2017 7,, 1.3.4, (PEG)/ (1)PEG 20 ml,, 5.0 g, 4.0 g PEG2000 PEG4000, PEG6000 PEG8000 PEG10000,, 40min, (2)PEG : 20 ml,, 5.0g,PEG ,3.5,4.0,

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33 7 2017 7 Vol.33,No.7 Jul.2017 DOI:10.13652/j.issn.1003-5788.2017.07.

1 Vol.33,No.7 Jul.2017 DOI: /j.issn Extractionofpolysaccharidefromgerminatedbrownricebyaqueous two-phasesystemandresearchofitsantioxidantactivity 1, LIU Xiao-fei 1,2 GONG Li-li 2 WANG Xin 1 MENG Qing-hong 1 MA Yong-qiang 2 ZHANG Yu LU Shu-wen 1 (1., , ) (1.Heilongjiang Academyof AgriculturalSciences PostdoctoralProgramme,Harbin,Heilongjiang150086,China 2.Key Laboratory for Food Scienceand Engineering,Harbin Universityof Commerce,Harbin,Heilongjiang150076,China) mined.results were showed as folows:the mass fraction of PEG6000was15.7%,themassfractionofammonium sulfatewas 14.8% andextractiontimewas40 min.theyieldofpolysaccharide substancefrom germinatedbrownricewas81.07% intheoptimum conditions.morever,theanti-oxidationofpolysaccharidesubstance fromgerminatedbrownricewerestudiedbymethodsofdpph,py-, rogalolautoxidationandphenanthroline.theresultsshowedthat, thepolysaccharideofbrownricehadstrongerantioxidantefectto, DPPH,ultraoxygenanionandhydroxylradicals. : Keywords:Germinatedbrownricepolysaccharideultrasonictwo- phaseextractionantioxidantactivity PEG6000 PEG % 14.8% 40min, 81.07%, DPPH, DPPH [1],, γ- [2-3], Abstract:Thepolysaccharidewasextractedbytheultrasonic-assisted, aqueoustwo-phasetechnologyfrom germinated brown rice.the [4] phaseseparationabilityofdiferentinpeg/(nh4) 2SO4system was studiedaccordingtothepolysaccharideyield.theyieldofpolysac- charidesubstanceofgerminatedbrownrice wasdeterminedbyor- thogonalexperiment.theoptimum processconditions weredeter- (LBH-Z15200), ( ),,,, (1968 ),, [14-15] γ- [email protected] , [5-6], [7] [8], PEG 3, 55.1% XingJianmin [9], [10],, /K2CO3 [11-13] [16-17] [18-19], 149

2 2017 7,, 1.3.4, (PEG)/ (1)PEG 20 ml,, 5.0 g, 4.0 g PEG2000 PEG4000, PEG6000 PEG8000 PEG10000,, 40min, (2)PEG : 20 ml,, 5.0g,PEG ,3.5,4.0,4.5, g,, 40 min :JY92-ⅡD,,, (3) : 20 ml, :SP-752PC, PEG g, 3.0,4.0,5.0, 6.0,7.0g,, 40 min :TGL-16G,,, HHS-12, 5.0g,PEG g,, :BS224S,, , (1)DPPH [23], (4), 2h, DPPH D, ( 30 A0 - ( Ai -Aj ) D = 100%, (4) 98% 24h) [20] A min, 55, (80) [19] g,, 1 10 (g/ml), 500 W 25min [22], 50,,3000r/min 20min,, 1000mL, (5) O2 - A 20mL, PEG, ΔA0 -ΔA,, 40 min A = 100%, (5) ΔA0,620nm, [21-22] K = Ca, (1) Cb R = Va, (2) Vb Y = RK (6) OH E 1+RK, ( 3) A -A E = 100%, (6) A -A K PEG/ E OH,% 150 R Y,% Ca,mg/mL Cb,mg/mL Va,mL Vb,mL (4) 20mL, 20,30,40,50,60min, D DPPH,% A0 Ai A j (2) (O 2 - : [24], A O 2 -,% ΔA0 ΔA (3) ( OH) : [25], A A A

3 Figure1 Thestandardcurveofglucose :y=0.012x ,r 2 =0.9896, 2.2,, PEG PEG 15.7% (65.03%), PEG,, 2~4 2~4,,PEG, R, K PEG, PEG, [8], K,PEG6000,,PEG6000, 75.27% PEG 5~7 5~7, PEG, R, K, [28] 2 K PEG Figure2 ThecorrelationofpartitioncoeficientK and PEG molecularweight 5 K PEG Figure5 ThecorrelationofpartitioncoeficientK and PEGconcentration 3 PEG Figure3 ThecorrelationofvolumeratioandPEG molecularweight 6 PEG Figure6 ThecorrelationofvolumeratioandPEG concentration 4 PEG Figure4 Thecorrelationofpolysaccharidesyieldand PEG molecularweight 7 PEG Figure7 Thecorrelationofpolysaccharidesyieldand PEGconcentration 151

2017 7 2.2.3 8~10 8~10,,2 14.8%,, 20.6%, 2, 11 K 14.8% (73.25%) Figure11 ThecorrelationofpartitioncoeficientK and [27], PEG, 14.8% extractiontime 2.2.4 11~13 11~13,,, 40min, 12 Figure12 Thecorrelationofvolumeratioand extractiontime 8 K Figure8 ThecorrelationofpartitioncoeficientK and concentrationofammoniumsulfate 40min 9 2.

