46 3 Vol.46, No.3 2015 5 OCEANOLOGIA ET LIMNOLOGIA SINICA May, 2015 (Litopenaeus vannamei) G 0 * ( 524088) (Litopenaeus vannamei)7, 11, 270 (), Kung,, 11 270 13.52cm 31.05g, (P<0.05); (P<0.01), 0.903; t-, (P<0.05); 3, GH GH ZX ZX, GH GH SS SS ; G 1 1.927g, ; ; Kung ; ; Q953 doi: 10.11693/hyhz20141100316 (Litopenaeus vannamei), (White Prawn),,,, (, 2010),,,, 70%,,,, (, 2010),,,,, (, 2004),,,, (2009) ; (2011) (2013) 7,,,,, 11, 270 *, A201208B05 ;, 12239002-3, 14125007-2-9,, E-mail: 741164367@qq.com :,, E-mail: liujy70@126.com : 2014-11-10, : 2014-12-26
3 : (Litopenaeus vannamei) G 0 557, Kung, G 1,, 1 1.1 (Litopenaeus vannamei) 2013 5 6, 7, (KN) (SIS) (SS) 1 (ZK) 1 (ZX) 1 (GH) (HD) (YH), 2013 8 5 12,, 20 G 0 17, 11, KN KN SS SS GH GH HD HD ZX KN YH KN HD YH ZK YH ZX ZX KN HD HD SS 1.2, 150 11 17 m 2, ph, 5 10 15, 1 2 cm, 4000 10 m 2 30 d 1.3, 300, 5 6, (visible implant elastomer),,, 90 /m 2 270,, 30,,, 1.4 Excel, SPSS17.0 Kung (2013) (Z) : Z = y + C (y y ), y, y, C (C = 1 1/F, F F ) (P i ) : P i = å j 2 æ a ö ij k 0 1 - ç a çè 0 j ø, P i, k 0, a ij i j, a 0j j ( R ) (, 1995) : R = ( n å k j= 1 i s h j 2 ) / n, i k k, j j, h 2 [ Caballero-Zamora (2014), h 2 = 0.33], n 2 2.1 11 4 7 660, 1, 1 1 1, 11.37 16.66 cm, 12.37 15.00 cm, 0.47 1.06, 3.36% 7.76% 17.14 52.12 g, 11 24.06 42.73 g, 3.72 7.08, 12.22% 21.59%,,, ( 1),, (P<0.05), ( ),
558 46 表 1 凡纳滨对虾 270 日龄生长性状描述性统计量 Tab.1 Descriptive statistics of 270d growth traits of L. vannamei (cm) (g) (%) (%) KN HD 60 12.72 15.00 13.99 bc 0.47 3.36 21.97 41.26 32.60c 4.05 12.41 HD HD 60 11.62 14.52 13.13 e 0.61 4.62 18.74 38.67 28.20d 4.35 15.43 SS SS 60 12.60 15.53 14.10 b 0.75 5.32 20.96 50.19 34.52b 6.01 17.40 ZX KN 60 11.90 15.60 13.17 e 0.79 5.99 19.40 51.05 28.37d 6.13 21.59 YH KN 60 11.91 15.95 13.11 ef 0.80 6.10 19.76 52.12 28.31d 5.58 19.72 HD YH 60 11.37 14.01 12.37 g 0.59 4.78 18.40 35.45 24.06e 3.72 15.47 ZK YH 60 11.69 15.62 13.62 d 1.06 7.76 17.14 43.55 31.47c 6.78 21.55 KN KN 60 11.40 14.00 12.86 f 0.60 4.66 18.93 35.28 26.79d 3.83 14.31 GH GH 60 13.42 16.66 15.00 a 0.84 5.62 31.36 54.83 42.73a 7.08 16.57 HD SS 60 12.09 15.48 13.67 d 0.60 4.37 21.33 48.99 32.19c 4.45 13.83 ZX ZX 60 12.61 14.95 13.74 cd 0.49 3.57 23.30 42.17 32.49c 3.97 12.22 : (P<0.5) 1 270 Fig.1 Box plots of growth traits at 270d in L. vannamei 2.2,, (W) (L) 2, 0.894 0.903 0.903, (P<0.01) 表 2 凡纳滨对虾体质量与体长回归分析 Tab.2 Regression analysis of body weight and length in L. vannamei R 2 F df 1 df 2 Sig. Linear Y = 60.71883 + 6.78728X 0.894 5551.393 1 658 0.000 Quadratic Y = 38.26878 7.76915X + 0.53235X 2 0.903 3075.100 2 657 0.000 Cubic Y = 4.59971 0.41835X + 0.012788X 3 0.903 3072.000 2 657 0.000
