5 : 29 in the digestive system1 Key words: Rhabdosargus sarba; digestive system; anatomy; histology; histochem istry R habdosargus sarba, Spa ri2 dae,

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1 South China Fisheries Science Vol14, No15 Oct1, , 2, 1, 3 (11, ; 21, ; 31, ) : (Rhabdosargus sarba), HEAB2PAS,, ;, ;,, ; ; 810,, AB2PAS 4,,,,,, : ; ; ; ; : Q95416 : A : (2008) Histological and histochem ical study of digestive system of Rhabdosa rgus sa rba GOU Xiaowei 1, 2, OU Youjun 1, L IAO Rui 3 ( 11Guangdong O cean U niversity, Zhanjiang , China; 21 South China Sea Fisheries Research Institute, Chinese A cadem y of Fishery Sciences, Guangzhou , China; 31 Shanghai O cean U niversity, Shanghai , China) Abstract: The histological structure and types of mucous cells in the digestive tracts ( i1e1, tongue, esophagus, stomach and intes2 tines) of Rhabdosargus sarba were observed and analyzed by using HE and AB2PAS ( alcian blue and periodic acid schiff s reation) conbined staining1 Results of study in which slices were stained with HE and showed that R1sarba has a broad oropharyngeal cavity and different type of teeth1 The stomach is divided to three parts which are cardiac stomach, stomach caeca and pyloric stomach1 The cardi2 ac stomach has the most gastric p it and gastric gland while stomach caeca has the thickest wall1 There are abundant mucous membrane p leats in the whole intestine1 R1sarba has 8 to 10 pyloric caecas which structure is sim ilar to anterior intestine1 The dividing lines of liver lobules are dim1 There are p lenty of tubular tissue in hepatopancreas1 Pancreatic acini and pancreatic islets distribute around main veins, acinar cells being stained into navy blue are closely arranged1 According to AB2PAS, 4 types of the mucous cells in the diges2 tive tracts of R1sarba were observed: typewas red, typewas blue, type was purp le reddish, and typewas blue purp le1 On2 ly a small quantity of type and type mucous cellswere found occasionally in stomach1 Most of mucous cells in the other digestive tracts were of type and type 1 The density of the mucous cells in the intestine was related to the functions of the different segments : ; : : (863 ) (2006AA10A414) : ( ),,, E2mail: gouxiaowei2000@ yahoo1com1cn :, E2mail: ouyoujun@1261com

2 5 : 29 in the digestive system1 Key words: Rhabdosargus sarba; digestive system; anatomy; histology; histochem istry R habdosargus sarba, Spa ri2 dae,, [ 1 ], ;,, 1967,,,, [ 2 ], [ 3 ] [ 4 ] [ 5 ] [ 6 ] [ 7 ] [ 8 ], cm, g 24 h,, ( ) 10%, 5m, HE AB2PAS N ikon 15, SPSS, (1) 56, 6,,,, 34, 5 6, 2, ,, 1 A1 ; B1 ; 11 ; 21 ; 31; 41 ; 51 ; 61 ; 71 Fig11Horizontal section of oral2pharyngeal cavity of R1sarba A1 dorsal part; B1 ventral part; 11 incisor; 21 molar; 31 tongue; 41 gill paker; 51 branchial arch; 61 upper pharyngeal tooth; 71 lower pharyngeal tooth,,, 3 810,, 2, 3, /0148, 2, 2,, 211,, 2, (2)

3 30 4,, 21212, 3 4,,,,,,,,, 2 11 ; 21 ; 31 ; 41 ; 51 ; 61 ; 71 ; 81 ; 91 ; 101 Fig12Morphological figure of the digestive tract 41 pyloric stomach; 51 anterior intestine; 61 m id2intestine; of R1sarba 11 oesophagus; 21 cardiac stomach; 31 stomach caeca;,, 40,,,,, ( I - 2),,, 71 posterior intestine; 81 liver; 91 pyloric caeca; 101 gallbladder,, ( I - 3) , 4,, 17,, m, m,,,,,,,,, ( I - 4),,, [ 9 ],,,,,,,,,, ( I - 1),,, 3 Fig13The comparison on histological structure of cardiac stomach, fundic stomach, pyloric stomach of R1sarba All Rights Reserved. South China Fisheries Science

