2014 Vol.16 No.1 茁 琢 1. 550002 2. 550003 LPS IL-1 茁 IL-6 TNF- 琢 50 SD 5 20 滋 g kg -1 LPS 30 min Ti 240 min 驻 T 驻 T=T i -T 0 1 ELISA IL-1 茁 IL-6 TNF- 琢 3h 3.5 h 4 h 3 P<0.01 P< 0.05 3.5 h 4 h P<0.01 P<0.05 3 IL-1 茁 P<0.05 P<0.01 IL-6 TNF- 琢 P< 0.05 LPS IL-1 茁 IL-6 TNF- 琢 doi: 10.11842/wst.2014.01.010 R285.5 A 1 3 1.1 SPF SD 200 依 20 g SCXK 2012-0005 Lipopolysaccharides LPS -1 茁 Interleukin -1 茁 IL-1 茁 -6 1.2 Bupleurum Interleukin -6 IL -6- 琢 Tumor Necrosis factor- 琢 TNF- 琢 chinense DC. Scutellaria baicalensis Georgi.[ Codonopsis pilosula Franch. Nannf. ] [ Pinellia ternata Thunb. Breit. 2013-02-21 2013-03-06 ] Glycyrrhiza uralensis 元 2010 2010297 元元 58
Fisch. Zingiber officinale Rosc. Ziziphus jujube Mill. 芃 3 40 g 15 g 15 g 15 g 15 g 15 g 4 10 g 800 ml 1 h 30 min 800 ml 30 min 100 ml 1.30 g ml -1 15 g 1.00 g ml -1 6g 0.91 g ml -1 3 4 益 6 IL-1 茁 IL-6 TNF- 琢 ELISA R&D 201211 LPS Escherichca coli endotox-in 055: B5 Sigma L2880 :1010A031 20 滋 g ml -1 1.3 MC-246 32 耀 42 益 依 0.1 益 TDL-5A Bio -TEK ELX808 BioTeck 2 2.1 70 20 依 2 益 50% 耀 60% 12 h 3d 3d 2cm 2 10 h 30 min 2 2 >0.3 益 2 T 0 50 5 10 2.2 20 滋 g kg -1 20 滋 g ml -1 LPS 13 g kg -1 10 g kg -1 9.1 g kg -1 30 min T i 240 min 驻 T 驻 T=T i -T 0 1 10% 5 耀 10 min 5 ml 4 h 4 益 3 000 rpm 15 min-20 益 ELISA IL-1 茁 IL-6 TNF- 琢 2.3 SPSS 17.0 for Windows 依 x 依 s P<0.05 P<0.01 3 3.1 20 滋 g kg -1 LPS 驻 T LPS 3h P<0.01 4h 3 耀 4 h 3 P<0.01 P<0.05 3.5 h 4 h P<0.01 P<0.05 1 3.2 20 滋 g kg -1 LPS 4 h IL- 1 茁 IL-6 TNF- 琢 3 IL-1 茁 P<0.05 P<0.01 IL-6 TNF- 琢 P<0.05 IL-1 茁 P< 0.01 IL-1 茁 TNF- 琢 59
2014 Vol.16 No.1 IL-1 茁 P<0.05 3 IL-6 TNF- 琢 2 4 [1] [2] LPS [3] LPS 20 滋 g kg -1 2.5 耀 5.5 h 4 h [4] 38.5 益 1.15 益 20 滋 g kg -1 LPS 4h LPS LPS [5 耀 7] IL-1 茁 IL-6 [8] [9] TNF- 琢 [5] IL-6 TNF- 琢 IL-1 茁 IL-1 茁 IL-6 TNF- 琢 IL-1 茁 IL-6 TNF- 琢 12 耀 15 g 6 耀 12 g 3 耀 6 g 10 耀 30 g [2] 1 LPS 20 滋 g kg -1 驻 T x 依 s n=10 0.5h 1h 1.5h 2h 2.5h 3h 3.5h 4h 0.20 依 0.42 0.13 依 0.30 0.15 依 0.41 0.30 依 0.31 0.33 依 0.38 0.22 依 0.35 0.16 依 0.20 0.12 依 0.23 0.35 依 0.31 0.40 依 0.44 0.46 依 0.45 0.61 依 0.60 0.63 依 0.54 吟 0.74 依 0.30 1.03 依 0.69 1.11 依 0.45 0.22 依 0.15 0.18 依 0.24 0.17 依 0.23 0.32 依 0.32 0.53 依 0.18 0.40 依 0.38 0.30 依 0.33 0.40 依 0.28 0.31 依 0.15 0.37 依 0.25 0.44 依 0.20 0.63 依 0.25 0.54 依 0.17 0.55 依 0.38 吟 0.68 依 0.36 吟 0.71 依 0.29 0.33 依 0.21 0.39 依 0.33 0.46 依 0.30 0.63 依 0.28 0.58 依 0.19 0.59 依 0.34 0.83 依 0.34 0.85 依 0.34 吟吟吟 P 约 0.01 P 约 0.01 P 约 0.01 2 LPS 20 滋 g kg -1 4h IL-1 茁 IL-6 TNF- 琢 x 依 s n=10 IL-1 茁 /ng L -1 IL-6/ng L -1 TNF- 琢 /ng L -1 34.56 依 14.16 吟 230.63 依 33.93 448.09 依 125.83 74.29 依 20.86 吟 341.87 依 68.26 吟 651.22 依 123.22 43.75 依 13.34 284.73 依 39.79 528.54 依 129.55 53.89 依 18.68 327.79 依 48.60 620.98 依 110.41 56.65 依 18.24 吟 331.39 依 43.69 634.27 依 115.42 P 约 0.01 P 约 0.01 吟 吟吟 P 约 0.01 60
