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1 Chinese Journal of Applied Entomology 2016, 53(2): DOI: /j.issn * 北京和河北地区小菜蛾的抗药性动态高雪 2** 杨家强 1 徐宝云 1 杨峰山 2*** 吴青君摘要目的 Plutella xylostella L. 方法结果 Bt 结论关键词 1*** Insecticide resistance in diamondback moth populations in Beijing and Hebei GAO Xue 2** YANG Jia-Qiang 1 XU Bao-Yun 1 YANG Feng-Shan 2*** WU Qing-Jun 1*** (1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing , China; 2. College of Life Science, University of Heilongjiang, Harbin , China) Abstract [Objectives] To clarify the level of insecticide resistance in the diamondback moth (DBM) in Beijing and Hebei and thereby provide information to improve control of this pest. [Methods] The resistance of the DBM to nine insecticides was evaluated from 2011 to 2015 using the leaf-dipping method. [Results] Populations of DBM collected from Beijing and Zhangjiakou, Hebei Province, were susceptible to chlorantraniliprole, spinosad, and diafenthiuron. Resistance to Bacillus thuringiensis (Bt), chlorfenapyr and indoxacarb was moderate in some years, but populations were generally susceptible to these pesticides. Resistance to abamectin and chlorfluazuron was consistently moderate, and high resistance to beta-cypermethrin was found. [Conclusion] Beta-cypermethrin is not suitable for controlling DBM in Beijing and Hebei. The use of abamectin should be discontinued, either permanently or temporarily, to prevent the development of resistance to this pesticide. Other insecticides should be used in rotation, or in alternative ways. Key words Plutella xylostella, insecticide resistance, resistance monitoring, control Plutella xylostella (L.) 40 Furlong et al Talekar and Shelton, * Supported projects BAD19B06 blvt-15 ** First author gaoxuex@foxmail.com *** Corresponding author yangfshan@126.com wuqingjun@caas.cn Received Accepted

2 280 Chinese Journal of Applied Entomology 材料与方法 1.1 供试昆虫 3 5% Chlorantraniliprole10%Chlorfenapyr 2.5% Spinosad5%Indoxacarb 2% Abamectin3%Bt Bacillus thuringiensis5%chlorfluazuron20% Diafenthiuron 20% Beta-cypermethrin9 1.2 方法 Liu et al % Triton % Triton cm 10 s 6.5 cm % L.. D= h 72 h 10% 1.3 数据分析 POLO LeOra Software Inc., California, USA LC 50 95% Resistance ratio, RR = LC 50 / LC 结果与分析 2.1 北京地区小菜蛾对 9 种药剂的抗药性水平 Bt 2015 LC 河北张家口地区小菜蛾对 9 种药剂的抗药性水平 Bt 2014

3 2 : 281 Insecticide 表 1 北京地区小菜蛾田间种群对不同杀虫剂的抗药性水平 ( ) Table 1 Resistance of the filed population of Plutella xylostella to different insecticides in Beijing ( ) Year LC 50 (mg/l) 95% 95% confidence interval ±SE Chi square RR 5% ( ) 1.90± Chlorantraniliprole ( ) 1.27± ( ) 1.99± ( ) 1.71± ( ) 1.22± % ( ) 1.24± Chlorfenapyr ( ) 1.37± ( ) 1.56± ( ) 1.17± ( ) 1.38± % ( ) 1.57± Spinosad ( ) 1.61± ( ) 1.31± ( ) 1.36± ( ) 1.42± % ( ) 1.18± Indoxacarb ( ) 1.18± ( ) 1.47± (( ) 1.52± ( ) 1.37± % ( ) 1.40± Abamectin ( ) 1.20± ( ) 1.52± ( ) 1.15± ( ) 1.28± %Bt ( ) 1.39± Bacillus ( ) 1.37± thuringiensis ( ) 1.22± ( ) 1.20± ( ) 1.03± % ( ) 1.03± Chlorfluazuron ( ) 0.80± ( ) 0.86± ( ) 1.55± ( ) 0.96± % ( ) 1.45± Diafenthiuron ( ) 1.35± ( ) 0.95±

