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1 2015 9, 22(5): Journal of Fishery Sciences of China 研究论文 DOI: /SP.J LC-PUFA 1, 2 1, 谢帝芝, 徐树德 3 1, 2, 陈芳, 王树启 1, 游翠红 1 1, 李远友 1., ; 2., ; 3., 摘要 : (Siganus canaliculatus) (long-chain polyunsaturated fatty acids, LC-PUFA), (FO) (, VO), 3 ( ) 8, Na + /K + -ATPase (NKA),, VO FO (P>0.05); FO n-3 LC-PUFA VO (P<0.05), VO n-6 LC-PUFA FO (P<0.05); VO NKA mrna FO (P<0.05), VO FO, (P<0.05), LC-PUFA NKA mrna (P<0.05), (P>0.05), 10,, n-3 LC-PUFA ;, n-6 LC-PUFA NKA : ; ; ; Na + /K + -ATPase; ; LC-PUFA 中图分类号 : S963 文献标志码 : A 文章编号 : (2015) Na + /K + -ATPase (NKA),, [1], NKA,, NKA mrna [2 7],, (arachidonic acid, ARA) [8], ARA (Sparus aurata) 鮨 (Centropristis striata)nka [9 10] NKA, LC-PUFA,, [11] Nano [12] LC-PUFA Caco-2,,, LC-PUFA,, (Salmo salar) [13] (Siganus canaliculatus) [14] (Pagrus major) [15] 收稿日期 : ; 修订日期 : 基金项目 : ( ); ( ); ( , ). 作者简介 : (1986 ),,,,. xiedizhi@qq.com 通信作者 :,,. yyli@stu.edu.cn

2 5 : LC-PUFA 951 LC-PUFA, LC-PUFA, LC-PUFA,, LC-PUFA [14] LC-PUFA, NKA,, ,, NKA, LC-PUFA,, g ( 32) 1, 3 :, , 3 2, 20,, (FO) (, VO), 350 g/kg 80 g/kg,, ( ),, , 24 h, 0.01% (2-phenoxyethanol),, ( 0.9 m, 1 m), 20, 6, 3, 1, 1/2, ; 2 (8:30, 16:30), ;, ; (22±3),, 3 3, ; 4 ( 12 ) 6, 80 C, [14, 16] 1.3, 0.01% (butylated hydroxytoluene, BHT) : 30 mg 500 μl ; Waterman (Waterman, ) ;, 25 ml/min 20 ml ;, 30 ml, (ca. 48%, Acros Organics, USA) GC-17A ( : VF-23 ms, 30 m 0.25 mm 0.25 μm) Sigma, Li [14, 16] 1.4 NKA NKA

3 ingredient Tab. 1 表 1 实验饲料配方及营养成分 Ingredients and composition of experimental diets FO dietary group % dry matter basis VO soy protein concentrate dextrin α- α-starch cellulose mineral mix vitamin mix choline chloride dicalcium phosphate C vitamin C L- L-lysine DL- DL-methionine betaine Cr 2 O fish oil canola oil perilla oil proximate composition dry matter crude protein crude lipid ash content : 1. : 100 g, 30 g, 8 g, 1 g, 30 g, 2 g, 1 g, 500 mg, 40 mg. 2. : A IU, D IU, E 60 g, K 3 6 g, B g, B 2 16 g, B 6 12 g, B mg, 88 g, 36 g, 2 g, 100 mg, 100 g, 200 g.. Note: 1. One kilogram of mineral mix containing the following: K 100 g, Mg 30 g, Fe 8 g, Mo 1 g, Zn 30 g, Mn 2 g, Co 1 g, I 500 mg, Se 40 mg. 2. One kilogram of vitamin mix containing the following: A IU, D IU, E 60 g, K 3 6 g, B g, B 2 16 g, B 6 12 g, B mg, nicotinic acid 88 g, calcium pantothenate 36 g, folic acid 2 g, biotin 100 mg, inositol 100 g, C-monophopholipid 200 g. Supplied by Chengyi Co., Ltd., China. 