5 : Hyphantria cunea Drury Prodenia litura Fabricius Lymantria dispar L. 1.2 饲料配方的正交试验设计及实施 Estigmene acrea Drury Bombyx mori

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Chinese Journal of Applied Entomology 2014, 51(5): 1376 1386. DOI: 10.7679/j.issn.2095 1353.2014.165 适合三种鳞翅目昆虫的一种人工饲料配方 * 曹利军杨帆唐思莹陈敏 ** 100083 摘要目的方法 Hyphantria cunea Drury Prodenia litura Fabricius Lymantria dispar L.

1 Chinese Journal of Applied Entomology 2014, 51(5): DOI: /j.issn 适合三种鳞翅目昆虫的一种人工饲料配方 * 曹利军杨帆唐思莹陈敏 ** 摘要目的方法 Hyphantria cunea Drury Prodenia litura Fabricius Lymantria dispar L. 3 结果 12% 2% 4.5% 1.5% 0.05% 1.0% 0.4% 0.25% 0.5% B 0.003% 0.1% 0.035% 结论 3 关键词 Development of an artificial diet for three lepidopteran insects CAO Li-Jun YANG Fan TANG Si-Ying CHEN Min ** (Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing , China Abstract [Objectives] The establishment of insect colonies under artificial conditions is important for research and the development of a set of artificial diets suitable for a variety of insect species can greatly decrease costs and improve the efficiency of maintaining such colonies. [Methods] A diet was developed using two rounds of orthogonal design methods and employed successfully in rearing three Noctuoidea species: Hyphantria cunea (Drury), Prodenia litura (Fabricius) and Lymantria dispar (L.). [Results] The diet contained wheat germ (12%), sugar (2%), casein (4.5%), agar (1.5%), choline chloride (0.05%), Wesson s salt (1.0%), sorbic acid (0.4%), methyl p-hydroxybenzoate (0.25%), ascorbic acid (0.5%), Vanderzant s vitamin mix (0.003%) cholesterol (0.1%) and inositol (0.035%). [Conclusion] The three insect species reared on this diet displayed normal larval development, pupal weight, pupation and eclosion rates. This research will provide a basis for the development of artificial diets for other polyphagous insects. Key words artificial diet, orthogonal design, Hyphantria cunea, Prodenia litura, Lymantria dispar 1979 Singh and Moore 1985) * YX AA ** minch@bjfu.edu.cn

2 5 : Hyphantria cunea Drury Prodenia litura Fabricius Lymantria dispar L. 1.2 饲料配方的正交试验设计及实施 Estigmene acrea Drury Bombyx mori L. Mamestra brassicae L. Plutella xylostella L Tryporyza incertulas Walker 1 1 Mythimna separate Walker 材料与方法 1.1 试验材料 Morus alba L. Quercus mongolica Fischer ex Ledebour KCl 120 g CuSO g KI 0.05 g MnSO g NaCl 105 g MgSO 4 90 g NaF 0.57 g K2Al SO4 2 H2O 0.09 g KH2PO4 31 g 2 L 1 CaCO g FePO4 4H2O g Ca3 PO g 2 1 g 2 ml g 2 B 0.5 g 0.25 g 50 ml 1 mol/l KOH 1 g 1 g 0.25 g 0.25 g 0.02 g B g 100 ml g B 100 μl 11 3 L L 饲料配制 a B b a 70 b

3 1378 Chinese Journal of Applied Entomology 51 4 Table 1 表 1 第 1 次正交试验的营养因素与水平 Nutrition factors and their levels for the 1st orthogonal design Level A (%) B (%) C (%) D (%) E (%) F (%) G (%) H (%) I (%) J (%) K (%) L (%) A: B C D E F G H B I J K L A. Wheat germ; B. Sugar; C. Casein; D. Wesson s salt; E. Sorbic acid; F. Methyl p-hydroxybenzoate; G. Ascorbic acid; H. Vanderzant s vitamin mix; I. Agar; J. Cholesterol; K. Choline chloride; L. Inositol. The same below. Table 2 表 2 第 2 次正交试验的营养因素与水平 Nutrition factors and their levels for the 2nd orthogonal design Level D (%) E (%) F (%) G (%) H (%) J (%) L (%) 饲养方法 3 cm 5 cm 4 cm 80 1 cm 10% 1 h h 25 70%~80% L 80 3 cm 10% 1.5 优化配方的检验与评价 2 Hypercompe Lymantridae Noctuidae 3 2 结果与分析 2.1 第 1 次正交试验结果与分析 d 27

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5 Chinese Journal of Applied Entomology 2014, 51(5): DOI: /j.issn * YX AA ** minch@bjfu.edu.cn

6 5期曹利军等: 适合三种鳞翅目昆虫的一种人工饲料配方 1381

7 1382 Chinese Journal of Applied Entomology 51

8 5 : 1383 * * 优化配方的评价 B 第 2 次正交试验结果与分析 d * B 结论与讨论 20 d 6 Table 4 表 4 第 1 次正交试验各因素最佳水平出现的频次 Frequency of optimal level of each factor in the 1st orthogonal design Level A B C D E F G H I J K L 表 5 Table 5 第 1 次正交试验较优水平组合 Results of the 1st orthogonal design Nutrition factors (%) Percentage Nutrition factors (%) Percentage Nutrition factors (%) Percentage A 12 K 0.05 G B 2 D H C 4.5 E J I 1.5 F L

