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1 6 6 Vol. 6 No Journal of Food Safety and Quality Jun., 2015 β- 赵范, 刘会平 *, 刘晓庆, 张晨萍, 王宇, 徐娇娇 (, ) 摘要 : 目的 β- 方法, (SOD)(GSH-PX) (MDA) 结果 25.39% β-, SOD GSH-PX, MDA 结论 β-, 关键词 : ; β- ; ; Effect of oat bran β-glucan on hypoglycemic and antioxidant ZHAO Fan, LIU Hui-Ping *, LIU Xiao-Qing, ZHANG Chen-Ping, WANG Yu, XU Jiao-Jiao (College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin , China) ABSTRACT: Objective To study the effects of oat bran β-glucan on hyooglycemic and antioxidant. Methods The mice were divided into normal group and model group. The models of experimental diabetes were built by injecting Alloxan in mouse. The body weight, food intake, water intake, blood glucose levels, the glucose tolerance, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) in the liver, and organ index of mice were analyzed. Results The high dosage group could reduce the average amount ingestion and water intake and increase the average weight of diabetic mice obviously, and the glucose levels of high dosage group were 25.39%. Oat bran β-glucan made no significant effect on normal mice s weight, water intake, food intake, blood glucose level, and glucose tolerance. Compared with control group, model group in mice the area under blood glucose curve, SOD, and GSH-PX activity were increased significantly, and the body MDA content was decreased significantly. Conclusion The results indicate that oat bran β-glucan has no effect on normal mice but can decrease the blood glucose levels of diabetic mice and improve the antioxidant capacity of diabetic mice. KEY WORDS: oats bran; β-glucan; hypoglycemic effect; antioxidant 1 引言,,, β-, [1] β- * 通讯作者 :,, liuhuiping@tust.edu.cn *Corresponding author: LIU Hui-Ping, Professor, Tianjin University of Science and Technology, No, 29, the Thirteenth Street, Economic Development Zone, Tianjin , China. liuhuiping@ tust.edu.cn

2 2132 6, [2] β- [3] [4] [5], β-, [7],,,, β-, 2 材料与方法 2.1 材料与试剂 : β- : β-, 66, ph 9.0, 500 W 20 min,, β-, ;, SCXK-( ) 仪器 MK-3, ; 96, ;, ;, ;, ;, ;, 2.3 试验小鼠处理 :, 10, 12 h 220 mg/kg bw,, 3 d, 12 h, 11.1 mmol/l [8], (),, ; 200 mg 400 mg 600 mg/kg bw, 1 ; 1, 500 mg/kg bw, 28 d 29 d,, (SOD) (MDA) (GSH-PX) 2.4 燕麦麸 β- 葡聚糖降血糖试验小鼠饮水量和摄食量的测定 ( ), 24 h, 4, 小鼠血糖值的测定, 12 h, 小鼠耐糖量测定 4, 3 h, 2 g/kg bw 0 min 30 min 60 min 120 min [9],, [10] : =0.25 (0 h h +3 2 h ) 小鼠肝脏抗氧化能力的测定,,,, [11,12] (1) (SOD) :, (g): (ml)=1:9 9,, 2500~3000 r/min, 10 min, 10% (2) (GSH-PX) SOD,, 10% 5% 1%, 5%, (GSH-PX) (3) (MDA) SOD, 5%, 2.5 数据分析 SPSS 18. 0, ± ( x ±s), P 0.05

3 6, : β 结果与分析 3.1 燕麦 β- 葡聚糖对小鼠体重的影响燕麦 β- 葡聚糖对糖尿病小鼠体重的影响 1,,,, β- 3.2 燕麦麸 β- 葡聚糖对小鼠饮水量的影响,,, 3,, β-, β-, β-,, β-,,, β- 1 β- Fig. 1 Effect of oat bran β-glucan on the body weight of diabetic mice 3 β- Fig. 3 Effect of oat bran β-glucan on the water intake of mice and diabetic mice 燕麦麸 β- 葡聚糖对正常小鼠体重的影响 2,,, β- 3.3 燕麦麸 β- 葡聚糖对小鼠摄食量的影响 4,, β-, β-,,, β-,,, β- 3.4 小鼠血糖水平测试结果 2 β- Fig. 2 Effect of oat bran β-glucan on the body weight of mice 1,, β-,

