892311B009006

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扶弗萧
4 years ago
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2 has been noticed as functional food. This research is to investigate the influence of different environmental factors on the production of mycelium and polysaccharide in submerged culture of C. sinensis. The optimal medium condition for the mycelium and polysaccharide production is initial ph=6, glucose 5% and yeast extract 1%. The best carbon and nitrogen source are glucose and yeast extract, respectively. Adding NH 4 H 2 PO 4 is to inhibit the yield of polysaccharide. The optimal concentration of the MgSO 4 7H 2 O and olive oil to added is 5.6% and.12%. However, we study these 1 yeast extract.5 yeast extract + three factors for polysaccharide formation by.5% con steep liquor ph 5-7 RSM, we found the interaction of factors had NH 4 H 2 PO 4 significant on inhibition of polysaccharide formation Keywords: Cordyceps sinensis, polysaccharide, Response Surface Methodology Abstract Dongchongxiachao is famous as crude drug in Chinese medicine expensively and rarely. The therapy and health claims include the benefits to lung and kidney, the enforcement of immunity and antitumor activity. The yield of Dongchongxiachao is limiting by the geographyc region and the condition of climate in the mainland of China leading to dear the price. The great potency of application in C. sinensis by fermentation 1~4 15~ 25 2~8 2~4(~6) 1

3 1 (1). (2) (3) (4). (5). 3 4 D- 5 6 Kiho 45 kda SE-HPLC beta-1,3 7 (1). 118 PDB B A rpm.81g/ml B ph 5~ ph=6 C D rpm ph=6 Glucose 5 Yeast 1 extract 1 one-factor at (2). one time Guihua Guoshan 17 C. militaris 1951 Box Wilson 14 Surface A B Response Methodology RSM data fitting technique (3). peptone corn steep liquor 2

4 yeast extract yeast extract A B yeast Y= X X 2-1.4X 3 extract yeast extract.5 + corn steep liquor.5 yeast extract NH 4 H 2 PO 4 X 1 1 X 2 X 3 corn steep liquor yeast NH 4 H 2 PO 4 X 1 extract X 2 X 3 NH 4 H 2 PO 4 (4) Acremonium persicinum NH 4 H 2 PO 4 -glucan (Glucan) (Olive oil) (Sunflower oil) 18 (.4kgm -3 ) 12~14 kgm -3 22~24 kgm -3 Olive oil 28 kgm -3 C/N ph beta-glucan 19 A ~ D Olive oil C/N ph A NH 4 H 2 PO 4.1 C/N Olive oil.35.55ml B (NH 4 ) 2 HPO 4 ph Data not show C.1 D NH 4 H 2 PO 4 RSM (5). RSM 3

5 (1) (11) (12).Zhu., J.S., Halpern, G.M., and Jones, K. (1) a, The scientific rediscovery of the Chinese herbal medicine Cordyceps sinensis part I. J. Altern. Complement. (2) Med (13).Zhu., J.S., Halpern, G.M., and Jones, K b, The scientific rediscovery of a (3). precious ancient Chinese herbal 1991 regimen Cordyceps sinensis part II. J Altern. Complement. Med (4) (14).Box, G.E.P., and Wilson, K.B. 1951, On the experimental attainment optimum conditions. J. Roy. Statist. Soc. B (5). Miyazaki, T., Oilawa,N., and Yamada, (15) H.1997, Studies on fungal polysaccharides. XX. Galactomannan of Cordyceps sinensis. Chem. Pharm. Bull. (16) (6). Kiho, T., Hui, J.I., Yamane, A., and Ukai, S. 1993, Polysaccharides in fungi. (17).Guiha, Y., and Guoshan, W. 1988, XXXII. Hypoglycemic activity and Apreliminary report on growth habit of Chemical properties of the Cordyceps militaris. Edible Fungi. 4 polysaccharide from the culture mycelium of Cordyceps sinensis. Biol. Pharm. Bull (18).Stasinopoulos, S. J., and R. J. Seviour Stimulation of exopolysaccharide (7) production in the fungus Acremonium SE-HPLC persicinum with fatty acids. Biotech. Bioeng., beta (8). Kiho, T., Yamane, A., Hui, J.I., Usui, S., and Ukai, S. 1996, Polysaccharides in fungi. XXXVI. Hypoglycemic activity of the polysaccharide CS-3 from the culture mycelium of Cordyceps sinensis and its effects on glucose metabolism in mouse liver. Biol. Pharm. Bull U., and Wagner, F A high affinity Zn 2+ uptake system controls growth and (9). biosynthsis of an extracellular,branched ,3-B-1,6-glucan in Sclerotium 97 rolfsii ATCC 155. xp. Mycol., 15, (19).Reeslev, M., and Jensen, B Influence of Zn 2+ an Fe 3+ on polysaccharide production and mycelium/yeast dimorphism of Aureobasidium pullulans in batch cultivations. Appl. Microbiol. Biotechnol., ().Pilz, F., Auling, G., Stephan, D., Rau,

6 .82 A. B.. (g/ml) (mg/ml) (mg/ml) (day) ph 1 C. 1 D (mg/ml) (mg/ml) (mg/ml) 1 (mg/ml) Glucose ( % ) Yeast extract ( % ) A PDB 23 1 rpm B ph C D

7 A. (mg/ ml) 15 5 (mg/ml) Glucose 5% Sucrose % Glucose 2.5% Sucrose 2.5% Glucose % Sucrose 5% B. (mg/ml) (mg/ml) Glucose 5% Maltose % Glucose 2.5% Maltose 2.5% Glucose % Maltose 5% A B 6

8 1 A. (mg/ml) (mg/ml) yeast extract 1% peptone % yeast extract.5% peptone.5% yeast extract % peptone 1% B. (mg/ml) (mg/ml) yeast extract 1% corn steep liquor % yeast extract.5% corn steep liquor.5% yeast extract % corn steep liquor 1% yeast extract peptone A yeast extract corn steep liquor B 7

9 A B. 1 1 (mg/ml) 15 5 (mg/ml) (mg/ml) (mg/ml) Olive oil (%) NH 4 H 2 PO 4 (%) (mg/ml) 1 C. 1 1 (mg/ml) (mg/ml) 1 D. 1 1 (mg/ml) (NH 4 ) 2 HPO 4 (%) MgSO 4. 7H2 O (%) A Olive oil B NH 4 H 2 PO 4 C (NH 4 ) 2 HPO 4 D MgSO 4 7H 2 O 8

10 RSM NH 4 H 2 PO 4 MgSO 4 7H 2 O Oliver oil Polysaccharide No. (g/ ml) (g/ ml) (ml/ ml) (mg/ ml) (C) (C) No. 9 (C) 1(C) 9

11 NH 4 H 2 PO 4 MgSO 4 7H 2 O Oliver oil Polysaccharide No. (g/ ml) (g/ ml) (ml/ ml) (mg/ ml)

12 MgSO 4 7H 2 O Oliver oil Polysaccharide No. (g/ ml) (ml/ ml) (mg/ ml)

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