686 34, 6 µm, HE,,, 20 µm, Ⅲ,,, ; ; ; 100 µm, 10 Excel SPSS 结果 2.1 消化系统的解剖与消化道组织形态指数 4 ( 1),, 1, 1, 1,,, T,,,,,,, ;,,,,,,,, 0.43±0.02, 1.5%± 0.

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1 34 4 Vol. 34, No ACTA HYDROBIOLOGICA SINICA Jul., DOI: /SP.J 波纹唇鱼消化系统的组织学陈国华王永波王珺骆剑林彬张本 (,, ) 摘要 :,,,,, 0.43±0.02;,,,,,,, 3,,,,,,, 1.5%±0.2%;,, 关键词 : ; ; ; 中图分类号 : S917 文献标识码 : A 文章编号 : (2010) (Cheilinus undulates Rüppell),,,, [1 4], [5 7], [8] [9,10], [12 14],,,,,, 1 材料与方法,, 10, (228.9±17.1) mm, (322±62.8) g,, =/ ; = / 100%, Bouin s, 收稿日期 : ; 修订日期 : 基金项目 : (2006AA10A414); - (2007BAD29B03) 通讯作者 : (1956 ),,, ; ; chguh@hainu.edu.cn

2 686 34, 6 µm, HE,,, 20 µm, Ⅲ,,, ; ; ; 100 µm, 10 Excel SPSS 结果 2.1 消化系统的解剖与消化道组织形态指数 4 ( 1),, 1, 1, 1,,, T,,,,,,, ;,,,,,,,, 0.43±0.02, 1.5%± 0.2% ( 1),, ;, 1 ( ; ) Fig. 1 Morphological figure of the digestive system in C. undulates Rüppell(left: digestive gland; right: digestive tract) BC. Buccopharyngeal cavity; OE. Esophagus; AI. Anterior intestine; PI. Pyloric intestine; R. Rectum; L. Liver; G. Gallbladder; A. Anus Spiral valve height(µm) Sub mucosa thick (µm) Circular muscle thick (µm) Longitudinal muscle thick (µm) Serosa thick (µm) Tab. 1 表 1 波纹唇鱼消化道组织形态指数 Features of tissue on digestive tract of C. undulates Esophagus Anterior intestine Pyloric intestine Rectum ± a ±87.06 c ±75.74 c ±71.66 ab ±39.12 a ±30.37 c ±8.50 e ±23.75 g ± a ±1.57 c ±3.64 c ±4.74 e ±8.89 a ±2.08 c ±2.01 c ±4.65 e ±1.38 ac ±1.75 c ±1.17 a ±1.91 d (P>0.05), (P<0.05), (P<0.01); Note: In the table the identical digestive tract character colleague shoulder sign has the same letter expression group the difference not remarkable (P> 0.05), letter neighboring expression group difference remarkable (P< 0.05), the letter great distance expression group the difference is extremely remarkable (P< 0.01); the same below

3 4 : 687 Tab. 2 Absorptive cell (Cell number/100μm) Basal cell (Cell number/100μm) Rodlet cell (Cell number/100μm) Goblet cell (Cell number/100μm) 表 2 波纹唇鱼肠道黏膜上皮各类细胞的相对密度比较 Number of mucosal epithelial cells in digestive tract of C. undulates Anterior intestine Pyloric intestine Rectum 28.85±0.82 a 30.15±0.63 ab 31.23±0.54 b 12.15±0.95 a 11.20±1.00 a 12.00±0.94 a 01.95±0.34 a 01.40±0.26 a 01.62±0.31 a 05.45±0.40 a 05.50±0.37 a 04.23±0.44 b, ;,, ( 2), ;, 2.2 波纹唇鱼消化道的组织学,,,,,,,,,, ( -1) ( -2),,,,, ( -3),, ( -4), ( -5);,,, ( -6), ( -7),,,,, ( -8) ( -9 13, -17),,,, 3,, ( -10, ), ( -10),, Ⅲ, ( -11) ( -19),,, ( , - 20),,,,,,,, ( -21),,

