4 5 Vol. 4 No. 5 2013 10 Journal of Food Safety and Quality Oct., 2013 王 超 1, 周勇 2, 杜冰 1* (1., 510640; 2. ( ), 529156) 摘要 : 目的 方法 SD,, (Hb) (FEP) 结果 4,, (P<0.05), FEP (P<0.01);, (P<0.05), FEP (P<0.01), ; 结论 关键词 : ; ; ; ; Evaluation on extracts from Lentinula edodes and Auricularia auricula-judae in improving nutritional anemia in rats WANG Chao 1, ZHOU Yong 2, DU Bing 1* (1. College of Food Science, South China Agricultural University, Guangzhou 510640, China; 2. Infinitus(China) Co., Ltd., Jiangmen 529156, China) ABSTRACT: Objective To study the effect of extracts from Lentinula edodes (LE) and Auricularia auricula-judae (AA) on improving nutritional anemia. Methods The SD rats were made into nutritional anemia model by feeding iron deficient diet, then the rats were randomly divided into low iron model group, LE extract gavage group, AA extract gavage group, ferrous lactate control group and normal control group, and the body weight, food intake, content of hemoglobin (Hb), red blood cell free protoporphyrin (FEP) content, and changes of hematocrit of each group were analyzed. Results After 4 weeks, when compared with low iron model group, the rats of LE group and AA group had significant increases (P<0.05) in body weight, food intake, hemoglobin content and hematocrit, while having significant decreases (P<0.01) in FEP content. When compared with ferrous lactate group, the rats of middle and high dose group had significant increases (P< 0.05) in hemoglobin content and hematocrit and significant decreases (P<0.01) in FEP content. In addition, the body weight, food intake increased slightly. Besides, the indicators of extract gavage group were close to normal group. Conclusion The LE and AA extracts could significantly improve the symptoms of nutritional anemia rats. KEY WORDS: Lentinula edodes; Auricularia auricula-judae; extraction; nutritional anemia; rats * 通讯作者 :,, E-mail: gzdubing@163.com *Corresponding author: DU Bing, Associate Professor, College of Food Science, South China Agricultural University, No.483, Wushan Road, Tianhe District, Guangzhou 510642, China. E-mail: gzdubing@163.com
1388 4,, D,, [1,2],, [3-5], [6,7], [8,9],,, [10],,,,, [11],,,, 1 材料与方法 1.1 受试物与受试对象 :, ( ) (, 1: 25, 6 h, 70 [12,13] ) : SD, SPF, 40~60 g, 1.2 仪器和试剂, : Milli-Q/B( Millipore); Mek-7222k ( ); BS-3000A ( ); ELX800 ( BIO-TEX); FT-630ǐ- ( ) : (FEP)ELISA ( ); ( ); ( ) 1.3 实验方法 : 10, ; 80, [14] : (Hb)<100 g/l, Hb A A A B B B, 10 / [15], A B 1 ml (A B), 2 ml(a B), 3 ml (A B) (, 1.5 ml), 3 ml; 3 ml (, 1.5 ml); 3 ml (, 1.5 ml);, 30 d 1.4 观察及检测指标 1.4.1 体态及行为, 1.4.2 体重, 1 1.4.3 摄食量, 1, g / d -1, (g/ d -1 )=[ (g) (g)]/ ( ) [16,17] 1.4.4 血红蛋白含量, 10 μl, 2.5 ml,,, 15 min,, 1.4.5 红细胞内游离原卟啉含量, 4,, 2%, 4, : (4:1), 10 min, 0.5 mol/l,, ELISA 1.4.6 红细胞压积 9 μl,,, 2 min, [18]
