3 : 551,, - (PAS) ( ) B(SBB) (AKP) (ACP), NaF(1.5 mg/ml) (DAB)(Peroxidase, Pox), 90 10min -α- (ANAE), NaF(1.5 mg/ml) PAS : +, 1 9 ; ++, 10 ; +++, PAS

庆厉郎
7 years ago
Views:

1 35 3 Vol. 35, No ACTA HYDROBIOLOGICA SINICA May, DOI: /SP.J 金丽赵娜黄林周传江张耀光 (,,, ) STRUCTURE AND CYTOCHEMICAL CHARACTERISTICS OF BLOOD CELLS IN MYXOCYPRINUS ASIATICUS JIN Li, ZHAO Na, HUANG Lin, ZHOU Chuan-Jiang and ZHANG Yao-Guang (Key Laboratory of Aquatic Organism Reproduction and Development (Southwest University), Ministry of Education; The Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University; Chongqing, , China) : ; ; ; ; DiI-C 18 Key words: Myxocyprinus asiaticus; Blood cell; Structure; Cytochemistry; DiI-C 18 中图分类号 : Q246;Q248 文献标识码 : A 文章编号 : (2011) Myxocyprinus asiaticus(bleeker) (Cypriniformes) (Catostomidae) 13 70,,,,, [1 3],,,,,, 1 1.1,, 1d( 72h,, ) ( : mm, : g) ,,,,, Wright-Giemsa (W-G), 10 Nikon 80i IPP 5, 4000, 50 ±SE, 1500 r/min 10min, ( ), 2.5% (4 ) 30min, ( ), PBS (ph 7.4, 4 ) 3 2.5% 4 2h, PBS (ph 7.4) 3, 1%,, 618, LKB-5,, TECNAI10 收稿日期 : ; 修订日期 : 基金项目 : (CSTS, 2009BA1053); (SWU109001) 作者简介 : (1977 ),, ;, ; jinll@swu.edu.cn 通讯作者 :, zhangyg@swu.edu.cn

2 3 : 551,, - (PAS) ( ) B(SBB) (AKP) (ACP), NaF(1.5 mg/ml) (DAB)(Peroxidase, Pox), 90 10min -α- (ANAE), NaF(1.5 mg/ml) PAS : +, 1 9 ; ++, 10 ; +++, PAS : +, ; ++, 1/2 ; +++, 2/3 ACP : +, 1 2 ; ++, 5 ; +++, ACP PAS ANAE : +, 1/2 ; ++, ; +++, DiI-C 18 [4], DiI-C 18 [5],,,, DiI-C 18 : 1 ml, DiI-C 18 ( 10 μmol/l), 2min, (0.2 mmol/l ADP), Nikon 80i (100W) G-2A( D535/50), CCD, JPEG 2 2.1, ( 1),,, ( Ⅰ-1),,, ( Ⅰ-2), ( ) ( ) ( Ⅰ-3),,,,, W-G : (Ⅰ),, 2 3 ( Ⅰ-4),, ( Ⅰ-24) (Ⅱ),,, ( Ⅰ-5), ( Ⅰ-24) (Ⅲ),,, ( Ⅰ-4) ( Ⅰ-4), S,,,,,,,,, ( Ⅰ-7),, Cell types Erythrocyte Granulocyte Monocyte Lymphocyte Thrombocyte Tab. 1 Percent (%) 1.89±0.09 表 1 胭脂鱼外周血细胞数量百分比及细胞大小 The percent and sizes of blood cells in Myxocyprinus asiaticus Long diameter of cell (μm) Short diameter of cell (μm) Long diameter of neucleus (μm) Short diameter of neucleus (μm) 97.04± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.10 : ± ; Note: Data in the table are all shown as means ± SE; suggest cells have irregular or segmental nucleus

