Microsoft Word - 18 黄翔峰.doc

Size: px

Start display at page:

Download "Microsoft Word - 18 黄翔峰.doc"

缄甄宰
5 years ago
Views:

1 微生物学通报 NOV 20, 2009, 36(11): 1738~1743 Microbiology 专论与综述 2009 by Institute of Microbiology, CAS 废弃食用油脂生物合成鼠李糖脂研究进展 * 黄翔峰陈旭远刘佳陆丽君 ( ) 摘要 : 碳源的成本过高限制了鼠李糖脂的工业化生产及应用, 废弃食用油脂作为一种廉价易得的碳源, 越来越多的研究者开始关注用它发酵生产鼠李糖脂废弃食用油脂的种类投加量对鼠李糖脂的产量结构性质均会产生影响, 目前研究中用废弃食用油脂作碳源, 鼠李糖脂产量最高可达 g/l 表面张力最低达到 24 mn/m 产物 CMC 最低可达 mg/l 此外, 本文还总结了菌株氮源微量元素 ph 溶氧及培养方式等因素对废弃食用油脂生产鼠李糖脂的影响, 并展望了利用废弃食用油脂生产鼠李糖脂实现产业化的重点研究方向关键词 : 鼠李糖脂, 废弃食用油脂, 脂肪酸, 生物表面活性剂 Recent Progress on Rhamnolipid Produced from Fermentation of Waste Edible Oils HUANG Xiang-Feng * CHEN Xu-Yuan LIU Jia LU Li-Jun (College of Environmental Science & Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai , China) Abstract: Although it is widely studied as a promising bio-surfactant, biosynthesis of Rhamnolipid has not been applied in large-scale due to its high production cost. As a cheap alternative carbon source, waste edible oils have been extensively studied for the production of rhamnolipid. This paper reviewed the recent research in this field, including the influence of various waste edible oils and production, chemical structure and properties of the produced rhamnolipids. With waste edible oils, the maximum production of rhamnolipids was reported to be g/l. The lowest surface tension was 24 mn/m and the lowest CMC of the produced rhamnolipids was mg/l. In addition, this paper also summarized the effect of various factors on the rhamnolipids biosynthesis, such as bacteria strains, nitrogen sources, trace minerals, dissolved oxygen, ph and fermentation conditions. Based on this, the key points of the mass production of rhamnolipids with waste edible oils were also discussed. Keywords: Rhamnolipid, Waste edible oils, Fatty acids, Biosurfactant 基金项目 : (No ); (No. 06DZ22007); (No ) * 通讯作者 :Tel: ; : hxf@tongji.edu.cn 收稿日期 : ; 接受日期 :

2 : 1739 (Biosurfactant),,,,,, [1] 1~2 ( R), 1~2 C 8 ~C 12 ( L), 4, RL RL 2 R 2 L R 2 L 2 (Pseudomonas spp.), (Pseudomonas aeruginosa), (Pseudomonas putida), P. aeruginosa AT10 [2], P. aeruginosa LBI [3], 50% [4],, [5], [6],,,, [7,8], (Waste edible oils, WEOs) [9] Dietzia sp. S-JS-1, [10], 1 废弃食用油脂 [11], 2005~2006, 4,, 1.1 废弃食用油脂的分类 3,,, (C 18:1 )(C 18:2 ), Kock [12] 30%,, [13,14] (Soapstock),, 2%~3%, [15],, (C 16 ) (C 18:3 ) [15,16],, 60% [17],,, 1.2 废弃食用油脂作碳源的预处理,, [18 22],,,, Zhu [19]

3 1740 微生物学通报 2009, Vol.36, No h,, Raza [20,23,24], 2 废弃食用油脂对生产鼠李糖脂的影响,, CMC 4 1, (24.61 g/l);, (24 mn/m); CMC (40.19 mg/l), Nitschke [22], 4.09 g/l, 3.9 mn/m,,, 2, [9,23] 2.1 WEOs 对鼠李糖脂产量的影响, ( 1), P. aeruginosa LBI, Table 1 表 1 不同废弃食用油脂条件下鼠李糖脂的产量及性质 Production and surface properties of rhamnolipid produced with various waste edible oils Carbon sources Rhamnolipids production (g/l) ST (mn/m) Rhamnolipids properties IFT CMC (mn/m) (mg/l) EI (E 24, %) References Used olive oil 32 60(Kerosene) [18] Used sunflower oil (Kerosene) [18] Waste frying oil from the public catering [19] Waste soybean oil* [20] Used soybean oil [22] Waste soybean oil <1(n-Hexadecane) (n-Hexadecane) [24] Waste frying oil (olive/sunflower, 1:1) (Kerosene) [25] Soybean soapstock [2] Soapstock (Hexadecane) 120 [3] Soapstock and the acidic wastewater from sunflower oil production (Kerosene) (Almond oil) [15] Sunflower soapstock [21] Soybean soapstock (Hexadecane) (Kerosene) [22] Corn soapstock (Hexadecane) (Kerosene) [22] Palm soapstock (Hexadecane) (Kerosene) [22] Vegetable oil refinery (n-Hexadecane) [23] Oil wastewater (0# diesel) [26] OOME (Kerosene) 75(Kerosene) [27] : *:. Note: *: The strain in this study is P. putida, and the strain used in the rest studies are all P. aeruginosa.