3 : (Litopenaeus vannamei) G 0 559 2.3 2 (12.61 0.55) (15.63 0.39)cm, (12.41 0.43) (14.36 0.68)cm; 11 (26.08 3.48) (48.61 3.74)g (22.03 2.76) (30.44 3.41)g t-, 2 G 0 Fig.2 Analysis of gender differences in growth traits of L. vannamei 2.4 Kung 3 Kung 12.145 14.338 12.636 15.602, Kung 22.158 36.668 26.224 48.343 3 ( 4), GH GH ZX ZX, KN HD SS SS HD SS, ZK YH HD HD YH KN KN KN ZX KN HD YH ; GH GH SS SS, KN HD HD SS ZK YH, ZX KN ZX ZX YH KN HD HD KN KN HD YH 2.5,, 4 11 1.436 3.158g, 1.233 3.656g 1.927g, 1.927g
560 46 表 3 凡纳滨对虾 G 0 各交配组合生长性状 Kung 育种值和综合评定值 Tab.3 Kung values and comprehensive evaluation values of different mating combinations Kung sir dam sir dam KN HD 13.741 30.387 14.224 34.749 0.279 0.246 HD HD 12.809 25.556 13.471 30.948 0.115 0.136 SS SS 13.582 30.359 14.587 38.557 0.275 0.350 ZX KN 12.557 23.475 13.806 33.381 0.048 0.204 YH KN 12.549 24.667 13.682 32.059 0.083 0.168 HD YH 12.145 22.158 12.636 26.224 0.000 0.000 ZK YH 13.120 28.353 14.113 34.566 0.206 0.240 KN KN 12.420 24.046 13.317 29.695 0.062 0.101 GH GH 14.338 36.668 15.602 48.343 0.480 0.619 HD SS 13.383 29.585 13.946 34.758 0.248 0.241 ZX ZX 13.860 32.285 13.619 32.627 0.338 0.181 表 4 凡纳滨对虾 G 1 体质量性状选择反应预估 Tab.4 Selection response prediction of body weight traits in L. vannamei R dam sir P i dam sir GH GH 3.745 GH GH 4.069 30% 1.162 1.436 1.560 SS SS 4.686 ZX ZX 3.447 1.797 1.322 KN HD 3.464 KN HD 3.412 20% 1.402 1.603 1.579 HD SS 4.042 SS SS 4.055 1.870 1.876 ZK YH 5.818 HD SS 3.207 2.692 1.484 ZX KN 4.497 ZK YH 6.302 10% 1.758 2.609 3.656 ZX ZX 4.489 HD HD 3.305 2.604 1.917 YH KN 5.444 YH KN 2.299 3.158 1.334 HD HD 3.551 KN KN 2.353 2.060 1.365 KN KN 2.798 ZX KN 2.126 1.623 1.233 HD YH 3.478 HD YH 2.762 2.018 1.602 1.927 3 3.1, 2 5 :,, ;,,, ;, 1 1 1, (, 2013);,,,,,,, (Vela Avitúa et al, 2013), G 0,,,,
3 : (Litopenaeus vannamei) G 0 561 (2013) 6.28% 17.60% Andriantahina (2012) 18.26% Castillo-Juárez (2007) 10.6% 13.3% 12.22% 21.59%,,,, G 0 3.2, (Penaeus penicillatus) (P. chinensis) (P. monodon) (, 1996;, 2011;, 2011) (2006) W = 0.0059L 3.2974 (R 2 = 0.9940); (2006) 0.988, 0.903,,,,,,,,,, 3.3 ( ) (, 2009), (2012) (Cynoglossus semilaevis) ; (2014) (Fenneropenaeus chinensis),, 11, 3.4,, (, 2000), G 0, F 1, 1.927g (2013) (0.6 1.1),, (, 2008),,, 2013 BLUP,, BLUP,,,,, 2009.., 44(2): 141 146,, 2004.. :, 79 86,,, 1996.., 15(1) : 25 31,,, 2009.., 33(6): 901 911,,, 2013. Kung., 20(2): 308 315,,, 2012.., 36(9): 1331 1336,, 樑, 2006.., 24(6): 67 71,, 2000.., 24(3): 201 205,,, 2014.., 36(2): 116 112,,, 2008. BLUP., 29(3): 35 40,,, 2006.
