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1 5 7 Vol. 5 No Journal of Food Safety and Quality Jul., 2014 HPLC 李强, 任虹 *, 王友升, 李婷 (,,,, ) 摘要 : 目的方法 Folin-Ciocalteu, HPLC ; (ORAC) 结果 1.26 mg/g,,, ORAC μmol TE/g 结论, 关键词 : ; ; Study on phenolics composition of corn silk and its antioxidant activity in vitro LI Qiang, REN Hong *, WANG You-Sheng, LI Ting (Beijing Key Laboratory of Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory forfood Quality and Safety, Food Science College of Beijing Technology & Business University, Beijing , China) ABSTRACT: Objective To analyze the phenolics composition of corn silk and its antioxidant activity in vitro. Methods Folin-Ciocalteu method was used to evaluate the amounts of total phenolics of corn silk. Phenolics composition of corn silk was analyzed by HPLC and the antioxidant activity was evaluated by ORAC method. Results The amount of phenolics in corn silk was 1.26 mg GAE/g. HPLC analysis revealed that 6 phenolic acids (including catechinic acid, chlorogenic, caffeic acid, ferulic acid, phlorizin, and quercetin) were detected. Antioxidant activity assay showed that phenolics of corn silk had an effective antioxidant activity and the average ORAC value was μmol TE/g. Conclusion Corn silk is rich in polyphenols with antioxidant activity. KEY WORDS: corn silk; phenolics; antioxidant activity 1 引言 (corn silk) (Zea mays L.) [1],,,, [2-6] ;, [7], 基金项目 : (863 ) (2011AA060701) Fund: Supported by High Technology Researchand Development Program of China (863 Program) (2011AA060701) * 通讯作者 :,,, renhong@th.btbu.edu.cn *Corresponding author: REN Hong, Associate Professor, Beijing Technology & Business University, NO.11, Fucheng Road, Haidian District, Beijing , China. renhong@th.btbu.edu.cn

2 2004 5, [8], Folin-Ciocalteau, (HPLC), ORAC, 2 材料与方法 2.1 材料与试剂 ; E (FL) 2,2- (AAPH) ( ) ; Folin-Ciocalteu ;, 2.2 仪器与设备 ( ); ( ); SPECTRA MAX190 (Molecular Devices); (Waters 2695 ); GEMINI EM (Molecular Devices) 2.3 方法玉米须多酚物质的提取 0.5 g, 10 ml 80% 1 ml 1 mol/l HCl 28 2 h,,, 10 ml 80%,, 40,,, 玉米须多酚总含量的测定 g/l, ml 10 ml, 10 ml, μg/ml 20 μl 96, 140 μl, 10 μl Folin-Ciocalteu, 30 μl,, 25 2 h, 760 nm, mg/ml, 20 μl 96, , 140 μl, 10 μl Folin-Ciocalteu, 30 μl,, 25 2 h, 765 nm, HPLC [9] : ZORBAX SB-C18 (250 mm 4.6 mm, 5 µm), A (ph2.5), B, : 90%~ 50% B(0~20 min), 50%~60% B(20~30 min), 60% 100% B(30~40 min), 1.0 ml/min, 10 µl, 30, 280 nm ORAC 法测定玉米须多酚提取物抗氧化活 [10-12] 性 g 1.23 g, 500mL, 75 mmol/ L ph FL FL 37.6 mg,, 10 mmol/l FL, FL a FL a 50 μl 50 ml,, mmol/l, FL b; FL a b 4 FL b 500 μl 50 ml,, mmol/l AAPH AAPH mg, 10 ml, 100 mmol/l AAPH Trolox Trolox 2.50 mg,

3 7, : HPLC ml, 1 mmol/l Trolox 1 mmol/l Trolox μl 5 ml,, mol/l Trolox Trolox 96 Trolox 20 μl 20 μl FL 20 μl, 37 5 min, AAPH 140 μl, nm, 538 nm, 2 min (F n ), ORAC, FL (-AAPH) (+AAPH) F n AAPH, f n, (AUC), : AUC=2 (f 0 +f 1 + +f n-1 +f n ) f 0 f n (1) (Net AUC), : Net AUC = AUC Trolox AUC +AAPH (2) Trolox, Net AUC, ORAC , ORAC Trolox, Net AUC Trolox, Trolox, ORAC 3 结果与分析 3.1 玉米须中多酚总含量 (x) (y) y = x ,,, 1.26 mg/g,,,,, mg/g,, mg/g, 1 Fig. 1 Table 1 1 The linear relationship between A and gallic acid concentration 表 1 玉米须多酚总含量 The amounts of total phenolics of corn silk mg/g 0.671± ± ± 玉米须多酚成分分析 HPLC, 10 ( ) 2.3.2,, min,,, 2 [13],, 3.3 抗氧化活性分析 3, Trolox,, Trolox Trolox y=0.35x , R 2 =0.9902, 20~80 µmol/l Net AUC Trolox, ORAC μmol TE/g [14] / (FRAP) 80% FRAP µmol/l [15]