9 1384 Chinese Journal of Applied Entomology 51

10 5期曹利军等: 适合三种鳞翅目昆虫的一种人工饲料配方 1385

11 1386 Chinese Journal of Applied Entomology 51 Table 7 表 7 第 2 次正交试验各因素最佳水平出现的频次 Frequency of optimal level of each factor in the 2nd orthogonal design Level D E F G H J L Table 8 表 8 最优配方的组分及含量 Nutrition factors and their optimal levels Nutrition factors (%) Percentage Nutrition factors (%) Percentage Nutrition factors (%) Percentage A 12.0 K 0.05 G B 2.0 D 1.00 H C 4.5 E 0.40 J I 1.5 F 0.25 L ~19 d % 0.2%~0.4% d ~ Pachliopta aristolochiae Fabricius 3 3

12 5期曹利军等: 适合三种鳞翅目昆虫的一种人工饲料配方 1387

13 Chinese Journal of Applied Entomology 2014, 51(5): DOI: /j.issn , 3 参考文献 (References) Singh P, Moore RF, Handbook of Insect Rearing. Vol. I. Elsevier Science Publishers BV , , 30(6): ,,, , 37(6): [Chen QJ, Li GH, Pang Y, A simple artificial diet for mass rearing of some noctuid species. Chinese Bulletin of Entomology, 37(6): ],,,, , 24(4): [Fang j, Zhu L, Yang ZD, Zhao BG, Advances and problems in insect artificial diet studies. Journal of Sichuan Forestry Science and Technology, 24(4): ],, , 24(2): [Jiang SR, Fu FL, Effect of several meridic diets on armyworm Rearing. Journal of Sichuan Agricultural University, 24(2): ],,,,,, , 40(10): [Li GG, Lv SX, Yang X, Xu SL, Zheng CG, Chen QJ, Influence of the photoperiod on the artificial rearing, Sprodoptera litura (Fabricius). Guangdong Agricultural Sciences, 40(10): ],, :, 33(1): [Lin PQ, Liu FS, Comparision of mian quality indexes of Prodenia litura Fabr. Reared by both artificial and natural diets. Joural of Zhongshan University: Natural Science Edition, 33(1): ],,, , 7(2): [Lu WH, Tang ZQ, Zhang WQ, The research of semi-artificial diets of Prodenia litura Fabr. Huanan Agricultural University, 7(2): 33 42],,,,,, , CN A, [Lv BQ, Zhu WJ, Yan Z, Peng ZQ, Jin QA, Wen HB, An artificial diet of Prodenia litura Fabr., production method, and rearing method. China, CN A, ],,, , 29(4): [Mao JP, Gu WW, Pu GQ, The effects of different artificial diets on development of Prodenia litura Fabr. China Sericulture, 29(4): 27 29],,,, , 38(6): [Sui H, Li CY, Jiao SB, Zheng GL, The comparation of 4 artificial diets for rearing fall webworm. Shandong Forest Technology, 38(6): ],, , 22(1): [Tu YG, Zeng JH, A method for artificial rearing of common cutworm, spodoptera l itura. Acta Agriculturae Jiangxi, 22(1): ], : [Wang YN, Insect artificial diet book. Shanghai: Shanghai Science and Technology Press ],,, , 32(6): [Wang ZY, Wang XG, Wang LJ, Experiment on the feeding of lymantria dispar L.by artificial diet. Journal of Northeast Forestry University, 32(6): ],, : [Qi JL, Su MD The artifial diet of insect, spider, and mite. Beijing: Science Press ],,,,,, 2013.,., 34(7): [Yan Z, Lv BQ, Peng ZQ, Zhang CH, Jin QA, Wen HB, Effect of tomato, pepper and artificial diet on the growth, development and fecundity of spodo ptera litura fabricius. Chinese Journal of Tropical Crops, 34(7): ],, , 20(4): [Ying XL, Zeng L, The research of semi-artificial diet of Pachliopta aristolochiae Fabricius. * YX AA ** minch@bjfu.edu.cn

14 5 : 1389 Journal of Huanan Agricultural University, 20(4): 24 27],,,, , CN C, ZL [Zhang YA, Wang YZ, Xu BM, Qu JL, The artificial rearing and Hyphantria cunea (Drury) and artificial diet for larve. China, CN C, ZL ],, , 1(19): [Zhu JE, Jin ZY, On the antiseptics used to artifical feed for silkworm. Cereal & Feed Industry, 1(19): ],,,,, , 38(3): [Zhu LM, Ni JP, Cao XY, Huang CX, Cui Q, A method for artificially rearing to the cotton leafworm Prodenia litura Fabricius. Chinese Bulletin of Entomology, 38(3): ]

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