1.5 RNA PCR Trizol (Invitrogen, ) RNA RNA gdna cdna β-actin, PCR (Real- time PCR) NKA (C T ) [17] 3 NKA (GeneBank : EU107280) β-actin (EU ), 2 Real-time PCR : ddh 2 O 6 μl, SYBR Green Supermix 10 μl, (10 μmol/l) 1 μl, cdna(10 ng/μl) 2 μl : 94 5min; s, s, s, 45 ; 95 5 s, 65 1 min, s ± ( x ±SE), SPSS 17.0 (ANOVA), 0.05

4 5 : LC-PUFA ,, (P>0.05),, VO FO, (P<0.05), (P>0.05),, (P>0.05), 10 (P<0.05, 3) 表 2 关键酶基因实时定量 PCR 引物 Tab. 2 Primers for real-time PCR determination of the key enzyme genes expression transcript primer (5 3 ) sequence (5 3 ) GenBank accession no. NKA NKA S TGGTTCCCGCCATCTCTCT NKA A TCAGCAGGTCCATCGGGTAG EU β-actin BS CTTCCTTCCTCGGTATGGAGTC BA AGGTGGAGCAATGATCTTGATC EU Tab. 3 表 3 不同处理组黄斑蓝子鱼生长性能和常规生化指标 Growth performance and proximate composition of Siganus canaliculatus at different treatments treatment n=3; x ±SE; % %wet weight basis growth performance VO FO VO FO VO FO /g initial body weight, IBW 17.59± ± ± ± ± ±0.16 /g final body weight, FBW 34.37±0.58 b 37.98±2.24 b 43.94±0.85 a 46.99±0.90 a 42.79±2.38 a 46.28±0.92 a /% weight gain rate 95.38±8.51 c ±13.21 bc ±7.09 ab ±2.73 a ±4.40 ab ±2.89 a / (% d 1 ) special growth rate 1.16±0.04 c 1.33±0.11b c 1.54±0.13 ab 1.65±0.02 a 1.52±0.05 ab 1.69±0.02 a feed conversion ratio 1.74± ± ± ± ± ±0.07 proximate composition moisture 71.13±0.43 a 71.34±0.59 a 69.14±0.81 b 67.51±0.41 b 65.64±0.41 b 67.18±0.85 b crude protein 17.50±0.44 b 17.92±0.45 ab 17.95±0.42 ab 19.44±0.18 a 19.11±0.76 ab 20.16±0.59 a crude lipid 9.38±0.33 b 9.16±0.33 b 11.08±0.78 a 10.89±0.12 a 12.10±0.69 a 11.52±0.22 a ash 3.18±0.16 a 3.01±0.16 ab 2.46±0.28 b 2.30±0.39 b 2.85±0.11 b 2.51±0.09 b : (P<0.05). (%)=100 ( )/ ; (% d 1 )=100 [ln( ) ln( )]/ ; = (g, )/ (g, ). Note: Values within the same row with different letters are significantly different (P<0.05). Weight gain rate (%) =100 (FBW IBW)/IBW. Special growth rate (% d 1 )=100 [ln(fbw) ln(ibw)]/days. Feed conversion ratio= dry feed consumed (g)/wet weight gain (g) , FO VO, (saturated fatty acids, SFA) (monounsaturated fatty acids, MUFA) C 18 PUFA LC-PUFA,, 10 n-3 LC-PUFA n-6 LC-PUFA VO, n-6 LC-PUFA FO (P<0.05) 2.3 NKA 1,, VO NKA FO (P<0.05) FO LC-PUFA (EPA DHA) NKA, VO FO, 10 NKA (P<0.05), NKA (P>0.05)

954 22 表 4 实验饲料与各处理组黄斑蓝子鱼鳃磷脂脂肪酸组成 ( 面积百分比 ) Tab.

