白藜芦醇对妊娠期糖尿病大鼠血糖及氧化 应激影响的研究 ( 河北省沧州市中心医院妇产二科沧州 061001) 摘 要 Resveratrol 100 5 SD Streptozotocin STZ 35 mg kg -1 6 4 30 60 120 240 mg kg -1 20 20 5 SD 20 0 7 14 2 Malondialdehyde MDA Superoxide Dismutase SOD Glutathione Peroxidase GSH-Px Catalase CAT MDA SOD GSH-Px CAT P>0.05 30 60 120 240 mg kg -1 MDA P<0.01 120 240 mg kg -1 P<0.01 SOD GSH-Px CAT P<0.01 关键词 doi 10.11842/wst.2015.10.029 R587.1 A Gestational Diabetes Mellitus, GDM [1] 6.6% [2] 2 [5-8] STZ 5 3-4 [3] 1 材料与方法 Lappas [4] M 2015-04-28 2015-04-28 1.1 实验动物 SD 200-240 g 2014101700051 2114
1303018 1.4 方法 23-1.4.1 动物分组与模型制备 25 65%-70% 12 h 1.2 主要药物及试剂 1 2 120917 98% 107948-305021 99.4% 1 5 STZ 35 mg kg -1 72 h 10940128 Sigma 13.5 mmol L -1 10950147 1.3 仪器 310 mg kg -1 4 [8] 20 UV-1200-20 5 SD 20 TDL-50C 2 1 8 100 6 30 60 120 240 图 1 白藜芦醇高效液相色谱图 ( 左图为试验药物, 右图为对照品 ) 表 1 白藜芦醇对妊娠期糖尿病大鼠空腹血糖水平的影响 ( /mg kg -1 0 /mmol L -1 7 /mmol L -1 14 /mmol L -1 5.8 0.6 5.9 0.9 5.7 0.8 6.1 0.8 6.3 0.5 6.2 0.7 16.4 2.5 16.2 2.7 16.1 2.4 30 16.3 2.5 15.9 2.3 15.7 2.7 P<0.01 60 16.5 2.2 15.3 2.5 14.9 2.2 120 16.2 2.4 13.6 2.2 12.7 1.9 240 16.3 1.9 12.1 1.8 9.6 1.6 2115
1.4.2 血糖和胰岛素水平检测 0 7 14 P<0.01 120 ELISA 240 mg kg -1 2 P<0.01 1 1.4.3 血清中 MDA 含量以及 SOD GSH-Px CAT 活性的检测 2 P<0.01 2 2 000 rpm 5 min 2.2 白藜芦醇对妊娠期糖尿病大鼠血清中 MDA 含 量及 SOD GSH-Px CAT 活性的影响 CAT 1.4.4 统计学方法 SPSS MDA P<0.01 17.0 t 60 120 240 mg kg -1 P<0.05 MDA P<0.01 P<0.01 120 240 mg kg -1 SOD GSH- 2 结果 2.1 白藜芦醇对妊娠期糖尿病大鼠空腹血糖和胰 岛素水平的影响 P<0.01 120 240 mg kg -1 2 MDA SOD GSH-Px CAT Px CAT 3 3 讨论 表 2 白藜芦醇对妊娠期糖尿病大鼠胰岛素水平的影响 ( /mg kg -1 0 /miu ml -1 7 /miu ml -1 14 /miu ml -1 13.8 2.1 14.0 1.9 13.9 2.2 13.2 2.4 13.3 2.2 13.2 2.5 5.5 0.9 5.5 1.0 5.6 1.0 30 5.6 1.1 6.1 1.2 6.6 1.3 P<0.01 60 5.5 1.0 6.7 1.5 7.1 1.4 120 5.4 0.8 7.6 1.4 8.5 1.6 240 5.4 1.0 10.5 1.8 12.1 1.7 表 3 白藜芦醇对妊娠期糖尿病大鼠血清中 MDA 含量的影响 ( /mg kg -1 MDA/nmol L -1 SOD/U mg -1 prot GSH-Px/U mg -1 prot CAT/U mg -1 prot 3.0 0.8 14.2 1.4 19.3 1.8 4.35 0.84 3.4 1.1 13.7 1.9 18.7 2.7 4.16 1.03 7.3 1.5 9.0 1.7 13.6 2.1 2.51 0.94 30 6.8 1.4 9.4 2.1 13.9 2.5 2.64 0.82 60 6.2 1.5 10.1 1.8 14.4 2.8 2.87 0.91 120 5.8 1.3 10.7 1.9 15.0 2.3 3.16 1.07 240 5.1 1.2 11.3 2.0 15.7 2.8 3.42 1.01 P<0.01 2116
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548-551. ( 92(4 2008, 14 Laumbach R J, Kipen H M, Ko S, et al. A controlled trial of acute effects of human exposure to traffic particles on pulmonary oxidative 45-51. 11 Toxicol. 2014, stress and heart rate variability. Part Fibre 15 Lartigue A, Burlat B, Coutard B, et al. The Megavirus chilensis Cu,Zn- Superoxide Dismutase: the first viral structure of a typical CCS- 824- ( 89(1 Virol. 2015, independent hyperstable dimeric enzyme. J 832. 16 Jin Y, Liu K, Peng J, et al. Rhizoma Dioscoreae Nipponicae polysaccharides protect HUVECs from H 2 O 2 -induced injury by regulating PPARγ factor and the NADPH oxidase/ros-nf-κb signal 149-158. ( 232(1 Lett, 2015, pathway. Toxicol 参考文献 Effects of Resveratrol on Plasma Glucose and Oxidative Stress Ability in Gestational Diabetic Rats Li Qin, Xing Baoheng, Cao Yalei, Dong Xiujuan (The Second Department of Obstetrics, Cangzhou Central Hospital, Cangzhou 061001, China) Abstract: This study was aimed to investigate the effects of resveratrol on plasma glucose and the oxidative stress ability in gestational diabetic rats. A total of 100 SD rats with gestation for 5 days were intraperitoneally injected with streptozotocin (STZ, 35 mg kg -1 ) to prepare gestational diabetic rat model. Rats were randomly divided into 6 groups, which were the normal gestation group, gestational diabetic model group, resveratrol (30, 60, 120 and 240 mg kg -1 ) treatment groups, with 20 rats in each group. A total of 20 rats with gestation for 5 days were selected as the normal gestation control group and another 20 rats with no gestation were selected as the normal control group. The levels of plasma glucose and insulin were determined on 0, 7, 14 days after experiment. Two weeks later, the contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in serum were determined. The results showed that compared with the normal control group, there were no significant difference on the levels of plasma glucose and insulin, the content of MDA, and the activities of SOD, GSH-Px and CAT of the normal gestation control group (P > 0.05). Compared with the gestational diabetic model group, the content of MDA in serum of the resveratrol (30, 60, 120 and 240 mg kg -1 ) treatment groups were significantly decreased (P < 0.05, P < 0.01); the level of plasma glucose were significantly decreased and the level of insulin was significantly increased of the resveratrol (120 and 240 mg kg -1 ) treatment groups (P < 0.05, P < 0.01); the activities of SOD, GSH-Px and CAT were also significantly increased (P < 0.05, P < 0.01). It was concluded that resveratrol had dose-dependent effect on reducing plasma glucose and improving antioxidant ability in gestational diabetic rats, which perhaps related to its effects on raising the level of insulin and improving the activity of antioxidant enzymes. Keywords: Resveratrol, gestational diabetes, oxidative stress, antioxidant 2118