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1 8 6 Vol. 8 No Journal of Food Safety and Quality Jun., 2017 张谦筱 1,2, 安熙强 2, 白利平 3, 赵婷婷 2, 张涛 2, 郭君婷 2, 程江南 2, 兰卫 1*, 向小东 ( ; 2., ; 3., ; 4., , ) 4 摘要 : 目的 方法, Savege, 2, 结果 ; 80% 5% CMS-Na 结论, 关键词 : ; ; ; Antioxidant function of Brassica rapa L. polysaccharide and processing technology of its tablet ZHANG Qian-Xiao 1,2, AN Xi-Qiang 2, BAI Li-Ping 3, ZHAO Ting-Ting 2, ZHANG Tao 2, GUO Jun-Ting 2, CHENG Jiang-Nan 2, LAN Wei 1*, XIANG Xiao-Dong 4 (1. College of Traditional Chinese Medicine, Xinjiang Medical University, Urmuqi , China; 2. Food Safety Testing Department of Xinjiang Uygur Autonomous Region Medicine Research Institute, Urumqi , China; 3.Institute of Medicinal Biotechnology,Peking ,China; 4. Xinjiang Uygur Autonomous Region Keping County Shengquan Industrial Co., Ltd., Aksu , China) ABSTRACT: Objective To investigate the antioxidant function of Brassica rapa L. polysaccharide and processing technology of its tablet. Methods The crude polysaccharides of Brassica rapa L. were extracted by the method of water extraction and alcohol precipitation. The method of Savege was used to remove protein, and activated carbon was used to decolorize and purify the polysaccharides. Two groups of antioxidant experiments were carried out by using the extracted Brassica rapa L. polysaccharide. In order to determine the optimum molding process, the single factor method was used to select the adhesives, fillers, disintegrants and lubricants to prepare Brassica rapa L. polysaccharide tablets. Results Brassica rapa L. polysaccharide had good antioxidant activities. The optimum conditions of molding process were as follow: 80% ethanol was the adhesive, lactose was the filler, 5% dosage of CMS-Na was disintegrating agent and magnesium stearate was the lubricant. Conclusion Brassica rapa L. polysaccharide has good antioxidant activities, which lays the foundation for the development of natural immune 基金项目 : (KY ) Fund: Supported by Autonomous Region Public Welfare Scientific Research Institutes Basic Scientific Research Business Funded Projects (KY ) * 通讯作者 :,, qjfy_4318@qq.com *Corresponding author: LAN Wei, Professor, College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi , China. qjfy_4318@qq.com

2 regulator for further use of the traditional Uyghur medicine and food materials. KEY WORDS: Brassica rapa L.; polysaccharide; antioxidant; tablet 1 引言, (Brassica rapa L.),,, [1-4],, [5] [6] ; [7], (superoxide dismutase, SOD) [8] ;, [9] [10] [11], [12] [13-15] [16] [17],,, 1, (1,1-diphenyl-2-picrylhydrazyl, DPPH),,, 2 材料与方法 2.1 材料与试剂 (Brassica rapa L.) , , (Brassica rapa L.), (, ); ( ); (carboxymethyl starch sodium, CMC-Na) (, ); (lowly substituted hydroxypropylcellulose, L-HPC); (croscarmellose sodium, CCNa)(, ); (, ) 2.2 试验仪器 DFT-200 ( ); JM ( ); 101 ( ); FA2004N ( ); SCO82132 ( ); DZTW ( ); SHB-III ( ); LXJ-IIB ( ); DZKW6-6 ( ); YCD-EL259 ( ); AY-4 ( ); DZF ( ); UV765 ( ); TDP-5 ( ) ; YD-II ( ); ZBS-G9 ( ); CJY-300C ( ); FT-104B ( ); 3 1 ( ) [33] 2.3 维药恰玛古多糖提取 水提 20.0 g, 1:25(m:V), 500 ml,, 3 h, 3000 r/min 15 min,, 60 ; 500 ml 3 h, 3000 r/min 15 min,, 50 ml 醇沉, 94% 490 ml 80%, 4 (X) : C2 V X C1 C2 : V, ml;c 1, mol/l; C 2, % 12 h,,,, 60, 0.07 Pa,