4 : g/l,, 11.7 g/l g/l 8.65 g/l 8.62 g/l [22] ;, P. aeruginosa EBN-8, 8.50 g/l [23] Benincasa [21],, 12 g/l, 7.1 g/l,, 5.4 g/l 4.8 g/l,, 40 g/l Zhu [19] 1% 5%(V/V) 5, 4% ( 35 g/l), Abalos [28] 50 g/l, 2.2 WEOs 对鼠李糖脂结构的影响,, RC 10 C 10 R 2 C 10 C 10 Benincasa [3] 6, R 2 C 10 C 10 R 1 C 10 C 10 52%; Haba [25] 1:1, 11, R 2 C 10 C 10 RC 10 C 10 53%; Abalos [2] 7, R 2 C 10 C 10 RC 10 C 10 85%,, 50%, 30.9%(R 2 C 10 C 12:1 + RC 10 C 12:1 ) [15],,, [29] 2.3 WEOs 对鼠李糖脂性质的影响 CMC [2,25] Raza [24], 29.1 mn/m Haba [18], 36,, 32 mn/m~36.5 mn/m, 55%~87% Nitschke [22],,, CMC, mg/l 2.4 WEOs 的利用情况 Abalos [2], 21.1%48.4% 6.23%,, 9% 9.10% 3.21% Benincasa [3,21], P. aeruginosa LBI, 25%50% 7% 4%, 48 h, 4% 0.12%, 1%, Nitschke [22],, 82.20% 100%, 60.23%52.47% 74.2%,,,, [3] 3 其他条件对生产鼠李糖脂的影响 3.1 菌株突变, Zhu [19],

5 1742 微生物学通报 2009, Vol.36, No.11, g/l g/l 3.2 氮源, [30] C/N Benincasa [15] C/N, C/N 8, 48 h, 7.3 g/l Haba [18] C/N , C/N 8,, 0.34 Raza [23] C/N, C/N 20, Gloria [31], C/N Mercade [27] NaNO 3, 1 g/l 2.5 g/l 4 g/l 6 g/l, 2.5 g/l,,,, 3.3 微量元素,, Zn Cu Mn B Mo 5, Zhu [19] Co Fe(3 ) 2, g/l,, Co Fe,, Fe [32] 3.4 ph 与溶氧条件 ph 5~9, ph 7, ph , 6.8 Zhu [19] 0.67 mol/l H 3 PO 4 2 mol/l KOH ph 6.8; Benincasa [21] 2 mol/l HCl 2 mol/l NaOH ph 6.8, Benincasa [21] K La, h h 1,, 3.5 培养方式 Raza [24] 3,,, Benincasa [21], 12 g/l, 15.9 g/l 4 展望,,,, : 1),, 2) 3), 4) ph,,, 参考文献 [1] Ochoa-Loza FJ, Artiola JF, Maier RM. Stability constants for the complexation of various metals with a rhamnolipid biosurfactant. Journal of Environmental Quality, 2001, 30(2): [2] Abalos A, Pinazo A, Infante MR, et al. Physicochemical