3 552 35,,,, ( Ⅰ-6),,,,,, 4,, ( Ⅰ-4 7),,,,, 2.2,,,,,,, ( Ⅱ-1),,,,,, ( ),, Ⅰ Ⅱ Ⅰ,,,, ( Ⅱ-2 5),, ( Ⅱ-4), Ⅰ 2, Ⅰ 0.5 µm,, ; Ⅱ, ( Ⅱ-5) Ⅱ, µm ( Ⅱ-6),,,,,, Ⅰ,,,,, ( Ⅱ-7 8),, ( Ⅱ-9),,,,, ( Ⅱ-10), ( ),,,,,, ( Ⅱ-11), ( Ⅱ-12), 2.3 2, PAS, Ⅱ( Ⅰ-9), Ⅰ Ⅲ ( Ⅰ-8 10), ( Ⅰ-11) Cell types Tab. 2 表 2 胭脂鱼外周血细胞的细胞化学特征 The cytochemical characteristics of blood cells in Myxocyprinus asiaticus B SBB PAS AKP ACP POX ANAE Erythrocyte + Ⅰ Granulocyte-Ⅰ Ⅱ Granulocyte-Ⅱ Ⅲ Granulocyte-Ⅲ Monocyte Lymphocyte + + (+) Thrombocyte + + (+) : ; +++ ; ++ ; + ; (+) ; Note: express negative reaction; +++ express strong positive reaction; ++ express positive reaction; + express weak positive reaction; (+) express positive reaction in part of the cells; express variation range

4 3 : 553 ( Ⅰ-11) ( Ⅰ-12), SBB (ACP) ACP, ACP ( Ⅰ-13) Ⅰ Ⅲ ACP( Ⅰ-14), Ⅱ ACP ( Ⅰ-15) ACP ( Ⅰ-16), ( Ⅰ-17) ( Ⅰ-13) ACP, (ANAE) ( Ⅰ-18 20),, ( Ⅰ-21) ANAE ( Ⅰ-22) ( Ⅰ-18) ( Ⅰ-19) ( Ⅰ-23) 2.4 DiI-C 18 2min ( Ⅰ-25 26),,,,,, 5min, DiI-C 18, DiI-C 18 ( Ⅰ-27 28) 3 3.1,,,, W-G, Ⅰ, 2 3 Ⅰ Ⅰ Ⅰ (Brachydanio rerio) [11] [12],,, Ⅰ Ⅱ [12], Ⅰ Ⅱ [11], Ⅲ,, (PAS ACP) Ⅰ, Ⅰ, 3.2 (Cyprinus carpio) [15] (Siniperca chuatsi) [16] (Carassius auratus cuvieri) [17],,,,, DiI-C 18 DiI-C 18 [18], DiI-C 18, 12% [19],,,, DiI-C 18, DiI-C 18,, DiI-C 18 [5],, [20], [21,22],, ( GFPs [23] ) DiI-C 18 (2min),,, cdna 3.3 (POX), H 2 O 2 H 2 O 2, [12] POX (Ctenopharyngodon idellus) [24]

