Vol brwg 2. 3 Fusarium graminearum Z h 0. 1 g 2 g 5 g 15 g 1000 ml 0. 1 g 10 g 15 g 1000 ml PDA 200 g

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1 Nat Prod Res Dev * A B A B A B R A Production of Dragon's Blood in Dracaena cambodiana Plants by Inoculation of Fusarium graminearum XU Xiao-feng 1 2 LI Shang-zhen 1 SONG Qi-shi 1* 1 Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Kunming China 2 The Graduate School of the Chinese Academy of Science Beijing China Abstract Dragon's blood is a precious traditional medicine. Physical damage and fungi induction are two main factors affecting the production of dragon's blood. In this study fungi induction was used to produce dragon's blood. Loureirin A and loureirin B the major compounds of the dragon's bloods induced were analyzed by HPLC to evaluate the results of each treatment. The results showed that the contents of loureirin A of the dragon's bloods induced by different treatments were similar while the contents of loureirin B of the dragon's bloods induced by inoculating fungus Fusarium graminearum and exposing wound was the highest. The treatment of inoculating fungus and exposing wound was the most efficient way to induce dragon's blood. The HPLC fingerprints showed that the dragon's bloods induced were similar to the dragon's blood drug. This may add weight to the feasibility of artificial production of dragon's blood. Key words dragon's blood Fusarium graminearum HPLC loureirin A loureirin B HPLC fingerprint Dracaena cochinchinensis Lour. S. C. Chen D. cambodiana Pierre ex Gagnep brwg 3 A B ZK171K AA * Tel songqs@ xtbg. ac. cn

2 Vol brwg 2. 3 Fusarium graminearum Z h 0. 1 g 2 g 5 g 15 g 1000 ml 0. 1 g 10 g 15 g 1000 ml PDA 200 g 20 g 15 g 1000 ml 1. 2 A 30 min 10 ml B mg /ml A mg /ml B 待测样品溶液配制 1. 3 Waters AL mg HS10260D min 10 ml 10 min μm Waters 1525 Wa- PDA ters Symmetry C mm 150 mm 5 μm brwg 28 5 Waters d = = d 1 ml /min 274 nm 24 h A B A B HPLC h 2. 4 HPLC 标准品配制 A 2 mg B 1 mg 100 mg 色谱条件 98-1-B SW-CJ-2FD 30 min 10 ml 10 min GNP-9080 HZQ μm F160 BüCHI R 血竭药品溶液配制 A 28 min B 30 min 标准曲线制作 cm 1. 2 cm /3 1 μl 2 μg

3 98 Vol. 25 A 20 ~ 400 ng B 10 ~ 200 ng A B Y = X R 2 = Y = X R 2 = 试验样品检测 20 μl A B 数据处理 A B A B mg /g = HPLC mg /ml * g / g * 100 ml 5 A B HPLC 2 SPSS HPLC 30 min One-Way ANOVA a b A B 2 A B 30 min A B A 3. 1 B A 1 PDA 1 B 2 B 1 3 B 3. 3 HPLC HPLC 25 min 25 min 4 1 Fig. 1 brwg 3. 1 A B Maria S. O. 6 F. Lecompte 7 A Fungi grown on three different mediums B

4 Vol Table 1 1 A B ± n = 5 The contents of loureirin A and loureirin B detected in dragon's bloods induced by different treatments mean ± SD n = 5 Sample number Treatments A Contents of loureirin A mg /g B Contents of loureirin B mg /g Extraction rates % 0 No hole no inoculation ± a ± a ± Hole no inoculation bare ± a ± b ± Hole inoculation bare ± a ± c ± Hole inoculation sealed ± a ± bc ± 1. 5 P < Note Data in the same column with different superscripts means the data have significant differences P < HPLC brwg Fig. 4 Overlaid HPLC fingerprints of different samples B 2 S HPLC 0 HPLC HPLC A A B B S HPLC fingerprint of the dragon's blood drug 0 HPLC fingerprint of blank control indicated the fingerprints of dragon's blood from treatment A loureirin A B loureirin B 2 3 B 3 1 B 2 B B B B B 11 2 B 3. 2 A B B B 1 A B B A A B B 5 B 1 2 A B 3 1 A B