6 : 1743 and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes. Langmuir, 2001, 17(5): [3] Benincasa M, Abalos A, Oliveira I, et al. Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2004, 85(1): 1 8. [4] Thavasi R, Jayalakshmi S, Balasubramanian T, et al. Biosurfactant production by Corynebacterium kutscheri from waste motor lubricant oil and peanut oil cake. Letters in Applied Microbiology, 2007, 45(6): [5] Das P, Mukherjee S, Sen R. Substrate dependent production of extracellular biosurfactant by a marine bacterium. Bioresource Technology, 2009, 100(2): [6],,.., 2007, 34(1): [7] Maier RM, Soberon-Chavez G. Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Applied Microbiology and Biotechnology, 2000, 54(5): [8],,,.., 2005, 15(3): [9] Makkar RS, Cameotra SS. Biosurfactant production by microorganisms on unconventional carbon sources. Journal of Surfactants and Detergents, 1999, 2(2): [10],,,. Dietzia sp. S-JS-1., 2009, 36(4): [11] Dumont MJ, Narine SS. Soapstock and deodorizer distillates from North American vegetable oils: review on their characterization, extraction and utilization. Food Research International, 2007, 40(8): [12] Kock JLF, Botha A, Bloch J, et al. Used cooking oil: science tackles a potential health hazard. South African Journal of Science, 1996, 92(11-12): [13] Melton SL, Jafar S, Sykes D, et al. Review of stability measurements for frying oils and fried food flavor. Journal of the American Oil Chemists Society, 1994, 71(12): [14] Tyagi VK, Vasishtha AK. Changes in the characteristics and composition of oils during deep-fat frying. Journal of the American Oil Chemists Society, 1996, 73(4): [15] Benincasa M, Accorsini FR. Pseudomonas aeruginosa LBI production as an integrated process using the wastes from sunflower-oil refining as a substrate. Bioresource Technology, 2008, 99(9): [16] Durant AA, Dumont MJ, Narine SS. In situ silylation for the multicomponent analysis of canola oil by-products by gas chromatography-mass spectrometry. Analytica Chimica Acta, 2006, 559(2): [17] Dowd M. Compositional characterization of cottonseed soapstocks. Journal of the American Oil Chemists' Society, 1996, 73(10): [18] Haba E, Espuny MJ, Busquets M, et al. Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB from waste frying oils. Journal of Applied Microbiology, 2000, 88(3): [19] Zhu Y, Gan JJ, Zhang GL, et al. Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1m. Journal of Zhejiang University-Science A, 2007, 8(9): [20] Raza ZA, Khan MS, Khalid ZM. Evaluation of distant carbon sources in biosurfactant production by a gamma ray-induced Pseudomonas putida mutant. Process Biochemistry, 2007, 42(4): [21] Benincasa M, Contiero J, Manresa MA, et al. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source. Journal of Food Engineering, 2002, 54(4): [22] Nitschke M, Costa S, Haddad R, et al. Oil wastes as unconventional substrates for rhamnolipid biosurfactant production by Pseudomonas aeruginosa LBI. Biotechnology Progress, 2005, 21(5): [23] Raza ZA, Rehman A, Khan MS, et al. Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes. Biodegradation, 2007, 18(1): [24] Raza Z, Khan M, Khalid Z, et al. Production kinetics and tensioactive characteristics of biosurfactant from a Pseudomonas aeruginosa mutant grown on waste frying oils. Biotechnology Letters, 2006, 28(20): [25] Haba E, Pinazo A, Jauregui O, et al. Physicochemical characterization and antimicrobial properties of rhamnolipids produced by Pseudomonas aeruginosa 47T2 NCBIM Biotechnology and Bioengineering, 2003, 81(3): [26],,,. O-2-2., 2005, 25(z1): [27] Mercade ME, Manresn MA, Robert M, et al. Olive oil mill effluent (OOME). New substrate for biosurfactant production. Bioresource Technology, 1993, 43(1): 1 6. [28] Abalos A, Maximo F, Manresa MA, et al. Utilization of response surface methodology to optimize the culture media for the production of rhamnolipids by Pseudomonas aeruginosa AT10. Journal of Chemical Technology and Biotechnology, 2002, 77(7): [29] Pornsunthorntawee O, Wongpanit P, Chavadej S, et al. Structural and physicochemical characterization of crude biosurfactant produced by Pseudomonas aeruginosa SP4 isolated from petroleum-contaminated soil. Bioresource Technology, 2008, 99(6): [30],,,.., 2003, 30(3): [31] Soberón-Chávez G, Lépine F, Déziel E. Production of rhamnolipids by Pseudomonas aeruginosa. Applied Microbiology and Biotechnology, 2005, 68(6): [32] Ochsner UA, Hembach T, Fiechter A. Production of rhamnolipid biosurfactants. In: Brázana E, Freitag R, Horváth C, et al. Downstream Processing Biosurfactants Carotenoids (Advances in Biochemical Engineering / Biotechnology 53). Berlin: Springer-Verlag, 1996, pp

: 307, [], [2],,,, [3] (Response Surface Methodology, RSA),,, [4,5] Design-Expert 6.0,,,, [6] VPJ33 ph 3,, ph, OD, Design-Expert 6.0 Box-Behnken, VPJ3

: 307, [], [2],,,, [3] (Response Surface Methodology, RSA),,, [4,5] Design-Expert 6.0,,,, [6] VPJ33 ph 3,, ph, OD, Design-Expert 6.0 Box-Behnken, VPJ3 微生物学通报 FEB 20, 2008, 35(2) 306~30 Microbiology 2008 by Institute of Microbiology, CAS tongbao@im.ac.cn 生物实验室响应面分析法优化副溶血性弧菌生长条件刘代新宁喜斌 * 张继伦 2 (. 200090) (2. 20202) 摘要