4 5 : 31 4 Fig14The comparison on histological structure of anterior intestine, m id2intestine, posterior intestine and pyloric caeca of R1sarba,,,,, 4, 21217,,, ( I - 5) 21214, 2030,,,,,,,,,,, ( I - 6), 21215,, 2530,, ( I - 9) ( I - 7 ),,,,,,,, ( I - 10) ,, 21311, AB 2PAS, [ 10 ],,,, 4,, ( I - 8) AB2PAS, PAS

5 32 4 ;, AB 2PAS, AB ;, AB 2PAS, PAS, AB ;, AB2PAS, AB, PAS,, ( II), (1) 1 ( ind /mm 2 ) (, ) Tab11The com par ison on den sity( ind /mm 2 ) of an ter ior in testine, m id2in testine, poster ior in testine(m ean SD, ra te) part anterior intestine m id2intestine type type type type % % % % % % % % posterior intestine % % % pyloric caeca % % % % 3 311,,,,,,,,,, /0148, 810,, ( C rom ileptes altivelis) (L ateolabrax japonicus) ( Pagrosom us m ajor) BUDD INGTON D IA2 ( S iniperca chuatsi ) ( M uraenesox cine2 reius) [ ],,, /1 [ 9, 16 ], (Hypophthalm ichthys m olitrix) 8 [ 17 ] [ 18 ] (C tenopharyn2 godon idella) 25, 6 [ 19 ] (C lupanodon puncta tus) (A losa reevesii) (Sard inella aurita), [ 21 ] , (V aricorhi2 nus m acrolepi) [ 22 ] /3104 [ 22 ],, , 10, 1013, [ 23 ] ( Paralich2 thys olivaceus) 8, 8, , 600, MOND [ 24 ],, [ 25 ], [ 26 ],,,,,, [ 20 ], [ 12 ],

6 5 : 33,, 810,,,,,, [ 27 ] 312,, [ 28 ],,,,,,, [ 29 ] KITZAN SW EE2 NY [ 30 ] PAS ( P rotopterus annectens) SIBB ING UR IBE [ 31 ] H IDAB 2PAS,, [ 10 ], AB2PAS 4,,, [ 32 ],,,,, [ 29 ],, [ 29 ],,,, 98%,,,, ;, ph I, SIB2 B ING [ 31 ],,,,,, [ 33 ] : [ 1 ],,. [M ]. :, 1962: [ 2 ],,,. [ J ]., 1997, 12 (5) : [ 3 ],,. [ J ]., 2000, 7 (2) : [ 4 ],,. [ J ]., 1996, 31 (1) : 6-9. [ 5 ],,. [ J ]., 1990, 17 (1) : 2-5. [ 6 ],,,. [ J ]., 1987, 6 (3) : [ 7 ],. Rhabdosargus sarba ( Forska1) [ J ]., 2000, 20 ( 3) : [ 8 ],,,. ( ) [ J ]., 1998, 22 (3) : [ 9 ],,,. [ J ]., 2001, 8 (3) : [ 10 ],,. [ J ]., 1997 ( ) :