5 表 4 实验饲料与各处理组黄斑蓝子鱼鳃磷脂脂肪酸组成 ( 面积百分比 ) Tab. 4 Main fatty acids composition of phospholipid in experimental diets and gill of Siganus canaliculatus at different treatments (%area) n=3; x ±SE treatment dietary group VO FO VO FO VO FO VO FO C18: 2n ±0.02 a 0.09±0.01 b 0.1±0.01 ab 0.04±0.01 c 0.11±0.03 ab 0.02±0.01 c C18: 3n ±0.02 a 0.16±0.00 ab 0.20±0.04 ab 0.10±0.00 ab 0.18±0.06 ab 0.09±0.02 b C20: 2n6 / ±0.02 a 0.10±0.00 b 0.23±0.02 a 0.09±0.01 b 0.27±0.05 a 0.09±0.01 b C20: 3n6 / ±0.02 a 0.18±0.01 b 0.21±0.01 ab 0.12±0.00 c 0.20±0.01 ab 0.12±0.01 c C20: 4n6 / ± ± ± ± ± ±0.02 C18: 3n ±1.18 a 0.76±0.08 c 8.73±0.41 b 0.59±0.01 c 7.09±0.29 b 0.60±0.09 c C18: 4n3 / ±0.32 a 0.28±0.05 d 1.66±0.04 b 0.20±0.00 d 1.16±0.03 c 0.15±0.02 d C20: 3n3 / ±0.01 b 0.16±0.03 a 0.08±0.01 b 0.08±0.01 b 0.06±0.00 b 0.08±0.01 b C20: 5n3 / ±0.01 b 0.67±0.02 a 0.38±0.03 b 0.48±0.01 ab 0.41±0.01 b 0.50±0.03 ab C22: 5n3 / ± ± ± ± ± ±0.02 C22: 6n3 / ±0.19 c 10.55±0.22 a 6.00±0.28 d 8.25±0.09 b 5.20±0.15 d 7.65±0.02 b SFA ±2.73 c 75.51±0.65 a 64.71±1.48 bc 73.36±5.22 ab 64.20±1.10 bc 77.12±1.54 a MUFA ±0.30 ab 11.17±0.85 b 13.79±0.94 a 10.61±0.62 b 15.66±1.66 a 12.76±0.47 b n-6 C18PUFA ±0.04 a 0.25±0.01 ab 0.30±0.03 a 0.14±0.01 b 0.30±0.09 a 0.11±0.01 b n-3 C18PUFA ±1.50 a 1.04±0.13 d 10.39±0.37 b 0.80±0.02 e 8.25±0.16 c 0.75±0.07 e n-6 LC-PUFA / ±0.01 a 0.89±0.04 c 1.17±0.02 b 0.72±0.05 d 1.16±0.03 b 0.74±0.01 d n-3 LC-PUFA / ±0.23 c 11.71±0.26 a 6.64±0.28 d 8.96±0.21 b 5.92±0.13 d 8.47±0.01 b : (P<0.05). Note: Values within the same row with different letters are significantly different (P<0.05). 1 Na + /K + -ATPase (P<0.05). Fig. 1 Enzyme activity of Na + /K + -ATPase in gills of Siganus canaliculatus at different treatment Different letters show significant differences between different groups (P<0.05). 2.4 NKA mrna 2, NKA mrna, VO NKA 2 Na + /K + -ATPase mrna (P<0.05). Fig. 2 mrna expression of Na + /K + -ATPase relative to β-actin in gills of Siganus canaliculatus at different treatments. Different letters show significant differences between different groups (P<0.05). mrna FO (P<0.05), VO FO, 10 NKA mrna

6 5 : LC-PUFA (P<0.05), NKA mrna 3 NKA, [18],, 10%~20%, [19],, , NKA [20 21],, NKA 10, 10 NKA, NKA (Galaxias maculates), NKA α-1b, NKA α-1a [4, 22] Tang [3] 鳉 (Oryzias latipes) 鳉 (Oryzias dancena) NKA,, NKA α, 20 32, NKA mrna 10, NKA,, NKA [7, 23], NKA,, NKA mrna, n-3 LC-PUFA, [9],, 10 n-3 LC-PUFA 20 32, n-3 LC-PUFA, LC-PUFA [14] [13, 15] (Chirostoma estor), LC-PUFA, LC-PUFA, DHA [24], (Oncoryhnchus masou) PUFA,, PUFA, EPA DHA [25 26],, LC- PUFA, LC-PUFA [27 28],, LC-PUFA, LC-PUFA, [11],, II LC- PUFA [14], LC-PUFA,,, [29] Nano [12] LC-PUFA Caco-2,, LC-PUFA Bell [30],,, DHA EPA,,, LC-PUFA FO n-3 LC- PUFA VO, LC-PUFA (Litopenaeus vannamei) n-3 LC-PUFA [31], EPA DHA [32 34], LC-PUFA

