, 10, (28.98±2.69) cm, 1.2 [5], (foregut) (midgut) (hindgut)3, 5,, 5 = /, = / 100% 5,,,, 1.3,, 5, Bouin s 1 d,, 5 μm,, (HE), Olymp

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1 2011 9, 18(5): Journal of Fishery Sciences of China 研究论文 DOI: /SP.J 梁志强 1, 李传武 1, 刘明求 1, 卞伟 1, 余长生 2, 杨奠元 1.,, ; 2.,, 摘要 : HE, 10 (28.98±2.69) cm [Sinilabeo decorus tungting (Nichols)],,, : (1), 3, ;,, 21.42±3.51, 42±5 (2), (3), Z,, (4) 4 (P<0.01), (P<0.05),, (5),,,, (6), : ; ; ; ; 中图分类号 : S94 文献标志码 : A 文章编号 : (2011) [Sinilabeo decorus tungting (Nichols)], 鲃 (Barbinae), (Sinilabeo),, 1980,, [1], 2000,, t,, [2] 2007,, [2] [3],, (Ctenopharyngodon idellus) (Hypophthalmichthys molitrix) (Aristichthys nobilis)(hucho taimen) (Cheilinus undulates) (Pampus argenteus)(tilapia nilotica) (Himantura signifer) (Leporinus friderici) [4 11] [12 16], Spot321,,, 收稿日期 : ; 修订日期 : 基金项目 : (2006NK3128); (XMY200608, XMY200710, XMY200806); ([2008]155 ). 作者简介 : (1977 ),,,,. liangzhiqiang@163.com

2 , 10, (28.98±2.69) cm, 1.2 [5], (foregut) (midgut) (hindgut)3, 5,, 5 = /, = / 100% 5,,,, 1.3,, 5, Bouin s 1 d,, 5 μm,, (HE), Olympus BX51, 1.4 Spot321, 3, 3 ( ), 45 (5 3 / 3 / ), 1.5 SPSS11.5, ± ( x ± SD) t P<0.05 ; P< ,,,,, (oropharyngeal cavity) (Oesophagus)(intestine)3 ( 1); (hepatopancreas) ( 1 1), ( 2),,,,,,, / 1.09±0.13( 1), ( 3) 5,,,,,, , 5,, ( 4), 5, 5 4 2/2 4 5, / / / / ,, ( 5);,,, ( 6), 1/50 ( 1 6),,,, 2, 21.42±3.51,

3 5 : 1053 Tab.1 character / mouth cleft length/snout length / oral cavity length/head length / oral cavity volume/head volume spiral number of gut ( )/( ) ratio of oral cavity volume of open mouth to closed mouth /% hepatopancreas weight/total weight 表 1 湘华鲮消化系统参数 Character of digestive system of Sinilabeo decorus tungting value 1.09± ± ± ±5 2.60± ±0.15 character intestine length/body length /mm middle oesophagus diameter /mm middle forgut diameter /mm middle midgut diameter /mm middle hindgut diameter type of pharyngeal teeth n=10; x ±SD value 21.42± ± ± ± ± ±5,,,, ( 7 10),, 1/3, ; 1/3 Z ; Z ( 8 11), ;,, ( 9) 2.1.3,, 1.21± (mucosa layer) (submucosa layer) (muscle) (stratified epithelial tissue), (taste bud) (mucus-secreting cell),, ( 1),, ( 2), ( 3),,,, ( 3, 3),,, ( 3) 4 (18 22, 4), ( 5),,,,,,,, 1,,,, ( 6 1),,, Z, ( 7 8 9, 1),,, ( 7)