562 46., 25(1): 23 26,,, 2013.., 37(12): 1770 1781,,, 2011.., 20(1): 63 70, 2011.. :, 35 43,,, 2013. (Litopenaeus vannamei)., 44(2): 445 452,,, 2011. 5., 38(4): 116 119,, 1995.. :, 16 26,,, 2010.., 27(6): 17 20 Andriantahina F, Liu X, Huang H et al, 2012. ZResponse to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei. Chinese Journal of Oceanology and Limnology, 30(2): 200 205 Caballero-Zamora A, Montaldo H H, Campos-Montes G R et al, 2014. Genetic parameters for body weight and survival in the Pacific White Shrimp Penaeus (Litopenaeus) vannamei affected by a White Spot Syndrome Virus (WSSV) natural outbreak. Aquaculture (in Press, http: //www.sciencedirect. com/science/article/pii/s0044848614004207#) Castillo-Juárez H, Casares J C Q, Campos-Montes G et al, 2007. Heritability for body weight at harvest size in the Pacific white shrimp, Penaeus (Litopenaeus) vannamei, from a multi-environment experiment using univariate and multivariate animal models. Aquaculture, 273(1): 42 49 Vela Avitúa S, Montaldo H H, M rquez Valdelamar L et al, 2013. Decline of genetic variability in a captive population of Pacific white shrimp Penaeus (Litopenaeus) vannamei using microsatellite and pedigree information. Electronic Journal of Biotechnology, 16(4): 1 10 GROWTH PERFORMANCE COMPARISON AND SELECTION EFFECT PREDICTION FOR SELECTIVE BREEDING G 0 GENERATION OF LITOPENAEUS VANNAMEI HU Zhi-Guo, LIU Jian-Yong, YUAN Rui-Peng, ZHANG Jia-Chen (Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China) Abstract Selective breeding is an effective way of healthy seeding and genetic improvement for Litopenaeus vannamei. We established 11 mating combinations of L. vannamei among 7 different genesis groups using inbreeding and hybridization. The growth performance for female and male among groups in 270 d experiment was compared and analyzed. The populations were selected and graded in the Kung value and comprehensive evaluation values. In addition, selection effect for body weight traits was predicted. The results show that these 11 populations of 270-day-old in age, the body length and body weight was 13.52 cm and 31.05 g. The among-population differences in body length and body weight were significant (P<0.05); the regression relationship between body weight and body length was extremely significant (P<0.01), and the regression coefficient reached 0.903. For each mating combination, t-test analysis was performed on body length and body weight, and the differences were significant or extremely significant. All the populations were assessed as excellent, good, and average levels according to the comprehensive evaluation value. Based on the above analyses, combinations of good sir and dam parents were selected. Moreover, the value of L. vannamei for breeding G 1 generation was 1.927 g predicted by comprehensive selection response. The results may provide a good reference for the next phase of L. vannamei family selection, and accelerate the process of the genetic improvement. Key words Litopenaeus vannamei; growth; Kung value; selection response; prediction