PCR PCR 1996 Applied Biosystems PCR PCR PCR. PCR PCR PCR PCR 1. PCR Ct C Cycle t threshold Ct 1 1. Ct 2. threshold PCR 15 3-15 10 threshold = 10 SD cycle 6-15 3. Ct Ct 1 Ct Ct 2 Ct 2. 4. PCR 2 1 TaqMan PCR PCR Taq 5 3
DNA PCR 3 3. TaqMan 2 SYBR
PCR SYBR SYBR DNA SYBR PCR 1 PCR 3 1 PCR PCR 4 2 PCR 5 3 PCR ELISA PCR ELISA 6 2 PCR PCR 96 PCR 96 4 4. PCR 96 Ct PCR 1 PCR PCR Ct PCR
1 Ct PCR Ct Ct Ct 4 2 Ct Ct PCR 2 PCR PCR PCR 7,8 Texas 9 Applied Biosystems 7700 PCR PCR PCR 10,11,12,13 1. HBV 3 () 1 3 1 4 HBV 80 90 HBV 80?00 HBV HBV 1 100 2. (1)
(2) 60 6 6 3 69 2 75 HBV-DNA HBV-DNA HBV-DNA 10pg 10ul HBIG HBV HBeAg 85 HBeAg 31 -HBe 12 3 HBV 1 32 1 128 1 256 1 512 HBV 22.2 50 65.2 88.8 95 30 (3) HB3Ag HBV (4) ( )-
HBV 100 44 4 56 4 61 9-76 37.63 20nm - - - (5) HBV 3. (ADCC) HBV-DNA Tc HBV (1) 2 3 3 1 3 1 (2) (3) 10 10
( ) 171umol L 4.? (1) ( ) HBV-DNA (2) ALT AST A G TBI( ) ALP( ) GGT(Y? (3) B 5.? (1) 2?/FONT>3 2 6 3?/FONT>4 6 29?/FONT>43 (18?/FONT>84 ) (2) HBsAG 1?/FONT>7 ( 4 ) 65 98 (3)
1?/FONT>6 20 1?/FONT>4 1?/FONT>12 6 (4) 28.1 4.5? (1) (2) (3) H8sAg 1 ( HBsAb HBeAg HBeAb HBcAg HBcAb) HBV-DNA 6.? HBV-DNA DNA HBV-DNA HBeAg HBcAg DNA () HBV HBV DNA ( 1 ) 7. 4 (1) ( -HBs)
(2) (3) HbsAg -HBc HBeAg (4) 8.?? -HBs 100 10 20 -HBs HBV -HBs -HBs 1 2 -HBs 10 -HBs -HBs 4-5 -HBs 6 3 -HBs 2 -HBs -HBs -HBs ( -HBs) o 9. HBs?
(HBIG) -HBs 5 10 -HBs HBcAg -HBc -HBs -HBs -HBc -HBs -HBs-IgM 10. HBsAb HBV-DNA? HBsAb 10 HBV NA HBsAb HBsAb ( ) HBV HBV NA HBV a, a HBV HBV S HBsAb HBsAb o 11.? 2 12.? ( )
(1) -HBs (2) 6?2 (3) (4) HBs -HBs ; (5) 85 5 ( ) 5 10 1 4 1 (1) DNA DNA DNA (2) HBV NA (3)HBV (4)HBV DNA 12 43 1 11 5.4 13. e HBeAg HBcAb
HbeAb HbcAb 14.? 1 2 (1 16 1 8 ) 10 HBeAg 40?0% 15. e 1. 2. 3. 4. Ara-AMP 5. 6. a1 16. ALT ( ) HBV HBV-DNA 50 6?2 24.
ALT 30 40 HBV DNA HBeAg 3 HBeAg HBV DNA 44.4 45.3 1 10 25. PCR PCR 100 0.01fg 10 12mg 2.5 PCR PCR PCR PCR 10 15 PCR PCR 26.