4 ,,,,, ( 6 7, ),, ( 8, 4 5),, ( 8) ; ( 8),,, ( 7),,,, ( 8) (44±6), (P<0.01); (P<0.01);, 8±2 ( 2) ( μm), (P<0.05), (P<0.01); ( μm),, [(313.96±151.01) μm], (P<0.01); [(44.88± 12.25) μm], (P<0.01) (P<0.05),, [(36.08±6.48) μm], [(9.89±2.14) μm],, 6, (P<0.05)(P<0.01), 4 : ( 2) (P<0.05)(P<0.01), 2, (mucussecreting cell)(goblet cell) [5],,,,, ;,,, ( 3) Tab.2 表 2 湘华鲮消化道组织形态指数 Morphological indices of digestive tract of Sinilabeo decorus tungting n=10; x ±SD morphological index esophagus foregut midgut hindgut plica mucosa number 20±2 c 44±6 a 10±3 e 8±2 e /μm plica mucosa height ± b ± a ± d ± d /μm submucosa thickness ±22.95 a 21.59±6.76 f 84.95±15.79 b 40.35±11.25 d /μm circular muscle thickness ± a 81.25±18.62 c 44.88±12.25 e 69.61±12.80 c /μm longitudinal muscle thickness 3.68±0.77 d 32.73±20.97 a 2.41±0.90 e 9.18±3.71 b /μm serosa thickness 21.36±12.18 b 16.70±8.16 bc 9.89±2.14 c 36.08±6.48 a : (P>0.05); (P<0.05); (P<0.01). Note: The same superscript in the same row donate nonsignificant difference (P>0.05). Values with neighboring superscripts in the same row donate significant difference (P<0.05). Values with apart superscripts in the same row donate extremely significant difference (P<0.01).

5 5 : 1055,,,, ( 7),,,,,, (2 937±475) /mm 2 ( 3, 5),, (145±144) /mm 2, (P<0.01), (P<0.05),, (1 650±400) /mm 2,, ;, 3 3.1, (Achnanthes)(Surirella) (Synedra),, [12] [17],,,,, [4] ( ) ( ) 鳡 ( )()( ) () [17], 1; 1~3 ; 3, 15~20 [17], 21.42± 3.51, (1.64~2.45) (2.29~2.54) (0.54~0.63)(4.63~4.97)(6.29~7.77) [4] (Xenocypris davidi)(6.26) [18], 42±5, (6 ) (8 ) (0 )(24 ) [4], [19] Z, [17],,,,, Tab.3 表 3 湘华鲮消化道黏膜皱褶中黏液细胞的数目 Numbers of mucous cells on digestive tract of Sinilabeo decorus tungting n=10; x ± SD; cells mm 2 mucus-secreting cells goblet cell position prepharynx upper plate esophagus foregut midgut hindgut head 1188±196 a 788±304 c 725±243 c 538±288 ce 338±151 e middle 2100±576 a 1550±355 b 1650±400 b 463±151 d 650±359 d base 1925±772 c 2937±475 a 237±141 e 713±352 g 688±304 g : (P>0.05); (P<0.05); (P<0.01). Note: The same superscript in the same row donate nonsignificant difference (P>0.05). Values with neighboring superscripts in the same row donate significant difference (P<0.05). Values with apart superscripts in the same row donate extremely significant difference (P<0.01).

6 ,,,, [4], [5] 3, [5], 3,,,,,,,,,,,,,,,,,, [20],,,,, [21] (Silurus meridionalis),,,, [22],,,,,, 参考文献 : [1]. [M]. :, 1976: [2],,,. [J]., 2010, 3(5): [3]. [M]. 2. :, 1998: [4]. [J]., 1962(3): [5],. [J]., 1963, 3: [6],,,. [J]., 2008, 15(5): [7],, 珺,. [J]., 2010, 34(4): [8],,. [J]., 2007, 14 (4): [9] Morrison C M, Wright J R. A study of the histology of the digestive tract of the Nile tilapia [J]. J Fish Biol, 1999, 54: [10] Kannika C, Ricardo M, Jintana P, et al. Histology of the digestive tract of the freshwater stingray Himantura signifer Compagno and Roberts, 1982 (Elasmobranchii, Dasyatidae) [J]. Anat Embryol, 2006, 211(5): [11] Albrecht M P, Ferreira M F, Caramaschi E P. Anatomicalfeatures and histology of the digestive tract of two relatedvneotropical omnivorous fishes (Characifomes; Anostomidae) [J]. J Fish Biol, 2001, 58: [12],,. [J]., 1982(4): [13]. [J]., 1966(5): [14]. ()[M]. :, 1977:

7 5 : 1057 [15],,. [J]., 1982(4): [16],,,. [J]., 2009, 31(4): [17]. [M]. :, 1995: [18],,. Xenocypris davidi Bleeker [J]., 1996,15(3): [19],,. [M].:, 1987: [20],,,. [J].,1997 (3): [21],. [J]., 2002(1):6 8. [22] Humbert W, Kirsch R, Meister M F. Scanning electron microscopic study of the oesophageal mucous Layer in the eel, Anguilla anguilla[j]. Fish Biol, 1984, 25: Morphological and histological observation of the digestive system in Sinilabeo decorus tungting (Nichols) LIANG Zhiqiang 1, LI Chuanwu 1, Liu Mingqiu 1, BIAN Wei 1, YU Changsheng 2, YANG Dianyuan 2 1. Fisheries Research Institute of Hunan Province, Changsha , China; 2. Animal Husbandry and Aquatic Products Bureau of Huaihua City, Huaihua , China Abstract: Sinilabeo decorus tungting (Nichols) is indigenous to Hunan province, China. This species is currently listed as endangered however little is known about its biology. To address this, we successfully propagated S. decorus tungting in captivity and used the offspring to document the morphology of the digestive system by light microscopy observation of tissue sections. The digestive system consisted of the digestive tract, which was composed of mouth, pharynx, esophagus, and intestine, and digestive gland. The specimens we examined had an inferior mouth, no stomach, 2 pairs of barbels, 5 types of pharyngeal teeth (typically 5 4 2/2 4 5), and lateral line scales. The intestine was extremely long and twisted 42±5 times. The ratio of intestine length to body length was 21.42±3.51, and that of hepatopancreas weight to total weight was 1.21±0.15. S. decorus tungting is a detritivore that scratches food with its lip. The walls of the intestine and esophagus consisted of mucosa, submucosa, muscular coat, and serosa layers. The mucosal layer of the prepharynx upper plate, esophagus, and intestine was covered with wrinkles. The thickness of each layer differed among the segments of the digestive system. The thickness of the submucosa differed significantly between the foregut, midgut, and hindgut (P<0.01). Similarly, the thickness of the longitudinal muscle differed between the foregut, midgut, and hindgut (P<0.05). The submucosa and circular muscle were thicker in the esophagus than in the foregut, midgut, and hindgut. Similarly, the mucosal and submucosal thickness was greater in the plica than in the hindgut. The foregut mucosa had 44±6 wrinkles, and the number of wrinkles decreased from the foregut to the hindgut. The density of mucous cells was highest in the mid-plica mucosa of the esophagus (2 100±576 cells/mm 2 ). The density of mucous cells was higher in the plica mucosa of the prepharynx than in the goblet cells of the intestine, and higher in the foregut than in the hindgut. The density of mucous cells differed between the plica mucosa in different regions of the gut and decreased from the base to the head of the plica mucosa in the esophagus and hindgut. Similarly, the density of mucous cells differed between the plica mucosa of the foregut, midgut, and hindgut. The density in the head of the plica mucosa tended to decrease between the foregut and hindgut. Food was absorbed in both the esophagus and the intestine. The morphology of the gut was consistent with that of other detritivores, being characterized by a long intestine, rich plica mucosa, and high density of mucous cells. Key words: Sinilabeo decorus tungting (Nichols); digestive system; morphology; histology; detritivores

1058 18 图版 Ⅰ 湘华鲮消化系统的形态学观察 1. ; 2.; 3.; 4.; 5. ; 6. ; 7. ; 8.; 9.; 10. ; 11.. A: ; CP: ; FG: ; G: ; GR: ; H: ; HG: ; I: ; M: ; MG: ; NOR: ; OE: ; OIS: -; ORC: ; PUP: ; PT: ; RGZ: Z ; RR: ; SN:.