4 HPLC (a 10, b, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ) Fig. 2 The HPLC chromatogram of phenolics extract from corn silk(a showing the separation of standard phenolic compounds, b showing the separation of phenolics compounds in corn silk. 1 gallic acid, 2 protocatechuic acid, 3 catechinic acid, 4 chlorogenic acid, 5 epicatechin, 6 caffeic acid, 7 ferulic acid, 8 phlorizin, 9 quercetin, 10 kaempferol) DPPH Fe 3+ : ; DPPH IC mg/ml, BHT IC 50 (0.17 mg/ml);, Fe 3+ BHT 4 结论 Folin-Ciocalteu,, ORAC, 1.26 mg/g,, ORAC μmol TE/g,,, AAPH 3 Trolox (A) Trolox (B) Fig. 3 Effect of Trolox on FL fluorescence decay curve in different concentrations (A), the linear relationship between Net AUC and Trolox concentration (B),,, 参考文献 [1],,,. [J]., 2011, 39(3): Zhou HL, Zhang Y, Zhang Y, et al. The development and utilization prospects of corn stigma [J]. Jiangsu Agric Sci, 2011, 39(3): [2] Velazquez DVO, Xavier HS, Batistac JEM, et al. Zea mays L. extracts modify glomerular function and potassium urinary excretion in conscious rats [J]. Phytomed., 2005, 12(5): [3] Guo JY, Liu TJ, Han LN, et al. The effects of corn silk on glycaemic metabolism [J]. Nutr Met, 2009, 6: [4] Liu J, Wang CN, Wang ZZ, et al. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea

7, : HPLC 2007 mays L.) and related flavone glycosides [J]. Food Chem, 2011, 126(1): 261 269. [5] Zhao WZ, Yin YG, Yu ZP, et al.

Anti-inflammation effects of corn silk in a rat model of carrageenin-induced pleurisy [J]. Inflammation, 2012, 35 (3): 822 827. [7] Khairunnisa H, Puziah H, Shuhaimi M.

5 7, : HPLC 2007 mays L.) and related flavone glycosides [J]. Food Chem, 2011, 126(1): [5] Zhao WZ, Yin YG, Yu ZP, et al. Comparison of anti-diabetic effects of polysaccharides from corn silk on normal and hyperglycemia rats [J]. Int J Biol Macromol, 2012, 50 (4): [6] Wang GQ, Xu T, Bu XM, et al. Anti-inflammation effects of corn silk in a rat model of carrageenin-induced pleurisy [J]. Inflammation, 2012, 35 (3): [7] Khairunnisa H, Puziah H, Shuhaimi M. Corn silk (stigma maydis) in healthcare: a phytochemical and pharmacological review [J]. Molecules, 2012, 17(8): [8],,. [J]., 2007, (4): Kuang X, Kuang R, Zhu HT. Chemical components of corn beard and its pharmacological effects and health care functions [J]. Food Nutr China, 2007, (4): [9] Mar Win AAH, Badlishah SB, Farooq A. Effects of roasting on phenolics composition and antioxidant activity of peanut (Arachis hypogaea L.) kernel flour [J]. Eur Food Res Technol, 2011, 233(4), 4: [10],,,. ORAC [J]., 2011, 37(10): Cao YL, Zhao MM, Zheng SJ, et al. Optimization on preparation flavonoids and phenolic acids and 9 extracts of Chinese materia medica [J]. China J Chin Mater Med, 2010, 35(17): [13],,,. [J]., 2010, 31(10): Chen ZY, Xu YJ, Yin Y, et al. Analysis of polyphenol composition of different parts of an ear of sweet corn [J]. Food Sci, 2010, 31(10): [14],,. [J]., 2008, (4): Fang M, Gong ZY, Wang YF. Study on antioxidative activities of corn silk of ethanol extracts in vitro [J]. Food Nutr China, 2008, 4: [15],,,. [J/OL]., TS html. Liu L, Liao L, Hu JZ, et al. Research on antioxidant activity of total flavonoids of corn silk [J/OL]. Sci Technol Food Ind, net/kcms/detail/ TS html. ( 责任编辑 : 杨翠娜 ) 作者简介 of soybean antioxidative peptide with orac assay of evaluation [J]. Food Ferm Ind, 2011, 37(10): [11],,. (ORAC) [J]., 2006, 22(8): 李强, 在读硕士研究生, 主要研究方向为天然产物化学 lyqiangli@163.com Xu JK, Yao XS, Li YB. Oxygen radical absorbance capacity assay and its application [J]. Chin Pharmacol Bull, 2006, 22(8): [12],, AAPH [J]., 2010, 35(17): 任虹, 博士, 副教授, 主要研究方向为天然产物化学 renhong@th.btbu.edu.cn Dai HQ, Chen CY, Yang B. AAPH scavenging activities of 22

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填表说明 1. 本表所填数据截至时间为 2011 年 9 月底 2. 本表请用 A4 纸双面打印, 加盖学校公章后上报 3. 表内所填数据请学校认真核实, 确保准确无误 4. 建设完成情况对照填表说明 1. 本表所填数据截至时间为 2011 年 9 月底 2. 本表请用 A4 纸双面打印, 加盖学校公章后上报 3. 表内所填数据请学校认真核实, 确保准确无误 4. 建设完成情况对照表等相关表格栏高不够请自行增加 5. 表格中填写的相关量