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1 4 5 Vol. 4 No Journal of Food Safety and Quality Oct., 2013 卢海燕, 王欣 *, 赵婷婷, 刘宝林 (, ) 摘要 :,,,, 关键词 : ; ; ; ; Research progress on the common detection methods and LF-NMR of illegal cooked oil LU Hai-Yan, WANG Xin *, ZHAO Ting-Ting, LIU Bao-Lin (Institute of Food Quality and Safety, University of Shanghai for Science & Technology, Shanghai , China) ABSTRACT: The illegal cooked oil is one of the most serious food safety issues in China, and currently there is no effective authentication method. In this paper, the current research progress of the discerning technologies for detection of illegal cooked oil, such as sensory test, physicochemical index, instrumental methods have been summarized, and the characteristics and related problems of these methods have been evaluated. The principle and application of low field nuclear magnetic resonance (LF-NMR) technology in the assessment of oil quality was specially introduced. All of these are designed to provide concrete references and research ideas for the identification of accurate and rapid test methods of the illegal cooked oil. KEY WORDS: illegal cooked oil; detection methods; characteristic substance; rapid detection; low field nuclear magnetic resonance (LF-NMR) 1 地沟油检测的重要性,, 基金项目 : (NSFC ) ( ) (11YZ109) Fund: Supported by the National Natural Science Foundation of China ( ), the Key Scientific and Technological Projects of Science and Technology Commission of Shanghai Municipality ( ), and Innovation Program of Shanghai Municipal Education Commission (11YZ109) * 通讯作者 :,,, wx @126.com *Corresponding author: WANG Xin, Associate Professor, Institute of Food Quality and Safety, University of Shanghai for Science & Technology, No.516, Jungong Road, Yangpu District, Shanghai , China. wx @126.com

2 5, : 1429, ;, [1], 2011,,,,,,,, 300,,, 2 地沟油检测方法的研究现状,, : 2.1 感官及理化检验法 [2],,,,, [3] 22~ , 1.465,, [4] ( ) 36,,,,, [5] [6],,, 2.2 仪器方法检验,,,, : 色谱法,, [7] (GC), [5], [8] ,, (C12:0) (C14:0) (C17:0), 90%

3 1430 4, [9] [10] LC/MS/MS [11] [12],,, 5% ( ) [13], ( 4) ( 1),, ( ),, 质谱法, 4, 3 [14-16] [17] - (GC-MS) 37, [18] -, [19], [20] (SPME) - (GC-MS), ( ) trans-c18:1 trans-c18:2 3-2,5-,,,,, [21],, 86.5% [14] - - (LC-MS/MS) ( ), [15] /, (13- ), [16],,, 89% 100% 光谱技术, [22-23], [24] 1711 cm -1,, 10% Gonzaga [25], 2900 nm, Kuligowski [26], 2.28% (W/W) Moros [27] [28] [29] [30],, λex/λem

4 5, : 1431 = 230/290 nm,, ; 10% [31], [32], [33], 核磁共振法 ( NMR),, 高场核磁共振法, NMR H, [34] [35] (600 ) H, 12,, 90%,, 低场核磁共振技术 (low-field nuclear magnetic resonance, LF-NMR) 0.5 T [36], LF-NMR,, [37-38],, 其他仪器方法 (DSC), [39] PEN3 Chiavaro [40], DSC, [41] 2.3 其他检测方法,,, 1% 5% 30%, [42] [43] [44],, 3 低场核磁共振法检测地沟油的研究进展 3.1 低场核磁共振的原理 1 H LF-NMR [45-46], ( 1), ( 2), ( - ) ( - ), T 2 T 1,, T 2, [47], T 2, 3.2 低场核磁共振检测的参数 (LF-NMR),,

5 1432 4, [49], T 2,, 3.3 煎炸油的低场核磁共振检测 Fig. 1 1 Nuclear is transformed into cone in processional with magnetic field added LF-NMR, LF-NMR Martna [51],, [50] LF-NMR, T 2 T 21 (S 21 ) T 2W ( ) [52] / ( TPC ) LF-NMR, LF-NMR,, 2 Fig. 2 Resonance absorption and energy level transition [48] [49], LF-NMR, LF-NMR, (TD) (SW) (TR) (NS) (τ) (EchoCount),, [48] [49], T 2, T 2, 32, LF-NMR [50] LF-NMR,,,, 3.4 地沟油及掺伪植物油的低场核磁共振检测 LF-NMR [53] LF-NMR ( ) (SFC), SFC SFC ( 0), [54] T 2, [55],, LF-NMR T 21 (S 21 ) T 21, %, %, 22 78% [50]

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