4 282 Chinese Journal of Applied Entomology 53 Insecticide Year LC 50 (mg/l) 95% 95% confidence interval ±SE 续表 1(Table 1 continued) Chi square RR ( ) 1,12± ( ) 1.44± % ( ) 1.30± Beta-cypermethrin ( ) 1.29± ( ) 1.41± ( ) 1.67± ( ) 1.65± Insecticide 表 2 河北张家口地区小菜蛾田间种群对不同杀虫剂的抗药性水平 ( ) Table 2 Resistance of the filed population of Plutella xylostella to different insecticides in Zhangjiakou, Hebei ( ) Year LC 50 (mg/l) 95% 95% confidence interval ±SE Chi square RR 5% ( ) 1.17± Chlorantraniliprole ( ) 2.03± ( ) 1.70± % ( ) 1.48± Chlorfenapyr ( ) 1.17± ( ) 1.17± % ( ) 0.99± Spinosad ( ) 1.16± ( ) 1.36± % ( ) 1.25± Indoxacarb ( ) 0.87± ( ) 1.52± % ( ) 1.06± Abamectin ( ) 0.83± ( ) 1.15± %Bt ( ) 0.87± Bacillus ( ) 1.46± thuringiensis ( ) 1.20± % ( ) 1.40± Chlorfluazuron ( ) 1.29± ( ) 1.55± % ( ) 1.18± Diafenthiuron ( ) 1.56± ( ) 1,12± % ( ) 1.43± Beta-cypermethrin ( ) 1.06± ( ) 1.67±

5 2 : 讨论 Bt Yang et al ~ Pu et al Lahm et al Bentley et al Dow Agro Sciences Sparks et al 参考文献 (References) Bentley KS, Fletcher JL, Woodward MD, Chlorantraniliprole: an insecticide of the anthranilic diamide class// Hayes Handbook of Pesticide Toxicology. Third Edition. London: Academic Press Furlong MJ, Wright DJ, Dosdall LM, Diamondback moth ecology and management: problems, progress and prospects. Annu. Rev. Entomol., 58(1): Fu W, Wei J, Wang QJ, Xu ZD, Sun Y, Yin L, Study on resistance of Plutella xylostella in different areas in Hunan Province to insecticides. Chinese Journal of Applied Entomology, 49(2): [,,,,,, , 49(2): ] Feng X, Li ZY, Wu QJ, Chen AD, Wu YD, Hou YM, He YR, Li JH, Xie SH, Zhang JM, Fu W, Ma CS, Research progress of the resistance management and sustainable control of diamondback moth (Plutella xylostella) in China. Chinese Journal of Applied Entomology, 48(2): [,,,,,,,,,,,, , 48(2): ] Liu F, Shi XZ, Liang YP, Wu QJ, Xu BY, Xie W, Wang SL, Zhang YJ, Liu NN, A 36-bp deletion in the alpha subunit of glutamate-gated chloride channel contributes to abamectin resistance in Plutella xylostella. Entomol. Exp. Appl., 153(2): Lahm GP, Cordova D, Barry JD, New and selective ryanodine receptor activators for insect control. Bioorg. Med. Chem., 17(12): Pu X, Yang YH, Wu SW, Wu YD, Characterization of abamectin resistance in a field-evolved multiresistant population of Plutella xylostella. Pest Manag. Sci., 66(4): Sparks TC, Thompson GD, Kirst HA, Hertlein MB, Larson LL, Worden TV, Thibault ST, Biological activity of the spinosyns, new fermentation derived insect control agents, on tobacco budworm (Lepidoptera: Noctuidae) larvae. J. Econ. Entomol., 91(6), Shao ZR, Feng X, Zhang S, Li ZY, Huang JD, Chen HY, Hu ZD, Guideline for insecticide resistance monitoring of Plutella xylostella (L.) on cruciferous vegetables. Beijing: China Agriculture Press. NY/T [,,,,,,, :. NY/T ] Talekar NS, Shelton AM, Biology, ecology and management

6 284 Chinese Journal of Applied Entomology 53 of the diamondback moth. Annu. Rev. Entomol., 38(1): Xia YM, Lu YH, Zhu X, Shen J, You H, Li JH, Determination of Plutella xylostella (L.) resistance to nine insecticides in central China. China Vegetables, (22): [,,,,,, , 22: ] Yin F, Feng X, Li ZY, Hu ZD, Lin QS, Chen HY, Resistance of diamondback moth to chlorantraniliprole in different areas. Plant Protection, 41(5): [,,,,,, , 41(5): ] Yang JQ, Tian LX, Xu BY, Xie W, Wang SL, Zhang YJ, Wang XJ, Wu QJ, Insight into the migration routes of Plutella xylostella in China using mt COI and ISSR markers. PloS ONE, 10(6): e You MS, Wei H, Research of Diamondback Moth. Beijing: China Agriculture Press [,, : ] Yin YQ, Zhao XQ, Li XY, Chen AD, The relationship between susceptibility of Plutella xylostella to insecticides and resistance. Chinese Journal of Applied Entomology, 48(2): [,,,, , 48(2): ] Yin YQ, Mu WD, Li XY, Zhao XQ, Huang CF, Ai Y, Chen AD, Resistance monitoring of diamondback moth and field control efficacy of pesticides against the pest in Tonghai, Yunnan Provice. Plant Protection, 41(3): [,,,,,,, , 41(3): ]

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