7 n-3 LC-PUFA, EPA DHA, ARA [35] [36] (Scylla serrata) [37], ARA, ARA ( ) ARA, ARA,, ARA [35 36],, LC-PUFA, ARA ARA 鮨 (Morone saxatilis) ARA, [38] [39] (Paralichthys dentatus) [40], ARA Atalah [8] EPA DHA, ARA (Dicentrarchus labrax), ARA, DHA EPA NKA Ca 2+ - ATPase, [41 42], LC-PUFA (Oreochromis mossambicus) [43] [31], ARA LC-PUFA,, VO n-6 LC-PUFA FO, LC-PUFA LC-PUFA ARA, [44] ARA [45], NKA mrna Van Anholt [9] ARA NKA, Carrier [10] ARA,, ARA NKA, VO n-6 LC-PUFA FO, NKA FO, n-6 LC-PUFA NKA, LC-PUFA NKA, NKA, n-3 LC-PUFA n-6 LC-PUFA n-3 LC-PUFA, n-6 LC-PUFA NKA, [46], NKA,, LC-PUFA, n-3 LC-PUFA,, ; n-6 LC-PUFA NKA, 参考文献 : [1] Evans D H, Piermarini P M, Choe K P. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste[j]. Physiol Rev, 2005, 85(1): [2] Kang C K, Tsai S C, Lee T H, et al. Differential expression of branchial Na + /K + -ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater[j]. Comp Biochem Physiol A, 2008, 151(4): [3] Tang C H, Lai D Y, Lee T H. Effects of salinity acclimation on Na + /K + ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica)[j]. Comp Biochem Physiol A, 2012, 163(3 4): [4] Urbina M A, Schulte P M, Bystriansky J S, et al. Differential expression of Na +, K + -ATPase α-1 isoforms during seawater acclimation in the amphidromous galaxiid fish Galaxias maculatus[j]. J Comp Physiol B, 2013, 183(3): [5] Flores A M, Shrimpton J M. Differential physiological and endocrine responses of rainbow trout, Oncorhynchus mykiss, transferred from fresh water to ion-poor or salt water[j]. Gen

8 5 : LC-PUFA 957 Comp Endocrinol, 2012, 175(2): [6] Lin Y M, Chen C N, Lee T H. The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brackish water and fresh water[j]. Comp Biochem Physiol A, 2003, 135(3): [7] Kang C K, Liu F C, Chang W B, et al. Effects of low environmental salinity on the cellular profiles and expression of Na +, K + -ATPase and Na +, K +, 2Cl cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus[j]. Fish Physiol Biochem, 2012, 38(3): [8] Atalah E, Hernández-Cruz C M, Ganuza E, et al. Importance of dietary arachidonic acid for the growth, survival and stress resistance of larval European sea bass (Dicentrarchus labrax) fed high dietary docosahexaenoic and eicosapentaenoic acids[j]. Aquacult Res, 2011, 42(9): [9] Van Anholt R D, Koven W M, Lutzky S, et al. Dietary supplementation with arachidonic acid alters the stress response of gilthead seabream (Sparus aurata) larvae[j]. Aquaculture, 2004, 238(1 4): [10] Carrier J K, Watanabe W O, Harel M, et al. Effects of dietary arachidonic acid on larval performance, fatty acid profiles, stress resistance, and expression of Na + /K + ATPase mrna in black sea bass Centropristis striata[j]. Aquaculture, 2011, 319(1 2): [11] Yehuda S, Rabinovitz S, Carasso R L, et al. The role of polyunsaturated fatty acids in restoring the aging neuronal membrane[j]. Neurobiol Aging, 2002, 23(5): [12] Nano J L, Nobili C, Girard-Pipau F, et al. Effects of fatty acids on the growth of Caco-2 cells[j]. Prostagl Leuk Ess Fatty Acids, 2003, 69(4): [13] Zheng X Z, Torstensen B E, Tocher D R, et al. Environmental and dietary influences on highly unsaturated fatty acid biosynthesis and expression of fatty acyl desaturase and elongase genes in liver of Atlantic salmon (Salmo salar)[j]. Biochim Biophys Acta-Mol