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1 5 10 Vol. 5 No Journal of Food Safety and Quality Oct., 2014 罗义辉 1, 陈江 2, 王国民 (1., ; 2., ) 2* 摘要 : 目的方法, 结果 Pb As Hg 结论关键词 : ; ; ; Determination of illegal adding of industrial sodium hydroxide in sleeve-fish, sea cucumber and raw milk LUO Yi-Hui 1, CHEN Jiang 2, WANG Guo-Min 2* (1.College of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing , China; 2. Technical Center of Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing , China) ABSTRACT: Objective To detect the illegal using of industrial sodium hydroxide during the food production, such as squid, sea cucumber, fresh milk. Method The protein denaturation in squid and sea cucumber caused by the illegally added industrial sodium hydroxide was determined by differential scanning calorimetry (DSC). The change of heavy metal element content in squid, sea cucumber and fresh milk was measured by atomic absorption spectroscopy (AAS). Results Adding industrial sodium hydroxide could cause protein denaturation as well as the increasing of heavy metal content including Pb, As and Hg in food. Conclusion Both DSC and AAS can be employed as auxiliary methods in monitoring the industrial sodium hydroxide using in food industry. KEY WORDS: industrial sodium hydroxide; differential scanning of calorimetry; atomic absorption spectrometry; food 1 引言,,,,,, 基金项目 : ( ) Fund: Supported by the Special Public Welfare Industry Research of the State Administration of Quality Supervision, Inspection and Quarantine ( ) * 通讯作者 :,,, chqwgm@163.com. *Corresponding author: WANG Guo-Min, Professor, Technical Center of Chongqing Entry-Exit Inspection & Quarantine Bureau, No.8, Honghuang Road, Jiangbei District, Chongqing , China. chqwgm@163.com

2 3256 5,,, Pb As Hg [1],, () ( ), (differential scanning of calorimetry, DSC), [2-5] (atomic absorption spectroscopy, AAS) ( μg /cm 3 ), [6-8], DSC AAS, 2 材料和方法 2.1 仪器与试剂 Q200 ( TA ), TAS-986 () As Pb Hg (, 1000 mg/l), 2.2 实验材料 ( ), ( ) 2.3 实验方法样品处理 2 g, 0, 0.1%, 1.0%, 5.0%, 10.0 %, 20.0% (w:w) 10 ml 4 h 10 ml 1 ml 5, 15~20 mg (DSC) [9,10], DSC 测定,, 30, 10 min, 30~100, 5 /min, 100 ml/min,, AAS 测定重金属含量 : 0.5 g ( ), -(1:4) 4 ml, 4 ml,,, 25 ml,,, [11,12] : 0.5% Pb As Hg, mg/l TAS-986, 5, [13,14] :,, [14,15] 3 结果和讨论 3.1 蛋白质变性 DSC,,,,,,,,, [9,10] DSC, 1 1,,, [2,3]

3 10, : 重金属含量, 2 2,,, 3,,,,,,, Pb, As Hg Pb, 1 Table 1 表 1 不同处理的海参和鱿鱼的变性温度和变性焓 Denaturation temperature and enthalpy of sea cucumbers, and squid in different process (%, w:w) 1( ) 2( ) (J/g) * 1( ) 2( ) (J/g) * * Table 2 表 2 工业火碱处理后样品重金属含量和相对标准偏差 Heavy metal content and the relative standard deviation (RSD) of samples after industrial sodium hydroxide treatment (%, w:w) (mg/kg) RSD(%) (mg/kg) RSD(%) (mg/kg) RSD(%)

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Microsoft Word 聂雪梅.doc

Microsoft Word 聂雪梅.doc 4 4 Vol. 4 No. 4 2013 8 Journal of Food Safety and Quality Aug., 2013 聂雪梅 *, 李立, 孙利, 高飞 (, 100123) 摘要 : 2011 12,, 2007~2011,,,, 关键词 : ; ; ; ; Trade and notification situation on food import and export