats, which were fed by gavage with 0.5% CMC-Na. We consecutively treated both the diabetic and healthy rats for 4 weeks, and individually recorded their body weights, food intakes, psychiatric status, and other changes. We found the diabetic rats to exhibit typical symptoms. After Haikunsang extract administration for 4 weeks, we recorded their blood glucose concentrations via an oral glucose tolerance test (OGTT). We found the blood glucose concentrations of most rats had significantly decreased in the high dose groups that had received alcoholic extracts of Haikunsang (0.8 g kg 1 d 1, P<0.01). These results suggest that extracts derived from a mixture of Haikunsang play a regulatory role in the blood glucose levels of diabetic rats, thereby providing a new approach to the development of hypoglycemic drugs and marine health foods. ( 本文编辑 : 康亦兼 ) Marine Sciences / Vol. 42, No. 3 /

TGF-β AngⅡ B SD ~ 220g SPF. SCXK No SYXK ~ 25 40% ~ 70% OR37G-C

TGF-β AngⅡ B SD ~ 220g SPF. SCXK No SYXK ~ 25 40% ~ 70% OR37G-C 2017 7 43 4 Journal of Southwest Minzu University Natural Science Edition Jul. 2017 Vol. 43 No. 4 doi10. 11920 /xnmdzk. 2017. 04. 008 1 1 1 2 1 1 3 1. 610212 2. 6100413. 610031 AngⅡ TGF-β. SD 60 180 ~

Microsoft Word - 13——李 宁_news_.doc

Microsoft Word - 13——李宁_news_.doc