Cell Biol Lipids, 2005, 1734(1): [14] Li Y Y, Hu C B, Zheng Y J, et al. The effects of dietary fatty acids on liver fatty acid composition and Δ 6 -desaturase expression differ with ambient salinities in Siganus canaliculatus[j]. Comp Biochem Physiol B, 2008, 151(2): [15] Sarker M A A, Yamamoto Y, Haga Y, et al. Influences of low salinity and dietary fatty acids on fatty acid composition and fatty acid desaturase and elongase expression in red sea bream Pagrus major[j]. Fish Sci, 2011, 77(3): [16] Li Y Y, Chen W Z, Sun Z W, et al. Effects of n-3 HUFA content in broodstock diet on spawning performance and fatty acid composition of eggs and larvae in Plectorhynchus cinctus[j]. Aquaculture, 2005, 245(1 4): [17] Whelan J A, Russell N B, Whelan M A. A method for absolute quantification of cdna using real-time PCR[J]. J Immunol Meth, 2003, 278: [18] Hiroi J, McCormick S D. New insight into gill ionocyte and ion transporter function in euryhaline and diadromous fish[j]. Respir Physiol Neurobiol 2012, 184(3): [19] Hwang P P, Lee T H, Lin L Y. Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms[j]. Am J Physiol-Regul Integr Comp Physiol, 2011, 301(1): R28 R47. [20] Tzeng W N, Lizuka Y, Shiao J C, et al. Identification and growth rate comparison of divergent migratory contingents of Japanese eel (Anguilla japonica)[j]. Aquaculture, 2003, 216(1 4): [21] Luo M Z, Guan R Z, Jin H. Effects of salinity on Na+/K+-ATPase activity in gill and kidney of Anguilla marmorata and A. bicolor pacifica elver[j]. Oceanologia et Limnologia Sinica, 2013, 44(3): [,,. (Anguilla marmorata) (A. bicolor pacifica) Na + /K + -ATP [J]., 2013, 44: ] [22] Bystriansky J S, Richards J G, Schulte P M, et al. Reciprocal expression of gill Na + /K + -ATPase α-subunit isoforms α1a and α1b during seawater acclimation of three salmonid fishes that vary in their salinity tolerance[j]. J Exp Biol, 2006, 209(10): [23] Hwang P P, Lee T H. New insights into fish ion regulation and mitochondrion-rich cells[j]. Comp Biochem Phys A, 2007, 148(3): [24] Fonseca-Madrigal J, Pineda-Delgado D, Martínez-Palacios C, et al. Effect of salinity on the biosynthesis of n-3 long-chain polyunsaturated fatty acids in silverside Chirostoma estor[j]. Fish Phsiol Biochem, 2012, 38(4): [25] Sheridan M A, Allen W V, Kerstetter T H. Changes in the fatty acid composition of steelhead trout (Salmo gairdnerii Richardson), associated with parr-smolt transformation[j]. Comp Biochem Phys B, 1985, 80(4): [26] Li H O, Yamada J. Changes of the fatty acid composition in smolts of masu salmon (Oncorhynchus masou), associated with desmoltification and sea-water transfer[j]. Comp Biochem Phys A, 1992, 103(1): [27] Bell J G, Tocher D R, Farndale B M, et al. The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation[j]. Lipids, 1997, 32(5):

