44 of the rats were treated with DMBA by intragastric administration at a dose of 2mg / kg weight to establish the experimental animal models of induc

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1 CHINESE JOURNAL OF COMPARATIVE MEDICINE June 2012 Vol. 22 No. 6 檵檵檵檵檵檵檵檵檵檵 Wistar BD BDC FS FSC 12 DMBA 2mg / kg BDC FSC 21 FS FSC 2 4 FS vs BD P = ** FSC vs BDC P < ** FS vs BD P = * FSC vs BDC P = * FS vs BD P = * FSC vs BDC P < * FS FSC P = ** P = * P = * DMBA R R R332 A doi / j. issn Study of the Flaxseed Lignan on the Animal Model of Breast Cancer Prevention and the Relation with the Body Estrogen LIU Shan 1 WANG Xiaobing 1 LI Aidong 2 LIU Zhaoyang 1 TIAN Haimei 1 LI Mo 1 ZHAO Wenya 1 LI Yanfen 1 LIU Jing 1 SONG Peipei 1 ZHANG Wei 1 1. Detection Center of Tumor Biology Cancer Institute &Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China 2. Central Laboratory Cancer Institute &Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China Abstract Objective To explore the function of flaxseed powder lingans on the prevention of breast cancer and the relation with estrogen on the ovariectomized female rats. Methods Foutyeight female wistar rat were randomly divided into basal diet group BD basal diet castrate group BDC flaxseed group FS and flaxseed castrate group FSC All candy_show@ yeah. net zhangww1954@ 126. com

2 44 of the rats were treated with DMBA by intragastric administration at a dose of 2mg / kg weight to establish the experimental animal models of induced breast cancer and the BDC and FSC groups were operated for the ovariectomy. On the 21 week the volume and weight of the palpable tumors were measured and conducted pathological examination by pathologist. Results The experimental animals were generally in well situation there was no obvious toxic or side effect in experimental groups palpable tumors appeared two to four weeks later in FS and FSC groups than that in the control Compared with the control groups the incidence and the average number of lesions of breast hyperplasia atypical hyperplasia breast cancer were significantly lower in flaxseed groups hyperplasis FS vs BD P = ** FSC vs BDC P < ** atypical hyperplasia FS vs BD p = * FSC vs BDC P < * breast cancer FS vs BD P = * FSC vs BDC P = * additionally the volume and weight of the tumors were lower in flaxseed groups. The results also suggested that the ability of inhibiting the hyperplasia and tumor cell growth of the flaxseed lignans is related with estrogen states hyperplasis P = ** atypical hyperplasia P = * Breast cancer P = *. Conclusions Flaxseed powder lignans can effectively prevent and reduce the occurrence of breast cancer precancerous lesions and hyperplasia induced by DMBA and the preventive effect of flaxseed powder lignans are affected by body estrogen. The above results may be of reference value for screening effective population and preventing breast cancer by lignans. Key words Lingans Flax seed Rat Breast cancer 8g 40 ml 75% 100 ml OLYMPUS BH ~ 24 50% ~ 60% mg /100 g Wistar g SCXK SYXK basal diet BD basal diet 5 d castrate BDC flax seed FS flax seed castrate FSC Dimethylbenz α anthracene 3% 50 mg / kg BD FS DMBA sigma 80 1g DMBA 2 ml ml mg / ml 10% 6 PBS 8% 10% 24 h

3 45 10 HE 1 BDC BD = / 100% = / = 2 1 / 100% = / 100% = 1 Tab. 1 Average body weight of rats in each group / Group N g Body weight g = BDC ± / 100% = / 100% = / 2. 2 = 6 2 / 100% 1. 6 SPSS16. 0 x 珋 ± s P < P < Microsoft Excel Group 2 Tab FS FSC 15 BD ± FSC ± FS ± Comparation of palpable tumor weights and volumes / Rats number with tumor / group rats number g Average tumor weight g mm 3 Average tumor volume mm 3 BDC 6 / ± ± BD 5 / ± ± FSC 1 / FS 0 /10