4 ~10 8~10,,2 14.8%,, 20.6%, 2, 11 K 14.8% (73.25%) Figure11 ThecorrelationofpartitioncoeficientK and [27], PEG, 14.8% extractiontime ~13 11~13,,, 40min, 12 Figure12 Thecorrelationofvolumeratioand extractiontime 8 K Figure8 ThecorrelationofpartitioncoeficientK and concentrationofammoniumsulfate 40min Figure9 Thecorrelationofvolumeratioandconcentration, ofammoniumsulfate PEG PEG, Figure10 Thecorrelationofpolysaccharidesyieldand concentrationofammoniumsulfate Figure13 Thecorrelationofpolysaccharidesyieldand extractiontime,,,, 2, : PEG > >PEG > A2 B3 C2 D2, PEG6000 PEG % 14.8% 40min,, 3,, 81.07% DPPH DPPH 14

5 Table1 Orthogonalarraydesign APEG BPEG C D /% /% /min Figure15 Scavengingefectofpolysaccharidefrom germi- Table2 Experimentaldesignandresultsofpolysaccharide natedbrownricebyo 2 - method fromgerminatedbrownrice A B C D /% k k k ( OH) 16 16,, 0.5 mg/ml,, 53%, [29] R Figure16 Scavengingefectofpolysaccharidefrom germi- natedbrownriceby OH method 14 DPPH Figure14 Scavengingefectofpolysaccharidefrom germi- natedbrownricebydpph method 3, PEG6000 PEG % 14.8% 40 min, 81.07%,,DPPH 14,DPPH 81.85%,,, 0.6 mg/ml 68.77%,53.00%,DPPH 81.85% DPPH (O 2 - [1],,. ) [J].,2014(5): [2],,,. 15, <0.3mg/mL, [J].,2011,27(6): %, [3]CHO DongHwa,LIM SeungTaik.Germinatedbrownriceand, itsbio-functionalcompounds[j].food Chemistry,2016, mg/mL, 68.77%, (8): [4],,. [J]. 153

6 2017 7,2009(10):1-4. onicliquids[j].chemsocrev,2012,41(14): [6],,,.,2016,29(11): [J].,2012,28(5): [7],,. γ- [J].,2009,30(13): [J].,2013,34(24): [8],,,. [21]. [J].,2013,32(7): technology,2009,158(1): [10],. [24],. [J].,2016,42(5): [11],,,. [J]. [25],,,.,2009,30(16): [12],,,. [26],,,. [J].,2013,29(11): [13],. γ- [J]. [27]GEORGE Z, GIOKAS D, VLESSIDIS A.The efects of,2013,20(5): solventpreoxidationoninhibitedchemiluminescenceofpyro- [14],,. γ- galoloxidationinflowinjectionanalysisandliquidchromatog- [J].,2015,63(6): raphy[j].analyticachimicaacta,2006,565(1): [15]. γ- [28],,. [D].,2006. [16]. [J].[29],,.,2016,37(22): [17],,, [J].,2011,32(11): [18],,. [J].,2017,33(1): [19],,. [J]. [20],,,. [J].,2013(25): [22],,. [J].,2016,37(23): [23],,,. [J].,2014,39(3): [J].,2011,32(7):4-6. [J].,2016,32(7): [J].,2010,26(2): [5]FREIRE MaraG,CLAUDIO AnaFilipaM,ARAUJOJM,et al.aqueousbiphasicsystems:aboostbroughtaboutbyusingi- [9]XINGJian-min,LIFen-fang.Separationandpurificationofaloe polysaccharidesbyacombinationofmembrabeultrafiltrationand aqueoustwo-phaseextraction[j].appliedbiochemistryandbio- [J].,2017,38(3): [J].,2017,38(3): ( 69) (11): , [7],,. [J].,2011,30(4): [8],,,.,, [J].,,2007,27(1): [9]. [D]. :,2009:8-12. [10],,,. [1],,. [J]. :,2013,49(7): [J] ,2016,26(1): [11]SARZANINIC,SACCHERO G,ACETO M,etal.Simultane- [2],,,. ousdetermination of methyl-,ethyl-,phenyl-and inorganic [J].,2016(3): mercurybycoldvapouratomicabsorptionspectrometry with [3],,,. on-linechromatographicseparation[j].journalofchromatogra- [J]. phya,1992,626(1): ,2016,52(11): [12]FALTER R,SCHOLER H F.Interfacinghigh-performanceliq- [4],,.HPLC-ICP-MS uidchromatographyandcold-vapouratomicabsorptionspec- [J].,2009,18(1): trometrywithon-line UVirradiationforthedeterminationof [5],,,. organicmercurycompounds[j].journalofchromatographya, [J].,2014,39(5): ,675(1/2): [6],,,. [13],. [J].,2016, [J].,2002,18(4):

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( ) [10] [2] ( ) ( ) [11] [15] 1995 ( ) [12] 1996 ( ) [13] 2005 ( ) ( ) [14] [2] ( ) 4 [13] ( ) 33 7 2017 7 Vol.33No.7 Jul.2017 DOI:10.13652/j.issn.1003-5788.2017.07.043 ResearchprogressofChinesemultiple-flavorliquor CAO Jing YU You-gui CAO Zhi-hua ZENG Hao ( 422000) (Colegeof Food Scienceand ChemicalEngineeringShaoyang