9 [28] Tocher D R, Bell J G, Dick J R, et al. Polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr smolt transformation and the effects of dietary linseed and rapeseed oils[j]. Fish Physiol Biochem, 2000, 23(1): [29] Alava V R. Effect of salinity, dietary lipid source and level on growth of milkfish (Chanos chanos) fry[j]. Aquaculture, 1998, 167(3 4): [30] Bell J G, Farndale B M, Dick J R, et al. Modification of membrane fatty acid composition, eicosanoid production, and phospholipase a activity in Atlantic Salmon (Salmo salar) gill and kidney by dietary lipid[j]. Lipids, 1996, 31(11): [31] Hurtado M A, Racotta I S, Civera R, et al. Effect of hypoand hypersaline conditions on osmolality and Na + /K + -ATPase activity in juvenile shrimp (Litopenaeus vannamei) fed low- and high-hufa diets[j]. Comp Biochem Phys A, 2007, 147(3): [32] Watanabe T, Arakawa T, Takeuchi T, et al. Comparison between eicosapentaenoic and docosahexaenoic acids in terms of essential fatty acid efficiency in juvenile striped jack Pseudocaranx dentex[j]. Nippon Suisan Gakk, 1989, 55(11): [33] Watanabe T. Importance of docosahexaenoic acid in marine larval fish[j]. J World Aquacult Soc, 1993, 24(2): [34] Rainuzzo J R, Reitan K I, Olsen Y. The significance of lipids at early stages of marine fish: a review[j]. Aquaculture, 1997, 155(1 4): [35] Moya-Falcón C, Hvattum E, Dyrøy E, et al. Effects of 3-thia fatty acids on feed intake, growth, tissue fatty acid composition, β-oxidation and Na +, K + -ATPase activity in Atlantic salmon[j]. Comp Biochem Physiol B, 2004, 139(4): [36] Hurtado M A, Racotta I S, Arjona O, et al. Effect of hypoand hyper-saline conditions on osmolarity and fatty acid composition of juvenile shrimp Litopenaeus vannamei (Boone, 1931) fed low- and high-hufa diets[j]. Aquacult Res, 2006, 37(13): [37] Romano N, Wu X G, Zeng C S, et al. Growth, osmoregulatory responses and changes to the lipid and fatty acid composition of organs from the mud crab, Scylla serrata, over a broad salinity range[j]. Mar Biol Res, 2014, 10(5): [38] Harel M, Gavasso S, Leshin J, et al. The effect of tissue docosahexaenoic and arachidonic acids levels on hypersaline tolerance and leucocyte composition in striped bass (Morone saxatilis) larvae[j]. Fish Physiol Biochem, 2001, 24(2): [39] Koven W, Barr Y, Lutzky S, et al. The effect of dietary arachidonic acid (20: 4n 6) on growth, survival and resistance to handling stress in gilthead seabream (Sparus aurata) larvae[j]. Aquaculture, 2001, 193(1 2): [40] Willey S, Bengtson D A, Harel M. Arachidonic acid requirements in larval summer flounder, Paralichthys dentatus[j]. Aquacult Int, 2003, 11(1 2): [41] Blaustein M P, Goldman W F, Fontana G, et al. Physiological roles of the sodium-calcium exchanger in nerve and muscle[j]. Ann NY Acad Sci, 1991, 639: [42] Taffet G E, Pham T T, Bick D L M, et al. The calcium uptake of the rat heart sarcoplasmic reticulum is altered by dietary lipid[j]. J Membrane Biol, 1993, 131(1): [43] Van Anholt R D, Spanings F A T, Koven W M, et al. Dietary supplementation with arachidonic acid in tilapia (Oreochromis mossambicus) reveals physiological effects not mediated by prostaglandins[j]. Gen Comp Endocr, 2004, 139(3): [44] Sargent J R, Tocher D R, Bell J G. The Lipids. Fish Nutrition[M]. 3rd ed. San Diego: Academic Press, [45] Lands W E M. Biosynthesis of prostaglandins[j]. Annu Rev Nutr, 1991, 11: [46] Kaplan J H. Biochemistry of Na, K-ATPase[J]. Annu Rev Biochem, 2002, 71:

10 5 : LC-PUFA 959 Research on relationship between LC-PUFA biosynthesis and osmoregulation in Siganus canaliculatus XIE Dizhi 1, 2, XU Shude 1, 3, CHEN Fang 1, 2, WANG Shuqi 1, YOU Cuihong 1, LI Yuanyou 1 1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou , China; 2. College of Fisheries, Henan Normal University, Xinxiang , China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai , China Abstract: To explore the relationship between osmoregulation and long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis in rabbitfish (Siganus canaliculatus), an 8-week feeding trial was performed in juvenile rabbitfish with diets that contained fish oil (FO) or a blend of vegetable oils (VO; perilla and canola oils) at three salinities: 10, 20, and 32. The growth performance, fatty acid composition of gill phospholipids, and activity and expression levels of gill Na + /K + ATPase (NKA) under different treatments were analyzed. The results showed that, at the same salinity, there was no effect of dietary lipid source on growth performance (P>0.05), whereas n-3 LC-PUFA content in gill phospholipids in the FO dietary groups was significantly higher than those in VO treatments (P<0.05). In particular, although the contents of n-6 LC-PUFA in VO-fed fish were lower than in FO-fed fish, the opposite was observed for n-6 LC-PUFA contents. Furthermore, the activity and mrna expression levels of gill NKA in VO groups were significantly higher than those in FO groups (P<0.05). Under different salinities, the growth indexes at a salinity of 10 were significantly lower than those at salinities of 20 and 32 in both dietary groups (P<0.05), whereas the LC-PUFA contents, and activity and mrna expression levels of NKA in fish fed VO were significantly higher than that in fish fed VO (P<0.05). In addition, there were no significant differences in either of the higher two salinities treatments (P>0.05). The results indicate that a salinity of 10 may not suitable for rabbitfish culture. Furthermore, FO-derived diets can improve n-3 LC-PUFA content in gill phospholipids, which helps maintain membrane lipid fluidity and osmotic balance. Alternatively, VO-derived diets can regulate osmotic pressure by synthesizing n-6 LC-PUFA to up-regulate NKA expression and activity. Key words: Siganus canaliculatus; salinity; lipid sources; Na + /K + ATPase; osmoregulation; LC-PUFA biosynthesis Corresponding author: Li Yuanyou. yyli@stu.edu.cn

60 12 ( Pampus argenteus), [ 1 ], [ [ 2 ],, ( ) cm, ( ),, g] 1. 2,,, 50%d , ph 7. 9 [ 3 ], 0. 5, 6 8 mgl - 1, 27, 24 h [

60 12 ( Pampus argenteus), [ 1 ], [ [ 2 ],, ( ) cm, ( ),, g] 1. 2,,, 50%d , ph 7. 9 [ 3 ], 0. 5, 6 8 mgl - 1, 27, 24 h [ 12 2 2 0 1 6 4 South China Fisheries Science Vol. 12, No. 2 Apr., 2016 doi: 10. 3969/ j. issn. 2095-0780. 2016. 02. 009 1 1, 2 1 1 1,,,, ( 1.,, 200090; 2., 201306) : ( Pampus argenteus), 3 22, 27 32, 27,