4 46 3 FS FSC 1 DMBA 3 Fig. 1 Impaction of Flax seed powder and castrated to DMBAinduced surface palpable breast tumor growth Tab. 3 3 DMBA The incidence of DMBAinduced mammary hyperplasia mammary atypical hyperplasia and mammary carcinoma in rats Group BD BDC FS Group number No. of bearing rats Incidence % Medium No. of Hyperplasia hyperplasia bearing rats Inhibitory rate % Incidence % Atypical hyperplasia P No. of bearing rats Medium No. of atypical hyperplasia bearing rats Inhibitory rate % Incidence % Medium No. of tumor Carcinoma each rat bearing FSC vs BD vs FSC ** vs BD ** vs FSC < ** vs BDC P No. of bearing rats Inhibitory rate % * P < ** P < Note * P < ** P < vs BD vs FSC * vs BD * vs FSC * vs BDC P vs BD vs FSC * vs BD * vs FSC * vs BDC

5 47 IARC GLOBOCAN % 46 14% Jianmin C Jasdeep KS Paul C et al. Flaxseed and pure 0. 9% ~ 1. 5% 66 secoisolariciresinol diglucoside but not flaxseed hull reduce human breast tumor growth MCF7 in athymic mice J. 100 ~ 800 Nutrition And Disease Katharina B Aida KZ Alina V et al. Metaanalyses of lignans Nutr from DMBAinduced mammary carcinoma J rats J 6 Zhou YJ Liu YE Cao JG et al. Wistar 7 8 J. Int J Cancer Susan EM Paola M Stephen B et al. Cancer Biol Med stems. Plant Physiol Dong K Pu JX Zhang HY et al and enterolignans in relation to breast cancer risk J. Am J Clin 3 Louis WC Mary NC Wings TL et al. A rat cell line derived Life Sciences 4 Cecile VS Pierre D Christe P et al. Phytoestrogen rich diets modulate expression of brca1 and brca2 tumor suppressor genes in mammary glands of female Wistar rats J. Cancer Research 5 Masao H Atsuko M Nobuyuki I et al. Effects of dietary perila oil soybean oil and safflower oil on 7 12dimethylbenz a antfhracene DMBA and 1 2dimethylydrazine DMH induced mammary gland and colon carcinogenesis in female SD. Carcinogenesis Vitexins naturederived lignan compounds induce apoptosis and suppress tumor growth J. Clin Cancer Res Dal ML Zucchetto A Talamini R et al. Effect of obesity and other lifestyle factors on mortality in women with breast cancer Dietary lignan intakes and risk of preand postmenopausal breast cancer J. Int J 9 Anazi AF Qureshi VF Arbieri C et al. Preventive effects of phytoestrogens against postmenopausal osteoporosis as compared to the available therapeutic choices an overview J. J Nat Sci 10 Huis R Morreel K Fliniaux O et al. Natural hypolignification is associated with extensive oligolignol accumulation in flax Lignans from Kadsura polysperma and Their Antineurodegenerative Activities J. J Nat Prod

6 刘珊, 亚麻籽木酚素预防乳腺癌及与雌激素的关系 ( 正文见第 43 页 ) LIU%Shan,%et%al.%Study%of%the%Flaxseed%Lingan%on%the%Animal%Model%of%Breast%Cancer%Prevention%and% %%%%%the%relation%with%the%body%estrogen %(Full%text%on%page%43) A B C D E F G H 图 2 正常乳腺导管 HE 400(A) 和 HE 800(B); 单纯性增生 HE 200(C) 和 HE 800(D); 不典型增生 HE 200(E) 和 HE 800(F); 浸润性乳腺癌 HE 200(G) 和 HE 800(H) Fig. 2 Usual%breast%duct%HE 400(A)%and%HE 800(B);%Usual%ductal%hyperplasia%HE 400(C)%and%HE 800(D);% Ductal%intraepithelial%neoplasia%HE 400(E)%and%HE 800(F);%Ifiltrative%carcinoma%HE 400(G)%and%HE 800(H)

TGF-β AngⅡ B SD ~ 220g SPF. SCXK No SYXK ~ 25 40% ~ 70% OR37G-C

TGF-β AngⅡ B SD ~ 220g SPF. SCXK No SYXK ~ 25 40% ~ 70% OR37G-C 2017 7 43 4 Journal of Southwest Minzu University Natural Science Edition Jul. 2017 Vol. 43 No. 4 doi10. 11920 /xnmdzk. 2017. 04. 008 1 1 1 2 1 1 3 1. 610212 2. 6100413. 610031 AngⅡ TGF-β. SD 60 180 ~