4 688 34, ; ( -22),,, ( ), 2.3 波纹唇鱼消化腺的组织学,,,,,,, ( -25),,,,,,,,,, ( -26),,,,,, ( -27),,,,,,, ( -28),,,,,, HE,,,, ( - 29) ( - 30),,, 3 讨论 3.1 波纹唇鱼肠道的分段,, Mcvay Kann [15] ; [12], ; [16] [13] (Sehizothorax davidi),,,,,,,,,,,,, ;,,, ; ;,,,, 3.2 波纹唇鱼消化道的组织特点与食性的关系, [1,3],,, ;, ;,,,,,,,

5 4 : 689,,,,,,,,,,,,, Himantura signifier,,, [17],,,, (Oplegnathus fasciatus) (Rhabdosargus sarba) (Pagrosomus major),,,,,,,,, [18 20],, [20,21], ;,,,,,,,,,,,,,,, 3.3 波纹唇鱼的消化腺,, (Oreochromis niloticus) [22],,,,,,, 鲌 (Culter alburnus) [23], 参考文献 : [1] Randall J E, Head S M, Sanders A P L, et al. Food habits of the giant humphead wrasse, Cheilinus undulatus (Labridae) [J]. Environmental Biology of Fishes, 1978, 3: [2] Choat J H, Davis C R, Ackerman J L, et al. Age structure and growth in a large teleost, Cheilinus undulatus, with a review of size distribution in labrid fishes [J]. Marine Ecology Progress Series, 2006, 318: [3] Sadovy Y, Kulbicki M, Labrosse P, et al. The humphead wrasse, Cheilinus undulatus: synopsis of a threatened and poorly known giant coral reef fish [J]. Reviews in Fish Biology and Fisheries, 2004, 13: [4] Olivier Chateau, Laurent Wantiez. Site fidelity and activity patterns of a humphead wrasse, Cheilinus undulatus (Labridae), as determined by acoustic telemetry [J]. Environmental Biology of Fishes, 2006, 80(4): [5] Shen S J. Taiwan Ichthyography [M]. Taibei: Taiwan University Press.1993, [.. :. 1993, ] [6] Zhang H Z, Wang A L, Liu Y. Emergency care of patients

6 with Hump Head Wrasse poisoning [J]. Journal of Rare and Uncommon Diseases, 2005, 12(4): [,,.., 2005, 12(4): 28 29] [7] Li G Y, Ma X H. Rescuing and nursing of Su-mei Fish toxicosis patients [J]. Modern Nursin, 2005, 11(10): [,. 32., 2005, 11(10): ] [8] Lou Y D. Histology and Embryology [M]. Beijing: China Agricultural Press. 1998, [.. :. 1998, ] [9] Yu Z N, Sun X Y, Sun S C. A study of the histology and morphology of digestive tract of the Chrysopherys major [J]. Journal of Fisheries of China, 1997, 21(2): [,,.., 1997, 21(2): ] [10] Wang J X, Shi G, Li P, et al, Morphology and histology of digestive tract in Oplegnathus fasciatus [J]. Journal of Fisheries of China, 2006, 30(5): [,,,.., 2006, 30(5): ] [11] Gou X W, Ou Y J, Liao R. Histological and histochemical study of digestive system of Rhabdosargus sarba [J]. South China Fisheries Science, 2008, 4(5): [,,.., 2008, 4(5): 28 36] [12] Lin H R. Digestive tract of five different feeding habits carp branch fish [J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, : [.. ( ), 1962, 3: 65 78] [13] He M. Histological and histochemical study of digestive tract of Schizothorax davidi Sauvage [D]. Sichuan Agricultural University [ ] [14] Zhang J H, Wang S Y. Studies on the anatomy of intestine of varicorhinus macrolepi [J]. Journal of Shandong Agricultural University (Natural Science), 2003, 34(4): [,.. ( ), 2003, 34(4): ] [15] Mcvay J A, Kann H W. The digestive tract of Carassius auratus [J]. Biol Bull, 1940, 78: [16] Ni D S, Hong X F. Histology of digestive tract in Cienopharyngodon idellus [J]. Acta Hydrobiologica Sinica, 1963, 3: 1 25 [,.., 1963, 3: 1 25] [17] Kannika C, Ricardo M, Jintana P, et al. Histology of the digestive tract of the freshwater stingray Himantura signifer Compagno and Roberts, 1982 (Elasmobranchii, Dasyatidae) [J]. Anat Embryol, 2006, 211: [18] Dezfuli B S, Giari L, Konecny. Immunohisto chemistry, ultrastructure and pathology of gills of Abramis brama from LakeMondsee, Austria, infected with Ergasilus sieboldi (Copepoda) [J]. Diseases of Aquatic Organisms, 2003, 53: [19] Manera M, Dezfuli B S. Rodlet cells in teleosts: a new insight into their nature and functions [J]. Journal of Fish Biology, 2004, 65: [20] Reite O B. The rodlet cells of teleostean fish: their potential role and host defence in relation to the role of mast cells/eosinophilic granule cells [J]. Fish and Shellfish Immunology, 2005, 19: [21] Ingvar L. Leknes. Eosinophilic granule cells and endocytic cells in intestinal wall of pearl gouramy (Anabantidae: Teleostei) [J]. Fish and Shellfish Immunology, 2007, 23: [22] Antonio M. Figueiredo-Fernandes, Antonio A. Spatial relationships of the intrahepatic vascular biliary tracts and associated pancreatic acini of Nile tilapia, Oreochromis niloticus (Teleostei, Cichlidae): A serial section study by light microscopy [J]. Ann Anat, 2007, 189: [23] Xiong H L. Morphological studies on livers and pancreases of Culter alburnus, Mystus macropterus and Siniperca scherzeri [D]. Southwest University [. 鲌, 鳠 ]