5 [24] POX,, POX,, (ACP), [25] ACP, ACP, (Oreochromis niloticus) [1], ACP, [1] [3] ACP, ACP [3] (Ictalurus punctatus) ACP [24,26], ACP, AKP, ACP ACP -α- (ANAE) T,,,,, [24], : [1] Witten P E, Villwock W, Renwrantz L. Haematogram of the tilapia Oreochromis niloticus (Cichlidae, Teleostei) and application of a putative phenoloxidase for differentiating between neutrophilic granulocytes and monocytes [J]. Canadian Journal of Zoology, 1998, 76: [2] Zhang X L, Wang M Q, Jiang L Z. Peripheral blood cells morphology and histochemical reaction of adult Silurus meridionalis [J]. Sichuan Journal of Zoology, 1998, 17(3): [,,., 1998, 17(3): ] [3] Zhao B S, Shen C G. A morphological and cytochemical study of the peripheral blood leucocytes on five species of the juvenile in cyprinidae [J]. Acta Hydrobiologica Sinica, 1991, 15(2): [,.., 1991, 15(2): ] [4] Patton E E, Zon L I. The art and design of genetic screens: zebrafish [J]. Nature Reviews Genetics, 2001, 2: [5] Gousset K, Wolkers W F, Tsvetkova N M, et al. Evidence for a physiological role for membrane rafts in human platelets [J]. Journal of Cell Physiology, 2002, 190: [6] Houston A H, Murad A. Erythrodynamics in fish: recovery of the goldfish Carassius auratus from acute anemia [J]. Canadian Journal of Zoology, 1995, 73: [7] Duncan P L, Lovell R T, Butterworth Jr C E, et al. Dietary folate requirement determined for channel catfish, Ictalurus punctatus [J]. Journal of Nutrition, 1993, 123: [8] Noyes A D, Grizzle J, Mand Plumb J A. Hematology and histopathology of an idiopathic anemia of channel catfish [J]. Journal of Aquatic Animal Health, 1991, 3: [9] Murad A, Everill S, Houston A H. Division of goldfish erythrocytes in circulation [J]. Canadian Journal of Zoology, 1993, 71: [10] Ellis A E. Bizarre forms of erythrocytes in a specimen of plaice, Pleuronectes platessa L. [J]. Journal of Fish Disease, 1984, 7: [11] Lieschke G J, Oates A C, Crowhurst M O, et al. Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish [J]. Blood, 2001, 98(10): [12] Cheng L Z, Zhong C P, Cai W Q. Contemporary Histology [M]. Shanghai: Shanghai Scientific and Technological Literature Publishing House. 2003, [,,.. :. 2003, ] [13] Rowley A F, Hunt C, Page M, et al. Fish, in Vertebrate blood cells, Rowley A F and Ratcliffe N A, Editors. Cambridge University Press: Cambridge. 1988, [14] Fijan N. Morphogenesis of blood cell lineages in channel catfish [J]. Journal of Fish Biology, 2002, 60: [15] Feng H L, Li W W, Wang T H, et al. Observation on the micromorphological and super-micromorphological structures of the blood cell of carp [J]. Journal of Fisheries of China, 1991, 15(3): [,,,.., 1991, 15(3): ] [16] Yuan S Q, Zhang Y A, Yao W J, et al. Micro and ultrastructure of peripheral blood cells of the Mandarin fish, Siniperca chuatsi (Basilewsky) [J]. Acta Hyrobiologica Sinica, 1998, 22(1): [,,,.., 1998, 22(1): 39 47] [17] Xie Y X, Lin G H. Studies on the microstructure and ultrastructure of peripheral blood cell of Carassius auratus cuvieri [J]. Chinese Journal of Zoology, 1996, 31(1): [,.., 1996, 31(1): 12 16] [18] Honig M G, Hume R I. DiI and DiO: Versatile fluorescent dyes for neuronal labeling and pathway tracing [J]. Trends Neurosci, 1989, 12: [19] Douste-Blazy L, Chap H, Gautheron P. Platelet lipid composition [J]. Haemostasis, 1973, 2: [20] Fantini J, Garmy N, Mahfoud R, et al. Lipid rafts: structure, function and role in HIV, Alzheimer s and prion diseases [J] : DOI: /S [21] Brown D A. Lipid raft, detergent-resistant membranes, and raft targeting signals [J]. Physiology, 2006, 21: [22] Shrimpton C N, Borthakur G, Larrucea S, et al. Localization