[10] LPS LPS LPS IL-1 茁 IL-6 TNF- 琢 [11] 1,..,2006,19(12) 颐 42~44. 2,..,2003,5(4) 颐 311~313. 3,..,2008,18(4) 颐 567~578. 4,,. LPS.,2003,37(10) 颐 44~47. 5 Heida J G, Mosh S L, Quentin J. The role of interleukin-1 茁 in febrile seizures. Brain Dev, 2009, 31(5) 颐 388~393. 6 Nur B G, Sahinturk D, Coskun M, et al. Single nucleotide polymor 原 phism and production of IL -1 茁 and IL -10 cytokines in febrile seizures. Neuropediatrics, 2012, 43(4) 颐 197~199. 7 Yu H M, Liu W H, He X H, et al. IL-1 茁 : animportant cytokine asso 原 ciated with febrile seizures? Neurosci Bull, 2012, 28(3) 颐 306~307. 8 Dzhndoian Z T, Martirosian N G. The role of aldosterone and IL-6 in the pathogenesis of inflammation in familial Mediterranean fever. Georgian Med News, 2012, 20(6) 颐 44~48. 9 Dundar M, Kiraz A, Balta B, et al. TheroleofTNF- 琢 andpai-1 gene polymorphisms in familial Mediterranean fever. Mod Rheumatol, 2013, 23(1) 颐 140~145. 10,,,..,2002,24(8) 颐 627~628. 11,,,..,2012,31(18) 颐 31~32. Effect of Xiao-Chai-Hu Decoction with Different Doses of Bupleurum on Body Temperature and Content of IL-1 茁,IL-6,TNF- 琢 in Serum Induced by LPS among Fever Rat Models Cao Feng 1,TangAmei 2 (1. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China; 2. The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, China) Abstract: This study was aimed to observe the effect of Xiao-Chai-Hu Decoction (XCHD) with different doses of Bupleurum on body temperature and content of IL-1 茁,IL-6andTNF- 琢 in serum among fever rat models induced by lipopolysaccharide (LPS) in order to and investigate its action mechanism. A total of 50 rats were randomly divid 鄄 ed into 5 groups, including the normal control group, fever model group, XCHD group (high-dose), XCHD group (middle-dose), and XCHD group (low-dose). The fever rat model was established by intraperitoneal injection of LPS (20 滋 g/kg). At the same time, all rats were given corresponding XCHD or saline solution by gavage. The body tem 鄄 perature of rats (Ti) was measured every 30 min for 240 min after the injection of LPS. Change values of body tem 鄄 perature 驻 T( 驻 T=T i -T 0 ) were calculated. After the last measurement of body temperature, blood was taken for the de 鄄 tection of IL-1 茁, IL-6 and TNF- 琢 level in serum with ELISA. The results showed that the body temperature of XCHD group (high-dose) was obviously decreased at the time point of 3 h, 3.5 h, 4 h (P <0.01,orP <0.05).This temperaturereducingeffectatthetimepointof3.5hand4hwasbetterthanthe XCHD group (middle-dose) and XCHD group (low-dose, P <0.01,orP < 0.05). Compared with the fever model group, all three XCHD groups can 61
2014 Vol.16 No.1 obviously reduce the IL-1 茁 level (P < 0.05). Among them, the XCHD group (high-dose) had obvious effect (P < 0.01). Compared with the fever model group, the XCHD group (high-dose) can obviously reduce contents of IL-6 and TNF- 琢 in serum (P < 0.05). It was concluded that XCHD can reduce fever induced by LPS in rats and decrease contents of IL-1 茁, IL-6 and TNF- 琢 in serum. This effect has a positive dose-effect relationship with the dose ofbu 鄄 pleurum in a certain range. Keywords: Dose of Bupleurum, Xiao-Chai-Hu Decoction, lipopolysaccharide, antifebrile 62