4 2134 6,, β-,,, β-, β %, β- 4 β- Fig. 4 Effect of oat bran β-glucan on the food intake of mice and diabetic mice 3.5 小鼠耐糖量测试结果,, [13] 正常小鼠耐糖量测定结果 h,,,,, 2 h,,, [14] β-,, β 糖尿病小鼠耐糖量测定结果 3, 0.5 h,,,, β-, Table 1 表 1 燕麦麸 β- 葡聚糖对正常小鼠和糖尿病小鼠血糖的影响 Effect of oat bran β-glucan on fasting blood glues of mice and diabetic mice (mmol/l) (mmol/l) (mmol/l) ±0.50 a 3.96±0.42 a 3.96±0.63 a 3.58±0.45 a 19.14±1.57 b 19.12±1.67 b 19.14±1.45 b 19.06±1.72 b 19.38±1.36 b ±0.74 a 3.25±1.08 a 3.38±0.78 a 3.26±1.14 a 19.8±1.68 b 18.4±2.42 bc 17.94±2.52 b 18.16±2.05 b 18.06±2.95 bc ±0.56 a 3.45±0.42a 3.65±0.81 a 3.26±0.98 a 20.8±1.84 b 17.98±2.37 b 17.2±1.72 b 17.09±1.30 b 16.86±1.72 bc ±0.66 a 3.85±0.58 a 3.59±0.72 a 3.65±0.64 a 22.1±2.74 b 17.07±1.57 b 16.11±2.04 b 15.98±1.38 bc 15.59±2.25 bc ±0.70 a 3.27±0.81 a 3.67±0.66 a 3.62±0.92 a 22.9±2.51 b 16.02±2.44 b 15.00±2.49 bc 14.69±1.97 bc 14.28±2.31 bc * :, P>0.05;, P<0.05 Table 2 表 2 燕麦麸 β- 葡聚糖对正常小鼠耐血糖的影响 Effect of oat bran β-glucan on oral glucose tolerance test of mice 0 h 0.5 h 2 h (h mmol/l) 7.1± ± ± ± ± ± ± ± ± ± ± ± ± ±1.24* 6.65± ±1.98 * * : ; * (P<0.05)

5 6, : β- 2135, 2 h,, β-,,,,, β- 3.6 小鼠肝脏超氧化物歧化酶 (SOD) 的测定 (SOD), SOD,, SOD [15] β- SOD 4, SOD, SOD,,,, β- SOD 3.7 小鼠肝脏丙二醛 (MDA) 的测定 5 (MDA), (MDA)(MDA), β-, MDA, β- MDA 3.8 小鼠肝脏谷胱甘肽过氧化物酶 (GSH-PX) 的测定 6 (GSH-PX), (GSH-PX), (GSH-PX),, β- GSH-PX Table 3 表 3 燕麦麸 β- 葡聚糖对糖尿病小鼠耐血糖的影响 Effect of oat bran β-glucan on oral glucose tolerance test of diabetic mice 0 h (mmol/l) 0.5 h (mmol/l) 2 h (mmol/l) (h mmol/l) 23.6± ± ± ± ± ± ± ± ± ±4.24* 10.15±4.04* 50.75± ± ±4.04** 9.6±3.65* 42.85±4.57** 15± ±4.36** 8.8±3.75** 39.05±6.57** * : * P 0.05 ** P 0.01 表 4 燕麦麸 β- 葡聚糖对小鼠 SOD 活性的影响 Table 4 Effect of oat bran β-glucan on SOD activity in mice (U/mL) (U/mL) (U/mL) (U/mL) (U/mL) 70.96± ± ± ± ±3.82 a 54.66±4.08 a 57.22±2.99 a 58.50±3.126 ab 61.15±3.28 ab * : a, b