8 图版 Ⅰ 湘华鲮消化系统的形态学观察 1. ; 2.; 3.; 4.; 5. ; 6. ; 7. ; 8.; 9.; 10. ; 11.. A: ; CP: ; FG: ; G: ; GR: ; H: ; HG: ; I: ; M: ; MG: ; NOR: ; OE: ; OIS: -; ORC: ; PUP: ; PT: ; RGZ: Z ; RR: ; SN:. PlateⅠ Morphological observations of digestive system in Sinilabeo decorus tungting 1. dissection of digestive system; 2. mouth position; 3. prepharynx upper plate; 4. pharyngeal teeth; 5. esophag-gastric junction; 6. digestive tract; 7. anterior segment foregut; 8. posterior segment foregut; 9. midgut; 10. anterior segment foregut interior; 11. posterior segment foregut interior A: anus; CP: convex papillate; FG: foregut; G: gill; GR: gill raker; H: hepatopancreas; HG: hindgut; I: intestine; M: mouth; MG: midgut; NOR: no obvious reductus; OE: oesophageal; OIS: oesophageal-intestine sphincter; ORC: oropharyngeal cavity; PUP: prepharynx upper plate; PT: pharyngeal teeth; RGZ: reductus group Z; RR: reticulate reductus; SN: snout.

9 5 : 1059 图版 Ⅱ 湘华鲮消化系统 ( 唇前肠 ) 组织学观察 1.; 2. ; 3.; 4.; 5.; 6.; 7. ; 8.. BB: ; BV: ; CE: ; Cm: ; CSm: ; FD: ; GC: ; IC: ; KC: ; L: ; LM: ; Lm: ; LP: ; M: ; MB: ; MM: ; MSC: ; OL: ; SE: ; Se: ; SM: ; Sm: ; TB:. PlateⅡ Histological observation of digestive system from lip to foregut in Sinilabeo decorus tungting 1. longitudinal section of outside upper lip; 2. longitudinal section of inside upper lip; 3. cross-section of prepharynx upper plate; 4. cross-section of oesophagus; 5 cross-section of mucosa base of oesophagus; 6. cross-section of foregut; 7. cross-section of spiral valve head of foregut; 8. cross-section of foregut wall. BB: brush border; BV: blood vessel; CE: columnar epithelium; Cm: circular muscle; CSm: cross-striated muscle; FD: fatty drop; GC: goblet cell; IC: inside circular muscularis; KC: keratinocyte; L: lumen; LM: lamina muscularis; Lm: longitudinal muscle LP: lamina propria; M: mucosa; MB: muscle bundle; MM: muscularis mucosae; MSC: mucus-secreting cell; OL: outside longitudinal muscularis; SE: stratified squamous eqithelium; Se: serosa; SM: submucosa; Sm: smooth muscle; TB: taste bud.

1060 18 图版 Ⅲ 湘华鲮消化系统 ( 前肠肛门 ) 组织学观察 1.; 2.; 3.; 4. ; 5.; 6. ; 7.; 8.

10 图版 Ⅲ 湘华鲮消化系统 ( 前肠肛门 ) 组织学观察 1.; 2.; 3.; 4. ; 5.; 6. ; 7.; 8. AC: ; BS: ; BV: ; Cm: ; FD: ; GC: ; GR: ; L: ; LC: ; Lm: ; LP: ; M: ; MSC: ; PC: ; RR: ; Se: ; Sm: ; VIH: PlateⅢ Histological observation of digestive system from foregut to anus in Sinilabeo decorus tungting 1. longitudinal section of foregut mucosa; 2. cross-section of midgut; 3 cross-section of midgut part; 4. longitudinal section of midgut wall; 5. cross-section of hindgut; 6. cross-section of anus; 7. longitudinal section of gill-rakers; 8. hepatopancreas AC: anus conduit; BS: blood sinusoid; BV: blood vessel; Cm: circular muscle; FD: fatty drop; GC: goblet cell; GR: gill-rakers; L: lumen; LC: Liver cell; Lm: longitudinal muscle; LP: lamina propria; M: mucosa; MSC: mucus-secreting cell; PC: pancreas cell; RR: reticulate reductus; Se: serosa; Sm: smooth muscle; VIH: venae interlobulares hepatic.

Microsoft Word - 13-区又君.doc

Microsoft Word - 13-区又君.doc 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 海洋生物学 doi:10.3969/j.issn.1009-5470.2012.06.013 2012 年第 31 卷第 6 期 : 83 89 http://www.jto.ac.cn 波纹唇鱼消化道的形态学和组织学区又君 1, 廖光勇 1,2, 李加儿