7 4 : 691 HISTOLOGY OF THE DIGESTIVE SYSTEM IN CHEILINUS UNDULATES RÜPPELL CHEN Guo-Hua, WANG Yong-Bo, WANG Jun, LUO Jian, LIN Bin and ZHANG Ben (Key Laboratory of Tropic Biological Resources of Ministry of Education, College of Ocean, Hainan University, Haikou ) Abstract: This paper reported the histological studies of the digestive system of the humphead or Napoleon wrasse (Cheilinus undulates Rüppell) by light microscopy of tissue sections, and described the morphological features of the digestive system of this species. The humphead wrasse, a member of the family Labridac, is a species of stomachless and carnivorous fish. The digestive tract of these fish consists of oral-pharyngeal cavity, esophagus, small intestine, rectum and anus. Their jaw teeth and pharyngeal tooth in the oral-pharyngeal cavity are tough and strong. The intestine is short in length and wide in diameter, and curve-shaped. The average ratio between the length of the intestine and the body length is Histological studies showed that the esophagus mucosa layer of the humphead wrasse was covered with wrinkles and folds. The submucosa, longitudinal muscle and circular muscle layers of the esophagus were well developed. The submucosa, circular muscle, longitudinal muscle and serosa layers of the small intestine were relatively thinner than those of the rectum. The submucosa and serosa layers of the anterior intestine were relatively thicker than those of the posterior intestine. The density of Paneth cells in the mucosa layer of the small intestine was lower than that of the rectum while the density of goblet cells in the mucosa layer of the small intestine was higher than that of the rectum. The mucosa epithelia of the oral cavity and tongue of this species were stratified squamous epithelia with abundant rounded mucous cells. The taste buds on the tongue and oral cavity were observed. The mucosa layer of the esophagus was well developed. In the proximal esophageal mucosa, a concave-shaped structure was observed in which abundant giant mucous cells distributed. This structure was similar to the exocrine gland, and had an opening at the esophagus. The epithelial cells of the small intestine and rectum were monolayer columnar covered with microvillus; and three kinds of goblet cells were observed. In the submucosa layer, there were many eosinophilic granule cells of which the cytoplasm was stained by eosin. Lipochondria were observed in the monolayer columnar epithelial cells of the anterior intestine. It was also observed that some of the cells were stained by eosin in the epithelium of the rectum. The valves were found between the esophagus and small intestine and between the small intestine and rectum. The muscle layer in the valve consisted of striated muscle. The mucosa layer of the rectum contained a large amount of oval-shaped mucous cells. The digestive gland consisted of hepatopancreas and cholecyst. The liver of the fish had three lobes and was maple leaf-shaped; the pancreas dispersedly distributed in the liver along its blood vessels; gallbladder had an oval shape with a relatively large volume. We also discussed the segments of the intestine, histological features of the alimentary tract of this species, and their relation with the eating habits of the humphead wrasse. Key words: Cheilinus undulates Rüppell; Histology; Digestive tract; Digestive gland