7 34 4 [ 11 ]. [ J ]., 1987, 7 (4) : [ 12 ],,. [ J ]., 1997, 21 (2) : [ 13 ],,. [ J ]., 1998, 12 (4) : [ 14 ],. [ J ]., 2005, 12 (1) : [ 15 ],,. [ J ]., 1999, 6 (1) : [ 16 ],,,. [ J ]., 2004, 34 (2) : [ 17 ],, [ J ]., 1998, 20 (4) : [ 18 ]. () [M ]. :, 1983: [ 19 ],,,. [ J ]., 1993, 21 (8) : [ 20 ],. [ J ]., 1984, 30 (1) : [ 21 ]. [ J ]. :, 1962, 2 (3) : [ 22 ],. [ J ]., 2003, 34 (4) : [ 23 ],,. [ J ]., 1997, 7 (3), [ 24 ] BUDD INGTON R K, D IAMOND J M. Pyloric ceca of fish: a newabsorp tive organ [ J ]. Am J Physiol, 1987, 252 ( 6 ) : [ 25 ] FERRAR IS R P, TAN J D. Development of the digestive tract of m ilkfish, Chanos chanos ( forsskal) : histology and histochem istry [ J ]. Aquac, 1987, 61 (3 /4) : [ 26 ],,,. [ J ]., 2002, 19 (2) : [ 27 ],. [ J ]., 1963, 3 (3) : [ 28 ],,. [ J ]., 2001, 47 ( ) : [ 29 ],. [ J ]., 1999, 23 (4) : [ 30 ] KITZAN S M, SW EENY P R. A light and electron m icroscope study of the structure of Protopterus annectens epiderm is I. Mucus production [ J ]. Can J Zool, 1968, 46 (5) : [ 31 ] SIBB ING F A, UR IBE R. Regional specializations in the oropha2 ryngeal wall and food p rocessing in the carp ( Cyprinus carpio L. ) [ J ]. Netherland J Zool, 1985, 35 (3) : [ 32 ] SINHA GM. A histochem ical study of the mucous cells in the buc2 co2pharyngeal region of four India freshwater fishes in relation to their origin, development, curarence and p robable functions [ J ]. Acta H istochem Bd, 1975, 53 s (10) : [ 33 ] RE IFE I C W, TRAV ILL A A. Structure and carbohydrate histo2 chemistry of the intestine of ten telesotean species [ J ]. J Morph, 1979, 162 (11) : All Rights Reserved. South China Fisheries Science

8 5 : 35 I(HE ) 11 ( 400) ; 21 ( 400) ; 31 ( 200) ; 41 ( 400) ; 51 ( 400) ; 61 ( 200) ; 71 ( 200) ; 81( 200) ; 9101 ( 200) ; CM1 ; LM1 ; GP1 ; GG1 ; M1 ; SM1 ; V1 ; S1 ; CC1 ; LP1; IG1 ; MC1 ; GC1 ; A1 ; CL1; MM1 ; BD1 ; HP1 ; CV1 ; HA1; AC1 ; IC1 Plate ICross section of digestive system of R1sarba (HE staining) 11 oesophagus ( 400) ; 21 cardiac stomach ( 400) ; 31 pyloric stomach ( 200) ; 41 anterior intestine ( 400) ; 51 m id2intestine ( 400) ; 61 posterior intestine ( 200) ; 71 pyloric caeca ( 200) ; 81 liver ( 200) ; 9101 pancrea near by pyloric caeca ( 200) ; CM1 circular muscle; LM1 longitudinal muscle; GP1 gastric pit; GG1gastric gland; M1 mucosa; SM1 submucosa; V1 vein; S1 serosa; CC1 columner cell; LP1 lam ina p rop ria; IG1 intestinal gland; MC1 muscular coat; GC1 goblet cell; A1 artery; C L1 central lacteal; MM1 muscularis mucosa; BD1 bile ductbiliary duct; HP1 hepatic plate; CV1 central vein; HA1 hepatic artery; AC1 acinar cell; hepatic arteries; IC1 islet cellislet cell All Rights Reserved. South China Fisheries Science

9 36 4 II(AB2PAS ) 11 ( 200) ; 21 ( 400) ; 31( 400) ; 41 ( 400) ; 51 ( 400) ; 61 ( 400) ; 71 ( 400) ; 81 ( 400) I1 I; II1 II; III1 III; IV1 IV Plate IICross section of digestive system of R1sarba (AB2PAS staining) 11 oesophagus ( 200) ; 21 cardiac stomach ( 400) ; 31 undic stomach ( 400) ; 41 pyloric stomach ( 400) ; 51 anterior intestine ( 400) ; 61 m id2intestine ( 400) ; 71 posterior intestine ( 400) ; 81 pyloric caeca ( 400) ; I1 type Imucous cell; II1 type IImucous cell; III1 type IIImucous cell; IV1 type IV mucous cell All Rights Reserved. South China Fisheries Science

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