5 100 Vol Maria SO Maria LR Germán GB et al. Influence of water activity and temerature on growth and mycotoxin production by Alternaria alternata on irradiated soya beans. Int J Food Microbiol Lecompte F Abro MA Nicot PC. Contrasted responses of 1 Gupta D Bleakley B Gupta RK. Dragon's blood botany chemistry and therapeutic uses. J Ethnopharmacol Cai XT Xu ZF. Study on plant resources of dragon's blood resin in China. Acta Bot Yunnanica Yang XH Deng SM Fan LX. Exploitation to the resources of Dracaena cambodiana plants. Nat Sci J Hainan Univ Jiang DF Ma P Yang J et al. Formation of blood resin in abiotic Dracaena cochinchinensis 5 Wang XH Yang LL Jose GL et al. Enhanced dragon's blood production in Dracaena cochinchinensis by elicitation of Fusarium oxysporum strains. J Med Plants Res Botrytis cinerea isolates developing on tomato plants grown under different nitrogen nutrition regimes. Plant Pathol Wang XH. Flavones formed from xylem stem of Dracaena cochinchinensis by co-culture of fungus strain and bacteria strain. Nat Prod Res Dev Nakashima K Abe N Kamiya F et al Novel flavonoids in dragon's blood of Daemonorops draco Helv Chim Acta Gonzalez AG Leon F Hernandez JC et al. Flavans of dragon's blood from Dracaena draco and Dracaena tamaranae. Biochem Syst Ecol inoculated with Fusarium 9568D. Chin J App Ecol Deng JG Huang HB Nong YQ. Experimental study on pharmacological effects of lourerin B. Guangxi J Tradit Chin Med 櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵 ( 上接第 95 页 ) 8 Vuong QV Stathopoulos CE Nguyen MH et al. Isolation of green tea catechins and their utilization in the food industry. Food Rev Int Baumann D Adler S Hamburger M. A simple isolation method for the major catechins in green tea using High-speed countercurrent chromatography. J Nat Prod Loibner H. Seidl G. A low-cost medium-pressure liquid chromatography system for preparative separations. Chromatographia Claeson P Tuchinda P Reutrakul V. Some empirical aspects on the practical use of flash chromatography and medium pressure liquid chromatography for the isolation of biologically active compounds from plants. J Sci Soc Thailand Weber P Hamburger M Schafroth N et al. Flash chromatography on cartridges for the separation of plant extracts rules for the selection of chromatographic conditions and comparison with medium pressure liquid chromatography. Fitoterapia Liu QS Zhang ZQ Huang XL et al. Research on Chinese minority traditional medicine and new chromatographic techniques. J Cent Univ Nal Nat Sci Ed Davis AL. Cai Y Davis AP et al. 1 H and 13 C NMR assignments of some green tea polyphenols. Magn Reson Chem Lee MW Morimoto S Nonaka GI et al. Flavan-3-ol gallates and proanthocyanidins from pithecellobium lobatum. Phytochemistry

1449 1 相对校正因子的相关方法 1.1 内标多控法研究发现, 在中药各成分间存在着一定的比例关系, 只要各成分的量符合该比例关系, 中药的多 [6] 指标质量控制可以依此法

1449 1 相对校正因子的相关方法 1.1 内标多控法研究发现, 在中药各成分间存在着一定的比例关系, 只要各成分的量符合该比例关系, 中药的多 [6] 指标质量控制可以依此法 1448 相对校正因子在中药多指标测定中的应用研究进展 1, 罗祖良 2, 仇峰 1 1,, 韦日伟 2 1,, 覃禹 2, 张坤 1, 覃洁萍 2, 杨美华 1. 北京协和医学院药用植物研究所, 中草药物质基础与资源利用教育部重点实验室, 北京 100193 2. 广