5, : 1389 1.5 统计分析 Hb ( x ±, SPSS 13.0 2 结果与分析 2.1 受试样品 A B 对缺铁性贫血大鼠模型日常的影响, ( ),,, A B 2.2 受试样品 A B 对缺铁性贫血大鼠模型体重的影响 1,,,,, (P<0.01) 2.3 受试样品 A B 对治疗期间缺铁性贫血大鼠模型摄食量影响 2,,,, ;, A, ; 表 1 A B 对治疗期间缺铁性贫血大鼠模型体重的影响 ( x± Table 1 Effect of A, B on body weight of iron deficiency anemia rat model during treatment ( x± (g/ ) n ( ) 1 2 3 4 10 163.8 17.9** 201.8 15.4** 218.4 16.9** 239.6 20.2** 252.7 20.5** 10 140.7 25.1 166.0 27.6 187.8 24.3 196.7 23.8 204.3 21.5 10 142.4 21.4 177.5 24.0 202.1 24.2* 219.6 21.2* 244.5 19.9** A 10 140.6 18.9 177.7 20.2 205.7 23.0* 221.1 25.1** 240.6 27.7** A 10 144.5 17.6 184.7 20.5* 203.2 22.4* 220.6 21.6** 240.4 28.0** A 10 147.8 19.4 186.1 18.7* 210.7 20.1* 226.8 21.8** 252.6 22.5** B 10 143.3 20.9 185.8 19.5* 207.1 19.9* 226.0 13.3** 247.9 15.5** B 10 144.0 21.6 179.6 18.5* 204.9 16.3* 220.1 17.0** 243.2 22.0** B 10 143.6 12.5 183.2 12.4* 208.5 15.4* 222.6 14.9** 248.5 16.0** 表 2 A B 对治疗期间缺铁性贫血大鼠模型摄食量的影响 ( x± Table 2 Effect of A, B on food intake of iron deficiency anemia rat model during treatment ( x± n ( ) (g/ ) 1 2 3 4 10 24.4 0.7** 24.5 0.6** 23.7 0.1** 23.8 3.7** 10 16.4 2.1 16.7 1.0 17.1 2.9 17.5 2.4 10 16.0 0.4 16.5 1.0 18.3 2.4 19.7 0.8* A 10 17.0 2.9 17.0 0.7 17.2 2.1 18.4 1.7 A 10 17.9 0.1 18.8 1.0* 18.4 3.5 18.0 0.8 A 10 16.9 0.6 19.0 2.9* 19.0 0.9 20.5 3.7* B 10 17.3 2.7 17.9 2.5 17.6 2.4 20.0 3.0* B 10 17.6 0.1 18.2 0.3* 20.8 0.8* 21.0 0.7** B 10 16.5 1.1 18.6 2.1* 19.7 1.5 22.5 1.4**
1390 4 2.4 受试样品 A B 对缺铁性贫血大鼠模型血红蛋白含量 红细胞内游离原卟啉值的影响 3,, (P<0.01),,, Hb (P<0.01), FEP A 2.5 受试样品 A B 对缺铁性贫血大鼠模型红细胞压积的影响 4,, (P<0.01),,, 表 3 A B 对缺铁性贫血大鼠模型血红蛋白含量和 FEP 的影响 ( x± Table 3 Effect of A, B on content of hemoglobin and FEP of iron deficiency anemia rat model during treatment ( x± (g/l) n ( ) Hb Hb FEP (μg/dl) 10 133.5 6.8** 164.0 5.2** 45.6 11.9** 10 82.4 8.0 118.1 10.7 67.3 12.7 10 82.7 7.3 150.8 4.8** 44.8 9.3** A 10 82.3 6.7 139.7 6.2** 61.8 17.0* A 10 82.8 6.9 148.0 4.1** 50.5 16.9** A 10 82.1 7.8 150.6 2.8** 42.7 12.3** B 10 82.5 7.0 153.6 7.4** 63.4 19.8* B 10 82.6 7.2 155.0 4.7** 54.3 13.6** B 10 82.2 6.9 156.4 6.8** 44.2 15.2** 表 4 A B 对缺铁性贫血大鼠模型红细胞压积的影响 ( x± Table 4 Effect of A, B on hematocrit of iron deficiency anemia rat model during treatment ( x± (%) n ( ) 10 27.2 4.3** 28.9 2.0** 10 22.1 2.1 24.0 3.2 10 22.4 2.3 27.6 3.0** A 10 21.8 3.4 25.8 4.3* A 10 22.5 4.1 28.4 2.2** A 10 23.4 4.2* 30.0 3.2** B 10 21.4 3.2 25.9 1.6* B 10 22.1 2.8 27.4 2.8** B 10 24.5 3.4* 29.8 3.8**
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