8 692 水生生物学报 34 卷图版Ⅰ PlateⅠ 1. 口咽腔 Oropharyngeal cavity; 2. 舌纵切 Longitudinal section of tongue; 3. 舌示味蕾 Taste bud of tongue; 4. 食道横切 Cross-section of esophagus; 5. 食道上皮黏膜 Single columnar epithelium of esophagus; 6. 食道类似外分泌腺结构 Analogues of exocrine glands of esophagus; 7. 食道上皮组织中的脂肪层 Fat of esophagus; 8. 食道肌肉层 Muscular of esophagus; 9. 小肠前部横切 Cross-section of anterior intestine; 10. 小肠前部上皮组织 Epithelium of anterior intestine; 11. 小肠前部组织中的脂肪层(苏丹Ⅲ染色)Fat of anterior intestine(sudan Ⅲ); 12. 小肠前部黏膜下层 Sub mucosa of anterior intestine; 13. 小肠后部横切 Cross-section of pyloric intestine; 14. 小肠后部黏膜下层 Sub mucosa of pyloric intestine; 15. 小肠后部上皮组织 Eosinophilic granule cells of pyloric intestine AEG. 外分泌腺类似物 Analogues of exocrine glands; AV. 动脉血管 Artery vascular; BAB. 舌基骨 Basihyal bone; BB.纹状缘 Brush border; CM. 环肌 Circular muscle; CP. 轮廓乳头 Circumvallate papillae; E. 上皮 Epithelium; EGC. 嗜酸性颗粒细胞 Eosinophilic granule cells; FL. 脂肪层 Fat layer; GC. 杯状细胞 Goblet cell; LM. 纵肌 Longitudinal muscle; MB. 肌肉束 Muscular bundle; MC. 黏液细胞 Mucous cell; ML. 肌层 Muscular layer; NGC. 对 HE 负染色的杯状细胞 Haematoxylin and eosin negative goblet cells; PGC. HE 正染色的杯状细胞 Haematoxylin and eosin positive goblet cells; RC. 梨状细胞 Rodlet cell; SCE. 单层柱状上皮 Single columnar epithelium; S. 浆膜 Serosa; SM. 黏膜下层 Sub mucosa; TB. 味蕾 Taste bud; V. 血管 Vascular

9 4期陈国华等: 波纹唇鱼消化系统的组织学 693 图版Ⅱ Plate Ⅱ 16. 小肠后部的上皮组织 Epithelium of pyloric intestine; 17. 直肠横切 Cross-section of rectum; 18. 直肠上皮组织 Epithelium of rectum; 19. 直肠上皮组织中的嗜酸性细胞 Eosinophilic granule cells of rectum; 20. 直肠黏膜下层组织 Sub mucosa of rectum; 21. 食道与小肠的纵切 Longitudinal section of esophagus anterior intestine junction; 22. 小肠与直肠的纵切 Longitudinal section of pyloric intestine rectum junction; 23. 直肠与肛门的纵切 Longitudinal section of rectum anus; 24. 肛门的上皮组织 Epithelium of anus; 25. 肝小叶 Hepatic lobules; 26. 肝细胞 Liver cells; 27. 胰脏在肝脏中的分布 The distribution of pancreas in liver; 28. 胰腺泡 Pancreatic acini; 29. 胰脏与胰岛 Pancreas and islet; 30. 胆囊组织 Gallbladder CC. 泡心细胞 Centroacinar cell; CCV. 中央乳糜管 Central chyle vessel; EC. 内皮细胞 Endothelial cell; GB. 胆囊 Gall bladder; HC. 肝细胞索 Hepatic cord; HD. 肝管 Hepatic duct; He. 肝细胞 Hepatocyte; HS. 肝血窦 Hepatic sinusoid; L. 肝脏 Liver; PA. 胰腺泡 Pancreatic acini; Va. 瓣膜 Valve

Microsoft Word - 13-区又君.doc

Microsoft Word - 13-区又君.doc 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 海洋生物学 doi:10.3969/j.issn.1009-5470.2012.06.013 2012 年第 31 卷第 6 期 : 83 89 http://www.jto.ac.cn 波纹唇鱼消化道的形态学和组织学区又君 1, 廖光勇 1,2, 李加儿