Microsoft Word 黄远英_二校 出4992

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1 7 12 Vol. 7 No Journal of Food Safety and Quality Dec., 2016 黄远英 *, 汪玉芳 (, ) 摘要 : 目的 方法 3, g/kg BW 30 d 结果 (P<0.05), (P<0.05), (P<0.05) 结论 关键词 : ; ; ; NIH Study on alleviating physical fatigue effect of Polygonatum-maca tablet HUANG Yuan-Ying *, WANG Yu-Fang (By-Health Co., Ltd., Guangzhou , China) ABSTRACT: Objective To study the effect of Polygonatum-maca tablets on alleviating physical fatigue of mice. Methods The experimental mice were divided into 3 dose groups treated with low, medium and high dose of Polygonatum-maca tablets and a negative control group. The doses of gastric lavage of 3 dose groups were 0.3, 0.6, 1.2 g/kg BW, respectively. The loading swimming test, serum urea, serum lactic acid and hepatic glycogen detection of mice were conducted after the treatment with Polygonatum-maca Tablets for 30 days consecutively. Results The loading swimming time of mice in high dose group was longer than that of control group (P<0.05). The content of hepatic glycogen of mice in medium and high dose group was higher than that in control group (P<0.05). And the area under the curve of serum lactic acid of mice in high dose group was less than the control group (P<0.05). Conclusion The Polygonatum-maca tablets have the function of alleviating physical fatigue of mice under the conditions of this experiment. KEY WORDS: Polygonatum; maca; alleviation of physical fatigue; NIH mice 1 引言,,, (2015 ) [1] (Polygonatumkingianum Coll.et Hemsl.) (Polygonatumsibiricum Red.) (Polygonatumcyrtonema Hua.),, [1,2], [3,4] * 通讯作者 :, yuangenliang405@163.com *Corresponding author: HUANG Yuan-Ying, By-Health Co, Ltd., Guangzhou , China. yuangenliang405@163.com

2 [5] (Lepidiummeyenii, maca) (Brassicaceae) (Lepidium), 3500~4500 m [6,7], [7] (,, ), [6,8], [7,9] [7],,,, 2 材料与方法 2.1 主要仪器与试剂 JEA3001 ( 0.1 g, ); BS223S ( g, ()); ( 50 cm 35 cm 36 cm); 7020 ( HITACHI ); Multiskan Go ( Thermo ); Centrifuge 5418 ( Eppendorf ); Ms 3 digital ( IKA ); Lactate Scout ( EKF ) ( , ); ( , ); /( , ); ( , ) 2.2 实验动物 NIH, SPF,, 17.1~25.6 g; : SYXK( ) 样品, 900 mg,, 3.6 g 2.4 剂量选择和受试物给予方式 剂量选择, : , g/kg BW, 受试物给予方式, 0.2 ml/10 g 受试物配制 g,, 10 ml,, 3 [10] 2.5 试验方法, 40,, d, : (1) 30 min, 5% 35 cm, 25,, (2) 30 min, min, 60 min,, 1 ml( ), 3000 r/min 10 min,, (blood urea nitrogen, BUN) (creative kinase, CK) (3) 30 min,,, -80 ( 100 mg), (mg:μl=1:3),, 20 min,,, 5 min, 620 nm (optical density, OD), : (4) 30 min,, ; min, 2, ; 20 min 3, 3 : =5 ( +3 0 min min ) 2.6 统计分析及结果判定 统计方法

3 12, : 4993 ; ± ( x s ), SPSS 21.0, LSD, (1),, (2),, (3),, (4),, 综合判定, 3 2, 3 结果与分析 3.1 小鼠负重游泳试验 1,, (P 0.05), (P<0.05),, 3.2 小鼠血清尿素测定试验 2,, (P 0.05), (CK) (BUN)(P 0.05),,,,,,, (P 0.05) 3.3 小鼠肝糖原测定试验 3,, (P>0.05), (P>0.05), 表 1 黄精玛咖片对小鼠负重游泳试验的影响 (n=10) Table 1 Effect of Polygonatum-maca tablet on the loading swimming test of mice (n=10) (g) (min) 20.8± ± ± ± ±3.9 44± ± ± ± ± ±4.4 42± ± ± ± ± ±3.1 48± ± ± ± ± ±3.0 56±10 * : *(P<0.05) 表 2 黄精玛咖片对小鼠血清尿素测定的影响 (n=10) Table 2 Effect of Polygonatum-maca tablet on the assays for serum urea of mice (n=10) (g) CK (U/L) BUN (mmol/l) 21.8± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±952

4 表 3 黄精玛咖片对小鼠肝糖原测定的影响 (n=10) Table 3 Effect of Polygonatum-maca tablet on liver glycogen concentration of mice (n=10) (g) (mg/g ) 21.8± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±1.05 * ± ± ± ± ± ±1.60 * : *(P<0.05) 表 4 黄精玛咖片对小鼠血乳酸含量测定的影响 (n=10) Table 4 Effect of Polygonatum-maca tablet on blood lactic acid content determination of mice (n=10) (g) 19.2± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±26.1 * : *(P<0.05) (P<0.05),, ;,,,,, 3.4 小鼠血乳酸测定试验 4,, (P 0.05), (P<0.05),,,,, (P 0.05),, 4 结论,,,,,,,,, 30 d,,,, 参考文献 [1]. (2015 ) [M]. :, 2015, Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (The 2015 edition) The first edition [M]. Beijing: China Medical Science Press, 2015, [2],,,. [J]., 2011, 22(6): Shi J, Zhao Y, Lei Y, et al. Study on crude polysaccharide from Rhizoma polygonati on anti-fatigue and anti-aging [J]. Lishizhen Med Materia Medica Res, 2011, 22(6): [3],. [J]., 2009, 13(7):

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