6 3 : 555 of the Adhesion Receptor Glycoprotein Ib-IX-V Complex to Lipid Rafts Is Required for Platelet Adhesion and Activation [J]. Journal of J experiment Med, 2002, 196(8): [23] Zacharias D A, Violin J D, Newton A C, et al. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells [J]. Science, 2002, 296: [24] Guo Q L, Lu Q Z. Ultrastructure and cytochemical studies on the development of haemetic cells in the kidney and spleen of the grasscarp [J]. Acta Hydrobiologica Sinica, 1994, 18(3): [,.., 1994, 18(3): ] [25] Vázquez G R, Guerrero G A. Characterization of blood cells and hematological parameters in Cichlasoma dimerus (Teleostei, Perciformes) [J]. Tissue and Cell, 2007, 39: [26] Petrie-Hanson L, Ainsworth A J. Differential cytochemical staining characteristics of channel catfish leukocytes identify cell populations in lymphoid organs [J]. Veterinary Immunology and Immunopathology, 2000, 73(2): [27] Bainton D F. Morphology of neutrophils, eosinophils, and basophils, in Williams Hematology, Beutler E, Coller B, Lichtman M A, et al, Editors. McGraw-Hill: Sydney. 2001, Ⅰ PlateⅠ ; (PAS) ; ; (ANAE) ; ; ; 24 ; DiI-C 18 ; DiI-C 18 ; 1 24 = 10 µm; = 20 µm 1 7. Giemsa and Wright staining; Periodic Acid Schiff reaction; acid phosphatase (ACP) staining; α-naphthyl acetate esterase (ANAE) staining. 24, 26 and 28 were taken by phase contrast microscopy; 25 and 27 were taken by fluorescent microscopy. 24 show live granulocytes; 25 and 26 show DiI-C 18 labeled thrombocytes; 27 and 28 show DiI-C 18 labeled thrombocytes aggregation E: ; DE: ; IE: ; OE: ; G-I:Ⅰ; G-II: Ⅱ; G-Ⅲ: Ⅲ; M: ; LL: ; SL: ; L: ; T: E: erythrocyte; DE: dividing erythrocyte; IE: immature erythrocyte; OE: old erythrocyte; G-I: granulocyte-ii; G-II: granulocyte-Ⅱ; G-Ⅲ: granulocyte-Ⅲ; M: monocyte; LL: large lymphocyte; SL: small lymphocyte; L: lymphocyte; T: thrombocyte

7 Ⅱ PlateⅡ 1., ; 2. Ⅰ; 3. Ⅰ ; 4. Ⅰ ; 5. Ⅰ Ⅱ ; 6. Ⅱ; 7. ; 8. ; 9. ; 10. ; 11. ; showing mitochondrium in erythrocyte; 2. granulocyte -I; 3. showing endoplasmic reticulum in granulocyte -I; 4. Showing golgi complex in granulocyte -I; 5. Showing granular -II in granulocyte -I; 6. granulocyte -II; 7. monocyte; 8. Showing lysosome-like granular in monocyte; 9. lymphocyte; 10. Showing free-ribosomes in lymphocyte; 11. thrombocyte; 12. showing golgi complex in thrombocyte Mt: ; N: ; HC: ; Gc: ;Ⅰ:Ⅰ ; Ⅱ: Ⅱ ; RER: ; SER: ; BG: ; LG: ; P: ; V: ; R: ; CV: ; LCV: ; Ocv: Mt: mitochondrium; N: nucleus; HC: heterogenous chromatin; Gc: golgi complex; I: granular-i; II: granular-ii; RER: rough endoplasmic reticulum; SER: smooth endoplasmic reticulum; BG: large bubble-like granular; LG: lysosome-like granular; P: pseudopod; V: vesicle; R: free-ribosome; CV: cannular vesicle; LCV: large cannular vesicle; Ocv: an vesicle opening on cell surface

Microsoft Word 曹伏君_new_.doc

Microsoft Word 曹伏君_new_.doc 45 2 Vol.45, No.2 2014 3 OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2014 (Lethrinus erythropterus) * ( 524025) Wright (Lethrinus erythropterus),,,, ; ; ; Q954.56 doi: 10.11693/hyhz20140100011 (Lethrinus erythropterus)