3 6, : 除蛋白 5 mg/ml,, Savege 3 Savege ( : =1:3, V:V) 6 min,, 10 min, ; Savege, 脱色 60, 5%, min,, 50 ml, 490 ml, 80%, 4,,, 60, 0.07 Pa,,, 12%, 2.4 抗氧化性能力测定 DPPH 自由基清除能力 2 mg DPPH, 25 ml( 1 d), mol/l, 50% mg/ml 3, A B C, A DPPH 2 ml, 2 ml,, 30 min, 517 nm, A 1 ; B DPPH 2 ml 2 ml, A 2 ; C 2 ml 2 ml,, A 3 ; C(vitamin C, Vc) DPPH : 1 ( A1 A2) DPPH清除率 A3 : A 1 (DPPH+ ) ; A 2 + ; A 3 (DPPH+ ) 羟基自由基清除能力的测定 mg/ml 6, 18, 3, 9 mmol/l FeSO 4 1 ml 9 mmol/l - 1 ml, 2 ml, 0.5 ml 8.8 mmol/l H 2 O 2, min,, 510 nm,, 対, A 0 ;, A i ; H 2 O 2,, A j, Vc, : 羟基自由基清除率 ( A0 Ai ) A j : A 0 対 ; A i ( + ) ; A j ( + ) 2.5 恰玛古多糖片剂的制备,,, D- D- D- D-,, [19-24],, [25] 1000 g,,,, 0.5 g/, >80% 2015 [26,27], [27-32] 辅料筛选, [21,22] [23-25], 粘合剂筛选 50 g, 5%, 70% 75% 80% 85% 90%, 14 50,, 0.5%, 填充剂种类筛选 50 g,, 5% 80%, 14, 50,, 0.5%, 崩解剂种类及用量筛选 50 g, CMS-Na, L-HPC, CCNa 3,,, 润滑剂筛选 50 g, 0.5%,

4 2082 8, 2.6 片剂成型工艺考察指标 溶散时限 6,,,, 1, 6, [26] 硬度,,,, 40~100 N, 40~ 60 N [26] 脆碎度, 6.5 g,, 100,,,, 1%, [26] 3 结果与分析 3.1 恰玛古对 DPPH 自由基的清除作用 1, DPPH,, 5 mg/ml, DPPH 71.08%, 3.2 mg/ml 3.2 恰玛古多糖对羟基自由基的清除作用 2,,, 1 mg/ml, 76.61%, 0.7 mg/ml Fig. 1 1 Vc DPPH (n=3) Scavenging effects on DPPH free radica of Uygur medicine Brassica rapa L polysaccharide and Vc (n=3) : a ; b Vc Fig. 2 2 Vc (n=3) Scavenging effects on hydroxyl radical of Uygur medicine Brassica rapa L polysaccharide and Vc (n=3) : a ; b Vc

5 6, : 多糖片剂辅料的筛选 粘合剂的筛选 1,, 80% 填充剂种类筛选 2, 80%, 崩解剂的筛选 3, 2, CMC-Na 表 1 粘合剂的筛选 Table 1 Screening of adhesives (N) 70% / 75% 82 80% 65 85% 45 90% 39 表 2 填充剂的筛选 Table 2 Screening of fillers (N) (min) 20~ ~ ~75 39 : (1:1) 70~80 45 表 3 崩解剂的筛选 Table 3 Screening of disintegrants (%) (N) (min) CMS-Na L-HPC CCNa CMS-Na, 4, 5% 8%, 5% 8%,, 5% CMS-Na 表 4 崩解剂用量的筛选 Table 4 Screening of disintegrant dosage (%) (%) (N) (min) 润滑剂的筛选,, 5,,,, 2, 30,,, 表 5 润滑剂的筛选 Table 5 Screening of lubricants : - 4 结论与展望 2,,, DPPH,,,, 80% 5% CMS-Na

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