6 表 5 燕麦麸 β- 葡聚糖对小鼠 MDA 含量的影响 Table 5 Effect of oat bran β-glucan on MDA content in mice (nmol/mgprot) (nmol/mgprot) (nmol/mgprot) (nmol/mgprot) (nmol/mgprot) 2.95± ± ± ± a ± ±0.80 a 2.97±0.42 a 2.47±0.39 ab 2.21±0.32 ab * a, b 表 6 燕麦麸 β- 葡聚糖对小鼠 GSH-PX 活性的影响 Table 6 Effect of oat bran β-glucan on GSH-PX activity in mice ( ) ( ) ( ) ( ) ( ) ± ± ± a ± a ± ab ± ab ± ab ± ab ±28.8 * a, b 4 结论 β-, β-,,, (SOD) (GSH-PX), (MDA), β- ;, β-, β-,, 参考文献 [1]. [J]., 2005, (3): Kuang W. Physiological effect and its application in food industry of soluble dietary fiber [J]. Guangzhou Chem, 2005, (3): [2] Tosh SM, Wood PJ, Wang Q, et al. Structural characteristic and theological properties of partially hydrolyzed oat β-glucans: the effect of molecular weight and hydrolysis method [J]. Carbohyd Polym, 2004, 55: [3],,,. β- [J]., 2012, 33(17): Wu SH, Shen Q, Tan B, et al. Research progress in oat β-glucan on the health effects of intestinal [J]. Sci Technol Food Ind, 2012, 33(17): [4],,. β- [J]., 2013, 12(18): Pang CX, Wang LM, Liu J. Research overview of the oat β-glucan functions in decrease blood sugar and blood fat [J]. Acad Period Farm Prod Process, 2013, 12(18): [5],,,. [J]., 2014, (2): Zhang M, Zhang ML, Guo J, et al. Summary of distribution, production, nutritional and physiological value of avena sativa l. in china [J]. Inner Mongolia Agric Sci Technol, 2014, (2): [6],,. [J]., 2007, 28(10): Luo ZY, Feng L, Wu MC. Hypoglycemic and antilipemic effects of plant polysaccharides [J]. Food Sci, 2007, 28(10): [7],,,. [J]., 2011, 42(3): Chen JG, Bu WL, Lai WQ, et al. Hypoglycemic effects and mechanism of mulberry leaves polysaccharide [J]. Chin Tradit Herbal Drug, 2011, 42(3): [8],,,. [J]., 2006, 28(5): Xu B, Dong Y, Zhang HH, et al. The anti hyperglycemic effects of polysaccharide form momordica charantia in stz-induced diabetic mice [J]. J Nutrit, 2006, 28(5): [9] Liu M, Wu K, Mao XQ, et al. Astragalus polysaccharide improves insulin sensitivity in KKAy mice: Regulation of PKB/GLUT4 signaling in skeletal muscle [J]. J Ethnopharmacol, 2010, 127:

7 6, : β [10] Gomez-lopez VM, Devlieghere F, Bonduelle V. Intense light pulese decontamination of minimally processed vegetables and their shelf-life [J]. Int J Food Microbiol, 2005, 103: [11] Maritim AC, Sander RA, Watkins JB. Diabetes, oxidative stress, and antioxidant: a review [J]. J Biochem Mol Toxicol, 2003, 17(1): [12] Shanmugam KR, Mallikarjuna K, Nishanth K, et al. Protective effect of dietary ginger on antioxidant enzymes and oxidative damage in experimental diabetic rat tissues [J]. Food Chem, 2011, 124(4): [13] Bothara SB, Singh S. Thermal studies on natural polysaccharied [J]. Asian Pac J Biomed, 2012, 2(2): S1031 S1035. [14]. [D]. :, Fu J. Hypoglycemia effect and the mechanism of mixture of Apostichopus and Cordyceps militaris [D]. Qingdao: Ocean University of China, [15] Huang SJ. Antioxidant properties of methanolic extracts from Agaricus blazei with various doses of gamma-irradiation [J]. LWT-Food Sci Technol, 2006, 39: 作者简介 ( 责任编辑 : 白洪健 ) 赵范, 硕士研究生, 主要研究方向为动物资源开发与功能食品 @qq.com 刘会平, 教授, 主要研究方向为动物资源开发与功能食品 liuhuiping@tust.edu.cn

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗 1338 中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用陈菲 1 2,, 艾国 3, 盛柳青 1, 麻佳蕾 1, 黄正明 1. 金华职业技术学院医学院, 浙江金华